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The map label for this gene is frwD [H]

Identifier: 229013119

GI number: 229013119

Start: 4201033

End: 4201377

Strand: Direct

Name: frwD [H]

Synonym: y3780

Alternate gene names: 229013119

Gene position: 4201033-4201377 (Clockwise)

Preceding gene: 22127650

Following gene: 22127654

Centisome position: 91.31

GC content: 44.64

Gene sequence:

>345_bases
ATGACAATTAAGCTGGTCGCGGTAACGGCCTGTGTCAGTGGCGTCGCACATACCTACATGGTCGCGGAGCGGTTAGACAA
ACTGTGCTTACAGCTTAAATGGCAACTAAAAGTTGAGACACAAGGTGCATTGGGTGTTGAGTACTCTCTCACTGAAGACG
ATATTTTGCAGGCAGATGCGGTGATATTGATAAACGACGTTGCGATTAAAGAGCAAGAGCGCTTTCAGAATTGCCGTTGT
GTTCAGGCCGATATACAAACTTTTTTATGTCATCCTGAGAAAATCCTCGCGGCAACCAAAAAAGTCATTTCTTCCCCCAA
AACCGTCAGCATTGTCATTAAATAA

Upstream 100 bases:

>100_bases
ACACCTAATCCCTGCGCTGCCAGCGATATCAACACCGTTGAGGCCGCAAAAAAATCAGGCCTGTTTGGAGACGAAACAGG
TGATCAAATCAGGAGGGGGC

Downstream 100 bases:

>100_bases
CATCTCTTTCCATCATATTACTGCGATATTGTTGGCGATATTCTGATGGTGTCAACTCAGTCTCTTTACGGAATAACCTA
CAAAAATAGTTGCTATCAAT

Product: putative fructose-like phosphotransferase EIIB subunit 3

Products: NA

Alternate protein names: PTS system fructose-like EIIB component 3 [H]

Number of amino acids: Translated: 114; Mature: 113

Protein sequence:

>114_residues
MTIKLVAVTACVSGVAHTYMVAERLDKLCLQLKWQLKVETQGALGVEYSLTEDDILQADAVILINDVAIKEQERFQNCRC
VQADIQTFLCHPEKILAATKKVISSPKTVSIVIK

Sequences:

>Translated_114_residues
MTIKLVAVTACVSGVAHTYMVAERLDKLCLQLKWQLKVETQGALGVEYSLTEDDILQADAVILINDVAIKEQERFQNCRC
VQADIQTFLCHPEKILAATKKVISSPKTVSIVIK
>Mature_113_residues
TIKLVAVTACVSGVAHTYMVAERLDKLCLQLKWQLKVETQGALGVEYSLTEDDILQADAVILINDVAIKEQERFQNCRCV
QADIQTFLCHPEKILAATKKVISSPKTVSIVIK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG1445

COG function: function code G; Phosphotransferase system fructose-specific component IIB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1790390, Length=110, Percent_Identity=52.7272727272727, Blast_Score=129, Evalue=3e-32,
Organism=Escherichia coli, GI1790387, Length=94, Percent_Identity=45.7446808510638, Blast_Score=91, Evalue=2e-20,
Organism=Escherichia coli, GI1786951, Length=107, Percent_Identity=38.3177570093458, Blast_Score=73, Evalue=4e-15,
Organism=Escherichia coli, GI1788730, Length=109, Percent_Identity=33.9449541284404, Blast_Score=68, Evalue=1e-13,
Organism=Escherichia coli, GI87082348, Length=73, Percent_Identity=43.8356164383562, Blast_Score=66, Evalue=6e-13,
Organism=Escherichia coli, GI1788492, Length=66, Percent_Identity=40.9090909090909, Blast_Score=62, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003353 [H]

Pfam domain/function: PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 12645; Mature: 12514

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS51099 PTS_EIIB_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
4.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKLVAVTACVSGVAHTYMVAERLDKLCLQLKWQLKVETQGALGVEYSLTEDDILQADA
CEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCEEEECCCCHHHCCCE
VILINDVAIKEQERFQNCRCVQADIQTFLCHPEKILAATKKVISSPKTVSIVIK
EEEEECHHHCHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEC
>Mature Secondary Structure 
TIKLVAVTACVSGVAHTYMVAERLDKLCLQLKWQLKVETQGALGVEYSLTEDDILQADA
EEEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCEEEECCCCHHHCCCE
VILINDVAIKEQERFQNCRCVQADIQTFLCHPEKILAATKKVISSPKTVSIVIK
EEEEECHHHCHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]