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The map label for this gene is thiH [H]

Identifier: 227080302

GI number: 227080302

Start: 64952

End: 66064

Strand: Direct

Name: thiH [H]

Synonym: VCM66_0066

Alternate gene names: 227080302

Gene position: 64952-66064 (Clockwise)

Preceding gene: 227080301

Following gene: 227080305

Centisome position: 2.25

GC content: 47.8

Gene sequence:

>1113_bases
ATGAGCTTCATTACCCACTTCCAACAATTAGGCTGGGATGACAGTCGGCTATCGATTTATGGTAAGACAGCGCGGGATGT
TGAACGCGCTTTATCTTCACCTAAACGGACGTTAGACGATTTCAAAGCGCTGATTTCACCTTCAGCAGAGCCTTATCTGG
AAACAATGGCGCAAATGGCTTATCAGGCCACTCGTCAACGTTTTGGCAATACCATGTCGATGTACATTCCGCTCTACCTC
TCTAATCTCTGTTCGAATTCCTGTACCTATTGCGGTTTTTCAATGGATAACCGAATCAAGCGTAAAACACTGAATGAAGT
GGAGATTGAACGAGAGATCGCGGCGATTAAACGAATGGGTTTTGATAGCGTTTTGCTGGTGACCGGAGAGCATGAAACCA
AAGTGGGTATCGAATACTTCCGTCGGGCACTGCCTATCATCAAGCAAGCTTTTCATTATGTTGCGATGGAAGTTCAGCCG
TTGCAGCAAGAAGAGTATGCCGAGTTAATTGGGCTCGGGTTGGATGCGGTGATGGTTTACCAAGAAACGTACCATCCTTC
GACTTATGCGCAGCATCATTTGCGAGGCAAGAAAACCGATTTTTGGTATCGACTCGAAACACCCGATAGGTTGGCTAGAG
CGGGCATCGATAAAATTGGTATTGGCGCGCTGATTGGGCTTGAGGACTGGCGAACTGACAGCATTTTTGTTGCGGCGCAT
CTGGATTATCTTGAGCGACAATATTGGAAGACGCGCTACTCAATTTCTTTTCCGCGTTTACGCCCGTGTGAGGGCGCTTT
ACAGCCGAAATCAGTGATGACGGATCGACAACTTGTGCAGTTGATTTGTGCATTTCGTCTGTTCAATAGCGAGGTTGAGC
TTTCTTTGTCTACCCGCGAGTCACCGATGTTTCGTGATCAAGTGGCTAAGCTCGGGATTACCTCAATGTCTGCAGCGTCT
AAAACTCAGCCGGGAGGGTATTCAGACCCTAAGGTTGAGTTAGAGCAGTTTGCGGTAAGTGATGAGCGTTCTGCCGCAGA
GGTGAGTAGCGCGTTAATGGAGCAAGGTTTACAAGTGGTATGGCATGACTGGCATCGAGCCTATTCAGGCTAA

Upstream 100 bases:

>100_bases
CGGTCGAAGCTGGGCGAATGGCTTATGAATCCGGCTTGCCATCACGAGTAAAAATGGCGACGGCATCAAGTCCATTAACT
GGATTTTTGGATTTTGTATC

Downstream 100 bases:

>100_bases
TGTTTCACTCAAATTAATGTTTCACGTGAAACCCAAAGCTTGTATTTTACAAAAACGGCATTTGAGATTCGGATAGAGCC
TTTTCTGGGTTGAAACTTGG

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MSFITHFQQLGWDDSRLSIYGKTARDVERALSSPKRTLDDFKALISPSAEPYLETMAQMAYQATRQRFGNTMSMYIPLYL
SNLCSNSCTYCGFSMDNRIKRKTLNEVEIEREIAAIKRMGFDSVLLVTGEHETKVGIEYFRRALPIIKQAFHYVAMEVQP
LQQEEYAELIGLGLDAVMVYQETYHPSTYAQHHLRGKKTDFWYRLETPDRLARAGIDKIGIGALIGLEDWRTDSIFVAAH
LDYLERQYWKTRYSISFPRLRPCEGALQPKSVMTDRQLVQLICAFRLFNSEVELSLSTRESPMFRDQVAKLGITSMSAAS
KTQPGGYSDPKVELEQFAVSDERSAAEVSSALMEQGLQVVWHDWHRAYSG

Sequences:

>Translated_370_residues
MSFITHFQQLGWDDSRLSIYGKTARDVERALSSPKRTLDDFKALISPSAEPYLETMAQMAYQATRQRFGNTMSMYIPLYL
SNLCSNSCTYCGFSMDNRIKRKTLNEVEIEREIAAIKRMGFDSVLLVTGEHETKVGIEYFRRALPIIKQAFHYVAMEVQP
LQQEEYAELIGLGLDAVMVYQETYHPSTYAQHHLRGKKTDFWYRLETPDRLARAGIDKIGIGALIGLEDWRTDSIFVAAH
LDYLERQYWKTRYSISFPRLRPCEGALQPKSVMTDRQLVQLICAFRLFNSEVELSLSTRESPMFRDQVAKLGITSMSAAS
KTQPGGYSDPKVELEQFAVSDERSAAEVSSALMEQGLQVVWHDWHRAYSG
>Mature_369_residues
SFITHFQQLGWDDSRLSIYGKTARDVERALSSPKRTLDDFKALISPSAEPYLETMAQMAYQATRQRFGNTMSMYIPLYLS
NLCSNSCTYCGFSMDNRIKRKTLNEVEIEREIAAIKRMGFDSVLLVTGEHETKVGIEYFRRALPIIKQAFHYVAMEVQPL
QQEEYAELIGLGLDAVMVYQETYHPSTYAQHHLRGKKTDFWYRLETPDRLARAGIDKIGIGALIGLEDWRTDSIFVAAHL
DYLERQYWKTRYSISFPRLRPCEGALQPKSVMTDRQLVQLICAFRLFNSEVELSLSTRESPMFRDQVAKLGITSMSAASK
TQPGGYSDPKVELEQFAVSDERSAAEVSSALMEQGLQVVWHDWHRAYSG

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=364, Percent_Identity=64.8351648351648, Blast_Score=498, Evalue=1e-142,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 42386; Mature: 42255

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFITHFQQLGWDDSRLSIYGKTARDVERALSSPKRTLDDFKALISPSAEPYLETMAQMA
CCHHHHHHHCCCCCCEEEEECCHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHH
YQATRQRFGNTMSMYIPLYLSNLCSNSCTYCGFSMDNRIKRKTLNEVEIEREIAAIKRMG
HHHHHHHHCCHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCC
FDSVLLVTGEHETKVGIEYFRRALPIIKQAFHYVAMEVQPLQQEEYAELIGLGLDAVMVY
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHEEH
QETYHPSTYAQHHLRGKKTDFWYRLETPDRLARAGIDKIGIGALIGLEDWRTDSIFVAAH
HHHCCCHHHHHHHHCCCCCCEEEEECCHHHHHHCCCCCCCCHHEEECCCCCCCCEEEEHH
LDYLERQYWKTRYSISFPRLRPCEGALQPKSVMTDRQLVQLICAFRLFNSEVELSLSTRE
HHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
SPMFRDQVAKLGITSMSAASKTQPGGYSDPKVELEQFAVSDERSAAEVSSALMEQGLQVV
CCHHHHHHHHHCCHHHCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCHHHH
WHDWHRAYSG
HHHHHHHCCC
>Mature Secondary Structure 
SFITHFQQLGWDDSRLSIYGKTARDVERALSSPKRTLDDFKALISPSAEPYLETMAQMA
CHHHHHHHCCCCCCEEEEECCHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHH
YQATRQRFGNTMSMYIPLYLSNLCSNSCTYCGFSMDNRIKRKTLNEVEIEREIAAIKRMG
HHHHHHHHCCHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCC
FDSVLLVTGEHETKVGIEYFRRALPIIKQAFHYVAMEVQPLQQEEYAELIGLGLDAVMVY
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHEEH
QETYHPSTYAQHHLRGKKTDFWYRLETPDRLARAGIDKIGIGALIGLEDWRTDSIFVAAH
HHHCCCHHHHHHHHCCCCCCEEEEECCHHHHHHCCCCCCCCHHEEECCCCCCCCEEEEHH
LDYLERQYWKTRYSISFPRLRPCEGALQPKSVMTDRQLVQLICAFRLFNSEVELSLSTRE
HHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
SPMFRDQVAKLGITSMSAASKTQPGGYSDPKVELEQFAVSDERSAAEVSSALMEQGLQVV
CCHHHHHHHHHCCHHHCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCHHHH
WHDWHRAYSG
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 10082377 [H]