Click here to switch to the map view.

The map label for this gene is mdtL [H]

Identifier: 227080305

GI number: 227080305

Start: 69261

End: 70463

Strand: Direct

Name: mdtL [H]

Synonym: VCM66_0069

Alternate gene names: 227080305

Gene position: 69261-70463 (Clockwise)

Preceding gene: 227080302

Following gene: 227080306

Centisome position: 2.39

GC content: 50.12

Gene sequence:

>1203_bases
GTGAAACAAGCAATGCCATCTCAGGTCAGTAAGCAGCAAATGGTGCTACTGACACTACTCGTTCTATTTAGCCCATTGGC
GATCGATATTTATCTACCGGCATTGCCTCAAATATCACAAGCGTTTCACGTGGAACACGCTTTAGCACAAGATACGATTA
CCTGGTTTTTGTTTGCGATGGGCGTCGGGCAGTTATTTGCTGGCCCATTAGCAGATAAGTATGGTCGACGAACTGTTGCA
TTAGGGGGCATTAGCATTTATGCCTTGAGCGCTTTATTGGCGTGGAGTGCTCAAAGTATTGAATGGATGTTAATGGCTCG
GTTGCTACAAGGCTTAGGAGCGTGTGCGACTTCCGTAGCAGCTTTTGCAACAGTTCGCGATTTATTTGGGCCGCAACGCA
GCGGTAAGATGATCAGTTACCTCAATGGAGCGATCTGTTTTATTCCGGCACTCGCGCCGATTCTGGGCAGTTGGTTAACT
CAGCAATTCGGTTGGCGAGCAAACTTCAGTTTTATGACCGGATTTGCGCTGCTGGTAGGGTTAGCCATGGCTCTACGTCT
GCAGGAGAGTAATCCGTACATGGGCAGTCGACCTGCCGTCTTTAAGCTCTCTCGTTATGCCGCCGTATTAAAAGCGCCGA
CCTTCCTCTTCCACGCATCGCTGTGTCTGCTGGCGATGGCGGTGATTCTCGCTTACGTGACCTCCGCGCCAGTGATTCTC
ATGGAGCGTTTACAGCTCAGCATGAATGAATTCACCTTCTGGTTTGGCGTTAATGCGGTGATCAATATCATTGCCTGCAT
GACGGCCCCCAAAGTGATGGATAAATTGGGTACTCGTTGGTCTTTGATCATTGGCATTACTACGTTAATGATCGCTGGCA
GTCTAATGCTGGTGCTGCGTAATCAGGCAAGCGCACTGGCGTTCATGTTGCCAGTGTTTTTATCATCGATTGGTTTTGCA
TGGATACTGGGCGCAGCCGCCGGTAAAGCGTTAGCTCCGTTTGGCGATAAAGCCGGTACGGCGGCGGCATTGCTCGGACT
ATTCCAGATGAGTGGTTCTGGGCTATTGGTCGGTACGTTGCAACGCATTATGCCTGAGCCGCAACTACTGATCGGTTTGA
CCATGTGGCTAGCGGCACCTGCGTTACTCATTTTATTCTCACCGCTTGGTTTTCGTTGGCACGCCAGTGTCTCTGAGTGT
TGA

Upstream 100 bases:

>100_bases
TTTTCCAAAATAGAATAACAGCAATGACGATATACCACTGTTTAGCATATCTTGAGCCACGTATCATGCACCGATCTTTA
AAATTCAGTCGGAGTGTGAA

Downstream 100 bases:

>100_bases
TAGAACAAGATAAATTCACGCTGAGGCTATTTTACTCAGCGTGCATCCTGTGTATATTGTTTGCCTCTTCGATACTGACT
AACCGTTTGGAATCCAAAAC

Product: putative multidrug resistance protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MKQAMPSQVSKQQMVLLTLLVLFSPLAIDIYLPALPQISQAFHVEHALAQDTITWFLFAMGVGQLFAGPLADKYGRRTVA
LGGISIYALSALLAWSAQSIEWMLMARLLQGLGACATSVAAFATVRDLFGPQRSGKMISYLNGAICFIPALAPILGSWLT
QQFGWRANFSFMTGFALLVGLAMALRLQESNPYMGSRPAVFKLSRYAAVLKAPTFLFHASLCLLAMAVILAYVTSAPVIL
MERLQLSMNEFTFWFGVNAVINIIACMTAPKVMDKLGTRWSLIIGITTLMIAGSLMLVLRNQASALAFMLPVFLSSIGFA
WILGAAAGKALAPFGDKAGTAAALLGLFQMSGSGLLVGTLQRIMPEPQLLIGLTMWLAAPALLILFSPLGFRWHASVSEC

Sequences:

>Translated_400_residues
MKQAMPSQVSKQQMVLLTLLVLFSPLAIDIYLPALPQISQAFHVEHALAQDTITWFLFAMGVGQLFAGPLADKYGRRTVA
LGGISIYALSALLAWSAQSIEWMLMARLLQGLGACATSVAAFATVRDLFGPQRSGKMISYLNGAICFIPALAPILGSWLT
QQFGWRANFSFMTGFALLVGLAMALRLQESNPYMGSRPAVFKLSRYAAVLKAPTFLFHASLCLLAMAVILAYVTSAPVIL
MERLQLSMNEFTFWFGVNAVINIIACMTAPKVMDKLGTRWSLIIGITTLMIAGSLMLVLRNQASALAFMLPVFLSSIGFA
WILGAAAGKALAPFGDKAGTAAALLGLFQMSGSGLLVGTLQRIMPEPQLLIGLTMWLAAPALLILFSPLGFRWHASVSEC
>Mature_400_residues
MKQAMPSQVSKQQMVLLTLLVLFSPLAIDIYLPALPQISQAFHVEHALAQDTITWFLFAMGVGQLFAGPLADKYGRRTVA
LGGISIYALSALLAWSAQSIEWMLMARLLQGLGACATSVAAFATVRDLFGPQRSGKMISYLNGAICFIPALAPILGSWLT
QQFGWRANFSFMTGFALLVGLAMALRLQESNPYMGSRPAVFKLSRYAAVLKAPTFLFHASLCLLAMAVILAYVTSAPVIL
MERLQLSMNEFTFWFGVNAVINIIACMTAPKVMDKLGTRWSLIIGITTLMIAGSLMLVLRNQASALAFMLPVFLSSIGFA
WILGAAAGKALAPFGDKAGTAAALLGLFQMSGSGLLVGTLQRIMPEPQLLIGLTMWLAAPALLILFSPLGFRWHASVSEC

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. MdtL (TC 2.A.1.2.22) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790146, Length=336, Percent_Identity=31.25, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1788509, Length=354, Percent_Identity=27.1186440677966, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI87082312, Length=345, Percent_Identity=28.1159420289855, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI48994889, Length=351, Percent_Identity=27.6353276353276, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790794, Length=230, Percent_Identity=26.0869565217391, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787065, Length=254, Percent_Identity=23.2283464566929, Blast_Score=72, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6324264, Length=158, Percent_Identity=28.4810126582279, Blast_Score=74, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43094; Mature: 43094

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQAMPSQVSKQQMVLLTLLVLFSPLAIDIYLPALPQISQAFHVEHALAQDTITWFLFAM
CCCCCCHHHHHHHHHHHHHHHHHCCHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GVGQLFAGPLADKYGRRTVALGGISIYALSALLAWSAQSIEWMLMARLLQGLGACATSVA
HHHHHHHCHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFATVRDLFGPQRSGKMISYLNGAICFIPALAPILGSWLTQQFGWRANFSFMTGFALLVG
HHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LAMALRLQESNPYMGSRPAVFKLSRYAAVLKAPTFLFHASLCLLAMAVILAYVTSAPVIL
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
MERLQLSMNEFTFWFGVNAVINIIACMTAPKVMDKLGTRWSLIIGITTLMIAGSLMLVLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
NQASALAFMLPVFLSSIGFAWILGAAAGKALAPFGDKAGTAAALLGLFQMSGSGLLVGTL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHH
QRIMPEPQLLIGLTMWLAAPALLILFSPLGFRWHASVSEC
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCC
>Mature Secondary Structure
MKQAMPSQVSKQQMVLLTLLVLFSPLAIDIYLPALPQISQAFHVEHALAQDTITWFLFAM
CCCCCCHHHHHHHHHHHHHHHHHCCHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GVGQLFAGPLADKYGRRTVALGGISIYALSALLAWSAQSIEWMLMARLLQGLGACATSVA
HHHHHHHCHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFATVRDLFGPQRSGKMISYLNGAICFIPALAPILGSWLTQQFGWRANFSFMTGFALLVG
HHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LAMALRLQESNPYMGSRPAVFKLSRYAAVLKAPTFLFHASLCLLAMAVILAYVTSAPVIL
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
MERLQLSMNEFTFWFGVNAVINIIACMTAPKVMDKLGTRWSLIIGITTLMIAGSLMLVLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
NQASALAFMLPVFLSSIGFAWILGAAAGKALAPFGDKAGTAAALLGLFQMSGSGLLVGTL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHH
QRIMPEPQLLIGLTMWLAAPALLILFSPLGFRWHASVSEC
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA