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The map label for this gene is yheN [H]

Identifier: 227815203

GI number: 227815203

Start: 2376792

End: 2377613

Strand: Reverse

Name: yheN [H]

Synonym: BAMEG_2614

Alternate gene names: 227815203

Gene position: 2377613-2376792 (Counterclockwise)

Preceding gene: 227815204

Following gene: 227815202

Centisome position: 45.46

GC content: 35.89

Gene sequence:

>822_bases
ATGGAAAAAGCTTTTAAAATTAAACGAGTAGTATTCGTTTTAATAGCGATTGCAGCAGTAGCAATTGGGTATTTCATGTT
TCAATCCATTACTTCACCAGCAAAGGCTGTTGCAAAACAAGAAAATGTAGTACAACTCGCAAGTGAACAACCAAAAGTAG
AAATGAATAAAACGGCACCAAGTCGTTTTAATGGCAAGGAAAGGAAAGTTGCTTATCTTACATTTGATGATGGCCCAGGG
AAATATACGGCTGAATTATTAAATACGTTAAAACAACATGATGCGAAAGCGACATTCTTTTTAATTGGAGCGAATGTAAA
AGAGTTTCCAGATTTAGTGAAACGAGAAAATGCAGAAGGTCATTATGTAGGAATGCATAGTATGACGCATAATTTTGCAA
AGTTATATAAGAATGGCGAGTATGTAAATGAGATGAAAGAGGATCAAAGCTTAATCGCCAATATTATTGGGAAATCACCT
AAATTAACACGTCCACCATACGGTTCGATGCCAGGATTAAATGAAGGTCTTCGAAATAAAGTGGTAGAAGGCGGCTTTAA
AGTGTGGGATTGGACAATTGATTCATTAGACTGGAAATATAACAAAATGCAAGTTGATGCAGCTGCAGCACAAATTGCTC
AAAATGTATTAACAAATGCAACAAAACCACAAGAAGTCATTTTAATGCATGACATTCATCCGCAATCAGTAGCTGCTGTG
CCAGCAATTTTAAAAGGGTTAAAAGAAAAGGGTTATGAGTTTGAAGCTTATCATGAAGAAAGTCACTTCCCAGTGAATTT
CTGGCATGATAACCGTATGTAA

Upstream 100 bases:

>100_bases
TACATATAAAGTCTGTTTATATAGTGTAATAAAATAAATAGATGTATAATTATATTGGGTGATTTATATATAAGATTAAT
AGGGAGAGGAATTCGATAGA

Downstream 100 bases:

>100_bases
CGGAGGAATGAACGTTGAAGTATGGAAAAGTAGCAGTAGTTGGAGCTCTATCAGTAGGACTATTAACAGGTTGCTTCGGT
GAAAAACCAGAAGAAAACCT

Product: putative polysaccharide deacetylase

Products: chitosan; acetate

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MEKAFKIKRVVFVLIAIAAVAIGYFMFQSITSPAKAVAKQENVVQLASEQPKVEMNKTAPSRFNGKERKVAYLTFDDGPG
KYTAELLNTLKQHDAKATFFLIGANVKEFPDLVKRENAEGHYVGMHSMTHNFAKLYKNGEYVNEMKEDQSLIANIIGKSP
KLTRPPYGSMPGLNEGLRNKVVEGGFKVWDWTIDSLDWKYNKMQVDAAAAQIAQNVLTNATKPQEVILMHDIHPQSVAAV
PAILKGLKEKGYEFEAYHEESHFPVNFWHDNRM

Sequences:

>Translated_273_residues
MEKAFKIKRVVFVLIAIAAVAIGYFMFQSITSPAKAVAKQENVVQLASEQPKVEMNKTAPSRFNGKERKVAYLTFDDGPG
KYTAELLNTLKQHDAKATFFLIGANVKEFPDLVKRENAEGHYVGMHSMTHNFAKLYKNGEYVNEMKEDQSLIANIIGKSP
KLTRPPYGSMPGLNEGLRNKVVEGGFKVWDWTIDSLDWKYNKMQVDAAAAQIAQNVLTNATKPQEVILMHDIHPQSVAAV
PAILKGLKEKGYEFEAYHEESHFPVNFWHDNRM
>Mature_273_residues
MEKAFKIKRVVFVLIAIAAVAIGYFMFQSITSPAKAVAKQENVVQLASEQPKVEMNKTAPSRFNGKERKVAYLTFDDGPG
KYTAELLNTLKQHDAKATFFLIGANVKEFPDLVKRENAEGHYVGMHSMTHNFAKLYKNGEYVNEMKEDQSLIANIIGKSP
KLTRPPYGSMPGLNEGLRNKVVEGGFKVWDWTIDSLDWKYNKMQVDAAAAQIAQNVLTNATKPQEVILMHDIHPQSVAAV
PAILKGLKEKGYEFEAYHEESHFPVNFWHDNRM

Specific function: Unknown

COG id: COG0726

COG function: function code G; Predicted xylanase/chitin deacetylase

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide deacetylase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6323338, Length=159, Percent_Identity=33.9622641509434, Blast_Score=83, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6323339, Length=158, Percent_Identity=30.379746835443, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011330
- InterPro:   IPR002509 [H]

Pfam domain/function: PF01522 Polysacc_deac_1 [H]

EC number: 3.5.1.41

Molecular weight: Translated: 30791; Mature: 30791

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKAFKIKRVVFVLIAIAAVAIGYFMFQSITSPAKAVAKQENVVQLASEQPKVEMNKTAP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEECCCCC
SRFNGKERKVAYLTFDDGPGKYTAELLNTLKQHDAKATFFLIGANVKEFPDLVKRENAEG
CCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHCCCCC
HYVGMHSMTHNFAKLYKNGEYVNEMKEDQSLIANIIGKSPKLTRPPYGSMPGLNEGLRNK
CEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
VVEGGFKVWDWTIDSLDWKYNKMQVDAAAAQIAQNVLTNATKPQEVILMHDIHPQSVAAV
HHHCCCEEEEEEECCCCCCCHHEEHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHH
PAILKGLKEKGYEFEAYHEESHFPVNFWHDNRM
HHHHHHHHHCCCEEEEEECCCCCCEEECCCCCC
>Mature Secondary Structure
MEKAFKIKRVVFVLIAIAAVAIGYFMFQSITSPAKAVAKQENVVQLASEQPKVEMNKTAP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEECCCCC
SRFNGKERKVAYLTFDDGPGKYTAELLNTLKQHDAKATFFLIGANVKEFPDLVKRENAEG
CCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHCCCCC
HYVGMHSMTHNFAKLYKNGEYVNEMKEDQSLIANIIGKSPKLTRPPYGSMPGLNEGLRNK
CEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
VVEGGFKVWDWTIDSLDWKYNKMQVDAAAAQIAQNVLTNATKPQEVILMHDIHPQSVAAV
HHHCCCEEEEEEECCCCCCCHHEEHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHH
PAILKGLKEKGYEFEAYHEESHFPVNFWHDNRM
HHHHHHHHHCCCEEEEEECCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: chitin; H2O

Specific reaction: chitin + H2O = chitosan + acetate

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]