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The map label for this gene is kdtA [H]

Identifier: 227080453

GI number: 227080453

Start: 223279

End: 224598

Strand: Reverse

Name: kdtA [H]

Synonym: VCM66_0221

Alternate gene names: 227080453

Gene position: 224598-223279 (Counterclockwise)

Preceding gene: 227080454

Following gene: 227080452

Centisome position: 7.76

GC content: 47.5

Gene sequence:

>1320_bases
ATGGACACACATCAATCCCGAAGATCTCGTGAAAAAATAATGCTGCTCCGTACGCTTTATACGCTTTTACTCACTTTAGC
TGCACCGTTTTTACTGTATGGCCTCTATCGCCGTAGACAGGGGAAACCCAGTGTCGGAAAACGTTGGAAAGAGCATTTCG
GCATTACCCCACCACTAAAAACCACAACCCCACCGATCTGGATCCATGCCGCGTCGGTGGGTGAAACCCTAGCGGTAACG
CCACTCATAAAACAAATTAAGCAACGTTCACCGAATACACCAATTCTGCTGACTACCACGACCCCGACGGGTGCTGAACA
AGCGGAAAAATTGGCGGATTGGGTTGAGCATCGTTATACCCCGATTGATTTTTCCTTTGCGGTGAGAGGCTTTTTGCGTC
GTGTACGACCTTGCCAATTGATCATAGTGGAAACAGAACTGTGGCCAAATACTCTGCATACCGTTGCCAAAGCGGGTTTG
CCGATTACACTCGTGAATGCACGACTTTCGGAAAAATCCTATCGCGGCTATCAGCGTATTCACCCGTTCTTTAACAGTAT
GGCGAAACCTCTCAGCCTAGTGTTATGTCAATTTGCAGACGATGCGCAGCGTTTTATTCAGCTCGGTGTGGCTGAGACAA
AAATCAAAATTACCGGTTCGATTAAGTTTGATATCAACATCACCTATGAGGTGATTGCTCAAGGTGAAGCGCTCAGAACG
GCTTTAGGTAAACATCGTCCAGTCTGGATAGCCGCCAGCACACATCAAGGTGAAGATGAAATTGTGCTTGCGGCACATCA
AGAAATCCTCAAGCAGCATCCTAATGCTTTGTTGATTTTAGTGCCGCGCCATCCTGAACGTTTTGCGGCGGTGCATAAGC
TCGCAGCTAGCCTTTTTTCAGTGCAAACTCGTAGCAGCCAACAAACCATAACCTCCGACACTCAAGTGTATCTCGGCGAT
ACCATGGGAGAGATGCTGGTCTTGCTCGGCGCTTCTGATGTGTGTTTTATGGGTGGCAGTTTAGTGGGCAAAAAAGTCGG
CGGGCATAATTTGCTTGAACCTGCTGCACTGGCAAAACCCATCGTTACAGGCCCTAGCTTTTACAACTTTACCGATATCA
CCCACGCACTCATCAATGCACATGCCTGTGTGATTGCTGACCAACCAGAAACCATTGCCAAGCAGGTGAACCATTGGTTT
TCTGATGCTCAAGAACGGCAGCAATGCGGAAAAAATGCCTTAGCGATTGTAATGCAAAATCGTGGAGCTCTCGAAAATAC
CTTGACCGAATTAGGTTTTACTAAGGAATTCGCCCAATGA

Upstream 100 bases:

>100_bases
CAGCTAAAGCGGTGATTCACACTCAAGACCGTAAATACGTATGTATTCAGGGCACCTTTACCAATGAACACTTCCAGTAC
ATTGATAAACCTCGTGGTAA

Downstream 100 bases:

>100_bases
AAAAATTGATCGTCGACCTCGACGGCACTCTCACCCAAGCTAATACTTCAGATTATCGTAACGTGCTACCTCGGCTTGAT
GTGATTGAGCAACTTCGCGA

Product: 3-deoxy-D-manno-octulosonic-acid transferase

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MDTHQSRRSREKIMLLRTLYTLLLTLAAPFLLYGLYRRRQGKPSVGKRWKEHFGITPPLKTTTPPIWIHAASVGETLAVT
PLIKQIKQRSPNTPILLTTTTPTGAEQAEKLADWVEHRYTPIDFSFAVRGFLRRVRPCQLIIVETELWPNTLHTVAKAGL
PITLVNARLSEKSYRGYQRIHPFFNSMAKPLSLVLCQFADDAQRFIQLGVAETKIKITGSIKFDINITYEVIAQGEALRT
ALGKHRPVWIAASTHQGEDEIVLAAHQEILKQHPNALLILVPRHPERFAAVHKLAASLFSVQTRSSQQTITSDTQVYLGD
TMGEMLVLLGASDVCFMGGSLVGKKVGGHNLLEPAALAKPIVTGPSFYNFTDITHALINAHACVIADQPETIAKQVNHWF
SDAQERQQCGKNALAIVMQNRGALENTLTELGFTKEFAQ

Sequences:

>Translated_439_residues
MDTHQSRRSREKIMLLRTLYTLLLTLAAPFLLYGLYRRRQGKPSVGKRWKEHFGITPPLKTTTPPIWIHAASVGETLAVT
PLIKQIKQRSPNTPILLTTTTPTGAEQAEKLADWVEHRYTPIDFSFAVRGFLRRVRPCQLIIVETELWPNTLHTVAKAGL
PITLVNARLSEKSYRGYQRIHPFFNSMAKPLSLVLCQFADDAQRFIQLGVAETKIKITGSIKFDINITYEVIAQGEALRT
ALGKHRPVWIAASTHQGEDEIVLAAHQEILKQHPNALLILVPRHPERFAAVHKLAASLFSVQTRSSQQTITSDTQVYLGD
TMGEMLVLLGASDVCFMGGSLVGKKVGGHNLLEPAALAKPIVTGPSFYNFTDITHALINAHACVIADQPETIAKQVNHWF
SDAQERQQCGKNALAIVMQNRGALENTLTELGFTKEFAQ
>Mature_439_residues
MDTHQSRRSREKIMLLRTLYTLLLTLAAPFLLYGLYRRRQGKPSVGKRWKEHFGITPPLKTTTPPIWIHAASVGETLAVT
PLIKQIKQRSPNTPILLTTTTPTGAEQAEKLADWVEHRYTPIDFSFAVRGFLRRVRPCQLIIVETELWPNTLHTVAKAGL
PITLVNARLSEKSYRGYQRIHPFFNSMAKPLSLVLCQFADDAQRFIQLGVAETKIKITGSIKFDINITYEVIAQGEALRT
ALGKHRPVWIAASTHQGEDEIVLAAHQEILKQHPNALLILVPRHPERFAAVHKLAASLFSVQTRSSQQTITSDTQVYLGD
TMGEMLVLLGASDVCFMGGSLVGKKVGGHNLLEPAALAKPIVTGPSFYNFTDITHALINAHACVIADQPETIAKQVNHWF
SDAQERQQCGKNALAIVMQNRGALENTLTELGFTKEFAQ

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=420, Percent_Identity=42.6190476190476, Blast_Score=338, Evalue=3e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 48874; Mature: 48874

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTHQSRRSREKIMLLRTLYTLLLTLAAPFLLYGLYRRRQGKPSVGKRWKEHFGITPPLK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCC
TTTPPIWIHAASVGETLAVTPLIKQIKQRSPNTPILLTTTTPTGAEQAEKLADWVEHRYT
CCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCC
PIDFSFAVRGFLRRVRPCQLIIVETELWPNTLHTVAKAGLPITLVNARLSEKSYRGYQRI
CCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHCCCCEEEEECHHHHHHHHHHHHH
HPFFNSMAKPLSLVLCQFADDAQRFIQLGVAETKIKITGSIKFDINITYEVIAQGEALRT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEEEEEEEEEEECCHHHHH
ALGKHRPVWIAASTHQGEDEIVLAAHQEILKQHPNALLILVPRHPERFAAVHKLAASLFS
HHCCCCCEEEEECCCCCCCCEEHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHH
VQTRSSQQTITSDTQVYLGDTMGEMLVLLGASDVCFMGGSLVGKKVGGHNLLEPAALAKP
HHCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCHHHCCCHHHHCCCCCCCCCCHHHHHCC
IVTGPSFYNFTDITHALINAHACVIADQPETIAKQVNHWFSDAQERQQCGKNALAIVMQN
CCCCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEC
RGALENTLTELGFTKEFAQ
CCCHHHHHHHHCCHHHHCC
>Mature Secondary Structure
MDTHQSRRSREKIMLLRTLYTLLLTLAAPFLLYGLYRRRQGKPSVGKRWKEHFGITPPLK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCC
TTTPPIWIHAASVGETLAVTPLIKQIKQRSPNTPILLTTTTPTGAEQAEKLADWVEHRYT
CCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCC
PIDFSFAVRGFLRRVRPCQLIIVETELWPNTLHTVAKAGLPITLVNARLSEKSYRGYQRI
CCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHCCCCEEEEECHHHHHHHHHHHHH
HPFFNSMAKPLSLVLCQFADDAQRFIQLGVAETKIKITGSIKFDINITYEVIAQGEALRT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEEEEEEEEEEECCHHHHH
ALGKHRPVWIAASTHQGEDEIVLAAHQEILKQHPNALLILVPRHPERFAAVHKLAASLFS
HHCCCCCEEEEECCCCCCCCEEHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHH
VQTRSSQQTITSDTQVYLGDTMGEMLVLLGASDVCFMGGSLVGKKVGGHNLLEPAALAKP
HHCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCHHHCCCHHHHCCCCCCCCCCHHHHHCC
IVTGPSFYNFTDITHALINAHACVIADQPETIAKQVNHWFSDAQERQQCGKNALAIVMQN
CCCCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEC
RGALENTLTELGFTKEFAQ
CCCHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]