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The map label for this gene is tgt [H]

Identifier: 226950497

GI number: 226950497

Start: 3493967

End: 3495097

Strand: Reverse

Name: tgt [H]

Synonym: CLM_3473

Alternate gene names: 226950497

Gene position: 3495097-3493967 (Counterclockwise)

Preceding gene: 226950498

Following gene: 226950496

Centisome position: 84.11

GC content: 33.16

Gene sequence:

>1131_bases
ATGTACAAATTGTTAAAAAAGTCAGGAAAAGCAAGGCGAGGAGAATTTACAACTCCTCATGGGGTGATACAGACTCCTGT
ATTTATGAATGTGGGAACTCTTGCTGCCATAAAAGGGGCAGTATCATCTATGGATTTAAAGGAAATAGGATGTCAAGTTG
AGCTATCTAATACATACCATCTTCATTTAAGACCAGGAGATGAAGTTGTTAAAAAAATGGGTGGATTACATAAATTTATG
AATTGGGATAGGCCAATACTTACAGATTCAGGTGGATTTCAGGTATTTTCATTATCAAAAATAAGAAAAATACAAGAAGA
AGGAGTTTATTTTAATTCTCATATAGATGGAAGAAAAATATTTATGGGGCCGGAAGAAAGTATGAGGATACAAAGCAATC
TAGCTTCAACTATAGCTATGGCTTTTGATGAATGTGTAGAAAATCCAGCACCAAGAGAGTATGTAGAAAAGTCCGTAGAA
AGAACAACGAGATGGTTACATAGATGTAAGGATGAAATGAATAGATTAAACTCTTTGCCGGATACTATAAATAATAAGCA
AATGTTATTTGGTATAAATCAAGGTGGAACTTATGAAGATATAAGAATAGAACATGCTAAAACTATAGCTAAAATGGATC
TAGATGGATATGCTATAGGAGGATTAGCTGTTGGAGAAAGTCATGAAGATATGTATAGAATAATAGATGCAGTGGTTCCT
CACCTTCCAGAAGATAAACCTATATATCTTATGGGAGTTGGAATACCAAGTAATATATTAGAAGCTGTAGATAGGGGTGT
AGATTTCTTTGACTGTGTTCTTCCTGCAAGAAATGGAAGACATGCACATGTTTTCACAAAAGAGGGCAAAATAAATTTAT
TGAATGCAAAATTTGAATTAGATGATAGACCTATTGATGAAGGCTGTCAGTGTCCAGCTTGTAAGCATTATACAAGGTCA
TATATAAGGCATTTATTTAAAGCAAAGGAAATGCTAGCTATGAGATTATGTGTTTTACATAACTTATATTTCTATAATAA
CTTAATGGAAGAAATAAGAGATGCCATTGATGGAGATTATTTTAAAGAATACAAAGAAAGAAAACTTAAGGAGTGGGGAG
GAAGAGCATAA

Upstream 100 bases:

>100_bases
GAATATAGATTCTTCTCTTTTGGAGATGCTATGTTTATTAAATAATATTATTAAGTAATTTTAATAAAAGCTTAATCCCT
AAGAAAAAAGAGGTGTAAGT

Downstream 100 bases:

>100_bases
AGAATTAAAAATAATCTTTGTGTATTAAATGTTATAATAAATTTTATATTTTAAATATAAAATAATATAAATATATCTAT
TAATTGTAAATTATCAATTA

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MYKLLKKSGKARRGEFTTPHGVIQTPVFMNVGTLAAIKGAVSSMDLKEIGCQVELSNTYHLHLRPGDEVVKKMGGLHKFM
NWDRPILTDSGGFQVFSLSKIRKIQEEGVYFNSHIDGRKIFMGPEESMRIQSNLASTIAMAFDECVENPAPREYVEKSVE
RTTRWLHRCKDEMNRLNSLPDTINNKQMLFGINQGGTYEDIRIEHAKTIAKMDLDGYAIGGLAVGESHEDMYRIIDAVVP
HLPEDKPIYLMGVGIPSNILEAVDRGVDFFDCVLPARNGRHAHVFTKEGKINLLNAKFELDDRPIDEGCQCPACKHYTRS
YIRHLFKAKEMLAMRLCVLHNLYFYNNLMEEIRDAIDGDYFKEYKERKLKEWGGRA

Sequences:

>Translated_376_residues
MYKLLKKSGKARRGEFTTPHGVIQTPVFMNVGTLAAIKGAVSSMDLKEIGCQVELSNTYHLHLRPGDEVVKKMGGLHKFM
NWDRPILTDSGGFQVFSLSKIRKIQEEGVYFNSHIDGRKIFMGPEESMRIQSNLASTIAMAFDECVENPAPREYVEKSVE
RTTRWLHRCKDEMNRLNSLPDTINNKQMLFGINQGGTYEDIRIEHAKTIAKMDLDGYAIGGLAVGESHEDMYRIIDAVVP
HLPEDKPIYLMGVGIPSNILEAVDRGVDFFDCVLPARNGRHAHVFTKEGKINLLNAKFELDDRPIDEGCQCPACKHYTRS
YIRHLFKAKEMLAMRLCVLHNLYFYNNLMEEIRDAIDGDYFKEYKERKLKEWGGRA
>Mature_376_residues
MYKLLKKSGKARRGEFTTPHGVIQTPVFMNVGTLAAIKGAVSSMDLKEIGCQVELSNTYHLHLRPGDEVVKKMGGLHKFM
NWDRPILTDSGGFQVFSLSKIRKIQEEGVYFNSHIDGRKIFMGPEESMRIQSNLASTIAMAFDECVENPAPREYVEKSVE
RTTRWLHRCKDEMNRLNSLPDTINNKQMLFGINQGGTYEDIRIEHAKTIAKMDLDGYAIGGLAVGESHEDMYRIIDAVVP
HLPEDKPIYLMGVGIPSNILEAVDRGVDFFDCVLPARNGRHAHVFTKEGKINLLNAKFELDDRPIDEGCQCPACKHYTRS
YIRHLFKAKEMLAMRLCVLHNLYFYNNLMEEIRDAIDGDYFKEYKERKLKEWGGRA

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=355, Percent_Identity=40.8450704225352, Blast_Score=288, Evalue=4e-78,
Organism=Homo sapiens, GI13375872, Length=254, Percent_Identity=27.9527559055118, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI1786607, Length=356, Percent_Identity=47.4719101123596, Blast_Score=356, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI17544680, Length=374, Percent_Identity=40.1069518716578, Blast_Score=274, Evalue=5e-74,
Organism=Caenorhabditis elegans, GI25146502, Length=138, Percent_Identity=28.2608695652174, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19920510, Length=365, Percent_Identity=41.3698630136986, Blast_Score=293, Evalue=1e-79,
Organism=Drosophila melanogaster, GI21355311, Length=228, Percent_Identity=26.3157894736842, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42941; Mature: 42941

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKLLKKSGKARRGEFTTPHGVIQTPVFMNVGTLAAIKGAVSSMDLKEIGCQVELSNTYH
CCHHHHHCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHCCCHHHCCCEEEECCEEE
LHLRPGDEVVKKMGGLHKFMNWDRPILTDSGGFQVFSLSKIRKIQEEGVYFNSHIDGRKI
EEECCHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEHHHHHHHHHCCCEEECCCCCCEE
FMGPEESMRIQSNLASTIAMAFDECVENPAPREYVEKSVERTTRWLHRCKDEMNRLNSLP
EECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DTINNKQMLFGINQGGTYEDIRIEHAKTIAKMDLDGYAIGGLAVGESHEDMYRIIDAVVP
CCCCCCEEEEEECCCCCCHHEEHHHHHHHHHCCCCCEEECCEEECCCHHHHHHHHHHHHC
HLPEDKPIYLMGVGIPSNILEAVDRGVDFFDCVLPARNGRHAHVFTKEGKINLLNAKFEL
CCCCCCCEEEEECCCCHHHHHHHHCCCCHHHHHCCCCCCCEEEEEEECCCEEEEEEEEEC
DDRPIDEGCQCPACKHYTRSYIRHLFKAKEMLAMRLCVLHNLYFYNNLMEEIRDAIDGDY
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
FKEYKERKLKEWGGRA
HHHHHHHHHHHCCCCC
>Mature Secondary Structure
MYKLLKKSGKARRGEFTTPHGVIQTPVFMNVGTLAAIKGAVSSMDLKEIGCQVELSNTYH
CCHHHHHCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHCCCHHHCCCEEEECCEEE
LHLRPGDEVVKKMGGLHKFMNWDRPILTDSGGFQVFSLSKIRKIQEEGVYFNSHIDGRKI
EEECCHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEHHHHHHHHHCCCEEECCCCCCEE
FMGPEESMRIQSNLASTIAMAFDECVENPAPREYVEKSVERTTRWLHRCKDEMNRLNSLP
EECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DTINNKQMLFGINQGGTYEDIRIEHAKTIAKMDLDGYAIGGLAVGESHEDMYRIIDAVVP
CCCCCCEEEEEECCCCCCHHEEHHHHHHHHHCCCCCEEECCEEECCCHHHHHHHHHHHHC
HLPEDKPIYLMGVGIPSNILEAVDRGVDFFDCVLPARNGRHAHVFTKEGKINLLNAKFEL
CCCCCCCEEEEECCCCHHHHHHHHCCCCHHHHHCCCCCCCEEEEEEECCCEEEEEEEEEC
DDRPIDEGCQCPACKHYTRSYIRHLFKAKEMLAMRLCVLHNLYFYNNLMEEIRDAIDGDY
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
FKEYKERKLKEWGGRA
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA