Click here to switch to the map view.

The map label for this gene is cadB [H]

Identifier: 227080494

GI number: 227080494

Start: 270379

End: 271716

Strand: Direct

Name: cadB [H]

Synonym: VCM66_0265

Alternate gene names: 227080494

Gene position: 270379-271716 (Clockwise)

Preceding gene: 227080493

Following gene: 227080495

Centisome position: 9.35

GC content: 51.2

Gene sequence:

>1338_bases
ATGTCATCAAATGCAAAAAAAATCGGCCTGATCGCCTGTACGGGTGTGGTGGCTGGCAACATGATGGGCAGTGGTATTGC
GCTGCTTCCTTCTACGCTTGCGAGCGTAGGTTCAATTTCCATTTATAGCTGGCTGATCTGCATTGTTGGCGCACTCAGTT
TGGCGTTTGTGTTTGCTCGTTTGGCAACCAAAAACCCGCAAGAGGGTGGCCCGATTGCTTATGCAGGGGAAGTGTCACCG
GTGTTTGGTTTCCAAACGGGCGTGCTTTACTACCACGCTAACTGGATTGGTAATTTGGCGATTGCGATTACCGGTGTTTC
TTACCTTTCGGTGTTCTTCCCGGTGCTGAATAACCCGATCCCTGCTGGTTTAGCGACCATTGCTTCGGTTTGGCTATTTA
CCTTAGTCAACTTGCTTGGTGGTAGCTGGGTAAGCCGTTTATGTACCATTGGTTTGGTGCTGATTTTGATCCCTGTAGTC
GGTACGGCTCTGTTTGGTTGGACGCACTTTGACAGCGCGCTCTACAGCCAAAACTGGAACGTGTCTGCCGGTAGCGATGG
CCATGCGGTGATCACTGCAGTGCTGATCTGTCTGTGGTCTTTCGTGGGTGTTGAATCGGCAGCGGTTTCTTCCGGTATGG
TCGAAAACCCAAAACGCACGGTGCCACTGGCAACCATGTTAGGTACTGGTCTTGCGGGCCTTATCTATGTGCTATCAACT
CAAATGATCAGCGGTATGTTCCCTGCCTCTGAAGTGGCGGCATCGGGTGCACCTTTTGCACTGGCGACCACAGCACTGTT
TGGTAGCTGGACTGCGCCATTCGTGTCTGCCTTTACTGCACTAGCGTGTTTCACGTCACTTGGCTCTTGGATGATGCTGG
TCGGCGAAGCGGGCAAACGTGCCGCTAACGATGGCAACTTCCCGAAAGTATTCGGTGAAACCGATCGCAACGGTGTACCG
AAAAAAGGTCTGCTGATTGCTTCCAGCATGATGACATTGCTGATGCTGGTGCTGATGTTCTTCAGCTCAGAAACTGCGCA
CGCTTCCGATCTGTTTAACCAACTGACTACCGATGCGGTTCTGCTGACCATGCTGCCTTATTTCTACTCCAGCATTAACC
TGATCCGCTTCGAAGGCATGACCACTCGCAATACGTTTGTGATGCTGTTCTCCGGTATTGCCAGCCTGTTCTGTATGGTG
GCGCTGGCTGGTGCAGAGGGTTCAACCCTGACTGCAACCTTCATCATGTCGCTGATTATTTTGATGTTCTACAGCAAAAA
AGCGGGTCTGGATAAATACCTCGAGACTCATCCACAACAAGCTTCGGCTCAGTTCTGA

Upstream 100 bases:

>100_bases
CGAAATTTAGCGAGGTAGCGCAGGTGATTGCACTCATGACATCTCGCTAACCCATTCAGTTTGAGTTTTGTAACTTAAGT
AGAATCGATTTGGAGATGTT

Downstream 100 bases:

>100_bases
TTGACCTTGCTGGCGTCTGCCACGCCAGCTTTCTGAATTCACTATGGCGCTCGTCTTACTCACTGTGCAGTGCACAGAGA
GCGCCTTTTTTCGCCTTGGA

Product: lysine/cadaverine antiporter

Products: cadaverine [Cytoplasm]; L-lysine [Cytoplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MSSNAKKIGLIACTGVVAGNMMGSGIALLPSTLASVGSISIYSWLICIVGALSLAFVFARLATKNPQEGGPIAYAGEVSP
VFGFQTGVLYYHANWIGNLAIAITGVSYLSVFFPVLNNPIPAGLATIASVWLFTLVNLLGGSWVSRLCTIGLVLILIPVV
GTALFGWTHFDSALYSQNWNVSAGSDGHAVITAVLICLWSFVGVESAAVSSGMVENPKRTVPLATMLGTGLAGLIYVLST
QMISGMFPASEVAASGAPFALATTALFGSWTAPFVSAFTALACFTSLGSWMMLVGEAGKRAANDGNFPKVFGETDRNGVP
KKGLLIASSMMTLLMLVLMFFSSETAHASDLFNQLTTDAVLLTMLPYFYSSINLIRFEGMTTRNTFVMLFSGIASLFCMV
ALAGAEGSTLTATFIMSLIILMFYSKKAGLDKYLETHPQQASAQF

Sequences:

>Translated_445_residues
MSSNAKKIGLIACTGVVAGNMMGSGIALLPSTLASVGSISIYSWLICIVGALSLAFVFARLATKNPQEGGPIAYAGEVSP
VFGFQTGVLYYHANWIGNLAIAITGVSYLSVFFPVLNNPIPAGLATIASVWLFTLVNLLGGSWVSRLCTIGLVLILIPVV
GTALFGWTHFDSALYSQNWNVSAGSDGHAVITAVLICLWSFVGVESAAVSSGMVENPKRTVPLATMLGTGLAGLIYVLST
QMISGMFPASEVAASGAPFALATTALFGSWTAPFVSAFTALACFTSLGSWMMLVGEAGKRAANDGNFPKVFGETDRNGVP
KKGLLIASSMMTLLMLVLMFFSSETAHASDLFNQLTTDAVLLTMLPYFYSSINLIRFEGMTTRNTFVMLFSGIASLFCMV
ALAGAEGSTLTATFIMSLIILMFYSKKAGLDKYLETHPQQASAQF
>Mature_444_residues
SSNAKKIGLIACTGVVAGNMMGSGIALLPSTLASVGSISIYSWLICIVGALSLAFVFARLATKNPQEGGPIAYAGEVSPV
FGFQTGVLYYHANWIGNLAIAITGVSYLSVFFPVLNNPIPAGLATIASVWLFTLVNLLGGSWVSRLCTIGLVLILIPVVG
TALFGWTHFDSALYSQNWNVSAGSDGHAVITAVLICLWSFVGVESAAVSSGMVENPKRTVPLATMLGTGLAGLIYVLSTQ
MISGMFPASEVAASGAPFALATTALFGSWTAPFVSAFTALACFTSLGSWMMLVGEAGKRAANDGNFPKVFGETDRNGVPK
KGLLIASSMMTLLMLVLMFFSSETAHASDLFNQLTTDAVLLTMLPYFYSSINLIRFEGMTTRNTFVMLFSGIASLFCMVA
LAGAEGSTLTATFIMSLIILMFYSKKAGLDKYLETHPQQASAQF

Specific function: Probable cadaverine/lysine antiporter or part of it [H]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Homo sapiens, GI115648063, Length=362, Percent_Identity=27.3480662983425, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI115648022, Length=362, Percent_Identity=27.3480662983425, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1790575, Length=425, Percent_Identity=68.9411764705882, Blast_Score=595, Evalue=1e-171,
Organism=Escherichia coli, GI2367353, Length=428, Percent_Identity=39.2523364485981, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI1786908, Length=381, Percent_Identity=33.5958005249344, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1787890, Length=380, Percent_Identity=27.3684210526316, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87082250, Length=345, Percent_Identity=25.2173913043478, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI48994972, Length=390, Percent_Identity=23.8461538461538, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI221331183, Length=301, Percent_Identity=27.5747508305648, Blast_Score=65, Evalue=7e-11,
Organism=Drosophila melanogaster, GI17647653, Length=301, Percent_Identity=27.5747508305648, Blast_Score=65, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24664379, Length=301, Percent_Identity=27.5747508305648, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004754 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 46908; Mature: 46777

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSNAKKIGLIACTGVVAGNMMGSGIALLPSTLASVGSISIYSWLICIVGALSLAFVFAR
CCCCCCEEEEEEEHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
LATKNPQEGGPIAYAGEVSPVFGFQTGVLYYHANWIGNLAIAITGVSYLSVFFPVLNNPI
HHCCCCCCCCCEEEECCCCCHHCCHHCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PAGLATIASVWLFTLVNLLGGSWVSRLCTIGLVLILIPVVGTALFGWTHFDSALYSQNWN
CHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VSAGSDGHAVITAVLICLWSFVGVESAAVSSGMVENPKRTVPLATMLGTGLAGLIYVLST
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
QMISGMFPASEVAASGAPFALATTALFGSWTAPFVSAFTALACFTSLGSWMMLVGEAGKR
HHHHCCCCHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AANDGNFPKVFGETDRNGVPKKGLLIASSMMTLLMLVLMFFSSETAHASDLFNQLTTDAV
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LLTMLPYFYSSINLIRFEGMTTRNTFVMLFSGIASLFCMVALAGAEGSTLTATFIMSLII
HHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LMFYSKKAGLDKYLETHPQQASAQF
HHHHHCCCCHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SSNAKKIGLIACTGVVAGNMMGSGIALLPSTLASVGSISIYSWLICIVGALSLAFVFAR
CCCCCEEEEEEEHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
LATKNPQEGGPIAYAGEVSPVFGFQTGVLYYHANWIGNLAIAITGVSYLSVFFPVLNNPI
HHCCCCCCCCCEEEECCCCCHHCCHHCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PAGLATIASVWLFTLVNLLGGSWVSRLCTIGLVLILIPVVGTALFGWTHFDSALYSQNWN
CHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VSAGSDGHAVITAVLICLWSFVGVESAAVSSGMVENPKRTVPLATMLGTGLAGLIYVLST
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
QMISGMFPASEVAASGAPFALATTALFGSWTAPFVSAFTALACFTSLGSWMMLVGEAGKR
HHHHCCCCHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AANDGNFPKVFGETDRNGVPKKGLLIASSMMTLLMLVLMFFSSETAHASDLFNQLTTDAV
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LLTMLPYFYSSINLIRFEGMTTRNTFVMLFSGIASLFCMVALAGAEGSTLTATFIMSLII
HHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LMFYSKKAGLDKYLETHPQQASAQF
HHHHHCCCCHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: cadaverine [Cytoplasm]; Proton [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: cadaverine [Cytoplasm] + Proton [Periplasm] + L-lysine [Periplasm] = cadaverine [Cytoplasm] + L-lysine [Cytoplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]