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The map label for this gene is tig

Identifier: 229918352

GI number: 229918352

Start: 2601261

End: 2602553

Strand: Direct

Name: tig

Synonym: EAT1b_2637

Alternate gene names: 229918352

Gene position: 2601261-2602553 (Clockwise)

Preceding gene: 229918351

Following gene: 229918353

Centisome position: 86.71

GC content: 47.1

Gene sequence:

>1293_bases
ATGACAGCAAAATGGGAAAAAACATCAGGTAGCCAAGGCGTACTCACGTACGAAGCTCCTGCAGAAGCTTTTGATAAAGC
AGTTGACGCCGCATTTAAAACAGTCGTCAAAGACTTGAACGTGCCTGGCTTCCGTAAAGGGAAATTGCCACGCCCAATGT
TCAACAAAATGTATGGGGAAGAAGCACTCTATCAAGATGCGCTCGACATTCTCTACCGCGACACAATCCAAGGTGCGGTT
GAAGAAGCTGGTTTCGAACCAATCGCGCTCGAAAACATCGATGTAGACGGCACGCTCGAGAAAGGCAAACCAGTCGCTTT
CAAAGTGACATTCGTGGTTGAGCCAGAAGCTGAGCTTGGCGAATACAAAGGCCTCGAGTACGAAGCTGTTTCAACTGAAG
TAACAGAAGACGAAGTAACAGCAGAACTTGAAGCACTTCAACAGCAAGGCGCAGAACTCGCTGTTAAAGAAGGTGCAATC
GAAAACGGCGATACAGCTGTATTTGATTTCGCAGGTTTCGCTGACGGTGAGCAGTTCGAAGGCGGCACAGCTGAGAACTA
CACACTCGAAATCGGTTCTGGTCAATTCATCCCAGGATTCGAAGAGCAAATGATCGGCATGACTGCTGGTGAAGAAAAAG
ACGTTGAAGTAACATTCCCTGAAGAGTACCACGCTGAAAACTTGGCGGGTAAACCAGCGACATTCAAAGTCAAACTTCAC
GAAGTCAAAACAAAACAAGTTCCTGAACTCGACGACGAGTTTGCAAAAGACATCGACGAGTCTGTTTCGACACTCGACGA
GTTGAAAGCAAGCATCCGTGAGCGTTTAGAAGCTGGTAAGAAACAAGAAGCTGAAGGTACAATGCGCGACCAACTCGTAG
AGCAGGCAACTGCTAACGCGACAATCGACGTACCTGAAGTTCTCGTTGAACAAGAAGTAGACCGCATGGTACAAGAGTTC
GGTTCACGTGTATCTTCACAAGGAATCGACCTTAACATGTACTTCGAACTCACTGGTACTTCTGAAGAAGCAATGCGCGA
AGAGATGAAAGAGCAAGCTGAAGAGCGCGTAAAAGCTCGTCTCGTCTTGAAAGCAATCGCTGACAAAGAAGCAATCGAAG
TATCTGACGAAGATGCTGAGAAAGAGCTTGAAGAAATGTCTAAACTTTACAACATCGCACCGGACCAACTCCGTACGATG
CTCGCTCCGCAAGGCGGACTTGAGACACTTAAAGGTGACCTCAAATTCCGTAAAGCGATCGACATCCTCGTTGAAAACGG
TAAAGCGAAGTAA

Upstream 100 bases:

>100_bases
TATTAGACTAAATCCTTGTGTGCTTACCGAGGATTTATGCTATAATACGGATTGTTGTACATTTTTGTACGGTATAAACG
AATTGTTGGAGGGACTTAGC

Downstream 100 bases:

>100_bases
GCTGATATTCGAAGGGACGACTGCCTAGCGGTCGTCCTTTTTTCTGTCACAGCACATTGCATGTGTAATTCTTTGAAGTC
GCAATATGTTCATGCTACAA

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MTAKWEKTSGSQGVLTYEAPAEAFDKAVDAAFKTVVKDLNVPGFRKGKLPRPMFNKMYGEEALYQDALDILYRDTIQGAV
EEAGFEPIALENIDVDGTLEKGKPVAFKVTFVVEPEAELGEYKGLEYEAVSTEVTEDEVTAELEALQQQGAELAVKEGAI
ENGDTAVFDFAGFADGEQFEGGTAENYTLEIGSGQFIPGFEEQMIGMTAGEEKDVEVTFPEEYHAENLAGKPATFKVKLH
EVKTKQVPELDDEFAKDIDESVSTLDELKASIRERLEAGKKQEAEGTMRDQLVEQATANATIDVPEVLVEQEVDRMVQEF
GSRVSSQGIDLNMYFELTGTSEEAMREEMKEQAEERVKARLVLKAIADKEAIEVSDEDAEKELEEMSKLYNIAPDQLRTM
LAPQGGLETLKGDLKFRKAIDILVENGKAK

Sequences:

>Translated_430_residues
MTAKWEKTSGSQGVLTYEAPAEAFDKAVDAAFKTVVKDLNVPGFRKGKLPRPMFNKMYGEEALYQDALDILYRDTIQGAV
EEAGFEPIALENIDVDGTLEKGKPVAFKVTFVVEPEAELGEYKGLEYEAVSTEVTEDEVTAELEALQQQGAELAVKEGAI
ENGDTAVFDFAGFADGEQFEGGTAENYTLEIGSGQFIPGFEEQMIGMTAGEEKDVEVTFPEEYHAENLAGKPATFKVKLH
EVKTKQVPELDDEFAKDIDESVSTLDELKASIRERLEAGKKQEAEGTMRDQLVEQATANATIDVPEVLVEQEVDRMVQEF
GSRVSSQGIDLNMYFELTGTSEEAMREEMKEQAEERVKARLVLKAIADKEAIEVSDEDAEKELEEMSKLYNIAPDQLRTM
LAPQGGLETLKGDLKFRKAIDILVENGKAK
>Mature_429_residues
TAKWEKTSGSQGVLTYEAPAEAFDKAVDAAFKTVVKDLNVPGFRKGKLPRPMFNKMYGEEALYQDALDILYRDTIQGAVE
EAGFEPIALENIDVDGTLEKGKPVAFKVTFVVEPEAELGEYKGLEYEAVSTEVTEDEVTAELEALQQQGAELAVKEGAIE
NGDTAVFDFAGFADGEQFEGGTAENYTLEIGSGQFIPGFEEQMIGMTAGEEKDVEVTFPEEYHAENLAGKPATFKVKLHE
VKTKQVPELDDEFAKDIDESVSTLDELKASIRERLEAGKKQEAEGTMRDQLVEQATANATIDVPEVLVEQEVDRMVQEFG
SRVSSQGIDLNMYFELTGTSEEAMREEMKEQAEERVKARLVLKAIADKEAIEVSDEDAEKELEEMSKLYNIAPDQLRTML
APQGGLETLKGDLKFRKAIDILVENGKAK

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=394, Percent_Identity=33.248730964467, Blast_Score=194, Evalue=7e-51,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_EXISA (C4L4J0)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002886998.1
- GeneID:   7869650
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_2637
- OMA:   RFIPGFE
- ProtClustDB:   CLSK2489686
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PANTHER:   PTHR10516
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 47533; Mature: 47401

Theoretical pI: Translated: 4.07; Mature: 4.07

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKWEKTSGSQGVLTYEAPAEAFDKAVDAAFKTVVKDLNVPGFRKGKLPRPMFNKMYGE
CCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCH
EALYQDALDILYRDTIQGAVEEAGFEPIALENIDVDGTLEKGKPVAFKVTFVVEPEAELG
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCEEEEEEEEECCCHHHH
EYKGLEYEAVSTEVTEDEVTAELEALQQQGAELAVKEGAIENGDTAVFDFAGFADGEQFE
HCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEECCCCCCCCCC
GGTAENYTLEIGSGQFIPGFEEQMIGMTAGEEKDVEVTFPEEYHAENLAGKPATFKVKLH
CCCCCCEEEEECCCCCCCCHHHHHHCCCCCCCCCEEEECCHHHHHHHCCCCCCEEEEEEE
EVKTKQVPELDDEFAKDIDESVSTLDELKASIRERLEAGKKQEAEGTMRDQLVEQATANA
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCHHHHHHHHHHHHCCC
TIDVPEVLVEQEVDRMVQEFGSRVSSQGIDLNMYFELTGTSEEAMREEMKEQAEERVKAR
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHH
LVLKAIADKEAIEVSDEDAEKELEEMSKLYNIAPDQLRTMLAPQGGLETLKGDLKFRKAI
HHHHHHHCCHHEECCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHH
DILVENGKAK
HHHHCCCCCC
>Mature Secondary Structure 
TAKWEKTSGSQGVLTYEAPAEAFDKAVDAAFKTVVKDLNVPGFRKGKLPRPMFNKMYGE
CCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCH
EALYQDALDILYRDTIQGAVEEAGFEPIALENIDVDGTLEKGKPVAFKVTFVVEPEAELG
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCEEEEEEEEECCCHHHH
EYKGLEYEAVSTEVTEDEVTAELEALQQQGAELAVKEGAIENGDTAVFDFAGFADGEQFE
HCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEECCCCCCCCCC
GGTAENYTLEIGSGQFIPGFEEQMIGMTAGEEKDVEVTFPEEYHAENLAGKPATFKVKLH
CCCCCCEEEEECCCCCCCCHHHHHHCCCCCCCCCEEEECCHHHHHHHCCCCCCEEEEEEE
EVKTKQVPELDDEFAKDIDESVSTLDELKASIRERLEAGKKQEAEGTMRDQLVEQATANA
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCHHHHHHHHHHHHCCC
TIDVPEVLVEQEVDRMVQEFGSRVSSQGIDLNMYFELTGTSEEAMREEMKEQAEERVKAR
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHH
LVLKAIADKEAIEVSDEDAEKELEEMSKLYNIAPDQLRTMLAPQGGLETLKGDLKFRKAI
HHHHHHHCCHHEECCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHH
DILVENGKAK
HHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA