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The map label for this gene is queA [H]

Identifier: 226950498

GI number: 226950498

Start: 3495153

End: 3496178

Strand: Reverse

Name: queA [H]

Synonym: CLM_3474

Alternate gene names: 226950498

Gene position: 3496178-3495153 (Counterclockwise)

Preceding gene: 226950499

Following gene: 226950497

Centisome position: 84.14

GC content: 32.07

Gene sequence:

>1026_bases
GTGAAAGTAAAAGATTTTGATTTTTATTTACCAGAGGAATTAATAGCACAACATCCTATGGAGAAAAGAGATGAAGCTAG
ATTACTTGTATTAGATAAGAAAACAGGTGGAATAGAACATAAAATATTTAAGGATATTTTAGACTATTTAACACCAAATG
ATTGCTTAGTTTTAAATAATACTAGAGTTTTACCTGCAAGACTTATAGGGGCGAAAGAAGAAACCGGTGGGAAAATGGAA
TTTTTACTTTTAAAAAGAAAAGAAAAAGATGTATGGGAAACTTTAGTTAAGCCAGGAAAGAGGGCCCAGATTGGGGCAAG
GTTCATATTTGGTAATGGAGAGCTTAAAGCAGAAGTCATAGGAATGGGAGAAGAAGGAAGTAGAATAGTAAAATTTTATT
ATGAAGGTATTTTCGAGGAAATATTAGATCAACTAGGACAAATGCCCCTACCTCCATATATAAAGGAAAAGCTAGATGAT
AAGGAGATGTATCAAACAGTTTATTCAAAGGAAGAGGGATCGGCAGCAGCTCCTACAGCAGGACTTCATTTTACAGAGGA
ACTTTTGAAAAAAATAAAAGAAAAAGGTGTGAAATTAGCCTTTTTAACCTTACATGTAGGATTAGGAACTTTTAGACCAG
TTAAAGTAGAAAATATTCAAGAGCATGTTATGCATTCAGAATATTATAAGATGGATAAAGAAACTGCTGAAATAATAAAT
GATACAAAAGAAAAAGGTGGAAGAGTTATAGCAGTAGGAACTACTTCCTGTAGAACATTGGAAACTATAGGAGACATAGA
AGGTAAAGTAAGGGAACAATCAGGATGGACAGATATATTTATTTATCCCGGATATAAATACAAAGTAGTGGATGCTTTAA
TTACTAATTTTCATCTTCCACAATCTACATTATTAATGTTAGTATCTGCATTAGCAGGAAGAGACAATATAATGAATGCA
TATAATGTGGCAGTAGAAAAGGAATATAGATTCTTCTCTTTTGGAGATGCTATGTTTATTAAATAA

Upstream 100 bases:

>100_bases
ATATAACCATTTTGGAGTAACAAAAAAAGAAGAAAAGGACAATCAAGTATCTATATTTAATAAATAATAAATTTTAATTC
TATATTAAGAGGTGGAAGAC

Downstream 100 bases:

>100_bases
TATTATTAAGTAATTTTAATAAAAGCTTAATCCCTAAGAAAAAAGAGGTGTAAGTATGTACAAATTGTTAAAAAAGTCAG
GAAAAGCAAGGCGAGGAGAA

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MKVKDFDFYLPEELIAQHPMEKRDEARLLVLDKKTGGIEHKIFKDILDYLTPNDCLVLNNTRVLPARLIGAKEETGGKME
FLLLKRKEKDVWETLVKPGKRAQIGARFIFGNGELKAEVIGMGEEGSRIVKFYYEGIFEEILDQLGQMPLPPYIKEKLDD
KEMYQTVYSKEEGSAAAPTAGLHFTEELLKKIKEKGVKLAFLTLHVGLGTFRPVKVENIQEHVMHSEYYKMDKETAEIIN
DTKEKGGRVIAVGTTSCRTLETIGDIEGKVREQSGWTDIFIYPGYKYKVVDALITNFHLPQSTLLMLVSALAGRDNIMNA
YNVAVEKEYRFFSFGDAMFIK

Sequences:

>Translated_341_residues
MKVKDFDFYLPEELIAQHPMEKRDEARLLVLDKKTGGIEHKIFKDILDYLTPNDCLVLNNTRVLPARLIGAKEETGGKME
FLLLKRKEKDVWETLVKPGKRAQIGARFIFGNGELKAEVIGMGEEGSRIVKFYYEGIFEEILDQLGQMPLPPYIKEKLDD
KEMYQTVYSKEEGSAAAPTAGLHFTEELLKKIKEKGVKLAFLTLHVGLGTFRPVKVENIQEHVMHSEYYKMDKETAEIIN
DTKEKGGRVIAVGTTSCRTLETIGDIEGKVREQSGWTDIFIYPGYKYKVVDALITNFHLPQSTLLMLVSALAGRDNIMNA
YNVAVEKEYRFFSFGDAMFIK
>Mature_341_residues
MKVKDFDFYLPEELIAQHPMEKRDEARLLVLDKKTGGIEHKIFKDILDYLTPNDCLVLNNTRVLPARLIGAKEETGGKME
FLLLKRKEKDVWETLVKPGKRAQIGARFIFGNGELKAEVIGMGEEGSRIVKFYYEGIFEEILDQLGQMPLPPYIKEKLDD
KEMYQTVYSKEEGSAAAPTAGLHFTEELLKKIKEKGVKLAFLTLHVGLGTFRPVKVENIQEHVMHSEYYKMDKETAEIIN
DTKEKGGRVIAVGTTSCRTLETIGDIEGKVREQSGWTDIFIYPGYKYKVVDALITNFHLPQSTLLMLVSALAGRDNIMNA
YNVAVEKEYRFFSFGDAMFIK

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=344, Percent_Identity=50, Blast_Score=338, Evalue=3e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38850; Mature: 38850

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVKDFDFYLPEELIAQHPMEKRDEARLLVLDKKTGGIEHKIFKDILDYLTPNDCLVLNN
CCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEECC
TRVLPARLIGAKEETGGKMEFLLLKRKEKDVWETLVKPGKRAQIGARFIFGNGELKAEVI
CEECHHHHHCCCCCCCCCEEEEEEECCHHHHHHHHHCCCCCCEECEEEEEECCCEEEEEE
GMGEEGSRIVKFYYEGIFEEILDQLGQMPLPPYIKEKLDDKEMYQTVYSKEEGSAAAPTA
ECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHCCCCCCCCCCCC
GLHFTEELLKKIKEKGVKLAFLTLHVGLGTFRPVKVENIQEHVMHSEYYKMDKETAEIIN
CHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
DTKEKGGRVIAVGTTSCRTLETIGDIEGKVREQSGWTDIFIYPGYKYKVVDALITNFHLP
HHHHHCCEEEEEECCCCHHHHHHHHCCCCEECCCCCEEEEEECCCCHHHHHHHHHHCCCC
QSTLLMLVSALAGRDNIMNAYNVAVEKEYRFFSFGDAMFIK
HHHHHHHHHHHCCCCHHHHHHHEEEECCEEEEEECCEEEEC
>Mature Secondary Structure
MKVKDFDFYLPEELIAQHPMEKRDEARLLVLDKKTGGIEHKIFKDILDYLTPNDCLVLNN
CCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEECC
TRVLPARLIGAKEETGGKMEFLLLKRKEKDVWETLVKPGKRAQIGARFIFGNGELKAEVI
CEECHHHHHCCCCCCCCCEEEEEEECCHHHHHHHHHCCCCCCEECEEEEEECCCEEEEEE
GMGEEGSRIVKFYYEGIFEEILDQLGQMPLPPYIKEKLDDKEMYQTVYSKEEGSAAAPTA
ECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHCCCCCCCCCCCC
GLHFTEELLKKIKEKGVKLAFLTLHVGLGTFRPVKVENIQEHVMHSEYYKMDKETAEIIN
CHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
DTKEKGGRVIAVGTTSCRTLETIGDIEGKVREQSGWTDIFIYPGYKYKVVDALITNFHLP
HHHHHCCEEEEEECCCCHHHHHHHHCCCCEECCCCCEEEEEECCCCHHHHHHHHHHCCCC
QSTLLMLVSALAGRDNIMNAYNVAVEKEYRFFSFGDAMFIK
HHHHHHHHHHHCCCCHHHHHHHEEEECCEEEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA