Click here to switch to the map view.

The map label for this gene is yegE [H]

Identifier: 227080308

GI number: 227080308

Start: 71477

End: 73783

Strand: Direct

Name: yegE [H]

Synonym: VCM66_0072

Alternate gene names: 227080308

Gene position: 71477-73783 (Clockwise)

Preceding gene: 227080306

Following gene: 227080309

Centisome position: 2.47

GC content: 50.07

Gene sequence:

>2307_bases
TTGGAGTCTGACTTGAGCCGATTGCGACACTGGTACAACAAATATGCGCTGCCTCTTGCTGTTGGGTTGGGCTGCTTTTG
GACGGCCAACCTAGTCGCGCAAGAACCAACCTTATCGAGCCAACCGTTAGACAATCTAGGAACGAGTCGCGTGCCATGGG
ACAGCGAGCTTGCAGCAAACGCCATGTTGTGGATTGTGGCGCTTGTCGCCATCATGGTTTTAAGCATCTTAACAGTGCGC
CAGTACCACTATATTCAACGCTTACGCACCAACCAAAAATTACTGGAGTCGATCTTTGATCAAAGCACACACTACATCGG
TATCTTTGACTTAGAAGGTCGGATTATTTCCTGCAATGGCAAGTTACAGGGACTGCTGTATCGACATGGTGAAAGTTTGC
TAAGACCGATCTGGCAGCACAAAGGTTGGGAGGATTCAGCGGTCGAACACATTCAGAACTATTTCTCTGAGTCAGTGCCG
CAAACTCGCCAGTTTAACGCCGAAATTTGGCATCCTGAGCTTGGAGCCATCGTGCTGGAGTGTCAGTTTAAGCCGCTACC
GACTCGCGAAGAGTCGCAAATTCTGCTTGAAGCACAAGACATTACTTGGCGAAAAATTACCGAAGATAAACTCTTTCAGC
GTGAAGCCAGCCTTCGCCATTATTACGATCAGCAGCCAGTGATGATGCTAACCTTGGATGAGCGTAACCACATTCAACAA
GTGAATCATTTTGCTGAACAGCTGCTGGGTTATCCCGCCGATGTCATGCTTGGGCACCATATCCGCGATTTTTATCAAGA
TGAAGAGGTACTGACACCAAGGCAGATGATACTGCTACCGAGCCGCCATGCTTCTTCTGTTTGGCGGCGAGAAGTGTGTT
ATCGCCATCACTCCGGTGAAACGGTGTGGGTACGGGAGAACATTCGTCCGCTGGTCGAAACGGGCACGCTGTTAATCGTT
GGGGAAGACATTACCGAAACCCGCCAGCTTGCCGACCAACTGGCGTATCAAGCTCGCTATGATTTATTGACCCACACTCT
AAACCGAAACCAGTTTGAGCTAGAACTGGCCAAGGCGCTGAAAGAAACCGACAGTCAATTACGCACCCACGCCATGCTGT
ATCTCGATCTCGATCAGCTTAAAGTGCTCAACGATACCGCGGGCCATGATGCAGGCGATGGCGCGATTCAGTTCTGTGCG
AGCATGCTGGAAGATGTGTTGCCCTTTAAAGCCACCTTGGCGCGTATGGGCGGCGATGAGTTTTCCGTATTACTGCGTGA
TTGCACCGAGCGTGATGCAGTGTTGGTGGCGCAAAGTATTATCCATGCGCTCAGTGAAGTGGCGTTTGTCTGGGAGCATA
TTCGCTTTAATCTGACCTGTTCGATCGGGATCCGTATGATTGACCATACTGCGACATCACCACAAATGGTGCATGCACAA
GCGGATACGGCGTGCCATGCCGCCAAAGAGGAAGGACGCAATCGCTTCAACCTCTATCGACAGGATGATGAGGATTTGCG
CCGGCGCCAATTGGAAATGGAGTCGGTGAATCTAGTGCACGATGCGCTCGCCAATGAGCGAATTGAGCTATTTGCTCAGC
GCATTGTGCCGCTCAATCAACCCGATGTGTTGCTTCATTTCGAAGTCTTAGTGCGAATTAAAAACGCCGCAGGGGAGTAC
GTTTCACCGGGCATTTTCGTTCCCGCCTCTGAGCGTTATAATTTGGCGCACCGTCTGGATCGGAAAATTGTCGAGCAGAC
CTTTAACTGGTTGGAAGCGCGTCCGCAAGTGTTAGATAAACTGGGGCGCGTTTCGATTAACCTATCGGGTAACTCGATTG
GCAATCCAGATTTTGTGGCCTTTCTGCTCGAAAGATTGCGCGATAGCCGCATTCCATGCAGTAAAGTCTGTTTTGAAATC
ACCGAAACGGCTGCGATGCGCAATCTCAATCAAGCGATCAAAGTGTTATCTCAGCTCAAAAGCTTAGGTTGCGTGTTGGC
TCTGGATGATTTTGGCTCGGGGCTCTCCTCGTTCGGTTATCTGCAGAAACTGCCGGTCGATATCGTCAAAATTGATGGCA
TTTTTGTGTGTGATATGGACAAAAATGAGATGGATCGCTTGATGGTCCGCTCTATCCATGAATTGACCAAGCAGATGGGC
AAAAGCACCGTGGCGGAGTTTGTTGAAAACCAGCAGATACTTGAGGCTTTGCAGCAGATTGGTGTGGATTACGCGCAAGG
CTATCTATTCAGCCGCCCACAACCGATCGCTGATTGGGTTGCCGAGCAACTCAAAGAGAAAGCGTAG

Upstream 100 bases:

>100_bases
CATCGAGTTTGGTGGTGGTTTGCATTGTCTTTCCTGACAGTCATGCCGACATGAAATTAAAGTGTGCTTACTTTACCACC
ATTAAAACGATTGTTTAACC

Downstream 100 bases:

>100_bases
GGTTTGCCGATAAAATCCGCTACACTGAGCGCCGTTTTATCTCGGTTTTCGGAGTTCCCTTGTTGATTCAACTGATTTGC
CAAGCGCCTCAACGTGCGCA

Product: GGDEF/EAL family protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 768; Mature: 768

Protein sequence:

>768_residues
MESDLSRLRHWYNKYALPLAVGLGCFWTANLVAQEPTLSSQPLDNLGTSRVPWDSELAANAMLWIVALVAIMVLSILTVR
QYHYIQRLRTNQKLLESIFDQSTHYIGIFDLEGRIISCNGKLQGLLYRHGESLLRPIWQHKGWEDSAVEHIQNYFSESVP
QTRQFNAEIWHPELGAIVLECQFKPLPTREESQILLEAQDITWRKITEDKLFQREASLRHYYDQQPVMMLTLDERNHIQQ
VNHFAEQLLGYPADVMLGHHIRDFYQDEEVLTPRQMILLPSRHASSVWRREVCYRHHSGETVWVRENIRPLVETGTLLIV
GEDITETRQLADQLAYQARYDLLTHTLNRNQFELELAKALKETDSQLRTHAMLYLDLDQLKVLNDTAGHDAGDGAIQFCA
SMLEDVLPFKATLARMGGDEFSVLLRDCTERDAVLVAQSIIHALSEVAFVWEHIRFNLTCSIGIRMIDHTATSPQMVHAQ
ADTACHAAKEEGRNRFNLYRQDDEDLRRRQLEMESVNLVHDALANERIELFAQRIVPLNQPDVLLHFEVLVRIKNAAGEY
VSPGIFVPASERYNLAHRLDRKIVEQTFNWLEARPQVLDKLGRVSINLSGNSIGNPDFVAFLLERLRDSRIPCSKVCFEI
TETAAMRNLNQAIKVLSQLKSLGCVLALDDFGSGLSSFGYLQKLPVDIVKIDGIFVCDMDKNEMDRLMVRSIHELTKQMG
KSTVAEFVENQQILEALQQIGVDYAQGYLFSRPQPIADWVAEQLKEKA

Sequences:

>Translated_768_residues
MESDLSRLRHWYNKYALPLAVGLGCFWTANLVAQEPTLSSQPLDNLGTSRVPWDSELAANAMLWIVALVAIMVLSILTVR
QYHYIQRLRTNQKLLESIFDQSTHYIGIFDLEGRIISCNGKLQGLLYRHGESLLRPIWQHKGWEDSAVEHIQNYFSESVP
QTRQFNAEIWHPELGAIVLECQFKPLPTREESQILLEAQDITWRKITEDKLFQREASLRHYYDQQPVMMLTLDERNHIQQ
VNHFAEQLLGYPADVMLGHHIRDFYQDEEVLTPRQMILLPSRHASSVWRREVCYRHHSGETVWVRENIRPLVETGTLLIV
GEDITETRQLADQLAYQARYDLLTHTLNRNQFELELAKALKETDSQLRTHAMLYLDLDQLKVLNDTAGHDAGDGAIQFCA
SMLEDVLPFKATLARMGGDEFSVLLRDCTERDAVLVAQSIIHALSEVAFVWEHIRFNLTCSIGIRMIDHTATSPQMVHAQ
ADTACHAAKEEGRNRFNLYRQDDEDLRRRQLEMESVNLVHDALANERIELFAQRIVPLNQPDVLLHFEVLVRIKNAAGEY
VSPGIFVPASERYNLAHRLDRKIVEQTFNWLEARPQVLDKLGRVSINLSGNSIGNPDFVAFLLERLRDSRIPCSKVCFEI
TETAAMRNLNQAIKVLSQLKSLGCVLALDDFGSGLSSFGYLQKLPVDIVKIDGIFVCDMDKNEMDRLMVRSIHELTKQMG
KSTVAEFVENQQILEALQQIGVDYAQGYLFSRPQPIADWVAEQLKEKA
>Mature_768_residues
MESDLSRLRHWYNKYALPLAVGLGCFWTANLVAQEPTLSSQPLDNLGTSRVPWDSELAANAMLWIVALVAIMVLSILTVR
QYHYIQRLRTNQKLLESIFDQSTHYIGIFDLEGRIISCNGKLQGLLYRHGESLLRPIWQHKGWEDSAVEHIQNYFSESVP
QTRQFNAEIWHPELGAIVLECQFKPLPTREESQILLEAQDITWRKITEDKLFQREASLRHYYDQQPVMMLTLDERNHIQQ
VNHFAEQLLGYPADVMLGHHIRDFYQDEEVLTPRQMILLPSRHASSVWRREVCYRHHSGETVWVRENIRPLVETGTLLIV
GEDITETRQLADQLAYQARYDLLTHTLNRNQFELELAKALKETDSQLRTHAMLYLDLDQLKVLNDTAGHDAGDGAIQFCA
SMLEDVLPFKATLARMGGDEFSVLLRDCTERDAVLVAQSIIHALSEVAFVWEHIRFNLTCSIGIRMIDHTATSPQMVHAQ
ADTACHAAKEEGRNRFNLYRQDDEDLRRRQLEMESVNLVHDALANERIELFAQRIVPLNQPDVLLHFEVLVRIKNAAGEY
VSPGIFVPASERYNLAHRLDRKIVEQTFNWLEARPQVLDKLGRVSINLSGNSIGNPDFVAFLLERLRDSRIPCSKVCFEI
TETAAMRNLNQAIKVLSQLKSLGCVLALDDFGSGLSSFGYLQKLPVDIVKIDGIFVCDMDKNEMDRLMVRSIHELTKQMG
KSTVAEFVENQQILEALQQIGVDYAQGYLFSRPQPIADWVAEQLKEKA

Specific function: Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria [H]

COG id: COG2202

COG function: function code T; FOG: PAS/PAC domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788381, Length=568, Percent_Identity=27.8169014084507, Blast_Score=209, Evalue=7e-55,
Organism=Escherichia coli, GI1787541, Length=545, Percent_Identity=26.9724770642202, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1788849, Length=276, Percent_Identity=33.695652173913, Blast_Score=150, Evalue=3e-37,
Organism=Escherichia coli, GI87082096, Length=244, Percent_Identity=36.4754098360656, Blast_Score=150, Evalue=3e-37,
Organism=Escherichia coli, GI226510982, Length=419, Percent_Identity=27.9236276849642, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1786507, Length=271, Percent_Identity=30.2583025830258, Blast_Score=107, Evalue=4e-24,
Organism=Escherichia coli, GI87081845, Length=233, Percent_Identity=32.618025751073, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI87081743, Length=251, Percent_Identity=29.8804780876494, Blast_Score=102, Evalue=1e-22,
Organism=Escherichia coli, GI87081921, Length=448, Percent_Identity=23.4375, Blast_Score=100, Evalue=5e-22,
Organism=Escherichia coli, GI1790496, Length=269, Percent_Identity=27.1375464684015, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI87081980, Length=247, Percent_Identity=29.1497975708502, Blast_Score=93, Evalue=6e-20,
Organism=Escherichia coli, GI1787802, Length=192, Percent_Identity=30.2083333333333, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1787055, Length=233, Percent_Identity=27.4678111587983, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788502, Length=214, Percent_Identity=27.1028037383178, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1787262, Length=226, Percent_Identity=27.8761061946903, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1789650, Length=428, Percent_Identity=23.1308411214953, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1786584, Length=169, Percent_Identity=30.7692307692308, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI87081881, Length=277, Percent_Identity=25.6317689530686, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI87082007, Length=169, Percent_Identity=29.585798816568, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI145693134, Length=189, Percent_Identity=30.1587301587302, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR007895
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013656
- InterPro:   IPR013655 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF05231 MASE1; PF08447 PAS_3; PF08448 PAS_4 [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 88090; Mature: 88090

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS00013 PROKAR_LIPOPROTEIN ; PS50112 PAS ; PS50883 EAL ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESDLSRLRHWYNKYALPLAVGLGCFWTANLVAQEPTLSSQPLDNLGTSRVPWDSELAAN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCHHHHHH
AMLWIVALVAIMVLSILTVRQYHYIQRLRTNQKLLESIFDQSTHYIGIFDLEGRIISCNG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEECC
KLQGLLYRHGESLLRPIWQHKGWEDSAVEHIQNYFSESVPQTRQFNAEIWHPELGAIVLE
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCEEEEE
CQFKPLPTREESQILLEAQDITWRKITEDKLFQREASLRHYYDQQPVMMLTLDERNHIQQ
EECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHH
VNHFAEQLLGYPADVMLGHHIRDFYQDEEVLTPRQMILLPSRHASSVWRREVCYRHHSGE
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHEEECCHHHHHHHHHHHHHHHCCCC
TVWVRENIRPLVETGTLLIVGEDITETRQLADQLAYQARYDLLTHTLNRNQFELELAKAL
EEEECCCCCHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KETDSQLRTHAMLYLDLDQLKVLNDTAGHDAGDGAIQFCASMLEDVLPFKATLARMGGDE
HHHHHHHHHHHEEEEEHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHH
FSVLLRDCTERDAVLVAQSIIHALSEVAFVWEHIRFNLTCSIGIRMIDHTATSPQMVHAQ
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEEECCCCCCCEEECH
ADTACHAAKEEGRNRFNLYRQDDEDLRRRQLEMESVNLVHDALANERIELFAQRIVPLNQ
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PDVLLHFEVLVRIKNAAGEYVSPGIFVPASERYNLAHRLDRKIVEQTFNWLEARPQVLDK
CCCEEHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHH
LGRVSINLSGNSIGNPDFVAFLLERLRDSRIPCSKVCFEITETAAMRNLNQAIKVLSQLK
HCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLGCVLALDDFGSGLSSFGYLQKLPVDIVKIDGIFVCDMDKNEMDRLMVRSIHELTKQMG
HCCEEEEECHHCCCHHHCCHHHHCCCEEEEECCEEEEECCHHHHHHHHHHHHHHHHHHHH
KSTVAEFVENQQILEALQQIGVDYAQGYLFSRPQPIADWVAEQLKEKA
HHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MESDLSRLRHWYNKYALPLAVGLGCFWTANLVAQEPTLSSQPLDNLGTSRVPWDSELAAN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCHHHHHH
AMLWIVALVAIMVLSILTVRQYHYIQRLRTNQKLLESIFDQSTHYIGIFDLEGRIISCNG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEECC
KLQGLLYRHGESLLRPIWQHKGWEDSAVEHIQNYFSESVPQTRQFNAEIWHPELGAIVLE
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCEEEEE
CQFKPLPTREESQILLEAQDITWRKITEDKLFQREASLRHYYDQQPVMMLTLDERNHIQQ
EECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHH
VNHFAEQLLGYPADVMLGHHIRDFYQDEEVLTPRQMILLPSRHASSVWRREVCYRHHSGE
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHEEECCHHHHHHHHHHHHHHHCCCC
TVWVRENIRPLVETGTLLIVGEDITETRQLADQLAYQARYDLLTHTLNRNQFELELAKAL
EEEECCCCCHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KETDSQLRTHAMLYLDLDQLKVLNDTAGHDAGDGAIQFCASMLEDVLPFKATLARMGGDE
HHHHHHHHHHHEEEEEHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHH
FSVLLRDCTERDAVLVAQSIIHALSEVAFVWEHIRFNLTCSIGIRMIDHTATSPQMVHAQ
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEEECCCCCCCEEECH
ADTACHAAKEEGRNRFNLYRQDDEDLRRRQLEMESVNLVHDALANERIELFAQRIVPLNQ
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PDVLLHFEVLVRIKNAAGEYVSPGIFVPASERYNLAHRLDRKIVEQTFNWLEARPQVLDK
CCCEEHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHH
LGRVSINLSGNSIGNPDFVAFLLERLRDSRIPCSKVCFEITETAAMRNLNQAIKVLSQLK
HCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLGCVLALDDFGSGLSSFGYLQKLPVDIVKIDGIFVCDMDKNEMDRLMVRSIHELTKQMG
HCCEEEEECHHCCCHHHCCHHHHCCCEEEEECCEEEEECCHHHHHHHHHHHHHHHHHHHH
KSTVAEFVENQQILEALQQIGVDYAQGYLFSRPQPIADWVAEQLKEKA
HHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 6094528; 7984428 [H]