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The map label for this gene is ptsH [H]

Identifier: 229918346

GI number: 229918346

Start: 2596975

End: 2597238

Strand: Direct

Name: ptsH [H]

Synonym: EAT1b_2631

Alternate gene names: 229918346

Gene position: 2596975-2597238 (Clockwise)

Preceding gene: 229918345

Following gene: 229918347

Centisome position: 86.57

GC content: 43.56

Gene sequence:

>264_bases
ATGGAAAAAACGTTCACAGTCGTTGCTGATTCAGGAATTCACGCTCGTCCAGCTACTCAGTTGGTTAACACAGCTTCTAA
GTTCCAGTCAGACATCAACCTCGAGTACAATGGTAAAACAGTTAACTTGAAGTCGATCATGGGTGTACTTTCACTCGGTA
TCGCAAAAGATTCTACAATCAAGATCACTGCTGAAGGTGAAGATGCAGAAGAAGCGATCAACACGCTTTCTGACATGCTC
ACTAGCGAAGGTCTTGCTCAGTAA

Upstream 100 bases:

>100_bases
ATTTTTGCGAATCCGCTATCATTTGATAATGAGTTCACAAACAAAAAATATTCTGCTGGTTCGCCTGTAGAATTGAATTT
TGAAGGGAGACCATCATCTC

Downstream 100 bases:

>100_bases
TTCGGGTAAGGGACGAGGCGCCACTGGCGACCTCGTCTTTTTTTGTCATGGGAACGTTTCTTTTTCCGTCCGAGGTTTTT
TGGTAAGATAGGTGAGAATG

Product: phosphotransferase system, phosphocarrier protein HPr

Products: fructose-6-phosphate [Cytoplasm]; pyruvate; fructose-1-phosphate [Cytoplasm] [C]

Alternate protein names: Histidine-containing protein [H]

Number of amino acids: Translated: 87; Mature: 87

Protein sequence:

>87_residues
MEKTFTVVADSGIHARPATQLVNTASKFQSDINLEYNGKTVNLKSIMGVLSLGIAKDSTIKITAEGEDAEEAINTLSDML
TSEGLAQ

Sequences:

>Translated_87_residues
MEKTFTVVADSGIHARPATQLVNTASKFQSDINLEYNGKTVNLKSIMGVLSLGIAKDSTIKITAEGEDAEEAINTLSDML
TSEGLAQ
>Mature_87_residues
MEKTFTVVADSGIHARPATQLVNTASKFQSDINLEYNGKTVNLKSIMGVLSLGIAKDSTIKITAEGEDAEEAINTLSDML
TSEGLAQ

Specific function: P-Ser-HPr interacts with the catabolite control protein A (CcpA), forming a complex that binds to DNA at the catabolite response elements cre, operator sites preceding a large number of catabolite-regulated genes. Thus, P-Ser-HPr is a corepressor in carbo

COG id: COG1925

COG function: function code G; Phosphotransferase system, HPr-related proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HPr domain [H]

Homologues:

Organism=Escherichia coli, GI1788494, Length=81, Percent_Identity=43.2098765432099, Blast_Score=73, Evalue=3e-15,
Organism=Escherichia coli, GI1788755, Length=80, Percent_Identity=35, Blast_Score=64, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114 [H]

Pfam domain/function: PF00381 PTS-HPr [H]

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 9245; Mature: 9245

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS00369 PTS_HPR_HIS ; PS00589 PTS_HPR_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKTFTVVADSGIHARPATQLVNTASKFQSDINLEYNGKTVNLKSIMGVLSLGIAKDSTI
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEECCCEEEHHHHHHHHHHCCCCCCEE
KITAEGEDAEEAINTLSDMLTSEGLAQ
EEEECCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MEKTFTVVADSGIHARPATQLVNTASKFQSDINLEYNGKTVNLKSIMGVLSLGIAKDSTI
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEECCCEEEHHHHHHHHHHCCCCCCEE
KITAEGEDAEEAINTLSDMLTSEGLAQ
EEEECCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Fructose [Periplasm]; phosphoenolpyruvate [C]

Specific reaction: phosphoenolpyruvate + fructose [Periplasm] = fructose-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + fructose [Periplasm] = fructose-1-phosphate [Cytoplasm] + pyruvate [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA