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The map label for this gene is yfjD [H]

Identifier: 227080741

GI number: 227080741

Start: 549742

End: 551022

Strand: Reverse

Name: yfjD [H]

Synonym: VCM66_0516

Alternate gene names: 227080741

Gene position: 551022-549742 (Counterclockwise)

Preceding gene: 227080742

Following gene: 227080738

Centisome position: 19.05

GC content: 46.6

Gene sequence:

>1281_bases
TTGGACGACATATCAACGGGTATCTTATTTGCGCTACTCGCGTGTCTCATCATTATTTCCGCCTATTTCTCCGGTTCTGA
AACCGGCATGATGGCTCTCAACCGTTACCGTTTAAAGCATTTGGCCAACAGCGGCCATAAAGGTGCTAAACGTGTTGAAA
AGCTACTGGATCGTCCAGATCGTTTGATTGGCTTAATCCTGATTGGTAACAACCTCGTCAATATCCTCGCCTCTGCGATT
GCCACCATTATCGGTATGCGCCTGTATGGCGATTTAGGTGTCGCAATTGCCACGGGGGCCTTAACTTTAGTGATTCTGGT
GTTTGCCGAAGTCACGCCTAAAACTCTAGCCGCGCTTTACCCAGAGCGAGTCTCTTACGCCAGCAGCGTGTTACTGACGA
TTTTGATGAAGCTGCTTTCACCTTTGGTGCTGTTCGTTAACTTGATGACCAATGGCTTGATCCGCATTCTCGGCATCTCA
CCGAAACATGGCAAAGGCGATCATTTAAGCTCAGAAGAGTTACGTACTGTGGTTAATGAAGCGGGAGGACTCATCCCTCG
CCGCCACCAAGATATGCTGCTGTCGATCCTCGATTTAGAACACGTCACCGTCAACGACATCATGATCCCGCGTAATGAGA
TAACGGGGATCAATATCAATGATGATTGGAAGTCGATTGTGCGTCAGTTAGTCCATTCACCACATGGGCGAGTCGTACTG
TACCGTGACAAGATTGACGAAGTGGTCGGGATTTTGCGTTTACGTGAAGCCTCACGCTTTATGCTTGAGAAGAACGACTT
TAACAAAGAGATGCTGCTGCGCGCTGCGGATGAGATTTACTTTATCCCTGAAGGTACACCGCTTAACGTTCAGCTGCTCA
AATTCCAACGTAATAAAGAACGCATTGGTTTAATCGTGGATGAATACGGTGAAATCATTGGTTTAACCACGCTCGAAGAT
ATTTTGGAAGAGATTGTCGGTGAATTTACCACATCCATGTCTCCAAGCTTGGCCGATGAAATTACTCCACAAGGTGATGG
CAGTTTCTTGATTGAAGGCAGCGCCAATATTCGTGACATTAACAAGAGCCTGAAATGGAAGCTTCCTACCGATGGACCAC
GAACCTTAAACGGTTTGATTTTGGAGCACCTTGAAGAGATCCCAGCGAGCCATCTGAGTGTGAAAGTGGCACAGCACAAA
ATGGAAATCCTCGAGCTGGAAGAGAATCGGATTAAGTTGGTCAAAGTTTACCCAAGAAAAACCAAAGTCAGCTTGGTTTA
A

Upstream 100 bases:

>100_bases
GAAATCATTCTGCGCTGAACAAGATTCATCGAGCTGTCATTTGACACTCGCAATACATTCAGCCTTTAATTAGAACTCTG
TCATAAGGAAAATTCGCGTT

Downstream 100 bases:

>100_bases
GTTAAGCACTCAAACAAAAGCCCGAACTCAACTTCGGGCTTTTGTATTTTTAACGTTTACAGAACCGACTTAGTGAACCT
TCAGCTCATTGAGCATAGAT

Product: putative hemolysin

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MDDISTGILFALLACLIIISAYFSGSETGMMALNRYRLKHLANSGHKGAKRVEKLLDRPDRLIGLILIGNNLVNILASAI
ATIIGMRLYGDLGVAIATGALTLVILVFAEVTPKTLAALYPERVSYASSVLLTILMKLLSPLVLFVNLMTNGLIRILGIS
PKHGKGDHLSSEELRTVVNEAGGLIPRRHQDMLLSILDLEHVTVNDIMIPRNEITGININDDWKSIVRQLVHSPHGRVVL
YRDKIDEVVGILRLREASRFMLEKNDFNKEMLLRAADEIYFIPEGTPLNVQLLKFQRNKERIGLIVDEYGEIIGLTTLED
ILEEIVGEFTTSMSPSLADEITPQGDGSFLIEGSANIRDINKSLKWKLPTDGPRTLNGLILEHLEEIPASHLSVKVAQHK
MEILELEENRIKLVKVYPRKTKVSLV

Sequences:

>Translated_426_residues
MDDISTGILFALLACLIIISAYFSGSETGMMALNRYRLKHLANSGHKGAKRVEKLLDRPDRLIGLILIGNNLVNILASAI
ATIIGMRLYGDLGVAIATGALTLVILVFAEVTPKTLAALYPERVSYASSVLLTILMKLLSPLVLFVNLMTNGLIRILGIS
PKHGKGDHLSSEELRTVVNEAGGLIPRRHQDMLLSILDLEHVTVNDIMIPRNEITGININDDWKSIVRQLVHSPHGRVVL
YRDKIDEVVGILRLREASRFMLEKNDFNKEMLLRAADEIYFIPEGTPLNVQLLKFQRNKERIGLIVDEYGEIIGLTTLED
ILEEIVGEFTTSMSPSLADEITPQGDGSFLIEGSANIRDINKSLKWKLPTDGPRTLNGLILEHLEEIPASHLSVKVAQHK
MEILELEENRIKLVKVYPRKTKVSLV
>Mature_426_residues
MDDISTGILFALLACLIIISAYFSGSETGMMALNRYRLKHLANSGHKGAKRVEKLLDRPDRLIGLILIGNNLVNILASAI
ATIIGMRLYGDLGVAIATGALTLVILVFAEVTPKTLAALYPERVSYASSVLLTILMKLLSPLVLFVNLMTNGLIRILGIS
PKHGKGDHLSSEELRTVVNEAGGLIPRRHQDMLLSILDLEHVTVNDIMIPRNEITGININDDWKSIVRQLVHSPHGRVVL
YRDKIDEVVGILRLREASRFMLEKNDFNKEMLLRAADEIYFIPEGTPLNVQLLKFQRNKERIGLIVDEYGEIIGLTTLED
ILEEIVGEFTTSMSPSLADEITPQGDGSFLIEGSANIRDINKSLKWKLPTDGPRTLNGLILEHLEEIPASHLSVKVAQHK
MEILELEENRIKLVKVYPRKTKVSLV

Specific function: Unknown

COG id: COG4536

COG function: function code P; Putative Mg2+ and Co2+ transporter CorB

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 CBS domains [H]

Homologues:

Organism=Homo sapiens, GI310128564, Length=383, Percent_Identity=24.2819843342037, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI94681046, Length=335, Percent_Identity=24.7761194029851, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI40068053, Length=338, Percent_Identity=24.8520710059172, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI40068055, Length=338, Percent_Identity=24.8520710059172, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI145693175, Length=400, Percent_Identity=64.75, Blast_Score=548, Evalue=1e-157,
Organism=Escherichia coli, GI1790664, Length=418, Percent_Identity=26.0765550239234, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1786879, Length=254, Percent_Identity=24.8031496062992, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI87082033, Length=236, Percent_Identity=27.1186440677966, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788119, Length=237, Percent_Identity=27.4261603375527, Blast_Score=75, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016169
- InterPro:   IPR000644
- InterPro:   IPR002550
- InterPro:   IPR005170 [H]

Pfam domain/function: PF00571 CBS; PF03471 CorC_HlyC; PF01595 DUF21 [H]

EC number: NA

Molecular weight: Translated: 47408; Mature: 47408

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDISTGILFALLACLIIISAYFSGSETGMMALNRYRLKHLANSGHKGAKRVEKLLDRPD
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
RLIGLILIGNNLVNILASAIATIIGMRLYGDLGVAIATGALTLVILVFAEVTPKTLAALY
CEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
PERVSYASSVLLTILMKLLSPLVLFVNLMTNGLIRILGISPKHGKGDHLSSEELRTVVNE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHH
AGGLIPRRHQDMLLSILDLEHVTVNDIMIPRNEITGININDDWKSIVRQLVHSPHGRVVL
HCCCCCCHHHHHHHHHHHHCCEEECCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEE
YRDKIDEVVGILRLREASRFMLEKNDFNKEMLLRAADEIYFIPEGTPLNVQLLKFQRNKE
EHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEECCCCCCCEEEEEEECCCCC
RIGLIVDEYGEIIGLTTLEDILEEIVGEFTTSMSPSLADEITPQGDGSFLIEGSANIRDI
CCCEEHEHHCCEEEHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCEEEECCCCHHHH
NKSLKWKLPTDGPRTLNGLILEHLEEIPASHLSVKVAQHKMEILELEENRIKLVKVYPRK
CCCCEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
TKVSLV
CEEECC
>Mature Secondary Structure
MDDISTGILFALLACLIIISAYFSGSETGMMALNRYRLKHLANSGHKGAKRVEKLLDRPD
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
RLIGLILIGNNLVNILASAIATIIGMRLYGDLGVAIATGALTLVILVFAEVTPKTLAALY
CEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
PERVSYASSVLLTILMKLLSPLVLFVNLMTNGLIRILGISPKHGKGDHLSSEELRTVVNE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHH
AGGLIPRRHQDMLLSILDLEHVTVNDIMIPRNEITGININDDWKSIVRQLVHSPHGRVVL
HCCCCCCHHHHHHHHHHHHCCEEECCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEE
YRDKIDEVVGILRLREASRFMLEKNDFNKEMLLRAADEIYFIPEGTPLNVQLLKFQRNKE
EHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEECCCCCCCEEEEEEECCCCC
RIGLIVDEYGEIIGLTTLEDILEEIVGEFTTSMSPSLADEITPQGDGSFLIEGSANIRDI
CCCEEHEHHCCEEEHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCEEEECCCCHHHH
NKSLKWKLPTDGPRTLNGLILEHLEEIPASHLSVKVAQHKMEILELEENRIKLVKVYPRK
CCCCEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
TKVSLV
CEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3045760 [H]