Click here to switch to the map view.

The map label for this gene is dppE [H]

Identifier: 229917995

GI number: 229917995

Start: 2228237

End: 2229862

Strand: Reverse

Name: dppE [H]

Synonym: EAT1b_2274

Alternate gene names: 229917995

Gene position: 2229862-2228237 (Counterclockwise)

Preceding gene: 229917997

Following gene: 229917991

Centisome position: 74.33

GC content: 47.29

Gene sequence:

>1626_bases
ATGCGTAACAAAAAATGGCTAGCGTTATCGGGGGTCGCATTATTGGCGACGGCAGCTTGTAGCACAGGGGATGACTCATC
GAGTTCAGAAGGTTCATCTTCGAGCGATGCGAAAAAAGAAATCACAATGGTATCGGCGACGGACTTGCCGCAGCTCGATC
CGACACTTACAACAGACTCGACATCGATCATCGTGACAAACAACGTGTTCGAAGGATTGTATCGTCTAGACGAGAACAAC
CAACCGACACCGGGGATTGCCGAAGATGTTGAAGTATCTGAGGATGGCCTTACATACACGTTCAAGTTACGTGACGCAAA
CTGGTCAGACGGTTCTCCAATTACAGCTGAAGACTTCGTTTACTCTTGGAAACGTGCCCTCAATCCTGAAACAGGTGCGG
AGTATGCCTACATTCTTCAAGACTTAAAAAACGCCAACAAAATCCTTGCAGGTGAAATGTCGCTCGACGACCTCGGCGCA
AAAGCGATTGACGAGAAAACGCTTGAAGTACAATTGGAAGCACCGGCTCCTTATTTCTTAGGATTGACTGGTTTCCCGAC
GTATATGCCGCAAAAACAAGAGTACGTCGAAGAGCAAGGCGAAGAATTCGCAACATCTGTCGACAAGACACTCTATAACG
GTCCTTACGTCTTGAGCGAGTGGCAAGACAATGCAGGATGGGTTTACAAGAAGAACCCTGAATATTGGGATGCTGAAAAC
GTCAAGATGGACACGATCAACGTCAAAGTCGTAAAAGACGTCTCGACTGGGGTCAACTTGTTCGAGTCGGGTGAAGCAGA
CTATACGCTCCTCTCGTCTGAATTCGTTCCGCAATTCGAAGATAGCGACGAGTTCAAAACGCGTGCCGATGCTCGAATCA
ACTTCCTTCGTTTCAACCAAAAGAACGAAGCGCTCCAAAACGTCAACATCCGTGAAGCACTTGCGAAAGGATTCGACAAA
CAGAGTGTGACAGATGTCATCTTAACAGATGGGTCGCAACCTGCGAACTTTATCGTAGCGAAAGACTTCACGTTCACAGA
AGACGGGGCTGACTTCCGTGAGAAGTATCCAGACCTCCTCAACTACAACGTGGATGAAGCAAAAGCCGCGTGGGAAAAAG
GATTAGAAGAACTAGGTGTCGAAACGATCGAACTCGAGTTCCTCTCACGTGACGAGGAAGCGTTCAAGAAAGTGAACGAG
TTCATCAAAGGTGAACTTGAGAAGAACTTGCCAGGTTTGACACTTAACATCAAACAGCAACCGTTCAAAAACTTCCTTGA
CCTTGAAGGAAAAGGTGAGTACGACGTTTCTGCAGCTGGTTGGGGTCCTGACTATCAAGATCCAATGACATACCTCGACA
TGTGGGTCACAGACGGACCATTCAACCGCATGGAGTACTCGAATGACGAGTATGATCAGTTGATTCAATCTGCGAAGAAA
GAAGCCGATCAGATGAAACGTTGGGAAACGATGCAAGAAGCAGAGCGCATCCTTCTCGAAGAAGACTTTGCCATCGCTCC
GATTTATCAAAAAGGTGAGGCATACTTAGAGCGTTCAAACATTGAGAACATGTACCGTCACCCGTTCGGTGCCGATGCAA
GCTTCAAATGGCTTGACGTCAAATAA

Upstream 100 bases:

>100_bases
ATATTTTCGGATAATTTGAAAAATTCCATTGCATATTCATGTCAGATGTAGTTTAATTACAACATATTCTAAATTATCAG
AATTTTCGGGAGGTTGTATA

Downstream 100 bases:

>100_bases
TAAGTATAACCTCCTTGATTCTTACATGGATCAAGGAGGTTTTCTGTCGTGAAAGGGGCTTCTATCTAATGCAGAGTTGG
ACACTATGATTCACCTTATT

Product: extracellular solute-binding protein family 5

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MRNKKWLALSGVALLATAACSTGDDSSSSEGSSSSDAKKEITMVSATDLPQLDPTLTTDSTSIIVTNNVFEGLYRLDENN
QPTPGIAEDVEVSEDGLTYTFKLRDANWSDGSPITAEDFVYSWKRALNPETGAEYAYILQDLKNANKILAGEMSLDDLGA
KAIDEKTLEVQLEAPAPYFLGLTGFPTYMPQKQEYVEEQGEEFATSVDKTLYNGPYVLSEWQDNAGWVYKKNPEYWDAEN
VKMDTINVKVVKDVSTGVNLFESGEADYTLLSSEFVPQFEDSDEFKTRADARINFLRFNQKNEALQNVNIREALAKGFDK
QSVTDVILTDGSQPANFIVAKDFTFTEDGADFREKYPDLLNYNVDEAKAAWEKGLEELGVETIELEFLSRDEEAFKKVNE
FIKGELEKNLPGLTLNIKQQPFKNFLDLEGKGEYDVSAAGWGPDYQDPMTYLDMWVTDGPFNRMEYSNDEYDQLIQSAKK
EADQMKRWETMQEAERILLEEDFAIAPIYQKGEAYLERSNIENMYRHPFGADASFKWLDVK

Sequences:

>Translated_541_residues
MRNKKWLALSGVALLATAACSTGDDSSSSEGSSSSDAKKEITMVSATDLPQLDPTLTTDSTSIIVTNNVFEGLYRLDENN
QPTPGIAEDVEVSEDGLTYTFKLRDANWSDGSPITAEDFVYSWKRALNPETGAEYAYILQDLKNANKILAGEMSLDDLGA
KAIDEKTLEVQLEAPAPYFLGLTGFPTYMPQKQEYVEEQGEEFATSVDKTLYNGPYVLSEWQDNAGWVYKKNPEYWDAEN
VKMDTINVKVVKDVSTGVNLFESGEADYTLLSSEFVPQFEDSDEFKTRADARINFLRFNQKNEALQNVNIREALAKGFDK
QSVTDVILTDGSQPANFIVAKDFTFTEDGADFREKYPDLLNYNVDEAKAAWEKGLEELGVETIELEFLSRDEEAFKKVNE
FIKGELEKNLPGLTLNIKQQPFKNFLDLEGKGEYDVSAAGWGPDYQDPMTYLDMWVTDGPFNRMEYSNDEYDQLIQSAKK
EADQMKRWETMQEAERILLEEDFAIAPIYQKGEAYLERSNIENMYRHPFGADASFKWLDVK
>Mature_541_residues
MRNKKWLALSGVALLATAACSTGDDSSSSEGSSSSDAKKEITMVSATDLPQLDPTLTTDSTSIIVTNNVFEGLYRLDENN
QPTPGIAEDVEVSEDGLTYTFKLRDANWSDGSPITAEDFVYSWKRALNPETGAEYAYILQDLKNANKILAGEMSLDDLGA
KAIDEKTLEVQLEAPAPYFLGLTGFPTYMPQKQEYVEEQGEEFATSVDKTLYNGPYVLSEWQDNAGWVYKKNPEYWDAEN
VKMDTINVKVVKDVSTGVNLFESGEADYTLLSSEFVPQFEDSDEFKTRADARINFLRFNQKNEALQNVNIREALAKGFDK
QSVTDVILTDGSQPANFIVAKDFTFTEDGADFREKYPDLLNYNVDEAKAAWEKGLEELGVETIELEFLSRDEEAFKKVNE
FIKGELEKNLPGLTLNIKQQPFKNFLDLEGKGEYDVSAAGWGPDYQDPMTYLDMWVTDGPFNRMEYSNDEYDQLIQSAKK
EADQMKRWETMQEAERILLEEDFAIAPIYQKGEAYLERSNIENMYRHPFGADASFKWLDVK

Specific function: Part of the binding-protein-dependent transport system for dipeptides; probably responsible for the binding of dipeptides with high affinity. Is expressed to facilitate adaptation to nutrient deficiency conditions, which also induce sporulation [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=521, Percent_Identity=29.1746641074856, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1789397, Length=516, Percent_Identity=28.1007751937984, Blast_Score=206, Evalue=4e-54,
Organism=Escherichia coli, GI87081878, Length=507, Percent_Identity=28.7968441814596, Blast_Score=191, Evalue=9e-50,
Organism=Escherichia coli, GI1787762, Length=502, Percent_Identity=24.7011952191235, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI1787052, Length=499, Percent_Identity=22.4448897795591, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1789887, Length=418, Percent_Identity=23.6842105263158, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61191; Mature: 61191

Theoretical pI: Translated: 4.07; Mature: 4.07

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNKKWLALSGVALLATAACSTGDDSSSSEGSSSSDAKKEITMVSATDLPQLDPTLTTDS
CCCCCEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCHHEEEEEEECCCCCCCCCEECCC
TSIIVTNNVFEGLYRLDENNQPTPGIAEDVEVSEDGLTYTFKLRDANWSDGSPITAEDFV
CEEEEECHHHHHHHHHCCCCCCCCCCCCCCEECCCCCEEEEEEECCCCCCCCCCCHHHHH
YSWKRALNPETGAEYAYILQDLKNANKILAGEMSLDDLGAKAIDEKTLEVQLEAPAPYFL
HHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCCCHHHHCCHHHCCCEEEEEEECCCCEEE
GLTGFPTYMPQKQEYVEEQGEEFATSVDKTLYNGPYVLSEWQDNAGWVYKKNPEYWDAEN
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEECCCCCCCCCCC
VKMDTINVKVVKDVSTGVNLFESGEADYTLLSSEFVPQFEDSDEFKTRADARINFLRFNQ
EEEEEEEEEEEEECCCCCCHHCCCCCCEEEECCCCCCCCCCCHHHHHHHCCEEEEEEECC
KNEALQNVNIREALAKGFDKQSVTDVILTDGSQPANFIVAKDFTFTEDGADFREKYPDLL
HHHHHHCCCHHHHHHCCCCCCCCCEEEEECCCCCCCEEEEECCEECCCCCHHHHHCHHHH
NYNVDEAKAAWEKGLEELGVETIELEFLSRDEEAFKKVNEFIKGELEKNLPGLTLNIKQQ
CCCHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCC
PFKNFLDLEGKGEYDVSAAGWGPDYQDPMTYLDMWVTDGPFNRMEYSNDEYDQLIQSAKK
HHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHEECCCCCCCCCCCCCHHHHHHHHHHH
EADQMKRWETMQEAERILLEEDFAIAPIYQKGEAYLERSNIENMYRHPFGADASFKWLDV
HHHHHHHHHHHHHHHHHHHHCCCEEECCHHCCHHHHHHHCHHHHHCCCCCCCCCEEEEEC
K
C
>Mature Secondary Structure
MRNKKWLALSGVALLATAACSTGDDSSSSEGSSSSDAKKEITMVSATDLPQLDPTLTTDS
CCCCCEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCHHEEEEEEECCCCCCCCCEECCC
TSIIVTNNVFEGLYRLDENNQPTPGIAEDVEVSEDGLTYTFKLRDANWSDGSPITAEDFV
CEEEEECHHHHHHHHHCCCCCCCCCCCCCCEECCCCCEEEEEEECCCCCCCCCCCHHHHH
YSWKRALNPETGAEYAYILQDLKNANKILAGEMSLDDLGAKAIDEKTLEVQLEAPAPYFL
HHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCCCHHHHCCHHHCCCEEEEEEECCCCEEE
GLTGFPTYMPQKQEYVEEQGEEFATSVDKTLYNGPYVLSEWQDNAGWVYKKNPEYWDAEN
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEECCCCCCCCCCC
VKMDTINVKVVKDVSTGVNLFESGEADYTLLSSEFVPQFEDSDEFKTRADARINFLRFNQ
EEEEEEEEEEEEECCCCCCHHCCCCCCEEEECCCCCCCCCCCHHHHHHHCCEEEEEEECC
KNEALQNVNIREALAKGFDKQSVTDVILTDGSQPANFIVAKDFTFTEDGADFREKYPDLL
HHHHHHCCCHHHHHHCCCCCCCCCEEEEECCCCCCCEEEEECCEECCCCCHHHHHCHHHH
NYNVDEAKAAWEKGLEELGVETIELEFLSRDEEAFKKVNEFIKGELEKNLPGLTLNIKQQ
CCCHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCC
PFKNFLDLEGKGEYDVSAAGWGPDYQDPMTYLDMWVTDGPFNRMEYSNDEYDQLIQSAKK
HHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHEECCCCCCCCCCCCCHHHHHHHHHHH
EADQMKRWETMQEAERILLEEDFAIAPIYQKGEAYLERSNIENMYRHPFGADASFKWLDV
HHHHHHHHHHHHHHHHHHHHCCCEEECCHHCCHHHHHHHCHHHHHCCCCCCCCCEEEEEC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1766370; 9384377 [H]