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The map label for this gene is dnaA [H]

Identifier: 227080249

GI number: 227080249

Start: 7397

End: 8815

Strand: Direct

Name: dnaA [H]

Synonym: VCM66_0012

Alternate gene names: 227080249

Gene position: 7397-8815 (Clockwise)

Preceding gene: 227080248

Following gene: 227080250

Centisome position: 0.26

GC content: 50.67

Gene sequence:

>1419_bases
TTGAGTGAGGGAATCGTGTCATCTTCGCTATGGTTGCAATGTTTGCAACGGCTTCAGGAAGAGCTACCTGCCGCAGAATT
CAGTATGTGGGTGCGTCCGCTTCAAGCGGAGCTCAATGACAATACTCTCACTTTATTCGCCCCGAACCGCTTTGTGTTGG
ATTGGGTACGCGATAAGTACCTCAATAACATCAATCGTCTGCTGATGGAATTCAGTGGCAATGATGTGCCTAATTTGCGC
TTTGAAGTGGGGAGCCGCCCTGTGGTGGCGCCAAAACCCGCGCCTGTACGTACGGCTGCGGATGTCGCGGCGGAATCGTC
GGCGCCTGCGCAATTGGCGCAGCGTAAACCTATCCATAAAACCTGGGATGATGACAGTGCTGCGGCTGATATTACTCACC
GCTCAAATGTGAACCCGAAACACAAGTTCAACAACTTCGTGGAAGGTAAATCTAACCAGTTAGGTCTGGCCGCGGCTCGC
CAAGTCTCTGATAACCCAGGTGCGGCGTATAACCCCCTCTTTTTGTATGGCGGCACCGGTTTGGGTAAAACGCACTTGCT
GCATGCGGTGGGTAACGCGATTGTTGATAACAACCCGAACGCTAAAGTGGTGTACATGCACTCTGAGCGTTTCGTGCAAG
ACATGGTAAAAGCCCTGCAGAACAACGCGATTGAAGAATTCAAACGCTACTATCGCAGTGTAGATGCCTTGTTGATCGAC
GATATTCAATTCTTTGCCAACAAAGAGCGTTCGCAGGAAGAGTTCTTCCACACCTTTAACGCACTGCTGGAAGGCAACCA
ACAAATTATTTTGACTTCTGACCGCTATCCAAAAGAGATCAGTGGTGTAGAAGATCGTCTCAAATCGCGTTTTGGCTGGG
GCTTAACGGTGGCGATCGAGCCGCCGGAGTTGGAAACCCGCGTCGCGATCTTGATGAAAAAAGCGGAAGATCACCAGATT
CATCTGCCGGATGAAGTGGCTTTCTTTATTGCGAAACGCCTGCGCTCTAACGTGCGTGAGTTGGAAGGCGCACTGAACCG
CGTGATTGCCAACGCCAACTTTACCGGTCGCCCAATCACGATTGATTTCGTGCGTGAAGCACTGCGTGACTTATTAGCGC
TGCAAGAAAAGCTGGTCACGATTGATAATATTCAGAAGACCGTTGCCGAATACTACAAAATTAAAGTGGCGGATCTGCTC
TCAAAACGCCGTTCGCGCTCAGTCGCTCGTCCTCGTCAATTGGCGATGGCACTGGCTAAAGAGCTGACCAACCACAGCTT
GCCAGAGATTGGCGATGCGTTTGGCGGCCGTGACCACACGACTGTGTTACACGCTTGCCGTAAAATCGAGCAGTTGCGCG
AAGAGAGTCACGATATCAAAGAAGATTATTCGAACCTGATTCGTACGCTTTCCTCTTAA

Upstream 100 bases:

>100_bases
ATGTGAATAACTTAGATCTTATTCACTGGATCGACGATCCAGCGCTGGCGATCTGAGTTATCAACAGGTAGAATTGTTCC
TCTTTACCGATCGTTGATTT

Downstream 100 bases:

>100_bases
TTTGCGCTATGGTTAGCGCACTTGCGTATTTCGCTTTCACCCGTTTAAGAGAAGACTATGAAATTTACCATTGAACGTAG
CCACTTGATTAAACCATTGC

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSEGIVSSSLWLQCLQRLQEELPAAEFSMWVRPLQAELNDNTLTLFAPNRFVLDWVRDKYLNNINRLLMEFSGNDVPNLR
FEVGSRPVVAPKPAPVRTAADVAAESSAPAQLAQRKPIHKTWDDDSAAADITHRSNVNPKHKFNNFVEGKSNQLGLAAAR
QVSDNPGAAYNPLFLYGGTGLGKTHLLHAVGNAIVDNNPNAKVVYMHSERFVQDMVKALQNNAIEEFKRYYRSVDALLID
DIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEISGVEDRLKSRFGWGLTVAIEPPELETRVAILMKKAEDHQI
HLPDEVAFFIAKRLRSNVRELEGALNRVIANANFTGRPITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVADLL
SKRRSRSVARPRQLAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREESHDIKEDYSNLIRTLSS

Sequences:

>Translated_472_residues
MSEGIVSSSLWLQCLQRLQEELPAAEFSMWVRPLQAELNDNTLTLFAPNRFVLDWVRDKYLNNINRLLMEFSGNDVPNLR
FEVGSRPVVAPKPAPVRTAADVAAESSAPAQLAQRKPIHKTWDDDSAAADITHRSNVNPKHKFNNFVEGKSNQLGLAAAR
QVSDNPGAAYNPLFLYGGTGLGKTHLLHAVGNAIVDNNPNAKVVYMHSERFVQDMVKALQNNAIEEFKRYYRSVDALLID
DIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEISGVEDRLKSRFGWGLTVAIEPPELETRVAILMKKAEDHQI
HLPDEVAFFIAKRLRSNVRELEGALNRVIANANFTGRPITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVADLL
SKRRSRSVARPRQLAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREESHDIKEDYSNLIRTLSS
>Mature_471_residues
SEGIVSSSLWLQCLQRLQEELPAAEFSMWVRPLQAELNDNTLTLFAPNRFVLDWVRDKYLNNINRLLMEFSGNDVPNLRF
EVGSRPVVAPKPAPVRTAADVAAESSAPAQLAQRKPIHKTWDDDSAAADITHRSNVNPKHKFNNFVEGKSNQLGLAAARQ
VSDNPGAAYNPLFLYGGTGLGKTHLLHAVGNAIVDNNPNAKVVYMHSERFVQDMVKALQNNAIEEFKRYYRSVDALLIDD
IQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEISGVEDRLKSRFGWGLTVAIEPPELETRVAILMKKAEDHQIH
LPDEVAFFIAKRLRSNVRELEGALNRVIANANFTGRPITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVADLLS
KRRSRSVARPRQLAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREESHDIKEDYSNLIRTLSS

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family [H]

Homologues:

Organism=Escherichia coli, GI2367267, Length=474, Percent_Identity=83.5443037974684, Blast_Score=802, Evalue=0.0,
Organism=Escherichia coli, GI226510964, Length=230, Percent_Identity=26.9565217391304, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921 [H]

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C [H]

EC number: NA

Molecular weight: Translated: 53372; Mature: 53241

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEGIVSSSLWLQCLQRLQEELPAAEFSMWVRPLQAELNDNTLTLFAPNRFVLDWVRDKY
CCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHH
LNNINRLLMEFSGNDVPNLRFEVGSRPVVAPKPAPVRTAADVAAESSAPAQLAQRKPIHK
HHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHCCCCCC
TWDDDSAAADITHRSNVNPKHKFNNFVEGKSNQLGLAAARQVSDNPGAAYNPLFLYGGTG
CCCCCCCHHCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCEEEECCCC
LGKTHLLHAVGNAIVDNNPNAKVVYMHSERFVQDMVKALQNNAIEEFKRYYRSVDALLID
CCHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEISGVEDRLKSRFGWGLTVAIE
HHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEC
PPELETRVAILMKKAEDHQIHLPDEVAFFIAKRLRSNVRELEGALNRVIANANFTGRPIT
CCCHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
IDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVADLLSKRRSRSVARPRQLAMALAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
ELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREESHDIKEDYSNLIRTLSS
HHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEGIVSSSLWLQCLQRLQEELPAAEFSMWVRPLQAELNDNTLTLFAPNRFVLDWVRDKY
CCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHH
LNNINRLLMEFSGNDVPNLRFEVGSRPVVAPKPAPVRTAADVAAESSAPAQLAQRKPIHK
HHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHCCCCCC
TWDDDSAAADITHRSNVNPKHKFNNFVEGKSNQLGLAAARQVSDNPGAAYNPLFLYGGTG
CCCCCCCHHCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCEEEECCCC
LGKTHLLHAVGNAIVDNNPNAKVVYMHSERFVQDMVKALQNNAIEEFKRYYRSVDALLID
CCHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEISGVEDRLKSRFGWGLTVAIE
HHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEC
PPELETRVAILMKKAEDHQIHLPDEVAFFIAKRLRSNVRELEGALNRVIANANFTGRPIT
CCCHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
IDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVADLLSKRRSRSVARPRQLAMALAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
ELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREESHDIKEDYSNLIRTLSS
HHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA