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The map label for this gene is mscM [H]

Identifier: 227080480

GI number: 227080480

Start: 255424

End: 256662

Strand: Reverse

Name: mscM [H]

Synonym: VCM66_0250

Alternate gene names: 227080480

Gene position: 256662-255424 (Counterclockwise)

Preceding gene: 227080481

Following gene: 227080476

Centisome position: 8.87

GC content: 49.48

Gene sequence:

>1239_bases
ATGCTTGAAACATTAACCTTGTGGTTACAGAAGTTTGATATTGAAATTGAACGCGTGATCCAGCTTGCGGAAGCGCTCGG
GCTGATCGCAGCCATTTCGATTGCCATTCATCTCGTGTTACACCGTGGAGTGCTGCTGTGGCTCAAACGCCATACCCACC
ATGAGCAAAGCGTTTGGCGCAATGCTCTGTTTAAAGAGCACCTATTTAGCCGCGTTGCCTTACTGATCCAAGGTGTGGTG
ATTGCGGTGCAAACCCAGTTGTGGCTAAGCCCTAACACCTTTGCTTACGATGTTCTGCACACCCTAACCTCATTGTGGAT
TGTGGTGTTTGGCATGCTGGCCACTTACTCCGTACTCAACGTGCTGGAACTGCTGATCAGCCGAACCCAAGTGGGTAAAA
ACCTGCCGACACGCGGCATTTTGCAAAGCATCAAGCTGATTGTGTTTATTATCGCGGCGCTGTTTTTCACCTCAATTTTG
ATCGGTAAATCGCCAGTGATTCTGCTCAGCGGCTTAGGTGCGATGACCGCCGTCTTTATGTTGGTATTTAAAGACCCGAT
CCTCGGCTTAGTTGCAGGGGTACAACTTTCGGCCAACAAAATGCTCTCGGTCGGTGACTGGTTGGAAATGCCCAAATATG
GCGCAGATGGCTCTGTGATTGATATCAGCCTAACCACAGTCAAAGTGCAGAACTGGGATAAAACCATCACCACCATTCCA
AGCTACGCCCTGATTTCAGATTCCTTTAAAAACTGGAAAGGCATGCAAGAGTCTGGCGGTCGCCGTATCAAACGCAGCAT
TTTGATTGATGCCACCAGCGTGCATTTTTTAACAGAAGAAGAGAAACAAGCGCTGAAAAAAGCGCAACTGCTTGAGCCCT
ACTTGGTCGAGAAAGAGCAAGAGATCAGCAGCTACAACCAACAAAAACATTGGGATTTGGCCTGCCGTATCAATGGCCGC
CGCCTGACCAACATCGGCAGTTTCCGCGCTTATTTAGAGCGCTACCTGCGTACTCACCCCAATATTCACCAAGATATGAC
GCTGATGGTGCGTCAGTTAGCGCCCACTCACGATGGCATTTCGCTAGAGGTGTACTGCTTTACCAGCACCACAGTGTGGG
TGGAGTATGAGCGTATTCAGTCGGATATTTTTGATCATATTTACGCGGTACTGCCTGAGTTTGACCTGCGCGTCTCGCAA
GCCCCCACAGGCAATGACTTTCGCGCTCTGAGAGGCTAA

Upstream 100 bases:

>100_bases
CATCGTGCCCTACCTACGCCAACGAGTGAAGCAAGTACTTACATAGCAAGCCGTTAGGGTTTGAGGTAGTATGGCGCGCA
GATTGATTAAGGAGTAAGCC

Downstream 100 bases:

>100_bases
ACACCGCCCCTCCATTCGCGCGCATCGATCTTACCCCTCCTCCTTCTAGGGGGGAGGACGGGAGGGGGTATTTCACACCA
AGCCAAACAGAAAATGCACA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MLETLTLWLQKFDIEIERVIQLAEALGLIAAISIAIHLVLHRGVLLWLKRHTHHEQSVWRNALFKEHLFSRVALLIQGVV
IAVQTQLWLSPNTFAYDVLHTLTSLWIVVFGMLATYSVLNVLELLISRTQVGKNLPTRGILQSIKLIVFIIAALFFTSIL
IGKSPVILLSGLGAMTAVFMLVFKDPILGLVAGVQLSANKMLSVGDWLEMPKYGADGSVIDISLTTVKVQNWDKTITTIP
SYALISDSFKNWKGMQESGGRRIKRSILIDATSVHFLTEEEKQALKKAQLLEPYLVEKEQEISSYNQQKHWDLACRINGR
RLTNIGSFRAYLERYLRTHPNIHQDMTLMVRQLAPTHDGISLEVYCFTSTTVWVEYERIQSDIFDHIYAVLPEFDLRVSQ
APTGNDFRALRG

Sequences:

>Translated_412_residues
MLETLTLWLQKFDIEIERVIQLAEALGLIAAISIAIHLVLHRGVLLWLKRHTHHEQSVWRNALFKEHLFSRVALLIQGVV
IAVQTQLWLSPNTFAYDVLHTLTSLWIVVFGMLATYSVLNVLELLISRTQVGKNLPTRGILQSIKLIVFIIAALFFTSIL
IGKSPVILLSGLGAMTAVFMLVFKDPILGLVAGVQLSANKMLSVGDWLEMPKYGADGSVIDISLTTVKVQNWDKTITTIP
SYALISDSFKNWKGMQESGGRRIKRSILIDATSVHFLTEEEKQALKKAQLLEPYLVEKEQEISSYNQQKHWDLACRINGR
RLTNIGSFRAYLERYLRTHPNIHQDMTLMVRQLAPTHDGISLEVYCFTSTTVWVEYERIQSDIFDHIYAVLPEFDLRVSQ
APTGNDFRALRG
>Mature_412_residues
MLETLTLWLQKFDIEIERVIQLAEALGLIAAISIAIHLVLHRGVLLWLKRHTHHEQSVWRNALFKEHLFSRVALLIQGVV
IAVQTQLWLSPNTFAYDVLHTLTSLWIVVFGMLATYSVLNVLELLISRTQVGKNLPTRGILQSIKLIVFIIAALFFTSIL
IGKSPVILLSGLGAMTAVFMLVFKDPILGLVAGVQLSANKMLSVGDWLEMPKYGADGSVIDISLTTVKVQNWDKTITTIP
SYALISDSFKNWKGMQESGGRRIKRSILIDATSVHFLTEEEKQALKKAQLLEPYLVEKEQEISSYNQQKHWDLACRINGR
RLTNIGSFRAYLERYLRTHPNIHQDMTLMVRQLAPTHDGISLEVYCFTSTTVWVEYERIQSDIFDHIYAVLPEFDLRVSQ
APTGNDFRALRG

Specific function: Mechanosensitive channel of miniconductance that confers protection against mild hypoosmotic shock. Overexpression confers protection against severe shocks [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786791, Length=399, Percent_Identity=54.3859649122807, Blast_Score=455, Evalue=1e-129,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 46767; Mature: 46767

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLETLTLWLQKFDIEIERVIQLAEALGLIAAISIAIHLVLHRGVLLWLKRHTHHEQSVWR
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
NALFKEHLFSRVALLIQGVVIAVQTQLWLSPNTFAYDVLHTLTSLWIVVFGMLATYSVLN
HHHHHHHHHHHHHHHHHHHHHEEEHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLELLISRTQVGKNLPTRGILQSIKLIVFIIAALFFTSILIGKSPVILLSGLGAMTAVFM
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCHHHHHHHHH
LVFKDPILGLVAGVQLSANKMLSVGDWLEMPKYGADGSVIDISLTTVKVQNWDKTITTIP
HHHHHHHHHHHHCCEECCCCCCCCHHHHHCCCCCCCCCEEEEEEEEEEECCCCCHHHHCC
SYALISDSFKNWKGMQESGGRRIKRSILIDATSVHFLTEEEKQALKKAQLLEPYLVEKEQ
CHHHHHHHHHHCCCCHHCCCHHHHHHHEEEEHHHHEECHHHHHHHHHHHHCCHHHHCHHH
EISSYNQQKHWDLACRINGRRLTNIGSFRAYLERYLRTHPNIHQDMTLMVRQLAPTHDGI
HHHHCCCCCCCEEEEEECCCEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC
SLEVYCFTSTTVWVEYERIQSDIFDHIYAVLPEFDLRVSQAPTGNDFRALRG
EEEEEEEECEEEEEEHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCC
>Mature Secondary Structure
MLETLTLWLQKFDIEIERVIQLAEALGLIAAISIAIHLVLHRGVLLWLKRHTHHEQSVWR
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
NALFKEHLFSRVALLIQGVVIAVQTQLWLSPNTFAYDVLHTLTSLWIVVFGMLATYSVLN
HHHHHHHHHHHHHHHHHHHHHEEEHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLELLISRTQVGKNLPTRGILQSIKLIVFIIAALFFTSILIGKSPVILLSGLGAMTAVFM
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCHHHHHHHHH
LVFKDPILGLVAGVQLSANKMLSVGDWLEMPKYGADGSVIDISLTTVKVQNWDKTITTIP
HHHHHHHHHHHHCCEECCCCCCCCHHHHHCCCCCCCCCEEEEEEEEEEECCCCCHHHHCC
SYALISDSFKNWKGMQESGGRRIKRSILIDATSVHFLTEEEKQALKKAQLLEPYLVEKEQ
CHHHHHHHHHHCCCCHHCCCHHHHHHHEEEEHHHHEECHHHHHHHHHHHHCCHHHHCHHH
EISSYNQQKHWDLACRINGRRLTNIGSFRAYLERYLRTHPNIHQDMTLMVRQLAPTHDGI
HHHHCCCCCCCEEEEEECCCEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC
SLEVYCFTSTTVWVEYERIQSDIFDHIYAVLPEFDLRVSQAPTGNDFRALRG
EEEEEEEECEEEEEEHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 7813889 [H]