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The map label for this gene is rfaF [H]

Identifier: 227080456

GI number: 227080456

Start: 226390

End: 227430

Strand: Reverse

Name: rfaF [H]

Synonym: VCM66_0224

Alternate gene names: 227080456

Gene position: 227430-226390 (Counterclockwise)

Preceding gene: 227080457

Following gene: 227080455

Centisome position: 7.86

GC content: 46.3

Gene sequence:

>1041_bases
TTGATGAAAATTCTTGTTATTGGCCCTTCTTGGGTTGGCGATATGGTCATGTCACAAAGCTTGTACCAGCGCCTAAAACA
ACAGCATCCTGATGCACAAATTGACGTTTTAGCTCCTGCTTGGTGTAAGCCCATTTTGGAACGCATGCCTGAGGTCAATC
AAGCGATTGAAATGACCATAGGTCACGGTGCTTTCAATCTGCTTGGGCGACGTGCGATTGGGTGTGAGCTGCGCGATAAT
CGGTATACCCATGCCATTGTGCTGCCTAATTCAGCTAAATCAGCCTTGATCCCTTGGTTTGCTAACATTCCAAAACGTAC
CGGCTGGAAAGGGGAATTTCGCTACGGCTTACTCAATGATTTGCGCCCAGATAAGCGTGTTTTCCAATATATGGTGGAAA
GGTATGTTGCTTTGGCTCACCCAAAAGCCACAATGCTTGCCGATGTTTCGTTAGAACATTGCCCAAGACCTAAACTCGTC
ATCGACGCCATCGTGCAGCAAGCCGCTCGACAGCGACTCAGCTTAGTCTCATCACGTCCCGTCATTGGACTCTGTCCTGG
TGCAGAATTTGGCCCCGCGAAACGCTGGCCTGATCATTACTACGCTGAAGTCGCTCGTTATGCTATTGAGCAAGGTTTTC
AAGTATGGCTTTTTGGCTCAGCAAAAGATCACTCCGTCACCACCCAAATTCAACAGGCTTTAAGTGAAGAACAGCGGGAA
TACTGCGCCAATCTCGCCGGAGAAACCTCCCTCATCGAAGCCGTTGATCTTCTTGCAGCTTGCCATACTGTGGTCAGTAA
CGATTCTGGTTTAATGCACGTTTCAGCCGCTGTTGGTTGTAACATTGTGGCAATTTATGGTTCAAGCTCTCCCAAATATA
CACCACCACTGACGGATAAACTGGCAGTCGTACATACAGAGATCGAATGTCGTCCGTGCTTTAAGCGAGTATGTCCGTTA
GAACATTTAAATTGCTTAAATCAACTAAAACCTGCACAAGTCATTAAAGCTTTAGATAAATTTATTGGAAAAGATTCATG
A

Upstream 100 bases:

>100_bases
GTTTAACTGAAGCACCACTACAAGCCAACCTTATTGGAACTTTTTATGGCTTTATGGTGGCAATTTTTTATGCTTACATC
GCAGCCAAAAGAGCAAAAAA

Downstream 100 bases:

>100_bases
AACACCTCTGGTTTGAAAAAGGTGCTTATGCTTCGAAAGCGGCACGCCAATACTTAAATGACCATTTTAACCCTCTAGCA
GTTAAGAAAACCGCTGTCAT

Product: ADP-heptose--LPS heptosyltransferase II

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MMKILVIGPSWVGDMVMSQSLYQRLKQQHPDAQIDVLAPAWCKPILERMPEVNQAIEMTIGHGAFNLLGRRAIGCELRDN
RYTHAIVLPNSAKSALIPWFANIPKRTGWKGEFRYGLLNDLRPDKRVFQYMVERYVALAHPKATMLADVSLEHCPRPKLV
IDAIVQQAARQRLSLVSSRPVIGLCPGAEFGPAKRWPDHYYAEVARYAIEQGFQVWLFGSAKDHSVTTQIQQALSEEQRE
YCANLAGETSLIEAVDLLAACHTVVSNDSGLMHVSAAVGCNIVAIYGSSSPKYTPPLTDKLAVVHTEIECRPCFKRVCPL
EHLNCLNQLKPAQVIKALDKFIGKDS

Sequences:

>Translated_346_residues
MMKILVIGPSWVGDMVMSQSLYQRLKQQHPDAQIDVLAPAWCKPILERMPEVNQAIEMTIGHGAFNLLGRRAIGCELRDN
RYTHAIVLPNSAKSALIPWFANIPKRTGWKGEFRYGLLNDLRPDKRVFQYMVERYVALAHPKATMLADVSLEHCPRPKLV
IDAIVQQAARQRLSLVSSRPVIGLCPGAEFGPAKRWPDHYYAEVARYAIEQGFQVWLFGSAKDHSVTTQIQQALSEEQRE
YCANLAGETSLIEAVDLLAACHTVVSNDSGLMHVSAAVGCNIVAIYGSSSPKYTPPLTDKLAVVHTEIECRPCFKRVCPL
EHLNCLNQLKPAQVIKALDKFIGKDS
>Mature_346_residues
MMKILVIGPSWVGDMVMSQSLYQRLKQQHPDAQIDVLAPAWCKPILERMPEVNQAIEMTIGHGAFNLLGRRAIGCELRDN
RYTHAIVLPNSAKSALIPWFANIPKRTGWKGEFRYGLLNDLRPDKRVFQYMVERYVALAHPKATMLADVSLEHCPRPKLV
IDAIVQQAARQRLSLVSSRPVIGLCPGAEFGPAKRWPDHYYAEVARYAIEQGFQVWLFGSAKDHSVTTQIQQALSEEQRE
YCANLAGETSLIEAVDLLAACHTVVSNDSGLMHVSAAVGCNIVAIYGSSSPKYTPPLTDKLAVVHTEIECRPCFKRVCPL
EHLNCLNQLKPAQVIKALDKFIGKDS

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=346, Percent_Identity=52.6011560693642, Blast_Score=357, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.4.1.-

Molecular weight: Translated: 38584; Mature: 38584

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKILVIGPSWVGDMVMSQSLYQRLKQQHPDAQIDVLAPAWCKPILERMPEVNQAIEMTI
CEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHCCHHHHHHHHHC
GHGAFNLLGRRAIGCELRDNRYTHAIVLPNSAKSALIPWFANIPKRTGWKGEFRYGLLND
CCHHHHHHHHHHCCCEECCCCEEEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHC
LRPDKRVFQYMVERYVALAHPKATMLADVSLEHCPRPKLVIDAIVQQAARQRLSLVSSRP
CCHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
VIGLCPGAEFGPAKRWPDHYYAEVARYAIEQGFQVWLFGSAKDHSVTTQIQQALSEEQRE
EEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
YCANLAGETSLIEAVDLLAACHTVVSNDSGLMHVSAAVGCNIVAIYGSSSPKYTPPLTDK
HHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEEECCCCCCCCCCCCC
LAVVHTEIECRPCFKRVCPLEHLNCLNQLKPAQVIKALDKFIGKDS
EEEEEECCCCHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMKILVIGPSWVGDMVMSQSLYQRLKQQHPDAQIDVLAPAWCKPILERMPEVNQAIEMTI
CEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHCCHHHHHHHHHC
GHGAFNLLGRRAIGCELRDNRYTHAIVLPNSAKSALIPWFANIPKRTGWKGEFRYGLLND
CCHHHHHHHHHHCCCEECCCCEEEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHC
LRPDKRVFQYMVERYVALAHPKATMLADVSLEHCPRPKLVIDAIVQQAARQRLSLVSSRP
CCHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
VIGLCPGAEFGPAKRWPDHYYAEVARYAIEQGFQVWLFGSAKDHSVTTQIQQALSEEQRE
EEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
YCANLAGETSLIEAVDLLAACHTVVSNDSGLMHVSAAVGCNIVAIYGSSSPKYTPPLTDK
HHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEEECCCCCCCCCCCCC
LAVVHTEIECRPCFKRVCPLEHLNCLNQLKPAQVIKALDKFIGKDS
EEEEEECCCCHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 2198271; 8478319 [H]