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The map label for this gene is nplT [H]

Identifier: 229918221

GI number: 229918221

Start: 2463828

End: 2465612

Strand: Reverse

Name: nplT [H]

Synonym: EAT1b_2501

Alternate gene names: 229918221

Gene position: 2465612-2463828 (Counterclockwise)

Preceding gene: 229918230

Following gene: 229918220

Centisome position: 82.19

GC content: 51.15

Gene sequence:

>1785_bases
ATGATGAAAGAAGCGATTTATCATCGTGCCATGTCCCCGTTCGTCTACAAATATGATCGTGAGACGATCCATATTCGTTT
CCATACGAAGCGAGGCGATATCGAGTCGGTCGATTTGATTTGGAACGACCCATATGATTGGCACGCTGAAGACCCGGCCA
TGTGGAACTTCGATCCGGACAAGCCGAGCTACTGGCGTACATACGAGACTCCAATGGAGAAGATCGGATTTGACGACGCC
TATGACTATTGGTTCATCGCCATCAAACCGCCGTTCCGACGTCTACGTTACGGTTTCCGTTTGAATGATGGACAAGAGAC
GGCCGTCTACACTGAAAAAGGATGGTTCGAGGAGAAGCCGCTCGATGATACGGGCTACTACTTCTGTGTCCCATTCATCA
ACCCGATCGATATCTTCCATGCACCTTCCTGGGTGAAGGATACGGTCTGGTATCAAATCTTCCCGGACCGTTTCGCCAAT
GGCGATGCTTCACTCAATCCTGAAGAGACGCTCCCATGGAACTCGACAGAGCCGACGACGACAAACTTCTTCGGTGGCGA
CTTCCAAGGGGTGATCGACCATATCGATCATTTAGTCGAACTCGGGATCACCGGCATCTACTTCTGCCCGATCTTTAAAG
CGACGACGAACCATAAGTACGATACGCTCGACTACATGGAAATCGATCCGCAGTTCGGGACGAAAGAGAAATTTAAAGAG
ATGGTCGACCTCTTGCACGAGAACGGCATCAAAGTCATGCTCGACGCGGTCTTCAACCATGTCGGATATCATCACCCATG
GTTCCAAGACGTCGTCGAGAACGGACCGTCTTCTGAGTATCGCGATTGGTTCTATCTTCGCGAATTCCCGATTGCGACAG
AGCCGAAGCCGAACTACGACACCTTCGCATTCGAAAAGAACATGCCTAAGATCAATACAGAACATCCAGAGTTAAAAGCT
TACTTATTAGATGTCGCCCGTTACTGGGTCGAAGAATTCGGTATCGACGGATGGCGTCTTGACGTCGCTAACGAAGTCGA
CCATGCGTTTTGGCGTGACTTCCGCAAGGCCGTCAAAGCTGCGAACCCGGAAGCCTACATCCTCGGGGAGATTTGGCACG
ACGCACAGCCTTGGTTACAAGGTGACCAGTTCGATGCCGTCATGAACTACCCGTTCACGAATGCGAGCTTGAACTATTTC
GCCCTCGACCGGACGACCGCTTCGGAGTTCCGTAACGAACTGACGCGTGTCGAGATGATGAACACGATGAACGTCAACGA
AGTCACCTTCAACTTGATCGATTCGCACGATACACCTCGTGCGTTGACACGGGCGAAAGGACATAAAGGCAAGTTCAAAC
TCTTGATGACGTCGATGCTGACGTATACGGGTAGCCCATGTATTTACTACGGGGACGAAATCGGACTCGAAGGCGAACAA
GACCCGGGTTGCCGTCGCTGTATGCCATGGGATGAGGCAGAAGAAGACCGTAACCTCTTCCGCTACATCCAAAAGCTCAT
CCAGCTTCGTAAGGACCACCCTGTGCTCGCCAATGCCGGCTCATATCGCTTCAACCTGGTCGACGATGCGAACAATGCGG
TCATCATCGAGCGGTGTACGAAAGATGAGACATACTTGGTTTACTTCAACAACTCAGACTCTGCCTCGAAACTGAATCCG
CTCGGTTATACAGGAGCCGCCACGGACCTCTTGCATGACCGCGACCTCGACTCACTCGAAATCGAACTGGCGCCATTCAG
TGCTGCTATTCTGAAATTGAAATAA

Upstream 100 bases:

>100_bases
TAACCTTTGCTTGAGCAAACGTTTTCGCAACATCTGTTCTCATGGATTCCCGATGTTGTAAAATGATGTCATCCCCTATA
AAGAAAGGAATGTTTTCCTT

Downstream 100 bases:

>100_bases
ATTCGAAAATCACCTGATTTCTTCGGGTGATTTTTTTGTTGAAAATAATTTAGCAAACCCCTTTCTTTTTCATTTCATAA
GGAGTAAGATGACCTTTGAC

Product: alpha amylase catalytic region

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 594; Mature: 594

Protein sequence:

>594_residues
MMKEAIYHRAMSPFVYKYDRETIHIRFHTKRGDIESVDLIWNDPYDWHAEDPAMWNFDPDKPSYWRTYETPMEKIGFDDA
YDYWFIAIKPPFRRLRYGFRLNDGQETAVYTEKGWFEEKPLDDTGYYFCVPFINPIDIFHAPSWVKDTVWYQIFPDRFAN
GDASLNPEETLPWNSTEPTTTNFFGGDFQGVIDHIDHLVELGITGIYFCPIFKATTNHKYDTLDYMEIDPQFGTKEKFKE
MVDLLHENGIKVMLDAVFNHVGYHHPWFQDVVENGPSSEYRDWFYLREFPIATEPKPNYDTFAFEKNMPKINTEHPELKA
YLLDVARYWVEEFGIDGWRLDVANEVDHAFWRDFRKAVKAANPEAYILGEIWHDAQPWLQGDQFDAVMNYPFTNASLNYF
ALDRTTASEFRNELTRVEMMNTMNVNEVTFNLIDSHDTPRALTRAKGHKGKFKLLMTSMLTYTGSPCIYYGDEIGLEGEQ
DPGCRRCMPWDEAEEDRNLFRYIQKLIQLRKDHPVLANAGSYRFNLVDDANNAVIIERCTKDETYLVYFNNSDSASKLNP
LGYTGAATDLLHDRDLDSLEIELAPFSAAILKLK

Sequences:

>Translated_594_residues
MMKEAIYHRAMSPFVYKYDRETIHIRFHTKRGDIESVDLIWNDPYDWHAEDPAMWNFDPDKPSYWRTYETPMEKIGFDDA
YDYWFIAIKPPFRRLRYGFRLNDGQETAVYTEKGWFEEKPLDDTGYYFCVPFINPIDIFHAPSWVKDTVWYQIFPDRFAN
GDASLNPEETLPWNSTEPTTTNFFGGDFQGVIDHIDHLVELGITGIYFCPIFKATTNHKYDTLDYMEIDPQFGTKEKFKE
MVDLLHENGIKVMLDAVFNHVGYHHPWFQDVVENGPSSEYRDWFYLREFPIATEPKPNYDTFAFEKNMPKINTEHPELKA
YLLDVARYWVEEFGIDGWRLDVANEVDHAFWRDFRKAVKAANPEAYILGEIWHDAQPWLQGDQFDAVMNYPFTNASLNYF
ALDRTTASEFRNELTRVEMMNTMNVNEVTFNLIDSHDTPRALTRAKGHKGKFKLLMTSMLTYTGSPCIYYGDEIGLEGEQ
DPGCRRCMPWDEAEEDRNLFRYIQKLIQLRKDHPVLANAGSYRFNLVDDANNAVIIERCTKDETYLVYFNNSDSASKLNP
LGYTGAATDLLHDRDLDSLEIELAPFSAAILKLK
>Mature_594_residues
MMKEAIYHRAMSPFVYKYDRETIHIRFHTKRGDIESVDLIWNDPYDWHAEDPAMWNFDPDKPSYWRTYETPMEKIGFDDA
YDYWFIAIKPPFRRLRYGFRLNDGQETAVYTEKGWFEEKPLDDTGYYFCVPFINPIDIFHAPSWVKDTVWYQIFPDRFAN
GDASLNPEETLPWNSTEPTTTNFFGGDFQGVIDHIDHLVELGITGIYFCPIFKATTNHKYDTLDYMEIDPQFGTKEKFKE
MVDLLHENGIKVMLDAVFNHVGYHHPWFQDVVENGPSSEYRDWFYLREFPIATEPKPNYDTFAFEKNMPKINTEHPELKA
YLLDVARYWVEEFGIDGWRLDVANEVDHAFWRDFRKAVKAANPEAYILGEIWHDAQPWLQGDQFDAVMNYPFTNASLNYF
ALDRTTASEFRNELTRVEMMNTMNVNEVTFNLIDSHDTPRALTRAKGHKGKFKLLMTSMLTYTGSPCIYYGDEIGLEGEQ
DPGCRRCMPWDEAEEDRNLFRYIQKLIQLRKDHPVLANAGSYRFNLVDDANNAVIIERCTKDETYLVYFNNSDSASKLNP
LGYTGAATDLLHDRDLDSLEIELAPFSAAILKLK

Specific function: Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=396, Percent_Identity=23.4848484848485, Blast_Score=93, Evalue=8e-19,
Organism=Escherichia coli, GI1786604, Length=519, Percent_Identity=30.4431599229287, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1790687, Length=391, Percent_Identity=25.5754475703325, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1789995, Length=135, Percent_Identity=28.1481481481481, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI32565753, Length=218, Percent_Identity=24.3119266055046, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322245, Length=461, Percent_Identity=21.4750542299349, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6319776, Length=402, Percent_Identity=21.3930348258706, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6321731, Length=402, Percent_Identity=21.1442786069652, Blast_Score=87, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6321726, Length=462, Percent_Identity=21.2121212121212, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6324416, Length=464, Percent_Identity=20.2586206896552, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6322241, Length=464, Percent_Identity=20.2586206896552, Blast_Score=77, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6322021, Length=464, Percent_Identity=20.2586206896552, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24586587, Length=400, Percent_Identity=23, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24583747, Length=227, Percent_Identity=29.5154185022026, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24583749, Length=227, Percent_Identity=29.5154185022026, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24586591, Length=233, Percent_Identity=27.8969957081545, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24583745, Length=583, Percent_Identity=23.6706689536878, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24586593, Length=403, Percent_Identity=23.8213399503722, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI45549022, Length=232, Percent_Identity=25.4310344827586, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI221330053, Length=182, Percent_Identity=28.021978021978, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24586589, Length=264, Percent_Identity=26.1363636363636, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24586599, Length=223, Percent_Identity=25.5605381165919, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24586597, Length=223, Percent_Identity=23.3183856502242, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.135 [H]

Molecular weight: Translated: 69616; Mature: 69616

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKEAIYHRAMSPFVYKYDRETIHIRFHTKRGDIESVDLIWNDPYDWHAEDPAMWNFDPD
CCHHHHHHHHCCCEEEEECCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCCEECCCCCC
KPSYWRTYETPMEKIGFDDAYDYWFIAIKPPFRRLRYGFRLNDGQETAVYTEKGWFEEKP
CCCCCEECCCHHHHCCCCCCCCEEEEEECCCHHHHHCCEEECCCCCEEEEECCCCCCCCC
LDDTGYYFCVPFINPIDIFHAPSWVKDTVWYQIFPDRFANGDASLNPEETLPWNSTEPTT
CCCCCCEEEECCCCCHHHHCCCCHHHHCEEEEECCHHHCCCCCCCCCCCCCCCCCCCCCC
TNFFGGDFQGVIDHIDHLVELGITGIYFCPIFKATTNHKYDTLDYMEIDPQFGTKEKFKE
CCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCEEEEEECCCCCCHHHHHH
MVDLLHENGIKVMLDAVFNHVGYHHPWFQDVVENGPSSEYRDWFYLREFPIATEPKPNYD
HHHHHHHCCCEEHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCC
TFAFEKNMPKINTEHPELKAYLLDVARYWVEEFGIDGWRLDVANEVDHAFWRDFRKAVKA
CEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHC
ANPEAYILGEIWHDAQPWLQGDQFDAVMNYPFTNASLNYFALDRTTASEFRNELTRVEMM
CCCCEEEEEEHHCCCCCCCCCCCCCCEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHH
NTMNVNEVTFNLIDSHDTPRALTRAKGHKGKFKLLMTSMLTYTGSPCIYYGDEIGLEGEQ
HCCCCCCEEEEEECCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
DPGCRRCMPWDEAEEDRNLFRYIQKLIQLRKDHPVLANAGSYRFNLVDDANNAVIIERCT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEEECCCCEEEEEECC
KDETYLVYFNNSDSASKLNPLGYTGAATDLLHDRDLDSLEIELAPFSAAILKLK
CCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCCCEEEEEECCEEEEEEEC
>Mature Secondary Structure
MMKEAIYHRAMSPFVYKYDRETIHIRFHTKRGDIESVDLIWNDPYDWHAEDPAMWNFDPD
CCHHHHHHHHCCCEEEEECCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCCEECCCCCC
KPSYWRTYETPMEKIGFDDAYDYWFIAIKPPFRRLRYGFRLNDGQETAVYTEKGWFEEKP
CCCCCEECCCHHHHCCCCCCCCEEEEEECCCHHHHHCCEEECCCCCEEEEECCCCCCCCC
LDDTGYYFCVPFINPIDIFHAPSWVKDTVWYQIFPDRFANGDASLNPEETLPWNSTEPTT
CCCCCCEEEECCCCCHHHHCCCCHHHHCEEEEECCHHHCCCCCCCCCCCCCCCCCCCCCC
TNFFGGDFQGVIDHIDHLVELGITGIYFCPIFKATTNHKYDTLDYMEIDPQFGTKEKFKE
CCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCEEEEEECCCCCCHHHHHH
MVDLLHENGIKVMLDAVFNHVGYHHPWFQDVVENGPSSEYRDWFYLREFPIATEPKPNYD
HHHHHHHCCCEEHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCC
TFAFEKNMPKINTEHPELKAYLLDVARYWVEEFGIDGWRLDVANEVDHAFWRDFRKAVKA
CEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHC
ANPEAYILGEIWHDAQPWLQGDQFDAVMNYPFTNASLNYFALDRTTASEFRNELTRVEMM
CCCCEEEEEEHHCCCCCCCCCCCCCCEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHH
NTMNVNEVTFNLIDSHDTPRALTRAKGHKGKFKLLMTSMLTYTGSPCIYYGDEIGLEGEQ
HCCCCCCEEEEEECCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
DPGCRRCMPWDEAEEDRNLFRYIQKLIQLRKDHPVLANAGSYRFNLVDDANNAVIIERCT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEEECCCCEEEEEECC
KDETYLVYFNNSDSASKLNPLGYTGAATDLLHDRDLDSLEIELAPFSAAILKLK
CCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCCCEEEEEECCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2482332; 12547200 [H]