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The map label for this gene is rph [H]

Identifier: 229918348

GI number: 229918348

Start: 2598147

End: 2598896

Strand: Direct

Name: rph [H]

Synonym: EAT1b_2633

Alternate gene names: 229918348

Gene position: 2598147-2598896 (Clockwise)

Preceding gene: 229918347

Following gene: 229918349

Centisome position: 86.61

GC content: 54.8

Gene sequence:

>750_bases
TTGCGTGATGCGAGACGATTTGATGAACTGCGCCCAGTCGAGATTGTGCCGCACGTGAACAAGCATGCGGAAGGGTCCGT
ATTGATTTCAATCGGCGATACGAAAGTCATCTGTACCGCGACCGTTGAGGAACGGGTACCGAATTTCCTTCGCGGAAAGA
AACAAGGGTGGATCAATGCAGAATATGCGATGCTCCCACGGGCGACGGGAGACCGTACTGTCCGGGAATCGGTACGTGGA
AAACAATCGGGACGAACGATGGAGATTCAGCGTTTGATTGGCCGGTCGCTCCGCTCGGTCGTGGACTTAGAACGTCTCGG
TGAACGGACGATTTGGGTCGACTGTGACGTCATTCAAGCGGATGGCGGCACTCGGACGGCTTCCATCACAGGTGGCTTTT
GCGCGCTCGTGTTGGCCGTCGATCGCTTGATTCGCCAAGGGAAACTTGAAGATACACCGATTAAAGAAGGTGTGGCTGCG
ATTTCTGTCGGGCGAACGGACGAGCTTGTCTTGGACTTGGATTACCTAGAAGATGCCGCAGCAGAGGTCGACATGAATGT
CGTCATGACGGCAAGCGGTCGGTTCGTCGAAGTACAAGGTACAGGGGAAGAGGCGACGTTCACGCTACAAGAGATGAACG
AGATGCTCGCACTCGCGCAACACGGCATCGAATCATTATTCAAGACGGCGGAAGCGGCACTCGGTGCTTCTTGGTGGTAC
GTCGGGGAACGTTCGGAGGAGACATTATGA

Upstream 100 bases:

>100_bases
CATGCCTTCTATGCGACGGGTGATATCGAACTATTTGACCGACTCGCGAGCGAATGGCTCGGGCGTCCGGTTCATGCGGA
GAAAGTCGAGGTGACCGGCA

Downstream 100 bases:

>100_bases
AGTTGATCATTGCGACACACAATCCTGGAAAAGTGAAAGAACTCGAAGGGATGTTGACGCCGCTCGGATTCGAGGTCGAG
TCACTCCTCGACTATCCGGA

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MRDARRFDELRPVEIVPHVNKHAEGSVLISIGDTKVICTATVEERVPNFLRGKKQGWINAEYAMLPRATGDRTVRESVRG
KQSGRTMEIQRLIGRSLRSVVDLERLGERTIWVDCDVIQADGGTRTASITGGFCALVLAVDRLIRQGKLEDTPIKEGVAA
ISVGRTDELVLDLDYLEDAAAEVDMNVVMTASGRFVEVQGTGEEATFTLQEMNEMLALAQHGIESLFKTAEAALGASWWY
VGERSEETL

Sequences:

>Translated_249_residues
MRDARRFDELRPVEIVPHVNKHAEGSVLISIGDTKVICTATVEERVPNFLRGKKQGWINAEYAMLPRATGDRTVRESVRG
KQSGRTMEIQRLIGRSLRSVVDLERLGERTIWVDCDVIQADGGTRTASITGGFCALVLAVDRLIRQGKLEDTPIKEGVAA
ISVGRTDELVLDLDYLEDAAAEVDMNVVMTASGRFVEVQGTGEEATFTLQEMNEMLALAQHGIESLFKTAEAALGASWWY
VGERSEETL
>Mature_249_residues
MRDARRFDELRPVEIVPHVNKHAEGSVLISIGDTKVICTATVEERVPNFLRGKKQGWINAEYAMLPRATGDRTVRESVRG
KQSGRTMEIQRLIGRSLRSVVDLERLGERTIWVDCDVIQADGGTRTASITGGFCALVLAVDRLIRQGKLEDTPIKEGVAA
ISVGRTDELVLDLDYLEDAAAEVDMNVVMTASGRFVEVQGTGEEATFTLQEMNEMLALAQHGIESLFKTAEAALGASWWY
VGERSEETL

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=210, Percent_Identity=61.4285714285714, Blast_Score=256, Evalue=1e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 27480; Mature: 27480

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDARRFDELRPVEIVPHVNKHAEGSVLISIGDTKVICTATVEERVPNFLRGKKQGWINA
CCCHHHHHCCCCEEEECCCCCCCCCEEEEEECCCEEEEEEEHHHHCHHHHCCCCCCCCCC
EYAMLPRATGDRTVRESVRGKQSGRTMEIQRLIGRSLRSVVDLERLGERTIWVDCDVIQA
CEEECCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEEC
DGGTRTASITGGFCALVLAVDRLIRQGKLEDTPIKEGVAAISVGRTDELVLDLDYLEDAA
CCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEECCCCCCEEEEHHHHHHHH
AEVDMNVVMTASGRFVEVQGTGEEATFTLQEMNEMLALAQHGIESLFKTAEAALGASWWY
HHCCCEEEEECCCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
VGERSEETL
ECCCCCCCC
>Mature Secondary Structure
MRDARRFDELRPVEIVPHVNKHAEGSVLISIGDTKVICTATVEERVPNFLRGKKQGWINA
CCCHHHHHCCCCEEEECCCCCCCCCEEEEEECCCEEEEEEEHHHHCHHHHCCCCCCCCCC
EYAMLPRATGDRTVRESVRGKQSGRTMEIQRLIGRSLRSVVDLERLGERTIWVDCDVIQA
CEEECCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEEC
DGGTRTASITGGFCALVLAVDRLIRQGKLEDTPIKEGVAAISVGRTDELVLDLDYLEDAA
CCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEECCCCCCEEEEHHHHHHHH
AEVDMNVVMTASGRFVEVQGTGEEATFTLQEMNEMLALAQHGIESLFKTAEAALGASWWY
HHCCCEEEEECCCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
VGERSEETL
ECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA