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The map label for this gene is proI [H]

Identifier: 229918016

GI number: 229918016

Start: 2250763

End: 2251554

Strand: Reverse

Name: proI [H]

Synonym: EAT1b_2295

Alternate gene names: 229918016

Gene position: 2251554-2250763 (Counterclockwise)

Preceding gene: 229918019

Following gene: 229918010

Centisome position: 75.05

GC content: 54.04

Gene sequence:

>792_bases
ATGATGAATCAACTACTAATGGTCGGTGCAGGATCGATGAGCGAGGCACTCGTTCGCGGGTGGATCGACTCGGGCATCGA
ACCGACACACATCACAATGACAAACCGAAGTGACCGGGAGCGCTTGCTCTTCCTACAAGATGAACTCGGCGTTCAAATTG
TCGAGGACGAAAACGTGAACGACTATGACATCGTCGTATTAGCGATGCAACCAGATGGCGTCATGCCGTATGTAGAATCG
AAAGAATGGAAGACACCGTTACTCGTCTCGGTCGCGGCACATATCGAACCGAGCCAGTTAGAAGAGGCTTCAGGGTTACC
AGCCGTATGTGCCATGCCGAACACGCCTGTCGCCTATCGGAGCGGGATGACGGGTCTATGGTTCGGTCCAGAAACTGATG
AGGCTGATCGCGCAAAAGCGACTTCTCTCTTTGAACGGGTCGGACGAACGGCCGTCACCGATGCAAAGACGATGTCGGCC
TTTATGGCTGCTGCTGGGTGCAGCCCGGCCTTCTTCTATGAAATCGTGGGTGCGATGACGCCTGTCCTAACGGATGTCGG
TTACGATGAACAGACAGCGCGTCAAATTGTCGCTCAAGCGATGAAAGGCTCCGCTGAGTTGTTGTCTCATGAAACACGGA
AGACGAATGAACTGATTGCAGACGTGGCCGCTCCAGGCGGACCGACAGACCGAGGCGTCGGCGTGCTTCGGGAACGGAAC
CTTGCTGACGTGATGGCTTCAGCCTTGCGTGAAAGTGCCAAAGAAGATCATGAAACGATCGAGAAACCATAA

Upstream 100 bases:

>100_bases
TCCATCTCCTTACAAATGAGCAAGATGGGCGATATTCGAGTTCATGTTTGTTATGATAGTCAACATAACTATTGACCGAA
TCGAAAAGAAGAGGTGACGA

Downstream 100 bases:

>100_bases
AAAATGGATGGAAGCGACTCGCTTCCATCCATTTTTTCAATTGATCGTATGTATGATGGACACAATCAAACCCACCACTA
AAATCCCCGGTAATAAATTG

Product: Pyrroline-5-carboxylate reductase

Products: NA

Alternate protein names: P5C reductase 2; P5CR 2 [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MMNQLLMVGAGSMSEALVRGWIDSGIEPTHITMTNRSDRERLLFLQDELGVQIVEDENVNDYDIVVLAMQPDGVMPYVES
KEWKTPLLVSVAAHIEPSQLEEASGLPAVCAMPNTPVAYRSGMTGLWFGPETDEADRAKATSLFERVGRTAVTDAKTMSA
FMAAAGCSPAFFYEIVGAMTPVLTDVGYDEQTARQIVAQAMKGSAELLSHETRKTNELIADVAAPGGPTDRGVGVLRERN
LADVMASALRESAKEDHETIEKP

Sequences:

>Translated_263_residues
MMNQLLMVGAGSMSEALVRGWIDSGIEPTHITMTNRSDRERLLFLQDELGVQIVEDENVNDYDIVVLAMQPDGVMPYVES
KEWKTPLLVSVAAHIEPSQLEEASGLPAVCAMPNTPVAYRSGMTGLWFGPETDEADRAKATSLFERVGRTAVTDAKTMSA
FMAAAGCSPAFFYEIVGAMTPVLTDVGYDEQTARQIVAQAMKGSAELLSHETRKTNELIADVAAPGGPTDRGVGVLRERN
LADVMASALRESAKEDHETIEKP
>Mature_263_residues
MMNQLLMVGAGSMSEALVRGWIDSGIEPTHITMTNRSDRERLLFLQDELGVQIVEDENVNDYDIVVLAMQPDGVMPYVES
KEWKTPLLVSVAAHIEPSQLEEASGLPAVCAMPNTPVAYRSGMTGLWFGPETDEADRAKATSLFERVGRTAVTDAKTMSA
FMAAAGCSPAFFYEIVGAMTPVLTDVGYDEQTARQIVAQAMKGSAELLSHETRKTNELIADVAAPGGPTDRGVGVLRERN
LADVMASALRESAKEDHETIEKP

Specific function: Proline biosynthesis; third (last) step. [C]

COG id: COG0345

COG function: function code E; Pyrroline-5-carboxylate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrroline-5-carboxylate reductase family [H]

Homologues:

Organism=Homo sapiens, GI24797097, Length=264, Percent_Identity=24.6212121212121, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI24797095, Length=264, Percent_Identity=24.6212121212121, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI198041662, Length=265, Percent_Identity=28.6792452830189, Blast_Score=75, Evalue=9e-14,
Organism=Escherichia coli, GI1786585, Length=270, Percent_Identity=27.7777777777778, Blast_Score=82, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17569021, Length=266, Percent_Identity=25.9398496240602, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648116, Length=272, Percent_Identity=26.4705882352941, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR004455
- InterPro:   IPR000304 [H]

Pfam domain/function: PF03807 F420_oxidored [H]

EC number: =1.5.1.2 [H]

Molecular weight: Translated: 28492; Mature: 28492

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNQLLMVGAGSMSEALVRGWIDSGIEPTHITMTNRSDRERLLFLQDELGVQIVEDENVN
CCCCEEEECCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHHEEEEECCCCEEEEECCCCC
DYDIVVLAMQPDGVMPYVESKEWKTPLLVSVAAHIEPSQLEEASGLPAVCAMPNTPVAYR
CEEEEEEEECCCCCCCCCCCCCCCCCCEEHHHHCCCHHHHHHHCCCCEEEECCCCCHHHH
SGMTGLWFGPETDEADRAKATSLFERVGRTAVTDAKTMSAFMAAAGCSPAFFYEIVGAMT
CCCCEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
PVLTDVGYDEQTARQIVAQAMKGSAELLSHETRKTNELIADVAAPGGPTDRGVGVLRERN
HHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCC
LADVMASALRESAKEDHETIEKP
HHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMNQLLMVGAGSMSEALVRGWIDSGIEPTHITMTNRSDRERLLFLQDELGVQIVEDENVN
CCCCEEEECCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHHEEEEECCCCEEEEECCCCC
DYDIVVLAMQPDGVMPYVESKEWKTPLLVSVAAHIEPSQLEEASGLPAVCAMPNTPVAYR
CEEEEEEEECCCCCCCCCCCCCCCCCCEEHHHHCCCHHHHHHHCCCCEEEECCCCCHHHH
SGMTGLWFGPETDEADRAKATSLFERVGRTAVTDAKTMSAFMAAAGCSPAFFYEIVGAMT
CCCCEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
PVLTDVGYDEQTARQIVAQAMKGSAELLSHETRKTNELIADVAAPGGPTDRGVGVLRERN
HHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCC
LADVMASALRESAKEDHETIEKP
HHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 11418582 [H]