Click here to switch to the map view.

The map label for this gene is thrS [H]

Identifier: 226950568

GI number: 226950568

Start: 3580114

End: 3582021

Strand: Reverse

Name: thrS [H]

Synonym: CLM_3546

Alternate gene names: 226950568

Gene position: 3582021-3580114 (Counterclockwise)

Preceding gene: 226950569

Following gene: 226950567

Centisome position: 86.2

GC content: 30.56

Gene sequence:

>1908_bases
ATGATAAAAATCACATTAAAAGATGGCAAAGTAATGGAATTTGAAGAAGGAATAAAGATATCTGATATTGCTATGAAAAT
TAGTCCAGCTCTTTATAAAAAAGCTTTAGCAGCTAAAATAGATGGAGAAACTGTGGATTTAATGACTGAGCTACACAAAG
ATAGTAGTTTAGAAATTTTAACATTTGAAGATGAAATGGGTAAATGGACTTTAAGACATACTGGCTCACATATGTTAGCG
CAAGCAGTTAAAAGATTATATCCAGAAGTAAAATTAGCTATAGGACCTGCTATAGACACTGGTTTTTATTATGATTTTGA
AGCAGATTTTACTTTTACACCAGAAATGTTAGAAAAAATAGAAGCAGAAATTAAAAAAATAATAAAAGAAAATCATAAGT
TAGAAAGATTTGAATTACCAAGAGAAGAAGCAATAAAGCTTATGAAAGAAAAGAATGAAGATTATAAGGTTGAGCTTATA
GAAGATTTACCAGAAGGAGAAGTTATATCTTTTTATAAACAAGGAGATTTTACAGATTTATGTGCAGGACCTCATGTACC
ATCTACAGGAAAGGTTAAATCAGTAAAATTGTTATCTCTAGCTGGAGCTTATTGGAGAGGCGATGAAAAAAATAAAATGC
TTCAAAGGATATATGGTACAGCCTTTACTAAAAAAAGTGAGCTAGACGAATATTTAAATATGATTGAAGAAGCTAAAAAA
AGAGATCATAGAAAATTGGGTAAAGAATTAGATTTATTTAGCATACATGAAGAGGGTCCAGGATTCCCATTTTTCCATCC
AAAGGGAATGATTGTAAGAAACATTCTTGAAAGTTTTTGGAGGGAAGAGCACACAAAAGCAGGATATCAAGAAATAAGGA
CACCATTAATATTAAATGAAGCTTTATGGCATCAATCAGGTCACTGGGATCACTACAAAGAAAATATGTATTTTACTAAT
ATAGATGATGGTGATTATGCAATAAAACCTATGAATTGTCCAGGTGGAATACTAGTCTATAAGAATTCTATGCATTCTTA
TAGAGATCTTCCATTAAGACTTTCAGAATTAGGAATAGTTCATAGACATGAATTATCAGGAGCATTACATGGACTTATGA
GAGTAAGATGTTTTACTCAAGATGATGCTCATTTATATATGACAAAAGAACAGATAAAAGAAGAAATAGTAGGAATAATT
AAATTAATAGATAAATTCTATAAACTATTTGGATTTGAATATTTTGTAGAGCTTTCAACAAGACCAGAGGATTCTATGGG
AAGTGATGAAGATTGGGAAATAGCAACTAATGGTTTAAGAGAAGCTTTAGATTCTATAGGAAAAGAATATAGAGTAAATG
AAGGTGACGGAGCTTTCTATGGTCCAAAGATAGATTTCCATTTAAAGGACTGTATAGGCAGAACATGGCAATGTGGAACT
ATTCAACTTGATTTCCAAATGCCAGAAAGATTTGATTTATCATATATAGGTGCAGATGGTGAAAAGCATAGACCTGTTAT
GGTTCATAGAACTATATATGGAAGTGTTGAAAGATTTATAGGTATATTAATAGAGCAATACGCAGGTGCATTCCCAACAT
GGCTTGCTCCAGTACAAGTTAAATTAATGAATATAACTGATTCTCAATATGATTATTTAAAGAAAGTTGAAGAAGCTCTT
AAAGAAAATAATATAAGGGTAGAAATTGATACAAGAAATGAAAAAATAGGGTATAAGATAAGAGAAGCTCAACTTCAAAA
AGTTCCATATATGCTAATACTTGGAGATAAAGAAGTGGAAGCAGGAAAAGTTGCTGTAAGAAGTAGAAAAGATGGAGATT
TAGGGGCTATATCCTTAGAAGAATTTATAGAAAAAATAAAGAATGAAATTAAGAACAAAACTAACTAA

Upstream 100 bases:

>100_bases
CACTGCTATTAAATTGGGTGGAACCACGAAAATACACTCGTCCCATGTGTTGGGACGGGTTTTTTTATTTGTAATTTTTT
ATTTGAAGGAGGACAATTTT

Downstream 100 bases:

>100_bases
AAATAAATTAATTTATAATAATAAAGGAATTTAAATTTTTTATAGAAAAATATTGACTTGCTAATACATCCATAGTATAA
TACTATTTGTTGATTAACTT

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 635; Mature: 635

Protein sequence:

>635_residues
MIKITLKDGKVMEFEEGIKISDIAMKISPALYKKALAAKIDGETVDLMTELHKDSSLEILTFEDEMGKWTLRHTGSHMLA
QAVKRLYPEVKLAIGPAIDTGFYYDFEADFTFTPEMLEKIEAEIKKIIKENHKLERFELPREEAIKLMKEKNEDYKVELI
EDLPEGEVISFYKQGDFTDLCAGPHVPSTGKVKSVKLLSLAGAYWRGDEKNKMLQRIYGTAFTKKSELDEYLNMIEEAKK
RDHRKLGKELDLFSIHEEGPGFPFFHPKGMIVRNILESFWREEHTKAGYQEIRTPLILNEALWHQSGHWDHYKENMYFTN
IDDGDYAIKPMNCPGGILVYKNSMHSYRDLPLRLSELGIVHRHELSGALHGLMRVRCFTQDDAHLYMTKEQIKEEIVGII
KLIDKFYKLFGFEYFVELSTRPEDSMGSDEDWEIATNGLREALDSIGKEYRVNEGDGAFYGPKIDFHLKDCIGRTWQCGT
IQLDFQMPERFDLSYIGADGEKHRPVMVHRTIYGSVERFIGILIEQYAGAFPTWLAPVQVKLMNITDSQYDYLKKVEEAL
KENNIRVEIDTRNEKIGYKIREAQLQKVPYMLILGDKEVEAGKVAVRSRKDGDLGAISLEEFIEKIKNEIKNKTN

Sequences:

>Translated_635_residues
MIKITLKDGKVMEFEEGIKISDIAMKISPALYKKALAAKIDGETVDLMTELHKDSSLEILTFEDEMGKWTLRHTGSHMLA
QAVKRLYPEVKLAIGPAIDTGFYYDFEADFTFTPEMLEKIEAEIKKIIKENHKLERFELPREEAIKLMKEKNEDYKVELI
EDLPEGEVISFYKQGDFTDLCAGPHVPSTGKVKSVKLLSLAGAYWRGDEKNKMLQRIYGTAFTKKSELDEYLNMIEEAKK
RDHRKLGKELDLFSIHEEGPGFPFFHPKGMIVRNILESFWREEHTKAGYQEIRTPLILNEALWHQSGHWDHYKENMYFTN
IDDGDYAIKPMNCPGGILVYKNSMHSYRDLPLRLSELGIVHRHELSGALHGLMRVRCFTQDDAHLYMTKEQIKEEIVGII
KLIDKFYKLFGFEYFVELSTRPEDSMGSDEDWEIATNGLREALDSIGKEYRVNEGDGAFYGPKIDFHLKDCIGRTWQCGT
IQLDFQMPERFDLSYIGADGEKHRPVMVHRTIYGSVERFIGILIEQYAGAFPTWLAPVQVKLMNITDSQYDYLKKVEEAL
KENNIRVEIDTRNEKIGYKIREAQLQKVPYMLILGDKEVEAGKVAVRSRKDGDLGAISLEEFIEKIKNEIKNKTN
>Mature_635_residues
MIKITLKDGKVMEFEEGIKISDIAMKISPALYKKALAAKIDGETVDLMTELHKDSSLEILTFEDEMGKWTLRHTGSHMLA
QAVKRLYPEVKLAIGPAIDTGFYYDFEADFTFTPEMLEKIEAEIKKIIKENHKLERFELPREEAIKLMKEKNEDYKVELI
EDLPEGEVISFYKQGDFTDLCAGPHVPSTGKVKSVKLLSLAGAYWRGDEKNKMLQRIYGTAFTKKSELDEYLNMIEEAKK
RDHRKLGKELDLFSIHEEGPGFPFFHPKGMIVRNILESFWREEHTKAGYQEIRTPLILNEALWHQSGHWDHYKENMYFTN
IDDGDYAIKPMNCPGGILVYKNSMHSYRDLPLRLSELGIVHRHELSGALHGLMRVRCFTQDDAHLYMTKEQIKEEIVGII
KLIDKFYKLFGFEYFVELSTRPEDSMGSDEDWEIATNGLREALDSIGKEYRVNEGDGAFYGPKIDFHLKDCIGRTWQCGT
IQLDFQMPERFDLSYIGADGEKHRPVMVHRTIYGSVERFIGILIEQYAGAFPTWLAPVQVKLMNITDSQYDYLKKVEEAL
KENNIRVEIDTRNEKIGYKIREAQLQKVPYMLILGDKEVEAGKVAVRSRKDGDLGAISLEEFIEKIKNEIKNKTN

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38202255, Length=630, Percent_Identity=41.9047619047619, Blast_Score=508, Evalue=1e-144,
Organism=Homo sapiens, GI38570111, Length=638, Percent_Identity=40.9090909090909, Blast_Score=503, Evalue=1e-142,
Organism=Homo sapiens, GI20070344, Length=652, Percent_Identity=37.5766871165644, Blast_Score=447, Evalue=1e-125,
Organism=Escherichia coli, GI1788013, Length=636, Percent_Identity=48.2704402515723, Blast_Score=653, Evalue=0.0,
Organism=Escherichia coli, GI1786392, Length=202, Percent_Identity=25.2475247524752, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71984184, Length=630, Percent_Identity=40.6349206349206, Blast_Score=512, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI71984192, Length=630, Percent_Identity=40.6349206349206, Blast_Score=511, Evalue=1e-145,
Organism=Saccharomyces cerevisiae, GI6322112, Length=651, Percent_Identity=38.5560675883257, Blast_Score=478, Evalue=1e-135,
Organism=Saccharomyces cerevisiae, GI6322654, Length=397, Percent_Identity=39.0428211586902, Blast_Score=301, Evalue=3e-82,
Organism=Drosophila melanogaster, GI24583842, Length=629, Percent_Identity=40.8585055643879, Blast_Score=511, Evalue=1e-145,
Organism=Drosophila melanogaster, GI28574141, Length=629, Percent_Identity=40.8585055643879, Blast_Score=511, Evalue=1e-145,
Organism=Drosophila melanogaster, GI24583839, Length=630, Percent_Identity=41.1111111111111, Blast_Score=510, Evalue=1e-144,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 73394; Mature: 73394

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKITLKDGKVMEFEEGIKISDIAMKISPALYKKALAAKIDGETVDLMTELHKDSSLEIL
CEEEEECCCCEEEHHCCCEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEE
TFEDEMGKWTLRHTGSHMLAQAVKRLYPEVKLAIGPAIDTGFYYDFEADFTFTPEMLEKI
EEECCCCCEEEHHCCHHHHHHHHHHHCCCEEEEECCEECCCEEEEECCCCEECHHHHHHH
EAEIKKIIKENHKLERFELPREEAIKLMKEKNEDYKVELIEDLPEGEVISFYKQGDFTDL
HHHHHHHHHHCCCCHHHCCCHHHHHHHHHHCCCCCEEEHHHCCCCCCEEEEEECCCCCHH
CAGPHVPSTGKVKSVKLLSLAGAYWRGDEKNKMLQRIYGTAFTKKSELDEYLNMIEEAKK
CCCCCCCCCCCEEEEEHEEHHCHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RDHRKLGKELDLFSIHEEGPGFPFFHPKGMIVRNILESFWREEHTKAGYQEIRTPLILNE
HHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHH
ALWHQSGHWDHYKENMYFTNIDDGDYAIKPMNCPGGILVYKNSMHSYRDLPLRLSELGIV
HHHCCCCCCCCHHCCEEEEECCCCCEEEEECCCCCCEEEEECCCCCHHCCCCCHHHHCCH
HRHELSGALHGLMRVRCFTQDDAHLYMTKEQIKEEIVGIIKLIDKFYKLFGFEYFVELST
HHHHHHHHHHHHHHHEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECC
RPEDSMGSDEDWEIATNGLREALDSIGKEYRVNEGDGAFYGPKIDFHLKDCIGRTWQCGT
CCCCCCCCCCCHHHHHHHHHHHHHHCCHHEECCCCCCCEECCEEEEEHHHHCCCCEECCE
IQLDFQMPERFDLSYIGADGEKHRPVMVHRTIYGSVERFIGILIEQYAGAFPTWLAPVQV
EEEEEECCCCCCEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
KLMNITDSQYDYLKKVEEALKENNIRVEIDTRNEKIGYKIREAQLQKVPYMLILGDKEVE
EEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHCCEEEEECCCCCC
AGKVAVRSRKDGDLGAISLEEFIEKIKNEIKNKTN
CCCEEEECCCCCCCCEECHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIKITLKDGKVMEFEEGIKISDIAMKISPALYKKALAAKIDGETVDLMTELHKDSSLEIL
CEEEEECCCCEEEHHCCCEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEE
TFEDEMGKWTLRHTGSHMLAQAVKRLYPEVKLAIGPAIDTGFYYDFEADFTFTPEMLEKI
EEECCCCCEEEHHCCHHHHHHHHHHHCCCEEEEECCEECCCEEEEECCCCEECHHHHHHH
EAEIKKIIKENHKLERFELPREEAIKLMKEKNEDYKVELIEDLPEGEVISFYKQGDFTDL
HHHHHHHHHHCCCCHHHCCCHHHHHHHHHHCCCCCEEEHHHCCCCCCEEEEEECCCCCHH
CAGPHVPSTGKVKSVKLLSLAGAYWRGDEKNKMLQRIYGTAFTKKSELDEYLNMIEEAKK
CCCCCCCCCCCEEEEEHEEHHCHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RDHRKLGKELDLFSIHEEGPGFPFFHPKGMIVRNILESFWREEHTKAGYQEIRTPLILNE
HHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHH
ALWHQSGHWDHYKENMYFTNIDDGDYAIKPMNCPGGILVYKNSMHSYRDLPLRLSELGIV
HHHCCCCCCCCHHCCEEEEECCCCCEEEEECCCCCCEEEEECCCCCHHCCCCCHHHHCCH
HRHELSGALHGLMRVRCFTQDDAHLYMTKEQIKEEIVGIIKLIDKFYKLFGFEYFVELST
HHHHHHHHHHHHHHHEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECC
RPEDSMGSDEDWEIATNGLREALDSIGKEYRVNEGDGAFYGPKIDFHLKDCIGRTWQCGT
CCCCCCCCCCCHHHHHHHHHHHHHHCCHHEECCCCCCCEECCEEEEEHHHHCCCCEECCE
IQLDFQMPERFDLSYIGADGEKHRPVMVHRTIYGSVERFIGILIEQYAGAFPTWLAPVQV
EEEEEECCCCCCEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
KLMNITDSQYDYLKKVEEALKENNIRVEIDTRNEKIGYKIREAQLQKVPYMLILGDKEVE
EEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHCCEEEEECCCCCC
AGKVAVRSRKDGDLGAISLEEFIEKIKNEIKNKTN
CCCEEEECCCCCCCCEECHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA