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The map label for this gene is rhtB [H]

Identifier: 227080372

GI number: 227080372

Start: 130713

End: 131330

Strand: Direct

Name: rhtB [H]

Synonym: VCM66_0136

Alternate gene names: 227080372

Gene position: 130713-131330 (Clockwise)

Preceding gene: 227080369

Following gene: 227080373

Centisome position: 4.52

GC content: 47.9

Gene sequence:

>618_bases
ATGGATATCCATGTTTGGCTTGCCTATCTACTGACCGCGGTGGTGTTTAGTCTGGCGCCCGGTTCAGGCACGGTTAACTC
GATCAGCAATGGTTTAAGTTATGGTACACGCCACTCATTAGGGGCGATTATCGGCCTACAAATCGGTCTTGCTTGTCATA
TAGTGTTGGTCGGCATTGGAATTGGCGCTTTGGTGGCACAATCTGCGTTGGCGTTCACTCTGATCAAATGGATTGGCGCG
GCTTATTTAGTATGGCTGGGTATTCAGAAATGGCGAGATAGAGCCCCCCTAACGGCTACCACAACTTCTCATGAACTCTC
ACAAGCCGCCCTGCTGCGTAAAGCAGTACTGATTAACCTGACCAATCCCAAATCAATCGTTTTCTTAGTGGCATTATTTC
CGCAGTTTATTGATCCCACTAGAGATCATTGGCCGCAGTTCTTGGTTCTGGGTATCACAACCGTTACTATTGATGCGATT
GTCATGTTCGGATACACGGCACTGGCTGCGCAGCTAGGTCGCTATATTCGTTCACCTAACATAATGACCAGAATGAATAA
ACTGTTTGGTTCGATGTTTATGGGCTGCGGAATGCTGTTGGCTACCGCCAAAGCGTAA

Upstream 100 bases:

>100_bases
GGATAAAGATTTTTTTCAGGGTTCATAAGTTGGCAATGGGGTTGTGCGAATCAGTGTTTGACCTCATTGGGCTAGCATAA
GTCAGGAATGAGGAATTGCA

Downstream 100 bases:

>100_bases
GCAACCCTTTGAGGACACCATGTACACAACTTATGTAGCACGCCAACCGATATTGAATGCAAAGCGACACACGCTAGGTT
ATGAGCTGCTGTTTCGAGAT

Product: homoserine/homoserine lactone efflux protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MDIHVWLAYLLTAVVFSLAPGSGTVNSISNGLSYGTRHSLGAIIGLQIGLACHIVLVGIGIGALVAQSALAFTLIKWIGA
AYLVWLGIQKWRDRAPLTATTTSHELSQAALLRKAVLINLTNPKSIVFLVALFPQFIDPTRDHWPQFLVLGITTVTIDAI
VMFGYTALAAQLGRYIRSPNIMTRMNKLFGSMFMGCGMLLATAKA

Sequences:

>Translated_205_residues
MDIHVWLAYLLTAVVFSLAPGSGTVNSISNGLSYGTRHSLGAIIGLQIGLACHIVLVGIGIGALVAQSALAFTLIKWIGA
AYLVWLGIQKWRDRAPLTATTTSHELSQAALLRKAVLINLTNPKSIVFLVALFPQFIDPTRDHWPQFLVLGITTVTIDAI
VMFGYTALAAQLGRYIRSPNIMTRMNKLFGSMFMGCGMLLATAKA
>Mature_205_residues
MDIHVWLAYLLTAVVFSLAPGSGTVNSISNGLSYGTRHSLGAIIGLQIGLACHIVLVGIGIGALVAQSALAFTLIKWIGA
AYLVWLGIQKWRDRAPLTATTTSHELSQAALLRKAVLINLTNPKSIVFLVALFPQFIDPTRDHWPQFLVLGITTVTIDAI
VMFGYTALAAQLGRYIRSPNIMTRMNKLFGSMFMGCGMLLATAKA

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=204, Percent_Identity=50, Blast_Score=217, Evalue=4e-58,
Organism=Escherichia coli, GI1788099, Length=210, Percent_Identity=30, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22100; Mature: 22100

Theoretical pI: Translated: 10.39; Mature: 10.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIHVWLAYLLTAVVFSLAPGSGTVNSISNGLSYGTRHSLGAIIGLQIGLACHIVLVGIG
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IGALVAQSALAFTLIKWIGAAYLVWLGIQKWRDRAPLTATTTSHELSQAALLRKAVLINL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHEEC
TNPKSIVFLVALFPQFIDPTRDHWPQFLVLGITTVTIDAIVMFGYTALAAQLGRYIRSPN
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
IMTRMNKLFGSMFMGCGMLLATAKA
HHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDIHVWLAYLLTAVVFSLAPGSGTVNSISNGLSYGTRHSLGAIIGLQIGLACHIVLVGIG
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IGALVAQSALAFTLIKWIGAAYLVWLGIQKWRDRAPLTATTTSHELSQAALLRKAVLINL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHEEC
TNPKSIVFLVALFPQFIDPTRDHWPQFLVLGITTVTIDAIVMFGYTALAAQLGRYIRSPN
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
IMTRMNKLFGSMFMGCGMLLATAKA
HHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]