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The map label for this gene is lpdA [H]

Identifier: 227827159

GI number: 227827159

Start: 790591

End: 791814

Strand: Direct

Name: lpdA [H]

Synonym: M1425_0839

Alternate gene names: 227827159

Gene position: 790591-791814 (Clockwise)

Preceding gene: 227827157

Following gene: 227827160

Centisome position: 30.3

GC content: 36.36

Gene sequence:

>1224_bases
ATGAAAGTGGTAATAATTGGTGCAGGGCCAGCAGGAGTTTACTCAGCATTAACCTTATCCAAGCATGCAAAAGTCACACT
TATAGAAAAGGAAGAAAAACTGGGGGGTACGTGCGTACTTTACGGGTGTATACCAACAAAATCCATATTAAGCCAGCTTA
TCATATCTAGGCAAGCATCCAATATGAGTTTAAATACGTTTAGAGAGTATGCATTAGCTTCAATAAATACTATAAGTAAG
AGTTTAGAACAGTTACTAAATAGTCATGGGATAGAGGTTATTCACGCTAATGGTTTTCTAAGGTCGTCTATGGTTCATGC
ATCAGATACTTCACTTACGGCTGATAAGGTTTTAGTTTCAACCGGGACCAGAAGAGAAAGATTAGGAAAGGTAAAATTCA
CGGAAGATTTAGCCTATACTAATGAGGATTATAATAAGGTGGTTATAGTAGGAGGAGATGCAGGGGGAATAGAACTAGCT
TGGATGATGAAGAAACTGGGTAAGGAGGTACATCTTATAGACAAGAACGATTTACTTTTACAAAACATTGATAATACACT
ATCTGAGGTAGTTACAAATTTTCTAAGCCGTATCGGAATTAAATTATACTTAGGTAAAAAGGTCTCTAAAATTGATGAAA
CTTCTGTTACCCTTGAAGATAACCAAACGATCTCAGGCGATGCTGTATTTGTAACCTTCGGTCGTAAACCAAATATTGAA
GGATTTGAGGAAATCCCCCACGAGAAGTACATATACGTCGATGAATATTTGAGAACTCAAATTCCCAATATCTACGCAGC
AGGAGATATAATAGGTACGTTTACTGCACATGAAGCCATATATGGTGGTATTATAGCTGCGAAAAATATGTTAGGCGAAA
AAAGAGAGTTCCTCGTAGAAGGAATACCTAAGGTTATATACATTTACCCCCAAATAGCCTATGTAGGGACTACTATTGGT
AATTGTGTTACTTTCAACACATTAAATTTAACCAGAACAGTAGTAGAAAGAGAAGGTGAGGGATTCTTAAAGATATGCGA
AAGGGATAACAGAGTAATTGGGGGAGTAGCATTCATGCCTTATGCCGAAGACGTAATTTCTCTTATTTCAGTATTAATAC
GATATCAAATAAATTTAAAGGATACTATAGATCTTGTTATGCCTCATCCTTCTTATCTGGAAGCCATTACTGAGGCTTTA
AATATGCTGCAAAGTAAAGAGTAA

Upstream 100 bases:

>100_bases
ATGGTTCAACCATAATATTGAATGAACAAGTGAAAGTAGAGGAAGAACTGATCGAAAATATTGTATAGTATTTATATTCG
AGAAATTGAAGAATAATATT

Downstream 100 bases:

>100_bases
AGAACTGTTAATTGCTCAGTGAAGCTCATAGTGTTATTTTAACTTATTTATTTTTCTATGGTGAGTAGCATTTATTGGAA
AATCGTTGTATTCCATAATC

Product: FAD-dependent pyridine nucleotide-disulphide oxidoreductase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MKVVIIGAGPAGVYSALTLSKHAKVTLIEKEEKLGGTCVLYGCIPTKSILSQLIISRQASNMSLNTFREYALASINTISK
SLEQLLNSHGIEVIHANGFLRSSMVHASDTSLTADKVLVSTGTRRERLGKVKFTEDLAYTNEDYNKVVIVGGDAGGIELA
WMMKKLGKEVHLIDKNDLLLQNIDNTLSEVVTNFLSRIGIKLYLGKKVSKIDETSVTLEDNQTISGDAVFVTFGRKPNIE
GFEEIPHEKYIYVDEYLRTQIPNIYAAGDIIGTFTAHEAIYGGIIAAKNMLGEKREFLVEGIPKVIYIYPQIAYVGTTIG
NCVTFNTLNLTRTVVEREGEGFLKICERDNRVIGGVAFMPYAEDVISLISVLIRYQINLKDTIDLVMPHPSYLEAITEAL
NMLQSKE

Sequences:

>Translated_407_residues
MKVVIIGAGPAGVYSALTLSKHAKVTLIEKEEKLGGTCVLYGCIPTKSILSQLIISRQASNMSLNTFREYALASINTISK
SLEQLLNSHGIEVIHANGFLRSSMVHASDTSLTADKVLVSTGTRRERLGKVKFTEDLAYTNEDYNKVVIVGGDAGGIELA
WMMKKLGKEVHLIDKNDLLLQNIDNTLSEVVTNFLSRIGIKLYLGKKVSKIDETSVTLEDNQTISGDAVFVTFGRKPNIE
GFEEIPHEKYIYVDEYLRTQIPNIYAAGDIIGTFTAHEAIYGGIIAAKNMLGEKREFLVEGIPKVIYIYPQIAYVGTTIG
NCVTFNTLNLTRTVVEREGEGFLKICERDNRVIGGVAFMPYAEDVISLISVLIRYQINLKDTIDLVMPHPSYLEAITEAL
NMLQSKE
>Mature_407_residues
MKVVIIGAGPAGVYSALTLSKHAKVTLIEKEEKLGGTCVLYGCIPTKSILSQLIISRQASNMSLNTFREYALASINTISK
SLEQLLNSHGIEVIHANGFLRSSMVHASDTSLTADKVLVSTGTRRERLGKVKFTEDLAYTNEDYNKVVIVGGDAGGIELA
WMMKKLGKEVHLIDKNDLLLQNIDNTLSEVVTNFLSRIGIKLYLGKKVSKIDETSVTLEDNQTISGDAVFVTFGRKPNIE
GFEEIPHEKYIYVDEYLRTQIPNIYAAGDIIGTFTAHEAIYGGIIAAKNMLGEKREFLVEGIPKVIYIYPQIAYVGTTIG
NCVTFNTLNLTRTVVEREGEGFLKICERDNRVIGGVAFMPYAEDVISLISVLIRYQINLKDTIDLVMPHPSYLEAITEAL
NMLQSKE

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=459, Percent_Identity=24.8366013071895, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI50301238, Length=435, Percent_Identity=23.2183908045977, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI1786307, Length=455, Percent_Identity=23.7362637362637, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI87082354, Length=354, Percent_Identity=25.7062146892655, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1789915, Length=334, Percent_Identity=29.940119760479, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87081717, Length=448, Percent_Identity=23.2142857142857, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI32565766, Length=456, Percent_Identity=24.3421052631579, Blast_Score=132, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6321091, Length=466, Percent_Identity=24.2489270386266, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6325166, Length=362, Percent_Identity=24.585635359116, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6325240, Length=472, Percent_Identity=23.3050847457627, Blast_Score=83, Evalue=8e-17,
Organism=Drosophila melanogaster, GI21358499, Length=460, Percent_Identity=26.0869565217391, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI17737741, Length=489, Percent_Identity=21.4723926380368, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR002218
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999 [H]

Pfam domain/function: PF01134 GIDA; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 44995; Mature: 44995

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVIIGAGPAGVYSALTLSKHAKVTLIEKEEKLGGTCVLYGCIPTKSILSQLIISRQAS
CEEEEEECCCHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHCCC
NMSLNTFREYALASINTISKSLEQLLNSHGIEVIHANGFLRSSMVHASDTSLTADKVLVS
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHCEEECCCCCCCHHHEEEC
TGTRRERLGKVKFTEDLAYTNEDYNKVVIVGGDAGGIELAWMMKKLGKEVHLIDKNDLLL
CCCCHHHCCCCEEEHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHCCEEEEECCCCHHH
QNIDNTLSEVVTNFLSRIGIKLYLGKKVSKIDETSVTLEDNQTISGDAVFVTFGRKPNIE
HHHHHHHHHHHHHHHHHCCEEEEECCHHHCCCCCEEEEECCCEECCCEEEEEECCCCCCC
GFEEIPHEKYIYVDEYLRTQIPNIYAAGDIIGTFTAHEAIYGGIIAAKNMLGEKREFLVE
CHHHCCCCCEEEHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIPKVIYIYPQIAYVGTTIGNCVTFNTLNLTRTVVEREGEGFLKICERDNRVIGGVAFMP
CCCEEEEEEECHHHHHCCCCCEEEEEECHHHHHHHHCCCCCCEEHCCCCCEEEEEEEECC
YAEDVISLISVLIRYQINLKDTIDLVMPHPSYLEAITEALNMLQSKE
CHHHHHHHHHHHHHEECCCCCCEEEEECCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVVIIGAGPAGVYSALTLSKHAKVTLIEKEEKLGGTCVLYGCIPTKSILSQLIISRQAS
CEEEEEECCCHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHCCC
NMSLNTFREYALASINTISKSLEQLLNSHGIEVIHANGFLRSSMVHASDTSLTADKVLVS
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHCEEECCCCCCCHHHEEEC
TGTRRERLGKVKFTEDLAYTNEDYNKVVIVGGDAGGIELAWMMKKLGKEVHLIDKNDLLL
CCCCHHHCCCCEEEHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHCCEEEEECCCCHHH
QNIDNTLSEVVTNFLSRIGIKLYLGKKVSKIDETSVTLEDNQTISGDAVFVTFGRKPNIE
HHHHHHHHHHHHHHHHHCCEEEEECCHHHCCCCCEEEEECCCEECCCEEEEEECCCCCCC
GFEEIPHEKYIYVDEYLRTQIPNIYAAGDIIGTFTAHEAIYGGIIAAKNMLGEKREFLVE
CHHHCCCCCEEEHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIPKVIYIYPQIAYVGTTIGNCVTFNTLNLTRTVVEREGEGFLKICERDNRVIGGVAFMP
CCCEEEEEEECHHHHHCCCCCEEEEEECHHHHHHHHCCCCCCEEHCCCCCEEEEEEEECC
YAEDVISLISVLIRYQINLKDTIDLVMPHPSYLEAITEALNMLQSKE
CHHHHHHHHHHHHHEECCCCCCEEEEECCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]