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The map label for this gene is frwB [H]

Identifier: 22127650

GI number: 22127650

Start: 4200620

End: 4200940

Strand: Direct

Name: frwB [H]

Synonym: y3778

Alternate gene names: 22127650

Gene position: 4200620-4200940 (Clockwise)

Preceding gene: 229013120

Following gene: 229013119

Centisome position: 91.3

GC content: 52.02

Gene sequence:

>321_bases
ATGACCAACATCATTGCTATCACCGCTTGCCCATCTGGCGTTGCCCACACTTACATGGCTGCCGAGGCGCTCGAACGGGC
GGCTAGAGCAAAAGGCTGGGAGTGCAAAGTCGAAACACAGGGGTCTATCGGGGTGGAAAATAAACTGACCGCGGAAGACG
TGGCTGCGGCGGATATGGTGATTCTGACCAAAGACATTGGCATCCTGTTTGAAGAGCGTTTTGCTGGCAAAGTCATCGTG
CGGATTGCTGTCAGCGACGCAGTAAAACGTGCTGATGCCATTATGGACAAGATTGCCGCCTATTTGGAAAAAAACACCTA
A

Upstream 100 bases:

>100_bases
TAGTCCCGATGAGCTTACTCAGGTAAGTGATTCGGGCGAGTGAGCGCAGCTAACACCCCTGTGGATTCAAGTACGAAGGG
GGGATTGGGAGGAAAACACC

Downstream 100 bases:

>100_bases
TCCCTGCGCTGCCAGCGATATCAACACCGTTGAGGCCGCAAAAAAATCAGGCCTGTTTGGAGACGAAACAGGTGATCAAA
TCAGGAGGGGGCATGACAAT

Product: putative PTS system fructose-like transporter subunit EIIB

Products: NA

Alternate protein names: PTS system fructose-like EIIB component 2 [H]

Number of amino acids: Translated: 106; Mature: 105

Protein sequence:

>106_residues
MTNIIAITACPSGVAHTYMAAEALERAARAKGWECKVETQGSIGVENKLTAEDVAAADMVILTKDIGILFEERFAGKVIV
RIAVSDAVKRADAIMDKIAAYLEKNT

Sequences:

>Translated_106_residues
MTNIIAITACPSGVAHTYMAAEALERAARAKGWECKVETQGSIGVENKLTAEDVAAADMVILTKDIGILFEERFAGKVIV
RIAVSDAVKRADAIMDKIAAYLEKNT
>Mature_105_residues
TNIIAITACPSGVAHTYMAAEALERAARAKGWECKVETQGSIGVENKLTAEDVAAADMVILTKDIGILFEERFAGKVIVR
IAVSDAVKRADAIMDKIAAYLEKNT

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG1445

COG function: function code G; Phosphotransferase system fructose-specific component IIB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1790387, Length=102, Percent_Identity=84.3137254901961, Blast_Score=177, Evalue=2e-46,
Organism=Escherichia coli, GI87082348, Length=97, Percent_Identity=54.639175257732, Blast_Score=101, Evalue=8e-24,
Organism=Escherichia coli, GI1788492, Length=94, Percent_Identity=50, Blast_Score=96, Evalue=6e-22,
Organism=Escherichia coli, GI1788730, Length=95, Percent_Identity=45.2631578947368, Blast_Score=89, Evalue=5e-20,
Organism=Escherichia coli, GI1790390, Length=98, Percent_Identity=39.7959183673469, Blast_Score=80, Evalue=2e-17,
Organism=Escherichia coli, GI1786951, Length=94, Percent_Identity=40.4255319148936, Blast_Score=80, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003353 [H]

Pfam domain/function: PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 11355; Mature: 11224

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS51099 PTS_EIIB_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNIIAITACPSGVAHTYMAAEALERAARAKGWECKVETQGSIGVENKLTAEDVAAADMV
CCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHEE
ILTKDIGILFEERFAGKVIVRIAVSDAVKRADAIMDKIAAYLEKNT
EEECCHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TNIIAITACPSGVAHTYMAAEALERAARAKGWECKVETQGSIGVENKLTAEDVAAADMV
CCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHEE
ILTKDIGILFEERFAGKVIVRIAVSDAVKRADAIMDKIAAYLEKNT
EEECCHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]