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The map label for this gene is queD [H]

Identifier: 229917715

GI number: 229917715

Start: 1947888

End: 1948346

Strand: Reverse

Name: queD [H]

Synonym: EAT1b_1992

Alternate gene names: 229917715

Gene position: 1948346-1947888 (Counterclockwise)

Preceding gene: 229917716

Following gene: 229917714

Centisome position: 64.95

GC content: 47.93

Gene sequence:

>459_bases
ATGAAACATGCTCCATTCCTCGCTCCGACCAGTGTTGAATCAAAGCAAGAAGGCTCGCTCATCTACTGTCCGCATCGCGT
TCAAATCGTCAAAGAAGTGACGTTCGACGCAGCGCACCATCTCTTTGATTATGACGGCAAATGTCGTGCTCTTCATGGTC
ATACGTATAAGCTCCAAATGGGTGTCAGCGGCTTTCTCGACAATCGCGGGATGACGCTCGACTTTGGTGACTTGAAAAAG
ATCTTCAAAGAAGAGCTTGAGCCATATCTCGACCATCGCTATTTGAACGAGTCACTCCCTTATATGAATACAACTGCAGA
AAACATGTGCTATTGGATTTTTGAACAACTAGCGACACACTTGCCAAATGAACGCGACGTCCGTGTCGAATTCGTCCGCT
TGTATGAAACGCCGACGTCGTACGCTGAGTTCCGCCGTGAATGGTTGGTGGAATCGTGA

Upstream 100 bases:

>100_bases
CGCTCACTTGCTACAACGGTGTCATCGGTTCAGGTTGTGGCGAATGTCCGGCGTGTAAGCTTCGTCAAAACGGACTCACA
GCTTACGAGGAGGTGCGCGT

Downstream 100 bases:

>100_bases
AAATTCCTGTTCTTGAAGTGTTCGGACCAACCTTCCAAGGCGAAGGCCGGGCCATCGGACAGAAGACGATGTTCGTCCGG
ACCGCCGGTTGCGATTATCG

Product: 6-pyruvoyl tetrahydropterin synthase and hypothetical protein

Products: 2-Amino- 4-Oxo-6-(1',2'-Dioxoprolyl)-7,8-Dihydroxypteridine; Triphosphate. [C]

Alternate protein names: CPH4 synthase; Queuosine biosynthesis protein queD [H]

Number of amino acids: Translated: 152; Mature: 152

Protein sequence:

>152_residues
MKHAPFLAPTSVESKQEGSLIYCPHRVQIVKEVTFDAAHHLFDYDGKCRALHGHTYKLQMGVSGFLDNRGMTLDFGDLKK
IFKEELEPYLDHRYLNESLPYMNTTAENMCYWIFEQLATHLPNERDVRVEFVRLYETPTSYAEFRREWLVES

Sequences:

>Translated_152_residues
MKHAPFLAPTSVESKQEGSLIYCPHRVQIVKEVTFDAAHHLFDYDGKCRALHGHTYKLQMGVSGFLDNRGMTLDFGDLKK
IFKEELEPYLDHRYLNESLPYMNTTAENMCYWIFEQLATHLPNERDVRVEFVRLYETPTSYAEFRREWLVES
>Mature_152_residues
MKHAPFLAPTSVESKQEGSLIYCPHRVQIVKEVTFDAAHHLFDYDGKCRALHGHTYKLQMGVSGFLDNRGMTLDFGDLKK
IFKEELEPYLDHRYLNESLPYMNTTAENMCYWIFEQLATHLPNERDVRVEFVRLYETPTSYAEFRREWLVES

Specific function: Catalyzes the conversion of 7,8-dihydroneopterin triphosphate (H2NTP) to 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) and acetaldehyde [H]

COG id: COG0720

COG function: function code H; 6-pyruvoyl-tetrahydropterin synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTPS family. QueD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789124, Length=122, Percent_Identity=35.2459016393443, Blast_Score=64, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007116
- InterPro:   IPR007115 [H]

Pfam domain/function: PF01242 PTPS [H]

EC number: 4.2.3.12 [C]

Molecular weight: Translated: 17941; Mature: 17941

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHAPFLAPTSVESKQEGSLIYCPHRVQIVKEVTFDAAHHLFDYDGKCRALHGHTYKLQM
CCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEEEE
GVSGFLDNRGMTLDFGDLKKIFKEELEPYLDHRYLNESLPYMNTTAENMCYWIFEQLATH
CCCHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
LPNERDVRVEFVRLYETPTSYAEFRREWLVES
CCCCCCCEEEEEEEECCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MKHAPFLAPTSVESKQEGSLIYCPHRVQIVKEVTFDAAHHLFDYDGKCRALHGHTYKLQM
CCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEEEE
GVSGFLDNRGMTLDFGDLKKIFKEELEPYLDHRYLNESLPYMNTTAENMCYWIFEQLATH
CCCHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
LPNERDVRVEFVRLYETPTSYAEFRREWLVES
CCCCCCCEEEEEEEECCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 17.7 {6-(L-erythro-1,2-dihydroxypropyl} 5 {6-(L-erythro-1,2-dihydroxypropyl} 0.0091 {6-(L-erythro-1,2-dihydroxypropyl} 0.008 {6-(L-erythro-1,2-dihydroxypropyl} [C]

Substrates: 2-Amino-4-Oxo-6-(Erythro-1',2',3'- Trihydroxyprolyl)-7,8-Dihydroxypteridine Triphosphate [C]

Specific reaction: 2-Amino-4-Oxo-6-(Erythro-1',2',3'- Trihydroxyprolyl)-7,8-Dihydroxypteridine Triphosphate = 2-Amino- 4-Oxo-6-(1',2'-Dioxoprolyl)-7,8-Dihydroxypteridine + Triphosphate. [C]

General reaction: Intramolecular redox reaction; P-O bond cleavage; Triphosphate elimination [C]

Inhibitor: (NH4)2SO4 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]