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The map label for this gene is pepT

Identifier: 229917651

GI number: 229917651

Start: 1887131

End: 1888369

Strand: Reverse

Name: pepT

Synonym: EAT1b_1928

Alternate gene names: 229917651

Gene position: 1888369-1887131 (Counterclockwise)

Preceding gene: 229917652

Following gene: 229917650

Centisome position: 62.95

GC content: 50.77

Gene sequence:

>1239_bases
ATGCAAGCAAAATTAATTGAACGCTTAATGACGTACGCGAAGATTGATACACAGTCTGACTTCACGAGTACGACAACACC
ATCGACGGCCAAACAATGGGATTTGATTCGTCATTTGGAGAAAGAATTAAAAGAACTCGGACTGACAGACGTCGAGACGG
ATGAATACGGCTATCTATTCGCCACACTTCCAAGTAACGTCGATTATGACGTCCCGACAATCGGCTTCCTTGCCCACGTC
GATACGGCGACCGATTTCACAGGAACGAACGTCTCCCCACAACTCGTTGAGCATTATGAAGGTGGCGACATCGTCTTGAA
CGAGTCATTGAATGTCGTTTTATCACCAAAAGACTTCCCGGAACTGGACGGGTATGTCGGGCATACGCTCATGACAACGG
ACGGGACGACACTCCTCGGCGCGGACGATAAAGCGGGAATCGCCGAAATCGTCACAGCGATCGAATACTTATTGGCTCAC
CCCGAGATTCCACACGGGCCGGTCCGCATCGCCTTCACACCGGATGAAGAAATCGGACGTGGACCACATAAATTCGATGT
CGCCCGTTTTAACGCAGACTTCGCTTATACGATGGATGGCGGTCCACTCGGAGAGTTGCAGTATGAGAGCTTTAACGCGG
CCGGTGCGACCGTCACCTTCCACGGGACGAACGTGCACCCAGGTAGCGCCAAAAACAAGATGGTGAACTCGATGAAGCTC
GCGATGGCGTTCCAAAACCGTTTGCCTGCTGATGAGGCACCGGAACGGACGAGCAACTACGAAGGATTCTTCCACTTGAA
CGGCTTCAATGGGGACGTCGAGAAGACGACGCTTCAATACATCATCCGCGATCACGACAAACAGAAGTTCGAAGCGCGTA
AAGCGTTGATGGAGAAACTTGTCCTCGAATGGAAACAAAAATACGGGGAAGAACGGATTGAACTCCAGATGGAAGACCAG
TATTACAACATGGCTGAGAAAATCGAGCCGGTCAAACACATCGTGGATACCGTCGCTGATGTCATGCGTGGTCTTGGCAT
CGAACCGAAGATCGAGCCGATTCGTGGTGGGACAGACGGTTCGCAACTTTCTTACATGGGACTCCCGACGCCGAACATCT
TCACGGGTGGGGAGAACTATCACGGGAAGTTCGAATATGTCTCTGTGAATAACATGGAAAAAGCGACAACAGTCATCATC
GAAACACTTCGCACATTCGCGAAACGTGCACAAGCATAA

Upstream 100 bases:

>100_bases
TGAACAGGTCGCGTCCATCGATGGGCGCGACTTTTTTGTTTCGACTTCGATTGCGCGTGATAAACTAATGAAGATGAAAC
TTATTAGAGGAGGGCAATCC

Downstream 100 bases:

>100_bases
CACATGCGCCTCCAATCGTTCTCTTTTACACTAGAGACATTGGAGGCGATTTTTGATGAAACATACATTTAGCATGAACC
TCGGCTGGGAGGGCGGACGA

Product: peptidase T

Products: NA

Alternate protein names: Aminotripeptidase; Tripeptidase; Tripeptide aminopeptidase

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MQAKLIERLMTYAKIDTQSDFTSTTTPSTAKQWDLIRHLEKELKELGLTDVETDEYGYLFATLPSNVDYDVPTIGFLAHV
DTATDFTGTNVSPQLVEHYEGGDIVLNESLNVVLSPKDFPELDGYVGHTLMTTDGTTLLGADDKAGIAEIVTAIEYLLAH
PEIPHGPVRIAFTPDEEIGRGPHKFDVARFNADFAYTMDGGPLGELQYESFNAAGATVTFHGTNVHPGSAKNKMVNSMKL
AMAFQNRLPADEAPERTSNYEGFFHLNGFNGDVEKTTLQYIIRDHDKQKFEARKALMEKLVLEWKQKYGEERIELQMEDQ
YYNMAEKIEPVKHIVDTVADVMRGLGIEPKIEPIRGGTDGSQLSYMGLPTPNIFTGGENYHGKFEYVSVNNMEKATTVII
ETLRTFAKRAQA

Sequences:

>Translated_412_residues
MQAKLIERLMTYAKIDTQSDFTSTTTPSTAKQWDLIRHLEKELKELGLTDVETDEYGYLFATLPSNVDYDVPTIGFLAHV
DTATDFTGTNVSPQLVEHYEGGDIVLNESLNVVLSPKDFPELDGYVGHTLMTTDGTTLLGADDKAGIAEIVTAIEYLLAH
PEIPHGPVRIAFTPDEEIGRGPHKFDVARFNADFAYTMDGGPLGELQYESFNAAGATVTFHGTNVHPGSAKNKMVNSMKL
AMAFQNRLPADEAPERTSNYEGFFHLNGFNGDVEKTTLQYIIRDHDKQKFEARKALMEKLVLEWKQKYGEERIELQMEDQ
YYNMAEKIEPVKHIVDTVADVMRGLGIEPKIEPIRGGTDGSQLSYMGLPTPNIFTGGENYHGKFEYVSVNNMEKATTVII
ETLRTFAKRAQA
>Mature_412_residues
MQAKLIERLMTYAKIDTQSDFTSTTTPSTAKQWDLIRHLEKELKELGLTDVETDEYGYLFATLPSNVDYDVPTIGFLAHV
DTATDFTGTNVSPQLVEHYEGGDIVLNESLNVVLSPKDFPELDGYVGHTLMTTDGTTLLGADDKAGIAEIVTAIEYLLAH
PEIPHGPVRIAFTPDEEIGRGPHKFDVARFNADFAYTMDGGPLGELQYESFNAAGATVTFHGTNVHPGSAKNKMVNSMKL
AMAFQNRLPADEAPERTSNYEGFFHLNGFNGDVEKTTLQYIIRDHDKQKFEARKALMEKLVLEWKQKYGEERIELQMEDQ
YYNMAEKIEPVKHIVDTVADVMRGLGIEPKIEPIRGGTDGSQLSYMGLPTPNIFTGGENYHGKFEYVSVNNMEKATTVII
ETLRTFAKRAQA

Specific function: Cleaves the N-terminal amino acid of tripeptides

COG id: COG2195

COG function: function code E; Di- and tripeptidases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20B family

Homologues:

Organism=Escherichia coli, GI1787372, Length=411, Percent_Identity=52.5547445255474, Blast_Score=432, Evalue=1e-122,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PEPT_EXISA (C4L0I9)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002886297.1
- ProteinModelPortal:   C4L0I9
- GeneID:   7870026
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_1928
- OMA:   HMDTAPD
- GO:   GO:0005737
- GO:   GO:0006508
- HAMAP:   MF_00550
- InterPro:   IPR001261
- InterPro:   IPR002933
- InterPro:   IPR011650
- InterPro:   IPR010161
- PIRSF:   PIRSF037215
- TIGRFAMs:   TIGR01882

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.4.11.4

Molecular weight: Translated: 45991; Mature: 45991

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 81-81 ACT_SITE 176-176

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAKLIERLMTYAKIDTQSDFTSTTTPSTAKQWDLIRHLEKELKELGLTDVETDEYGYLF
CCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
ATLPSNVDYDVPTIGFLAHVDTATDFTGTNVSPQLVEHYEGGDIVLNESLNVVLSPKDFP
EECCCCCCCCCCCCHHEEEECCCCCCCCCCCCHHHHHHCCCCCEEEECCCEEEECCCCCC
ELDGYVGHTLMTTDGTTLLGADDKAGIAEIVTAIEYLLAHPEIPHGPVRIAFTPDEEIGR
CCCCCCCCEEEECCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHCC
GPHKFDVARFNADFAYTMDGGPLGELQYESFNAAGATVTFHGTNVHPGSAKNKMVNSMKL
CCCEEEEEEECCCEEEEECCCCCCCEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHH
AMAFQNRLPADEAPERTSNYEGFFHLNGFNGDVEKTTLQYIIRDHDKQKFEARKALMEKL
HHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VLEWKQKYGEERIELQMEDQYYNMAEKIEPVKHIVDTVADVMRGLGIEPKIEPIRGGTDG
HHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
SQLSYMGLPTPNIFTGGENYHGKFEYVSVNNMEKATTVIIETLRTFAKRAQA
CCEEEECCCCCCEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQAKLIERLMTYAKIDTQSDFTSTTTPSTAKQWDLIRHLEKELKELGLTDVETDEYGYLF
CCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
ATLPSNVDYDVPTIGFLAHVDTATDFTGTNVSPQLVEHYEGGDIVLNESLNVVLSPKDFP
EECCCCCCCCCCCCHHEEEECCCCCCCCCCCCHHHHHHCCCCCEEEECCCEEEECCCCCC
ELDGYVGHTLMTTDGTTLLGADDKAGIAEIVTAIEYLLAHPEIPHGPVRIAFTPDEEIGR
CCCCCCCCEEEECCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHCC
GPHKFDVARFNADFAYTMDGGPLGELQYESFNAAGATVTFHGTNVHPGSAKNKMVNSMKL
CCCEEEEEEECCCEEEEECCCCCCCEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHH
AMAFQNRLPADEAPERTSNYEGFFHLNGFNGDVEKTTLQYIIRDHDKQKFEARKALMEKL
HHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VLEWKQKYGEERIELQMEDQYYNMAEKIEPVKHIVDTVADVMRGLGIEPKIEPIRGGTDG
HHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
SQLSYMGLPTPNIFTGGENYHGKFEYVSVNNMEKATTVIIETLRTFAKRAQA
CCEEEECCCCCCEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA