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The map label for this gene is yrvJ [H]

Identifier: 229917929

GI number: 229917929

Start: 2142967

End: 2144469

Strand: Reverse

Name: yrvJ [H]

Synonym: EAT1b_2208

Alternate gene names: 229917929

Gene position: 2144469-2142967 (Counterclockwise)

Preceding gene: 229917930

Following gene: 229917926

Centisome position: 71.48

GC content: 47.97

Gene sequence:

>1503_bases
TTGAATGAAACAAAACCGTCATGGAGTTTAAAGCTGTTACTTAGTTCTTCACTTGCAATATCCGGGGCAGTAGTCGTCCC
GTTCCAATTGGATTCATCTGGTATTCAATCGACTGTCGCCGAAGCCGCCTCGATCTATACCACTACCGCCAACTTAAATC
TTCGCCTCAGCGCAGCCACTTGGAGCCCTGTCCTCTTGACCATCCCATCTGGCTCAAGAGTCACGTATATTAGTACATAC
GGTTCGTGGTACAAGGTCAGTTATGGAGGCAAAACAGGCTATGTCGCTTCTCAATATGTTTCGGTCTCAAACACTTCTGC
TTACTATAAGACAACAGACCGATTAAATATGCGCCTCACCGCTGCTTCATGGAGTGATGTGGTCACCGTGATCCCTGCCG
ACGCGACCGTGAAGTATGTCAGTCGTTATGGGTCATGGTACAAAGTGACGTTCAATGGAAAGACGGGCTATGTCGCTTCT
GCTTATTTAACACCTACGAGTGCCCCAGTCCCACCTTCGGACTACTATAAGACGACTGCCAACCTGAACCTGCGTCTCAG
CGCCGCTTCATGGAGTTCCGTCGTCACGACGATTCCATCGGGTGCGACTGTCAAATACGTGAGCCGTTACGGATCATGGT
ATAAGGTCACCTATAATGGGAAAACCGGTTACGTTTCTTCTGACTACTTAACGGCGACGACAGCTCCCGTAACCCCGTCT
TCTTATTATGAAACGACCGTAAACTTGAATATGCGTCTTAGCGCCGCTTCATGGAGCGATGTCTTGACCGTCATTCCAGC
TGGATCAGTCGTCAAATATGTCAGCCGTTATGACTCGTGGTATAAAGTCACATACAACGGCAAAACCGGGTATGTAGCAT
CCGAGTACTTGAAACCTACGACTGTTACGACACCAACGGATACCAACGGAACCGGTAAAACGGTCGTCATCGACGCAGGA
CATGGTGGAAACGACCCTGGCGCTGTCTATGGCTCTGTTTATGAGAAAGTGATCGCGCTTGATATCGCGAAACGTCTAGC
TGGCATCTTATCGAGCACCTATGACTACAACGTTAAATTGACTCGCTCAAACGATACATACCTCTCTCTTGAACAGCGTG
TGTCACTCAACAAGTCTTATAAAGGGGATGTGTTCGTCAGTCTTCATGCGAACTCTTCCGTTTATAACACGGCACATGGA
CACGAGGTACTCGTCCCGACGTCAGAAAGTTATACGACAAACCCTTACGTGTCCGCCAGCCGTAGTCTCGGCTCAAGTAT
CAACAAAGAGATTGCTTCACGGATTTCTACGATTCAAAACCGTGGGGTCAAATATCAGAACGTTTACGTGGTTGGACGAA
ACGTCTCGCCTTCTACCCTTGTCGAATACGGATTCATCAGTAATTCAAGCGACCGTTCTTACTTGACGAATACGACATAT
CGCCAACGAATGGCTGAAGCAACAGCATCCGGCATTCATCAATTCATGCGATCTTATTACTAA

Upstream 100 bases:

>100_bases
CCATTTTTTCCTTTTTACTTATATAATAAACAGGGACAACCTAGATGCACTCTCATACACCGCCCTTGTCACCAGTTTAA
TGAGTAAAGGAGTTTGAACA

Downstream 100 bases:

>100_bases
AAAAAGGAACTTAGCACTCAGCTAAGTTCCTTTTTTGATATGGACTCGCCAAATCATATGGGATGCCCCAGGTCCCCAAT
AATGTTCGAGGATATGAGAC

Product: cell wall hydrolase/autolysin

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MNETKPSWSLKLLLSSSLAISGAVVVPFQLDSSGIQSTVAEAASIYTTTANLNLRLSAATWSPVLLTIPSGSRVTYISTY
GSWYKVSYGGKTGYVASQYVSVSNTSAYYKTTDRLNMRLTAASWSDVVTVIPADATVKYVSRYGSWYKVTFNGKTGYVAS
AYLTPTSAPVPPSDYYKTTANLNLRLSAASWSSVVTTIPSGATVKYVSRYGSWYKVTYNGKTGYVSSDYLTATTAPVTPS
SYYETTVNLNMRLSAASWSDVLTVIPAGSVVKYVSRYDSWYKVTYNGKTGYVASEYLKPTTVTTPTDTNGTGKTVVIDAG
HGGNDPGAVYGSVYEKVIALDIAKRLAGILSSTYDYNVKLTRSNDTYLSLEQRVSLNKSYKGDVFVSLHANSSVYNTAHG
HEVLVPTSESYTTNPYVSASRSLGSSINKEIASRISTIQNRGVKYQNVYVVGRNVSPSTLVEYGFISNSSDRSYLTNTTY
RQRMAEATASGIHQFMRSYY

Sequences:

>Translated_500_residues
MNETKPSWSLKLLLSSSLAISGAVVVPFQLDSSGIQSTVAEAASIYTTTANLNLRLSAATWSPVLLTIPSGSRVTYISTY
GSWYKVSYGGKTGYVASQYVSVSNTSAYYKTTDRLNMRLTAASWSDVVTVIPADATVKYVSRYGSWYKVTFNGKTGYVAS
AYLTPTSAPVPPSDYYKTTANLNLRLSAASWSSVVTTIPSGATVKYVSRYGSWYKVTYNGKTGYVSSDYLTATTAPVTPS
SYYETTVNLNMRLSAASWSDVLTVIPAGSVVKYVSRYDSWYKVTYNGKTGYVASEYLKPTTVTTPTDTNGTGKTVVIDAG
HGGNDPGAVYGSVYEKVIALDIAKRLAGILSSTYDYNVKLTRSNDTYLSLEQRVSLNKSYKGDVFVSLHANSSVYNTAHG
HEVLVPTSESYTTNPYVSASRSLGSSINKEIASRISTIQNRGVKYQNVYVVGRNVSPSTLVEYGFISNSSDRSYLTNTTY
RQRMAEATASGIHQFMRSYY
>Mature_500_residues
MNETKPSWSLKLLLSSSLAISGAVVVPFQLDSSGIQSTVAEAASIYTTTANLNLRLSAATWSPVLLTIPSGSRVTYISTY
GSWYKVSYGGKTGYVASQYVSVSNTSAYYKTTDRLNMRLTAASWSDVVTVIPADATVKYVSRYGSWYKVTFNGKTGYVAS
AYLTPTSAPVPPSDYYKTTANLNLRLSAASWSSVVTTIPSGATVKYVSRYGSWYKVTYNGKTGYVSSDYLTATTAPVTPS
SYYETTVNLNMRLSAASWSDVLTVIPAGSVVKYVSRYDSWYKVTYNGKTGYVASEYLKPTTVTTPTDTNGTGKTVVIDAG
HGGNDPGAVYGSVYEKVIALDIAKRLAGILSSTYDYNVKLTRSNDTYLSLEQRVSLNKSYKGDVFVSLHANSSVYNTAHG
HEVLVPTSESYTTNPYVSASRSLGSSINKEIASRISTIQNRGVKYQNVYVVGRNVSPSTLVEYGFISNSSDRSYLTNTTY
RQRMAEATASGIHQFMRSYY

Specific function: Probably involved in cell-wall metabolism [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1790611, Length=265, Percent_Identity=28.3018867924528, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR017293
- InterPro:   IPR003646
- InterPro:   IPR013247 [H]

Pfam domain/function: PF01520 Amidase_3; PF08239 SH3_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 54432; Mature: 54432

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNETKPSWSLKLLLSSSLAISGAVVVPFQLDSSGIQSTVAEAASIYTTTANLNLRLSAAT
CCCCCCCEEEEEEEECCEEECCEEEEEEEECCCHHHHHHHHHHEEEEEEEEEEEEEEEEC
WSPVLLTIPSGSRVTYISTYGSWYKVSYGGKTGYVASQYVSVSNTSAYYKTTDRLNMRLT
CCCEEEEECCCCEEEEEEECCCEEEEEECCCCCCEEEEEEEECCCCEEEEECCCEEEEEE
AASWSDVVTVIPADATVKYVSRYGSWYKVTFNGKTGYVASAYLTPTSAPVPPSDYYKTTA
ECCCCCEEEEEECCHHHHHHHHCCCEEEEEECCCCCEEEEEEECCCCCCCCCCHHEEEEE
NLNLRLSAASWSSVVTTIPSGATVKYVSRYGSWYKVTYNGKTGYVSSDYLTATTAPVTPS
CEEEEEECCCCCEEEEECCCCCEEHHHHHCCCEEEEEECCCCCEEECCEEEEECCCCCCC
SYYETTVNLNMRLSAASWSDVLTVIPAGSVVKYVSRYDSWYKVTYNGKTGYVASEYLKPT
CCEEEEEEEEEEEECCCCCCEEEEECCCHHHHHHHHCCCEEEEEECCCCCCEEHHHCCCC
TVTTPTDTNGTGKTVVIDAGHGGNDPGAVYGSVYEKVIALDIAKRLAGILSSTYDYNVKL
EEECCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
TRSNDTYLSLEQRVSLNKSYKGDVFVSLHANSSVYNTAHGHEVLVPTSESYTTNPYVSAS
EECCCCEEEEEHHHCCCCCCCCCEEEEEECCCCEEECCCCCEEEEECCCCCCCCCCCCCH
RSLGSSINKEIASRISTIQNRGVKYQNVYVVGRNVSPSTLVEYGFISNSSDRSYLTNTTY
HHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHEEEEEECCCCCCCCEECCCHH
RQRMAEATASGIHQFMRSYY
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNETKPSWSLKLLLSSSLAISGAVVVPFQLDSSGIQSTVAEAASIYTTTANLNLRLSAAT
CCCCCCCEEEEEEEECCEEECCEEEEEEEECCCHHHHHHHHHHEEEEEEEEEEEEEEEEC
WSPVLLTIPSGSRVTYISTYGSWYKVSYGGKTGYVASQYVSVSNTSAYYKTTDRLNMRLT
CCCEEEEECCCCEEEEEEECCCEEEEEECCCCCCEEEEEEEECCCCEEEEECCCEEEEEE
AASWSDVVTVIPADATVKYVSRYGSWYKVTFNGKTGYVASAYLTPTSAPVPPSDYYKTTA
ECCCCCEEEEEECCHHHHHHHHCCCEEEEEECCCCCEEEEEEECCCCCCCCCCHHEEEEE
NLNLRLSAASWSSVVTTIPSGATVKYVSRYGSWYKVTYNGKTGYVSSDYLTATTAPVTPS
CEEEEEECCCCCEEEEECCCCCEEHHHHHCCCEEEEEECCCCCEEECCEEEEECCCCCCC
SYYETTVNLNMRLSAASWSDVLTVIPAGSVVKYVSRYDSWYKVTYNGKTGYVASEYLKPT
CCEEEEEEEEEEEECCCCCCEEEEECCCHHHHHHHHCCCEEEEEECCCCCCEEHHHCCCC
TVTTPTDTNGTGKTVVIDAGHGGNDPGAVYGSVYEKVIALDIAKRLAGILSSTYDYNVKL
EEECCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
TRSNDTYLSLEQRVSLNKSYKGDVFVSLHANSSVYNTAHGHEVLVPTSESYTTNPYVSAS
EECCCCEEEEEHHHCCCCCCCCCEEEEEECCCCEEECCCCCEEEEECCCCCCCCCCCCCH
RSLGSSINKEIASRISTIQNRGVKYQNVYVVGRNVSPSTLVEYGFISNSSDRSYLTNTTY
HHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHEEEEEECCCCCCCCEECCCHH
RQRMAEATASGIHQFMRSYY
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]