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The map label for this gene is rffG [C]

Identifier: 227826986

GI number: 227826986

Start: 588901

End: 589824

Strand: Direct

Name: rffG [C]

Synonym: M1425_0643

Alternate gene names: 227826986

Gene position: 588901-589824 (Clockwise)

Preceding gene: 227826985

Following gene: 227826987

Centisome position: 22.57

GC content: 33.23

Gene sequence:

>924_bases
ATGAAGTTTCTTATAAGTGGCGGTGCTGGGTTTCTAGGCTCTCATCTCATAGAAAACTTGGCTAATGAACATGAAATAAC
AATAGTAGATGATTTATCAACTACAAAATATATACAACTACCAAAAAATGTGAAATTGATAAAAGACAAAATAGAGAATT
TTAAAACAAATGAAAAATTTGACTACATCCTACATTTAGCGGCAAGGCCATCACCAGAAGATTACATGAACAATCCAATA
GAAACATTGTTATCAAACTCCATAGGGACATGGAATGCATTAGAAATAGCTAGAAAGAGTGATGCAATATTTATGTATAC
TTCTTCCTCAGAAATTTACGGCAATGCAGAAGTATTACCAATACCAGAAGAATATTGGGGAAAAGTAAACCCTATTGGAG
TTAGAAGTTGTTATGATGAAGGAAAAAGATTCTCGGAAGCATTAACAATGGCATATTATAGGGAATATGGATTAGACGTT
AGAATACAGAGACCATTTAACGTTTATGGGCCTAGATTGAGAGAAGATGGAAACTATGGAAGAGTAATATCACGTTTTAT
ATATCAAGCATTAAGAGGAGAAGACATTACAGTCTTTGGAGATGGTAAACAAACTAGGGCATTCTTATACGTTACAGATT
GGGTTGAAGCTACGAAAAAGCTTTTATTTAGCAAGGGAATAAAGGGCATAGTGTTAAATATTGGCTCAGATAAAGAGGTT
AAGATAATAGAGTTAGCTAGAATGATAATAAATCTAACTAATAGTAAATCTAATATAAAATTCCTTCCACCAAGACCAGA
TGATCCTTCAAGAAGGGCTGCAGATATAACAAAAGCTAAAAAGTTGCTAAATTGGGAGCCTAAATTATCCTTAGAAGAAG
GGTTAAGGAAAACAATAGATTGGTTTAGGGGTGTAATTGAATGA

Upstream 100 bases:

>100_bases
AAAAGTATTATCTATCAAAATTTTTAGCTATATGGAAATTTTTATTATATCTAAATAATTCAAAGCAAAGTTTATCTTAT
ACTTAACATATGTATATTTA

Downstream 100 bases:

>100_bases
TAATAGGTATAGTAGGCTTAGGCTATGTAGGATTAGTAACTGGTGCAGTTTTAGCTGATCAAGGACATTACGTAATAGGT
GTCGATATAGACGAGAAAAA

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: Galactowaldenase; UDP-galactose 4-epimerase [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MKFLISGGAGFLGSHLIENLANEHEITIVDDLSTTKYIQLPKNVKLIKDKIENFKTNEKFDYILHLAARPSPEDYMNNPI
ETLLSNSIGTWNALEIARKSDAIFMYTSSSEIYGNAEVLPIPEEYWGKVNPIGVRSCYDEGKRFSEALTMAYYREYGLDV
RIQRPFNVYGPRLREDGNYGRVISRFIYQALRGEDITVFGDGKQTRAFLYVTDWVEATKKLLFSKGIKGIVLNIGSDKEV
KIIELARMIINLTNSKSNIKFLPPRPDDPSRRAADITKAKKLLNWEPKLSLEEGLRKTIDWFRGVIE

Sequences:

>Translated_307_residues
MKFLISGGAGFLGSHLIENLANEHEITIVDDLSTTKYIQLPKNVKLIKDKIENFKTNEKFDYILHLAARPSPEDYMNNPI
ETLLSNSIGTWNALEIARKSDAIFMYTSSSEIYGNAEVLPIPEEYWGKVNPIGVRSCYDEGKRFSEALTMAYYREYGLDV
RIQRPFNVYGPRLREDGNYGRVISRFIYQALRGEDITVFGDGKQTRAFLYVTDWVEATKKLLFSKGIKGIVLNIGSDKEV
KIIELARMIINLTNSKSNIKFLPPRPDDPSRRAADITKAKKLLNWEPKLSLEEGLRKTIDWFRGVIE
>Mature_307_residues
MKFLISGGAGFLGSHLIENLANEHEITIVDDLSTTKYIQLPKNVKLIKDKIENFKTNEKFDYILHLAARPSPEDYMNNPI
ETLLSNSIGTWNALEIARKSDAIFMYTSSSEIYGNAEVLPIPEEYWGKVNPIGVRSCYDEGKRFSEALTMAYYREYGLDV
RIQRPFNVYGPRLREDGNYGRVISRFIYQALRGEDITVFGDGKQTRAFLYVTDWVEATKKLLFSKGIKGIVLNIGSDKEV
KIIELARMIINLTNSKSNIKFLPPRPDDPSRRAADITKAKKLLNWEPKLSLEEGLRKTIDWFRGVIE

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=313, Percent_Identity=46.6453674121406, Blast_Score=271, Evalue=6e-73,
Organism=Homo sapiens, GI7657641, Length=328, Percent_Identity=30.4878048780488, Blast_Score=134, Evalue=9e-32,
Organism=Escherichia coli, GI48994969, Length=337, Percent_Identity=29.3768545994065, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI1788353, Length=342, Percent_Identity=26.3157894736842, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI1788589, Length=336, Percent_Identity=25.5952380952381, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788365, Length=326, Percent_Identity=26.3803680981595, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1786974, Length=335, Percent_Identity=24.7761194029851, Blast_Score=85, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17539532, Length=314, Percent_Identity=42.0382165605096, Blast_Score=242, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI17568069, Length=327, Percent_Identity=28.4403669724771, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI115532424, Length=331, Percent_Identity=29.3051359516616, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI71982035, Length=340, Percent_Identity=22.6470588235294, Blast_Score=71, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=338, Percent_Identity=23.9644970414201, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21356223, Length=308, Percent_Identity=46.4285714285714, Blast_Score=264, Evalue=6e-71,
Organism=Drosophila melanogaster, GI19923002, Length=338, Percent_Identity=23.3727810650888, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19922778, Length=318, Percent_Identity=22.9559748427673, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.2 [H]

Molecular weight: Translated: 35113; Mature: 35113

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFLISGGAGFLGSHLIENLANEHEITIVDDLSTTKYIQLPKNVKLIKDKIENFKTNEKF
CEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEECCCCHHHHHHHHHCCCCCCCE
DYILHLAARPSPEDYMNNPIETLLSNSIGTWNALEIARKSDAIFMYTSSSEIYGNAEVLP
EEEEEEECCCCHHHHHCCHHHHHHCCCCCCCHHHHHHCCCCEEEEEECCCCEECCCEEEE
IPEEYWGKVNPIGVRSCYDEGKRFSEALTMAYYREYGLDVRIQRPFNVYGPRLREDGNYG
CCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCHH
RVISRFIYQALRGEDITVFGDGKQTRAFLYVTDWVEATKKLLFSKGIKGIVLNIGSDKEV
HHHHHHHHHHHCCCCEEEEECCCCEEEEEEEEHHHHHHHHHHHHCCCCEEEEECCCCCCC
KIIELARMIINLTNSKSNIKFLPPRPDDPSRRAADITKAKKLLNWEPKLSLEEGLRKTID
HHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
WFRGVIE
HHHHHCC
>Mature Secondary Structure
MKFLISGGAGFLGSHLIENLANEHEITIVDDLSTTKYIQLPKNVKLIKDKIENFKTNEKF
CEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEECCCCHHHHHHHHHCCCCCCCE
DYILHLAARPSPEDYMNNPIETLLSNSIGTWNALEIARKSDAIFMYTSSSEIYGNAEVLP
EEEEEEECCCCHHHHHCCHHHHHHCCCCCCCHHHHHHCCCCEEEEEECCCCEECCCEEEE
IPEEYWGKVNPIGVRSCYDEGKRFSEALTMAYYREYGLDVRIQRPFNVYGPRLREDGNYG
CCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCHH
RVISRFIYQALRGEDITVFGDGKQTRAFLYVTDWVEATKKLLFSKGIKGIVLNIGSDKEV
HHHHHHHHHHHCCCCEEEEECCCCEEEEEEEEHHHHHHHHHHHHCCCCEEEEECCCCCCC
KIIELARMIINLTNSKSNIKFLPPRPDDPSRRAADITKAKKLLNWEPKLSLEEGLRKTID
HHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
WFRGVIE
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]