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The map label for this gene is ldhA [C]

Identifier: 226950708

GI number: 226950708

Start: 3729817

End: 3730821

Strand: Direct

Name: ldhA [C]

Synonym: CLM_3716

Alternate gene names: 226950708

Gene position: 3729817-3730821 (Clockwise)

Preceding gene: 226950707

Following gene: 226950722

Centisome position: 89.76

GC content: 33.03

Gene sequence:

>1005_bases
ATGAAAATACTAATGTACAGCGTGCGTGAGCACGAAAAACCAGCTATAAAAAAATGGCTAGAAGCAAATCCTGGAGTTCA
AATTGATCTATGCAATGAGGCTCTTTCTGAAGATACTGTCTGTAAGGCAAAAGAATATGATGGAATTGCAATTCAGCAAA
CAAATAGTATAGGTGGAAAAGCTGTTTATTCTACCCTTAAAGAATATGGAATTAAACAAATAGCTTCAAGAACTGCTGGA
GTAGATATGATTGATTTAAAGACGGCTTCAGACAGCAATATTCTTGTTACCAATGTTCCTGCTTATTCTCCAAATGCTAT
TGCAGAACTTGCTGTAACTCATACAATGAATTTGTTGAGAAACATTAAAACTTTAAATAAAAGAATAGCTTATGGTGATT
ATCGTTGGTCAGCTGACCTTATAGCTAGAGAAGTAAGATCTGTAACAGTTGGTGTAGTAGGTACAGGCAAAATAGGTAGA
ACTTCTGCCAAGCTTTTTAAAGGATTAGGGGCAAATGTTATAGGTTATGATGCTTATCCAGACAAAAAATTAGAAGAAAA
TAATCTCCTTACTTACAAAGAATCATTGGATGATCTTTTAAGAGAAGCTGATGTTGTAACTCTGCACACACCTCTTCTAG
AAAGTACTAAACATATGATAAATAAGAATAATTTGAAATATATGAAACCAGATGCTTTCATAGTAAATACTGGTCGTGGT
GGAATTATCAACACTGAAGATCTTATTGAAGCCTTAGAACAAAATAAAATAGCTGGGGCTGCACTTGATACTTTTGAAAA
TGAAGGATTATTCTTAAATAAAGTAGTTGATCCAACTAAACTTCCTGACCCTCAACTTGATAAATTATTAAAAATGGATC
AAGTTTTAATAACTCATCATGTTGGTTTCTTCACTACTACTGCAGTTCAAAACATAGTTGATACCAGCTTAGATAGTGTT
GTAGAGGTTTTAAAGACTAACAATAGTGTAAACAAGGTAAATTAA

Upstream 100 bases:

>100_bases
CAAATTAATATTGTACATCAGAAGGCTTATGGCTTTTAATATATTTTTTATTAACTTACACTTTAAAAATATATTTAAAT
TCTAAAGGGGGAAATATTTT

Downstream 100 bases:

>100_bases
AAATTATCTTTTTCAAAAATGAAAGCGTTTTCAGGTTTTACTAATCTTAAAGTAAATAGAAAACGCTTTCTATTACCATT
TTATTATTTTCTTAAATTTT

Product: D-lactate dehydrogenase

Products: NA

Alternate protein names: D-LDH; D-specific D-2-hydroxyacid dehydrogenase [H]

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MKILMYSVREHEKPAIKKWLEANPGVQIDLCNEALSEDTVCKAKEYDGIAIQQTNSIGGKAVYSTLKEYGIKQIASRTAG
VDMIDLKTASDSNILVTNVPAYSPNAIAELAVTHTMNLLRNIKTLNKRIAYGDYRWSADLIAREVRSVTVGVVGTGKIGR
TSAKLFKGLGANVIGYDAYPDKKLEENNLLTYKESLDDLLREADVVTLHTPLLESTKHMINKNNLKYMKPDAFIVNTGRG
GIINTEDLIEALEQNKIAGAALDTFENEGLFLNKVVDPTKLPDPQLDKLLKMDQVLITHHVGFFTTTAVQNIVDTSLDSV
VEVLKTNNSVNKVN

Sequences:

>Translated_334_residues
MKILMYSVREHEKPAIKKWLEANPGVQIDLCNEALSEDTVCKAKEYDGIAIQQTNSIGGKAVYSTLKEYGIKQIASRTAG
VDMIDLKTASDSNILVTNVPAYSPNAIAELAVTHTMNLLRNIKTLNKRIAYGDYRWSADLIAREVRSVTVGVVGTGKIGR
TSAKLFKGLGANVIGYDAYPDKKLEENNLLTYKESLDDLLREADVVTLHTPLLESTKHMINKNNLKYMKPDAFIVNTGRG
GIINTEDLIEALEQNKIAGAALDTFENEGLFLNKVVDPTKLPDPQLDKLLKMDQVLITHHVGFFTTTAVQNIVDTSLDSV
VEVLKTNNSVNKVN
>Mature_334_residues
MKILMYSVREHEKPAIKKWLEANPGVQIDLCNEALSEDTVCKAKEYDGIAIQQTNSIGGKAVYSTLKEYGIKQIASRTAG
VDMIDLKTASDSNILVTNVPAYSPNAIAELAVTHTMNLLRNIKTLNKRIAYGDYRWSADLIAREVRSVTVGVVGTGKIGR
TSAKLFKGLGANVIGYDAYPDKKLEENNLLTYKESLDDLLREADVVTLHTPLLESTKHMINKNNLKYMKPDAFIVNTGRG
GIINTEDLIEALEQNKIAGAALDTFENEGLFLNKVVDPTKLPDPQLDKLLKMDQVLITHHVGFFTTTAVQNIVDTSLDSV
VEVLKTNNSVNKVN

Specific function: Fermentative Lactate Dehydrogenase. [C]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4557497, Length=248, Percent_Identity=33.8709677419355, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI61743967, Length=248, Percent_Identity=33.8709677419355, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI145580578, Length=248, Percent_Identity=31.0483870967742, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI4557499, Length=248, Percent_Identity=31.0483870967742, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI145580575, Length=196, Percent_Identity=34.1836734693878, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI23308577, Length=255, Percent_Identity=27.843137254902, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI6912396, Length=237, Percent_Identity=27.0042194092827, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1787645, Length=335, Percent_Identity=31.9402985074627, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1789279, Length=240, Percent_Identity=32.9166666666667, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI87082289, Length=269, Percent_Identity=27.1375464684015, Blast_Score=98, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17532191, Length=299, Percent_Identity=27.7591973244147, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI25147481, Length=276, Percent_Identity=25.3623188405797, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6322116, Length=319, Percent_Identity=28.2131661442006, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6320925, Length=319, Percent_Identity=27.2727272727273, Blast_Score=109, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6324055, Length=229, Percent_Identity=31.4410480349345, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324964, Length=261, Percent_Identity=28.3524904214559, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324980, Length=75, Percent_Identity=37.3333333333333, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24646446, Length=315, Percent_Identity=31.1111111111111, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24646448, Length=315, Percent_Identity=31.1111111111111, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24646452, Length=315, Percent_Identity=31.1111111111111, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24646450, Length=315, Percent_Identity=31.1111111111111, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI62472511, Length=269, Percent_Identity=33.457249070632, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI19921140, Length=240, Percent_Identity=31.25, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI28574286, Length=240, Percent_Identity=30, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI45552429, Length=242, Percent_Identity=32.2314049586777, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI28574284, Length=239, Percent_Identity=32.2175732217573, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI45551003, Length=242, Percent_Identity=32.2314049586777, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24585514, Length=242, Percent_Identity=32.2314049586777, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI28574282, Length=242, Percent_Identity=32.2314049586777, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI28571528, Length=234, Percent_Identity=27.7777777777778, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24585516, Length=268, Percent_Identity=23.5074626865672, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.28 [H]

Molecular weight: Translated: 36835; Mature: 36835

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILMYSVREHEKPAIKKWLEANPGVQIDLCNEALSEDTVCKAKEYDGIAIQQTNSIGGK
CEEEEEHHHHCCCHHHHHHHHCCCCEEEEECHHHHCCCCEECHHCCCCEEEEECCCCCHH
AVYSTLKEYGIKQIASRTAGVDMIDLKTASDSNILVTNVPAYSPNAIAELAVTHTMNLLR
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
NIKTLNKRIAYGDYRWSADLIAREVRSVTVGVVGTGKIGRTSAKLFKGLGANVIGYDAYP
HHHHHHHHHCCCCCCCHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHCCCCCEEEECCCC
DKKLEENNLLTYKESLDDLLREADVVTLHTPLLESTKHMINKNNLKYMKPDAFIVNTGRG
CCCCCCCCCEEHHHHHHHHHHHCCEEEEEHHHHHHHHHHHCCCCCEEECCCEEEEECCCC
GIINTEDLIEALEQNKIAGAALDTFENEGLFLNKVVDPTKLPDPQLDKLLKMDQVLITHH
CCCCHHHHHHHHHHCCCCHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
VGFFTTTAVQNIVDTSLDSVVEVLKTNNSVNKVN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKILMYSVREHEKPAIKKWLEANPGVQIDLCNEALSEDTVCKAKEYDGIAIQQTNSIGGK
CEEEEEHHHHCCCHHHHHHHHCCCCEEEEECHHHHCCCCEECHHCCCCEEEEECCCCCHH
AVYSTLKEYGIKQIASRTAGVDMIDLKTASDSNILVTNVPAYSPNAIAELAVTHTMNLLR
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
NIKTLNKRIAYGDYRWSADLIAREVRSVTVGVVGTGKIGRTSAKLFKGLGANVIGYDAYP
HHHHHHHHHCCCCCCCHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHCCCCCEEEECCCC
DKKLEENNLLTYKESLDDLLREADVVTLHTPLLESTKHMINKNNLKYMKPDAFIVNTGRG
CCCCCCCCCEEHHHHHHHHHHHCCEEEEEHHHHHHHHHHHCCCCCEEECCCEEEEECCCC
GIINTEDLIEALEQNKIAGAALDTFENEGLFLNKVVDPTKLPDPQLDKLLKMDQVLITHH
CCCCHHHHHHHHHHCCCCHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
VGFFTTTAVQNIVDTSLDSVVEVLKTNNSVNKVN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1840590; 8740366 [H]