Click here to switch to the map view.

The map label for this gene is queE [H]

Identifier: 229917714

GI number: 229917714

Start: 1947196

End: 1947906

Strand: Reverse

Name: queE [H]

Synonym: EAT1b_1991

Alternate gene names: 229917714

Gene position: 1947906-1947196 (Counterclockwise)

Preceding gene: 229917715

Following gene: 229917713

Centisome position: 64.93

GC content: 55.13

Gene sequence:

>711_bases
ATGGTTGGTGGAATCGTGAAAATTCCTGTTCTTGAAGTGTTCGGACCAACCTTCCAAGGCGAAGGCCGGGCCATCGGACA
GAAGACGATGTTCGTCCGGACCGCCGGTTGCGATTATCGCTGTTCGTGGTGCGATTCCGCCTTCACGTGGGACGGTTCAG
AAAAACCAGACATGCTGACAGCCGATGAGGTCATCGCTCGCCTGGACGCACTGGGTATGTACGATTACGTCACCATCTCG
GGTGGCAATCCGCTCCTCATCGCGGCTATGGAAGACCTCGTTGTGAAGTTGAAAAAACGTGGTGTCACACTCGCTGTCGA
GACGCAAGGTAGCCGCTATCAAGATTGGCTCACACAAATCGATGACGTGACCCTCAGTCCGAAACCTCCATCGTCTGGTA
TGAAGACGAACTGGGAGCAACTCGACGCCATCGTCGAACGGTTACGCCCGGAGCAGACGACATTCAAAGTCGCTGTCTTT
GATGAAGCGGACCTCGCCTACGCAAAAGAAGTGCAGCAACGCTACTCACCGGACGTCATGTATTTGTCGGCCGGTAACCC
AGAGCCTGGTGCAGACGGAGACATCACCGATGCCCAGCTCCGTCGTTTGAAACAACTATGGGAAGACGTCGCACGCGACC
CGTCATGGCAAAGCGTCCGCGTCCTACCACAACTACACACATTACTGTACGCCAACGAACGTGGCGTCTAA

Upstream 100 bases:

>100_bases
TTGAACAACTAGCGACACACTTGCCAAATGAACGCGACGTCCGTGTCGAATTCGTCCGCTTGTATGAAACGCCGACGTCG
TACGCTGAGTTCCGCCGTGA

Downstream 100 bases:

>100_bases
GGAGAATGAACATGAGACCAGAAGACTTACAAGACCTCTCGCTCCTCGGACAGAAGAGCGTCCCATATATTTTTGAATAC
ACACCTGACGTCCTTGAAGC

Product: 7-cyano-7-deazaguanosine (preQ0) biosynthesis protein QueE

Products: NA

Alternate protein names: CDG synthase; Queuosine biosynthesis protein queE [H]

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MVGGIVKIPVLEVFGPTFQGEGRAIGQKTMFVRTAGCDYRCSWCDSAFTWDGSEKPDMLTADEVIARLDALGMYDYVTIS
GGNPLLIAAMEDLVVKLKKRGVTLAVETQGSRYQDWLTQIDDVTLSPKPPSSGMKTNWEQLDAIVERLRPEQTTFKVAVF
DEADLAYAKEVQQRYSPDVMYLSAGNPEPGADGDITDAQLRRLKQLWEDVARDPSWQSVRVLPQLHTLLYANERGV

Sequences:

>Translated_236_residues
MVGGIVKIPVLEVFGPTFQGEGRAIGQKTMFVRTAGCDYRCSWCDSAFTWDGSEKPDMLTADEVIARLDALGMYDYVTIS
GGNPLLIAAMEDLVVKLKKRGVTLAVETQGSRYQDWLTQIDDVTLSPKPPSSGMKTNWEQLDAIVERLRPEQTTFKVAVF
DEADLAYAKEVQQRYSPDVMYLSAGNPEPGADGDITDAQLRRLKQLWEDVARDPSWQSVRVLPQLHTLLYANERGV
>Mature_236_residues
MVGGIVKIPVLEVFGPTFQGEGRAIGQKTMFVRTAGCDYRCSWCDSAFTWDGSEKPDMLTADEVIARLDALGMYDYVTIS
GGNPLLIAAMEDLVVKLKKRGVTLAVETQGSRYQDWLTQIDDVTLSPKPPSSGMKTNWEQLDAIVERLRPEQTTFKVAVF
DEADLAYAKEVQQRYSPDVMYLSAGNPEPGADGDITDAQLRRLKQLWEDVARDPSWQSVRVLPQLHTLLYANERGV

Specific function: Catalyzes the conversion of 6-carboxy-5,6,7,8- tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG) [H]

COG id: COG0602

COG function: function code O; Organic radical activating enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. 7-carboxy-7- deazaguanine synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017742
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 26308; Mature: 26308

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGGIVKIPVLEVFGPTFQGEGRAIGQKTMFVRTAGCDYRCSWCDSAFTWDGSEKPDMLT
CCCCEEECCHHHHCCCCCCCCCCEECCEEEEEEECCCCEEEECCCCCEECCCCCCCCCCC
ADEVIARLDALGMYDYVTISGGNPLLIAAMEDLVVKLKKRGVTLAVETQGSRYQDWLTQI
HHHHHHHHHHCCCEEEEEECCCCEEEEEHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHH
DDVTLSPKPPSSGMKTNWEQLDAIVERLRPEQTTFKVAVFDEADLAYAKEVQQRYSPDVM
CCEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHCCCCEE
YLSAGNPEPGADGDITDAQLRRLKQLWEDVARDPSWQSVRVLPQLHTLLYANERGV
EEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MVGGIVKIPVLEVFGPTFQGEGRAIGQKTMFVRTAGCDYRCSWCDSAFTWDGSEKPDMLT
CCCCEEECCHHHHCCCCCCCCCCEECCEEEEEEECCCCEEEECCCCCEECCCCCCCCCCC
ADEVIARLDALGMYDYVTISGGNPLLIAAMEDLVVKLKKRGVTLAVETQGSRYQDWLTQI
HHHHHHHHHHCCCEEEEEECCCCEEEEEHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHH
DDVTLSPKPPSSGMKTNWEQLDAIVERLRPEQTTFKVAVFDEADLAYAKEVQQRYSPDVM
CCEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHCCCCEE
YLSAGNPEPGADGDITDAQLRRLKQLWEDVARDPSWQSVRVLPQLHTLLYANERGV
EEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]