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The map label for this gene is rfbI [H]

Identifier: 227080466

GI number: 227080466

Start: 239639

End: 240454

Strand: Direct

Name: rfbI [H]

Synonym: VCM66_0235

Alternate gene names: 227080466

Gene position: 239639-240454 (Clockwise)

Preceding gene: 227080465

Following gene: 227080467

Centisome position: 8.28

GC content: 36.4

Gene sequence:

>816_bases
TTGGTATGGTCAGCTACTTCTCGTACAAAGTACTCGATAAAAAAGTCATTTTTTGGGTGTAGTATGATAGAGCTATCTAA
TGTTAATTTGCACTACCCTGTGCCAGGGCATTTCTCACATTCTTTGCAAACAACAATATCCAGCAAAATTGGTGGTGTAC
TGGGTTCTAGTTCTGCAAAAGATAAAGAAATGAAGTATGTTCATGCACTAAGAGATATTAATCTAAAGCTAGAAGATAGC
AGTAGGTTGGGTATTATTGGGCATAATGGTGCTGGCAAAACGACTCTACTTAGGTTACTTTCTCAGGTTTATCCACCAAC
GTCAGGAAAGGTTACTATCGAAGGAAAGATTTCAGCATTGACTGACTTCACTCTTGGAATGGATCCCAATGCAACAGGTC
TAAAGAATATCGAATTTAGATTAGTATTTATGGGATGTACATTTAAGGAAGCGCAACAAGCAGTTGAAGAAATCGTTGCT
TTTTCAGAACTCGGCGAATTTATCAATCTACCAGTCAGAACTTATTCTACCGGTATGTTCTTACGTTTGGCTTTTGCAAT
TTCTACACATTTCACTCCGGATATATTGATTTTAGATGAAGTTATTGGGGCTGGTGATGAAACTTTCCGTGAGAAAGCAT
TATCACGTTTGGAAAGTCTGATCAAGAAGTCAAGAATGGTTGTTTTGTCTTCTCATGATTTAAATGCTATTAAACAATAT
TGTGATCAGGCTATTGTAATGGAGAAAGGTGAAATTGTTTTCAATGGAACACCACAATCATGCATTGACTACTATTTAAA
CAGTGTCAAAAAATAA

Upstream 100 bases:

>100_bases
AATCCATTTGCTTCGCTATTGGAATTAATTAGAAATCCGATAATAGGAATCGACGTTAATCCTTTAGCATTTGTTTCGTT
AGTAGCATGGACTTTTATAA

Downstream 100 bases:

>100_bases
TCAACTAGATAAAAAATACAATCTTTTAACTATATTGGATGGTTTATATGTCTATCTCTGAAGAAAAAATTATCAATCTT
ATTGCAGGAATTCTTGAAGT

Product: lipopolysaccharide/O-antigen transport protein

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MVWSATSRTKYSIKKSFFGCSMIELSNVNLHYPVPGHFSHSLQTTISSKIGGVLGSSSAKDKEMKYVHALRDINLKLEDS
SRLGIIGHNGAGKTTLLRLLSQVYPPTSGKVTIEGKISALTDFTLGMDPNATGLKNIEFRLVFMGCTFKEAQQAVEEIVA
FSELGEFINLPVRTYSTGMFLRLAFAISTHFTPDILILDEVIGAGDETFREKALSRLESLIKKSRMVVLSSHDLNAIKQY
CDQAIVMEKGEIVFNGTPQSCIDYYLNSVKK

Sequences:

>Translated_271_residues
MVWSATSRTKYSIKKSFFGCSMIELSNVNLHYPVPGHFSHSLQTTISSKIGGVLGSSSAKDKEMKYVHALRDINLKLEDS
SRLGIIGHNGAGKTTLLRLLSQVYPPTSGKVTIEGKISALTDFTLGMDPNATGLKNIEFRLVFMGCTFKEAQQAVEEIVA
FSELGEFINLPVRTYSTGMFLRLAFAISTHFTPDILILDEVIGAGDETFREKALSRLESLIKKSRMVVLSSHDLNAIKQY
CDQAIVMEKGEIVFNGTPQSCIDYYLNSVKK
>Mature_271_residues
MVWSATSRTKYSIKKSFFGCSMIELSNVNLHYPVPGHFSHSLQTTISSKIGGVLGSSSAKDKEMKYVHALRDINLKLEDS
SRLGIIGHNGAGKTTLLRLLSQVYPPTSGKVTIEGKISALTDFTLGMDPNATGLKNIEFRLVFMGCTFKEAQQAVEEIVA
FSELGEFINLPVRTYSTGMFLRLAFAISTHFTPDILILDEVIGAGDETFREKALSRLESLIKKSRMVVLSSHDLNAIKQY
CDQAIVMEKGEIVFNGTPQSCIDYYLNSVKK

Specific function: May form an ATP-driven O-antigen export apparatus, in association with RfbA [H]

COG id: COG1134

COG function: function code GM; ABC-type polysaccharide/polyol phosphate transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI27436953, Length=199, Percent_Identity=28.1407035175879, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI116734710, Length=200, Percent_Identity=31, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI153792144, Length=205, Percent_Identity=25.3658536585366, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI6005701, Length=199, Percent_Identity=25.1256281407035, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI27477115, Length=199, Percent_Identity=24.6231155778894, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI105990541, Length=220, Percent_Identity=26.8181818181818, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI45446740, Length=201, Percent_Identity=30.3482587064677, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI47078218, Length=201, Percent_Identity=30.3482587064677, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI31657092, Length=204, Percent_Identity=26.4705882352941, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1789032, Length=217, Percent_Identity=28.110599078341, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787758, Length=209, Percent_Identity=27.2727272727273, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87081782, Length=188, Percent_Identity=25.531914893617, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87081791, Length=208, Percent_Identity=25.4807692307692, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787029, Length=210, Percent_Identity=27.1428571428571, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788225, Length=215, Percent_Identity=27.4418604651163, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1789962, Length=219, Percent_Identity=26.027397260274, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI87081709, Length=200, Percent_Identity=26, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786319, Length=207, Percent_Identity=28.5024154589372, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788506, Length=223, Percent_Identity=26.9058295964126, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786398, Length=209, Percent_Identity=24.4019138755981, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1787143, Length=217, Percent_Identity=27.1889400921659, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI48994943, Length=219, Percent_Identity=22.8310502283105, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787370, Length=199, Percent_Identity=25.6281407035176, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1787547, Length=222, Percent_Identity=22.5225225225225, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786654, Length=214, Percent_Identity=27.5700934579439, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI115533608, Length=193, Percent_Identity=30.0518134715026, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI221500365, Length=192, Percent_Identity=25.5208333333333, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI116007184, Length=192, Percent_Identity=25.5208333333333, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI28573571, Length=200, Percent_Identity=24.5, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28573573, Length=200, Percent_Identity=24.5, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI85726357, Length=237, Percent_Identity=25.3164556962025, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 29960; Mature: 29960

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVWSATSRTKYSIKKSFFGCSMIELSNVNLHYPVPGHFSHSLQTTISSKIGGVLGSSSAK
CCCCCCCCCHHHHHHHHCCCEEEEEECCEEECCCCCCCCHHHHHHHHHHHCCHHCCCCCC
DKEMKYVHALRDINLKLEDSSRLGIIGHNGAGKTTLLRLLSQVYPPTSGKVTIEGKISAL
HHHHHHHHHHHHCCEEEECCCEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEEEEEEE
TDFTLGMDPNATGLKNIEFRLVFMGCTFKEAQQAVEEIVAFSELGEFINLPVRTYSTGMF
EEEEECCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHH
LRLAFAISTHFTPDILILDEVIGAGDETFREKALSRLESLIKKSRMVVLSSHDLNAIKQY
HHHHHHHHHCCCCCEEEHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHH
CDQAIVMEKGEIVFNGTPQSCIDYYLNSVKK
HHHHHEEECCCEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MVWSATSRTKYSIKKSFFGCSMIELSNVNLHYPVPGHFSHSLQTTISSKIGGVLGSSSAK
CCCCCCCCCHHHHHHHHCCCEEEEEECCEEECCCCCCCCHHHHHHHHHHHCCHHCCCCCC
DKEMKYVHALRDINLKLEDSSRLGIIGHNGAGKTTLLRLLSQVYPPTSGKVTIEGKISAL
HHHHHHHHHHHHCCEEEECCCEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEEEEEEE
TDFTLGMDPNATGLKNIEFRLVFMGCTFKEAQQAVEEIVAFSELGEFINLPVRTYSTGMF
EEEEECCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHH
LRLAFAISTHFTPDILILDEVIGAGDETFREKALSRLESLIKKSRMVVLSSHDLNAIKQY
HHHHHHHHHCCCCCEEEHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHH
CDQAIVMEKGEIVFNGTPQSCIDYYLNSVKK
HHHHHEEECCCEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8626291 [H]