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The map label for this gene is comM [H]

Identifier: 227080269

GI number: 227080269

Start: 30069

End: 31592

Strand: Direct

Name: comM [H]

Synonym: VCM66_0032

Alternate gene names: 227080269

Gene position: 30069-31592 (Clockwise)

Preceding gene: 227080262

Following gene: 227080270

Centisome position: 1.04

GC content: 53.54

Gene sequence:

>1524_bases
ATGGGACTTGCGATCATTCATAGCCGCGCCAGTATTGGCGTGCAGGCACCTCCGGTCACGGTTGAGGTTCACATCAGTAA
CGGGATGCCGGGGTTTACCTTGGTAGGATTGCCAGAAACCACAGTAAAAGAGTCGCGCGATCGGGTGCGCAGCGCGATCA
TCAATTCTCGCTTTGAGTTTCCAGCGAAGCGCATTACGGTCAATTTAGCGCCAGCCGATTTACCCAAAGAGGGGGGACGT
TTTGATTTGCCGATTGCACTTGGCATTTTGGCGGCCTCGGATCAAATTGCGCGAAATAAGCTCGAAAGTTACGAATTTAT
CGGCGAATTGGCGCTATCGGGCGAAATTCGTGGCGTGAAAGGGGTTTTGCCTGCCGCGCTCGCGGCTAACCAAGTGGAGC
GCTGTTTGGTGGTGCCGCACAGTAATGGTGATCAGGCGGCTTTGGTCGGGGTTGAGCGGCATAAGTCGGCGCAAAGCTTA
CTCGAAGTGTGCGCAGACTTGTGTGGTCAGCAAACTCTATCTTTGTTTCAAAGTTCGCCCAGTGTGCAACAAGTAAGTCA
GACCCGCGATCTGCAAGACATCATAGGTCAGCAGCAGGGAAAACGAGCTTTGGAAATCGCTGCGGCTGGCAATCACAACC
TATTGTTTCTCGGCCCTCCGGGGACGGGTAAAACCATGCTCGCTTCACGGCTGTGCGATTTACTGCCAGAAATGAGTGAT
GAAGAGGCAATGGAAACAGCTTCGATTGCCTCTCTTACGCAGCAAGAGATTAACCAACATAACTGGAAGTTACGCCCTTT
TCGTGCACCGCACCATTCAAGCTCAATGGCGGCTTTGGTCGGGGGCGGCACCATTCCGCGCCCCGGTGAAATTTCTCTCG
CCCATAATGGACTACTGTTTTTGGACGAAATGCCGGAGTTTGAGCGTAAAGTGCTTGATTCACTGCGTGAACCGCTGGAA
TCGGGTGAGATCGTGATTTCGCGCGCGCAGGGGAAAACCCGTTTTCCAGCACGCTTTCAATTGGTCGGCGCGTTAAATCC
AAGCCCCACGGGCTACTACGAAGGCAGTCAGGCGCGTGCCAATCCGCAGAGTATTTTGCGCTATTTGAGTCGGCTTTCGG
GCCCTTTGTTGGATCGCTTTGATATGTCGATTGAGATCCCAGCGTTACCCAAAGGTACGCTCGCCAATGGGGGCGATCGT
GGTGAATCGACGGCGGCTGTGCGCCAGCGAGTGTGGGTAGCGCGTGAGCGTATGTTGGCGCGATCGGGCAAAGTGAATGC
TTTGCTGCAAAGTCGAGAAATTGAACAGTATTGCCCTTTGCTTAAAGCGGATGCGGAATTTCTAGAAAGCGCGCTGCATC
GCTTGGGTTTATCAATTCGGGCTTACCATCGCATCATTAAAGTGGCGCGAACCATAGCGGATTTGCAAGGTGAAGCGCAG
ATCGCTCGTCCACATTTGGCCGAGGCGTTGGGCTATCGAGCCATGGATCGCTTACTCAAACAGCTCAGTGCGCAAAACGT
CTAG

Upstream 100 bases:

>100_bases
ATTCCCGTTTTATCTCACCACGCTATATAACCCATTCTGTGCTTTTTATCGCCACTTATTCAGGCGCTGAAGGCTTTTTG
ATCAAATACTAAGGGAAGCA

Downstream 100 bases:

>100_bases
TACTCTGACCTGCAGAGTTCATGTTTCATTACGATGACAAGTTTGTATAATCACACGCTGTTTTTTTACTCATCTTTGAA
CTGAGAACTATGTTGGCTTA

Product: ComM-related protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MGLAIIHSRASIGVQAPPVTVEVHISNGMPGFTLVGLPETTVKESRDRVRSAIINSRFEFPAKRITVNLAPADLPKEGGR
FDLPIALGILAASDQIARNKLESYEFIGELALSGEIRGVKGVLPAALAANQVERCLVVPHSNGDQAALVGVERHKSAQSL
LEVCADLCGQQTLSLFQSSPSVQQVSQTRDLQDIIGQQQGKRALEIAAAGNHNLLFLGPPGTGKTMLASRLCDLLPEMSD
EEAMETASIASLTQQEINQHNWKLRPFRAPHHSSSMAALVGGGTIPRPGEISLAHNGLLFLDEMPEFERKVLDSLREPLE
SGEIVISRAQGKTRFPARFQLVGALNPSPTGYYEGSQARANPQSILRYLSRLSGPLLDRFDMSIEIPALPKGTLANGGDR
GESTAAVRQRVWVARERMLARSGKVNALLQSREIEQYCPLLKADAEFLESALHRLGLSIRAYHRIIKVARTIADLQGEAQ
IARPHLAEALGYRAMDRLLKQLSAQNV

Sequences:

>Translated_507_residues
MGLAIIHSRASIGVQAPPVTVEVHISNGMPGFTLVGLPETTVKESRDRVRSAIINSRFEFPAKRITVNLAPADLPKEGGR
FDLPIALGILAASDQIARNKLESYEFIGELALSGEIRGVKGVLPAALAANQVERCLVVPHSNGDQAALVGVERHKSAQSL
LEVCADLCGQQTLSLFQSSPSVQQVSQTRDLQDIIGQQQGKRALEIAAAGNHNLLFLGPPGTGKTMLASRLCDLLPEMSD
EEAMETASIASLTQQEINQHNWKLRPFRAPHHSSSMAALVGGGTIPRPGEISLAHNGLLFLDEMPEFERKVLDSLREPLE
SGEIVISRAQGKTRFPARFQLVGALNPSPTGYYEGSQARANPQSILRYLSRLSGPLLDRFDMSIEIPALPKGTLANGGDR
GESTAAVRQRVWVARERMLARSGKVNALLQSREIEQYCPLLKADAEFLESALHRLGLSIRAYHRIIKVARTIADLQGEAQ
IARPHLAEALGYRAMDRLLKQLSAQNV
>Mature_506_residues
GLAIIHSRASIGVQAPPVTVEVHISNGMPGFTLVGLPETTVKESRDRVRSAIINSRFEFPAKRITVNLAPADLPKEGGRF
DLPIALGILAASDQIARNKLESYEFIGELALSGEIRGVKGVLPAALAANQVERCLVVPHSNGDQAALVGVERHKSAQSLL
EVCADLCGQQTLSLFQSSPSVQQVSQTRDLQDIIGQQQGKRALEIAAAGNHNLLFLGPPGTGKTMLASRLCDLLPEMSDE
EAMETASIASLTQQEINQHNWKLRPFRAPHHSSSMAALVGGGTIPRPGEISLAHNGLLFLDEMPEFERKVLDSLREPLES
GEIVISRAQGKTRFPARFQLVGALNPSPTGYYEGSQARANPQSILRYLSRLSGPLLDRFDMSIEIPALPKGTLANGGDRG
ESTAAVRQRVWVARERMLARSGKVNALLQSREIEQYCPLLKADAEFLESALHRLGLSIRAYHRIIKVARTIADLQGEAQI
ARPHLAEALGYRAMDRLLKQLSAQNV

Specific function: Induced during competence development. Not needed for DNA uptake. May be involved in a recombination step [H]

COG id: COG0606

COG function: function code O; Predicted ATPase with chaperone activity

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Mg-chelatase subunits D/I family. ComM subfamily [H]

Homologues:

Organism=Escherichia coli, GI145693202, Length=502, Percent_Identity=59.5617529880478, Blast_Score=608, Evalue=1e-175,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001208
- InterPro:   IPR004482
- InterPro:   IPR000523
- InterPro:   IPR020568 [H]

Pfam domain/function: PF01078 Mg_chelatase [H]

EC number: NA

Molecular weight: Translated: 55078; Mature: 54947

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS50051 MCM_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLAIIHSRASIGVQAPPVTVEVHISNGMPGFTLVGLPETTVKESRDRVRSAIINSRFEF
CCEEEEECCCCCCCCCCCEEEEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCC
PAKRITVNLAPADLPKEGGRFDLPIALGILAASDQIARNKLESYEFIGELALSGEIRGVK
CCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCHH
GVLPAALAANQVERCLVVPHSNGDQAALVGVERHKSAQSLLEVCADLCGQQTLSLFQSSP
HHHHHHHHHHHHCEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SVQQVSQTRDLQDIIGQQQGKRALEIAAAGNHNLLFLGPPGTGKTMLASRLCDLLPEMSD
CHHHHHHHHHHHHHHCHHCCCCEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHCCCCC
EEAMETASIASLTQQEINQHNWKLRPFRAPHHSSSMAALVGGGTIPRPGEISLAHNGLLF
HHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCEEEECCCCCCCCCCEEEECCCEEE
LDEMPEFERKVLDSLREPLESGEIVISRAQGKTRFPARFQLVGALNPSPTGYYEGSQARA
ECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCC
NPQSILRYLSRLSGPLLDRFDMSIEIPALPKGTLANGGDRGESTAAVRQRVWVARERMLA
CHHHHHHHHHHHCCCHHHHCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
RSGKVNALLQSREIEQYCPLLKADAEFLESALHRLGLSIRAYHRIIKVARTIADLQGEAQ
HCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHH
IARPHLAEALGYRAMDRLLKQLSAQNV
HCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GLAIIHSRASIGVQAPPVTVEVHISNGMPGFTLVGLPETTVKESRDRVRSAIINSRFEF
CEEEEECCCCCCCCCCCEEEEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCC
PAKRITVNLAPADLPKEGGRFDLPIALGILAASDQIARNKLESYEFIGELALSGEIRGVK
CCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCHH
GVLPAALAANQVERCLVVPHSNGDQAALVGVERHKSAQSLLEVCADLCGQQTLSLFQSSP
HHHHHHHHHHHHCEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SVQQVSQTRDLQDIIGQQQGKRALEIAAAGNHNLLFLGPPGTGKTMLASRLCDLLPEMSD
CHHHHHHHHHHHHHHCHHCCCCEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHCCCCC
EEAMETASIASLTQQEINQHNWKLRPFRAPHHSSSMAALVGGGTIPRPGEISLAHNGLLF
HHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCEEEECCCCCCCCCCEEEECCCEEE
LDEMPEFERKVLDSLREPLESGEIVISRAQGKTRFPARFQLVGALNPSPTGYYEGSQARA
ECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCC
NPQSILRYLSRLSGPLLDRFDMSIEIPALPKGTLANGGDRGESTAAVRQRVWVARERMLA
CHHHHHHHHHHHCCCHHHHCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
RSGKVNALLQSREIEQYCPLLKADAEFLESALHRLGLSIRAYHRIIKVARTIADLQGEAQ
HCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHH
IARPHLAEALGYRAMDRLLKQLSAQNV
HCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800; 9457884 [H]