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The map label for this gene is rep [H]

Identifier: 227080400

GI number: 227080400

Start: 164660

End: 166675

Strand: Direct

Name: rep [H]

Synonym: VCM66_0166

Alternate gene names: 227080400

Gene position: 164660-166675 (Clockwise)

Preceding gene: 227080395

Following gene: 227080403

Centisome position: 5.69

GC content: 48.76

Gene sequence:

>2016_bases
ATGAAGCTGAACCCAAGACAAGATGAAGCGGTCAAGTACGTCTCCGGACCCTGTCTGGTATTGGCGGGGGCGGGTTCGGG
TAAAACACGCGTCATCACCAATAAAATTGCCTATTTGGTTCAGCAATGTGGCTATAAAGCACGCAATATTGCTGCGGTTA
CGTTTACCAATAAAGCGGCGCGTGAGATGAAAGAGCGCGTAGCTCAAACACTCGGTAAAGGTGAGTCGCGCGGCTTAATG
GTGTCGACCTTCCATACTCTTGGCTTAAACATCATTCGCCGTGAGTTTAAAGCTTTGGGCCTCAAAGCGGGTTTTTCACT
GTTTGACGACCAAGATCAGCTCGCGTTACTTAAAGAGCTGACCGAAAAGCAGCTCGACGGCGATAAAGATCTGCTGCGCT
TGCTACTGAGCACCATTTCCAACTGGAAAAATGACATGCTGACTCCGCCGCAAGCCAAGGCAATGGCGAAAGGGGAACAG
CAGCTGCTTTTTGCACACTGCTTTGAGCTTTACCAAAAACAGATGCAGTCTTACAACGCACTCGATTTCGATGATTTGAT
ATTGCTGCCGGTGTTACTGCTACGCAGCAATGAAGAAGTACGCCAACGCTGGCAAAATCGGATTCGCTATCTGTTGGTGG
ACGAATATCAAGATACCAACACCAGCCAATATGAGTTGGTCAAACTCTTAGTGGGTGAGCGTGGCCGTTTAACTGTGGTG
GGAGATGATGACCAATCCATTTATTCATGGCGTGGTGCAAAACCACAAAACTTGGTGTTACTTGGGGAAGATTTTCCGAG
TTTAAAGCTGATTAAGCTCGAACAAAATTATCGCTCGACCAGCCGCATTTTGCGTGCGGCGAACATTTTAATTGCCAATA
ACCCCCACGTTTACCAAAAGGCGCTCTTTTCTGAGTTAGCGGAAGGGGAAAAGCTCAAGGTCATACTGGCCAATAATGAA
GATCATGAAGCAGAGCGAGTGATCGCAGAAATCATCGCCCATAAGTTTCTCAATCGAACCGAATACCGAGATTACGCGAT
TCTTTATCGCGGAAACCATCAATCGCGACTGATTGAAAAATCGCTGACTCAAAACCGTGTGCCGTACAAACTTTCCGGCG
GCACTTCGTTTTTTGCGCGCGCAGAGATCAAAGACATCATGGCTTACCTGCGAGTGCTGGTGAACCCTGATGATGACAAC
GCATTTTTACGCATTGTGAATACACCGAAGCGTGAAATCGGCCCAGCTACGCTCGAAAAGTTAGGCAGTTACGCCAATAT
GCGGGGCAAAAGCCTATTTACCGCCAGTTTTGAGCTCGGTTTAGAGCAGCATTTGAGCGGGCGAGGGCTTGAGAACTTAC
GCCGCTTCACTGAGTGGTTGGTGGCGATTGCCGATAATGCTGAGCGGGGTAACACGGTGGAAGCGGTGCGCGCATTAGTG
CGTGATATTCGTTACGAAGATTGGTTGTATGAGACTTCCGCCAGTCCGAAAGCGGCCGAAATGCGCATGAAAAACGTCTC
CGATCTCTATTCATGGATCGTGGCCGATTTGGAAGGCGATAATCCCGATCAGCAAGAGAAAACTCTGAAAGAGGTGGTGC
AGCGTTTAACTCTGCGCGACATGATGGAGCGTGGCGAAGAGAACGATGACAGTGATGCGGTACAGCTGATGACACTGCAC
GCCTCAAAGGGCTTAGAATTTCCGTACGTGTATTTGATTGGAGCTGAAGAGGGCATTTTGCCGCATCAAACCAGTATTGA
TGAAGAGAATGTCGAAGAAGAGCGCCGCTTAATGTACGTTGGGATCACCCGAGCACAACGCGAGCTAACGTTTATCGTGT
GTAAAGAGCGCCGTCAGTTTGGTGAACTGATCAAACCTTCGCAAAGCCGCTTTTTGGATGAGTTGCCCCAGGACGATATC
GAGTGGGAAGTAAAGAAAAAGCCCGTCACCCAGGAAGAGCGTATGGCCAAAGGCCAAGCGCATATCGCTAATTTAAGAGC
GATGTTTAAGAAGTAA

Upstream 100 bases:

>100_bases
TACACTTGTGCAGTAAATTTTTGTAAAGGCAGTTTCTTTGTTGGTAATCCTCGGTGAGCGGATTATTATTGGCGGCTAAA
TAAAGAGGATTGAGAGCGCT

Downstream 100 bases:

>100_bases
ATAAAGAAGGGCTTGTCAAAACAAGCCCTTCTTTATTATCAATTGATGGAATGGTAAGCCCGCTTATTACAGGCCTGCGA
TCATATGCTCAATCGCAGCA

Product: ATP-dependent DNA helicase Rep

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 671; Mature: 671

Protein sequence:

>671_residues
MKLNPRQDEAVKYVSGPCLVLAGAGSGKTRVITNKIAYLVQQCGYKARNIAAVTFTNKAAREMKERVAQTLGKGESRGLM
VSTFHTLGLNIIRREFKALGLKAGFSLFDDQDQLALLKELTEKQLDGDKDLLRLLLSTISNWKNDMLTPPQAKAMAKGEQ
QLLFAHCFELYQKQMQSYNALDFDDLILLPVLLLRSNEEVRQRWQNRIRYLLVDEYQDTNTSQYELVKLLVGERGRLTVV
GDDDQSIYSWRGAKPQNLVLLGEDFPSLKLIKLEQNYRSTSRILRAANILIANNPHVYQKALFSELAEGEKLKVILANNE
DHEAERVIAEIIAHKFLNRTEYRDYAILYRGNHQSRLIEKSLTQNRVPYKLSGGTSFFARAEIKDIMAYLRVLVNPDDDN
AFLRIVNTPKREIGPATLEKLGSYANMRGKSLFTASFELGLEQHLSGRGLENLRRFTEWLVAIADNAERGNTVEAVRALV
RDIRYEDWLYETSASPKAAEMRMKNVSDLYSWIVADLEGDNPDQQEKTLKEVVQRLTLRDMMERGEENDDSDAVQLMTLH
ASKGLEFPYVYLIGAEEGILPHQTSIDEENVEEERRLMYVGITRAQRELTFIVCKERRQFGELIKPSQSRFLDELPQDDI
EWEVKKKPVTQEERMAKGQAHIANLRAMFKK

Sequences:

>Translated_671_residues
MKLNPRQDEAVKYVSGPCLVLAGAGSGKTRVITNKIAYLVQQCGYKARNIAAVTFTNKAAREMKERVAQTLGKGESRGLM
VSTFHTLGLNIIRREFKALGLKAGFSLFDDQDQLALLKELTEKQLDGDKDLLRLLLSTISNWKNDMLTPPQAKAMAKGEQ
QLLFAHCFELYQKQMQSYNALDFDDLILLPVLLLRSNEEVRQRWQNRIRYLLVDEYQDTNTSQYELVKLLVGERGRLTVV
GDDDQSIYSWRGAKPQNLVLLGEDFPSLKLIKLEQNYRSTSRILRAANILIANNPHVYQKALFSELAEGEKLKVILANNE
DHEAERVIAEIIAHKFLNRTEYRDYAILYRGNHQSRLIEKSLTQNRVPYKLSGGTSFFARAEIKDIMAYLRVLVNPDDDN
AFLRIVNTPKREIGPATLEKLGSYANMRGKSLFTASFELGLEQHLSGRGLENLRRFTEWLVAIADNAERGNTVEAVRALV
RDIRYEDWLYETSASPKAAEMRMKNVSDLYSWIVADLEGDNPDQQEKTLKEVVQRLTLRDMMERGEENDDSDAVQLMTLH
ASKGLEFPYVYLIGAEEGILPHQTSIDEENVEEERRLMYVGITRAQRELTFIVCKERRQFGELIKPSQSRFLDELPQDDI
EWEVKKKPVTQEERMAKGQAHIANLRAMFKK
>Mature_671_residues
MKLNPRQDEAVKYVSGPCLVLAGAGSGKTRVITNKIAYLVQQCGYKARNIAAVTFTNKAAREMKERVAQTLGKGESRGLM
VSTFHTLGLNIIRREFKALGLKAGFSLFDDQDQLALLKELTEKQLDGDKDLLRLLLSTISNWKNDMLTPPQAKAMAKGEQ
QLLFAHCFELYQKQMQSYNALDFDDLILLPVLLLRSNEEVRQRWQNRIRYLLVDEYQDTNTSQYELVKLLVGERGRLTVV
GDDDQSIYSWRGAKPQNLVLLGEDFPSLKLIKLEQNYRSTSRILRAANILIANNPHVYQKALFSELAEGEKLKVILANNE
DHEAERVIAEIIAHKFLNRTEYRDYAILYRGNHQSRLIEKSLTQNRVPYKLSGGTSFFARAEIKDIMAYLRVLVNPDDDN
AFLRIVNTPKREIGPATLEKLGSYANMRGKSLFTASFELGLEQHLSGRGLENLRRFTEWLVAIADNAERGNTVEAVRALV
RDIRYEDWLYETSASPKAAEMRMKNVSDLYSWIVADLEGDNPDQQEKTLKEVVQRLTLRDMMERGEENDDSDAVQLMTLH
ASKGLEFPYVYLIGAEEGILPHQTSIDEENVEEERRLMYVGITRAQRELTFIVCKERRQFGELIKPSQSRFLDELPQDDI
EWEVKKKPVTQEERMAKGQAHIANLRAMFKK

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=669, Percent_Identity=71.898355754858, Blast_Score=993, Evalue=0.0,
Organism=Escherichia coli, GI2367296, Length=660, Percent_Identity=39.3939393939394, Blast_Score=410, Evalue=1e-115,
Organism=Escherichia coli, GI1787196, Length=353, Percent_Identity=29.1784702549575, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6322369, Length=703, Percent_Identity=27.4537695590327, Blast_Score=210, Evalue=7e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 76906; Mature: 76906

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNPRQDEAVKYVSGPCLVLAGAGSGKTRVITNKIAYLVQQCGYKARNIAAVTFTNKAA
CCCCCCCHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHCEEEEEECCHHH
REMKERVAQTLGKGESRGLMVSTFHTLGLNIIRREFKALGLKAGFSLFDDQDQLALLKEL
HHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
TEKQLDGDKDLLRLLLSTISNWKNDMLTPPQAKAMAKGEQQLLFAHCFELYQKQMQSYNA
HHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
LDFDDLILLPVLLLRSNEEVRQRWQNRIRYLLVDEYQDTNTSQYELVKLLVGERGRLTVV
CCHHHHHHHHHHHHCCCHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEE
GDDDQSIYSWRGAKPQNLVLLGEDFPSLKLIKLEQNYRSTSRILRAANILIANNPHVYQK
ECCCCHHHHCCCCCCCCEEEECCCCCCEEEEEECHHHHHHHHHHHHHHEEEECCCHHHHH
ALFSELAEGEKLKVILANNEDHEAERVIAEIIAHKFLNRTEYRDYAILYRGNHQSRLIEK
HHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHH
SLTQNRVPYKLSGGTSFFARAEIKDIMAYLRVLVNPDDDNAFLRIVNTPKREIGPATLEK
HHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHCCHHHHHH
LGSYANMRGKSLFTASFELGLEQHLSGRGLENLRRFTEWLVAIADNAERGNTVEAVRALV
HHHHHCCCCCEEEEHHHHHCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
RDIRYEDWLYETSASPKAAEMRMKNVSDLYSWIVADLEGDNPDQQEKTLKEVVQRLTLRD
HHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
MMERGEENDDSDAVQLMTLHASKGLEFPYVYLIGAEEGILPHQTSIDEENVEEERRLMYV
HHHCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHEEEEE
GITRAQRELTFIVCKERRQFGELIKPSQSRFLDELPQDDIEWEVKKKPVTQEERMAKGQA
EEHHHHHHEEEEEEHHHHHHHHHCCCCHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHH
HIANLRAMFKK
HHHHHHHHHCC
>Mature Secondary Structure
MKLNPRQDEAVKYVSGPCLVLAGAGSGKTRVITNKIAYLVQQCGYKARNIAAVTFTNKAA
CCCCCCCHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHCEEEEEECCHHH
REMKERVAQTLGKGESRGLMVSTFHTLGLNIIRREFKALGLKAGFSLFDDQDQLALLKEL
HHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
TEKQLDGDKDLLRLLLSTISNWKNDMLTPPQAKAMAKGEQQLLFAHCFELYQKQMQSYNA
HHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
LDFDDLILLPVLLLRSNEEVRQRWQNRIRYLLVDEYQDTNTSQYELVKLLVGERGRLTVV
CCHHHHHHHHHHHHCCCHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEE
GDDDQSIYSWRGAKPQNLVLLGEDFPSLKLIKLEQNYRSTSRILRAANILIANNPHVYQK
ECCCCHHHHCCCCCCCCEEEECCCCCCEEEEEECHHHHHHHHHHHHHHEEEECCCHHHHH
ALFSELAEGEKLKVILANNEDHEAERVIAEIIAHKFLNRTEYRDYAILYRGNHQSRLIEK
HHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHH
SLTQNRVPYKLSGGTSFFARAEIKDIMAYLRVLVNPDDDNAFLRIVNTPKREIGPATLEK
HHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHCCHHHHHH
LGSYANMRGKSLFTASFELGLEQHLSGRGLENLRRFTEWLVAIADNAERGNTVEAVRALV
HHHHHCCCCCEEEEHHHHHCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
RDIRYEDWLYETSASPKAAEMRMKNVSDLYSWIVADLEGDNPDQQEKTLKEVVQRLTLRD
HHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
MMERGEENDDSDAVQLMTLHASKGLEFPYVYLIGAEEGILPHQTSIDEENVEEERRLMYV
HHHCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHEEEEE
GITRAQRELTFIVCKERRQFGELIKPSQSRFLDELPQDDIEWEVKKKPVTQEERMAKGQA
EEHHHHHHEEEEEEHHHHHHHHHCCCCHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHH
HIANLRAMFKK
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]