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The map label for this gene is iscS [H]

Identifier: 229918304

GI number: 229918304

Start: 2554397

End: 2555515

Strand: Reverse

Name: iscS [H]

Synonym: EAT1b_2588

Alternate gene names: 229918304

Gene position: 2555515-2554397 (Counterclockwise)

Preceding gene: 229918305

Following gene: 229918300

Centisome position: 85.19

GC content: 39.14

Gene sequence:

>1119_bases
ATGCTTTATCTAGACAACAGCTCAACGACACCTGTGTATCCAGAAGTACTTGAAGAGATGCTCCCCTACTTTCTTGAAGA
GTTCGGAAATCCTTCGAGCAAGTATTATTCACTGGCAGAAAACTCTAAAAATGCTGTTGAACATGCTCGAGAACAGTTAG
GTAAATTTCTTAACTGTTCTTCTACCGAGCTGTATTTTACTTCGGGAGCGACAGAAAGTAACAACTGGATTTTAAAGAGT
GTAAGTTCAACCTTACCGGATCATCAAGTGATTACCACGGTAGCCGAACATTCATCTAATTTAGAAATCGCAAAGTATTT
AAATGAGCAGCTGAATATTCACGTTGAATACTTGTCAGTCGATCAATTTGGTAGAATCAATTTTTCTGAACTAGAGTCTT
TACTAAAAAAACGCCCTACTTCACTCGTTTCAATCATTTGGGGAAATAACGAGTTAGGGTCGTTAAATGACATTCAGAGA
GCTTCTGAACTAGTTGCTCAATATGGTGCGCTCTTTCACACCGATGCCACACATGTCGTCGGAAAGATGAGAATCGACCT
GTCAGAACTGACGAACGTTCATTTCTTATCCGCTTCTGCGCATAAGTTCAACGGTCCAAAAGGTATTGGAATTGCTTATT
TGAAAAAAGATGAGGACGGGATTCCAAGAAAATTGACACCACTTATTCATGGTGGTGGCCAAGAGCAAGGCATTCGAGGC
GGGACTCTTGCTGTTCCTCTTATTGTAGGAATGGGAAAAGCAGCTGAAATGCATCAAGCATCCTTGACCAAGAACATCAA
AAAATTAGAGAAACTTGAAGCGTATTTAGTTCAACTTTTAACTCAAAAATTTGGTTCGAACATACGATTTAATAGTGACC
ATACAAACAAAATTCCTGGCATTGTGAATATTCAATTCGTTGGTATTAACAATGAAGTTCTTGTAAAACAACTGGCTCCT
GTCATGGCGGTCTCAACTGGATCTGCTTGTAGCTCTAGTAAACCAAGCCATGTCCTCCAAGCAATCGGATTAACATTAGA
TGAAGTTCGATCATCCATTCGATTCTCACTATCGTCAAACATTGAACTAAAAGACTTAGAGATTTTCAAAGAACTGTAA

Upstream 100 bases:

>100_bases
CATATTACTCGAGGTGCCGAAGAGCTTTATCAAATGTTCCAATTTTCTAGAAGTACCAATCAATTCTTAAGAGAGTTATT
GGCTCTCCAGGAGGGATAAC

Downstream 100 bases:

>100_bases
GTAAACGACCGGCTTAAATTAAATTTAAGCCGGTCGTTTTAAGTTCAAATATTATTAATTTTGAAGGATTCAAGCATTTC
TTCTCTTGGAATATCCTTTT

Product: Cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MLYLDNSSTTPVYPEVLEEMLPYFLEEFGNPSSKYYSLAENSKNAVEHAREQLGKFLNCSSTELYFTSGATESNNWILKS
VSSTLPDHQVITTVAEHSSNLEIAKYLNEQLNIHVEYLSVDQFGRINFSELESLLKKRPTSLVSIIWGNNELGSLNDIQR
ASELVAQYGALFHTDATHVVGKMRIDLSELTNVHFLSASAHKFNGPKGIGIAYLKKDEDGIPRKLTPLIHGGGQEQGIRG
GTLAVPLIVGMGKAAEMHQASLTKNIKKLEKLEAYLVQLLTQKFGSNIRFNSDHTNKIPGIVNIQFVGINNEVLVKQLAP
VMAVSTGSACSSSKPSHVLQAIGLTLDEVRSSIRFSLSSNIELKDLEIFKEL

Sequences:

>Translated_372_residues
MLYLDNSSTTPVYPEVLEEMLPYFLEEFGNPSSKYYSLAENSKNAVEHAREQLGKFLNCSSTELYFTSGATESNNWILKS
VSSTLPDHQVITTVAEHSSNLEIAKYLNEQLNIHVEYLSVDQFGRINFSELESLLKKRPTSLVSIIWGNNELGSLNDIQR
ASELVAQYGALFHTDATHVVGKMRIDLSELTNVHFLSASAHKFNGPKGIGIAYLKKDEDGIPRKLTPLIHGGGQEQGIRG
GTLAVPLIVGMGKAAEMHQASLTKNIKKLEKLEAYLVQLLTQKFGSNIRFNSDHTNKIPGIVNIQFVGINNEVLVKQLAP
VMAVSTGSACSSSKPSHVLQAIGLTLDEVRSSIRFSLSSNIELKDLEIFKEL
>Mature_372_residues
MLYLDNSSTTPVYPEVLEEMLPYFLEEFGNPSSKYYSLAENSKNAVEHAREQLGKFLNCSSTELYFTSGATESNNWILKS
VSSTLPDHQVITTVAEHSSNLEIAKYLNEQLNIHVEYLSVDQFGRINFSELESLLKKRPTSLVSIIWGNNELGSLNDIQR
ASELVAQYGALFHTDATHVVGKMRIDLSELTNVHFLSASAHKFNGPKGIGIAYLKKDEDGIPRKLTPLIHGGGQEQGIRG
GTLAVPLIVGMGKAAEMHQASLTKNIKKLEKLEAYLVQLLTQKFGSNIRFNSDHTNKIPGIVNIQFVGINNEVLVKQLAP
VMAVSTGSACSSSKPSHVLQAIGLTLDEVRSSIRFSLSSNIELKDLEIFKEL

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=361, Percent_Identity=37.9501385041551, Blast_Score=237, Evalue=1e-62,
Organism=Homo sapiens, GI156713448, Length=408, Percent_Identity=32.3529411764706, Blast_Score=171, Evalue=1e-42,
Organism=Escherichia coli, GI48994898, Length=363, Percent_Identity=38.2920110192837, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1787970, Length=289, Percent_Identity=23.5294117647059, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1789175, Length=349, Percent_Identity=23.2091690544413, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25143064, Length=362, Percent_Identity=38.121546961326, Blast_Score=241, Evalue=6e-64,
Organism=Caenorhabditis elegans, GI17533177, Length=325, Percent_Identity=26.4615384615385, Blast_Score=114, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6319831, Length=362, Percent_Identity=39.2265193370166, Blast_Score=235, Evalue=9e-63,
Organism=Drosophila melanogaster, GI20129463, Length=361, Percent_Identity=39.0581717451524, Blast_Score=227, Evalue=1e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 41049; Mature: 41049

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYLDNSSTTPVYPEVLEEMLPYFLEEFGNPSSKYYSLAENSKNAVEHAREQLGKFLNCS
CEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHEEHHCCCHHHHHHHHHHHHHHHCCC
STELYFTSGATESNNWILKSVSSTLPDHQVITTVAEHSSNLEIAKYLNEQLNIHVEYLSV
CCEEEEECCCCCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHEEEEEEEEE
DQFGRINFSELESLLKKRPTSLVSIIWGNNELGSLNDIQRASELVAQYGALFHTDATHVV
CCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHH
GKMRIDLSELTNVHFLSASAHKFNGPKGIGIAYLKKDEDGIPRKLTPLIHGGGQEQGIRG
HHHEEEHHHHCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCCHHCHHHCCCCCCCCCCC
GTLAVPLIVGMGKAAEMHQASLTKNIKKLEKLEAYLVQLLTQKFGSNIRFNSDHTNKIPG
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCC
IVNIQFVGINNEVLVKQLAPVMAVSTGSACSSSKPSHVLQAIGLTLDEVRSSIRFSLSSN
EEEEEEEECCCHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHEEHHCCC
IELKDLEIFKEL
CEEHHHHHHHCC
>Mature Secondary Structure
MLYLDNSSTTPVYPEVLEEMLPYFLEEFGNPSSKYYSLAENSKNAVEHAREQLGKFLNCS
CEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHEEHHCCCHHHHHHHHHHHHHHHCCC
STELYFTSGATESNNWILKSVSSTLPDHQVITTVAEHSSNLEIAKYLNEQLNIHVEYLSV
CCEEEEECCCCCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHEEEEEEEEE
DQFGRINFSELESLLKKRPTSLVSIIWGNNELGSLNDIQRASELVAQYGALFHTDATHVV
CCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHH
GKMRIDLSELTNVHFLSASAHKFNGPKGIGIAYLKKDEDGIPRKLTPLIHGGGQEQGIRG
HHHEEEHHHHCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCCHHCHHHCCCCCCCCCCC
GTLAVPLIVGMGKAAEMHQASLTKNIKKLEKLEAYLVQLLTQKFGSNIRFNSDHTNKIPG
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCC
IVNIQFVGINNEVLVKQLAPVMAVSTGSACSSSKPSHVLQAIGLTLDEVRSSIRFSLSSN
EEEEEEEECCCHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHEEHHCCC
IELKDLEIFKEL
CEEHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA