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The map label for this gene is manZ [H]

Identifier: 226950259

GI number: 226950259

Start: 3240392

End: 3241336

Strand: Direct

Name: manZ [H]

Synonym: CLM_3216

Alternate gene names: 226950259

Gene position: 3240392-3241336 (Clockwise)

Preceding gene: 226950258

Following gene: 226950260

Centisome position: 77.98

GC content: 35.98

Gene sequence:

>945_bases
TTGATAGATATTAAGAAGGGAGAGGACATGAAAATGGCAAAAGAATTAAAATTAACAAAAAAAGATCGTATTTCTGTTTG
GTTGCGTTCATTTTTCCTTCAAGGTTCTTGGAACTATGAAAGAATGCAAAACGGTGGTTGGGCATTCCCAATGATTCCTG
CAATCAAAAAATTATACAAGACTAAAGAAGATCGAGCAGATGCATTGAAACGTCACTTGGAGTTCTTTAACACTCACCCA
TATGTAGCTTCACCAGTTCTTGGGGTAACATTAGCTTTAGAAGAACAACGTGCAAATGGTGCAGAAATCGATGATATAAC
TATTCAAGGTGTTAAAGTTGGTATGATGGGACCTTTAGCTGGTATTGGAGATCCAGTTTTTTGGTTTACTGTTAGGTCAA
TTTTAGGGGCATTAGCTGCTTCACTTGCTATGAATGGTAATATCCTAGGACCATTAATCTTTTTCTTGGCTTGGAATATC
ATCCGTATGGGATTTATGTGGTATACACAAGAGTTCGGTTTCAAAACAGAATCTCGTATTGCTGATGATTTATCAGGTGG
TTTACTACAAGATATTACAAAAGGAGCATCCATCCTTGGTATGTTCATTTTAGGAGCATTAGTTAACAGATGGGTATCCG
TTAAATTTACACCAGTAGTATCATCCGTTAAGTTAAGTGATGGTGCTTTCATTGATTGGAGCAAACTTCCTAAAGGAGCG
AAAGGTATTCAACAAGCTCTTTTACAACAAGGATCTGGTATGTCATTAACTGATTATAAAGTTACAACATTCCAAGATAG
CTTGGATTCATTAATTCCTGGACTTGCAGGATTAATAATTACATTTATTTGTGCGTGGTTACTTAAGAAGAAAGTATCTC
CAATTACCATTATCCTTGGGCTATTTGCACTGGGTATTGTTTTCCACTTAATCGGTTTAATGTAA

Upstream 100 bases:

>100_bases
ATAGGAGTAGCTTTAGCTCTTGTTTACTTAGCACTTTGTAAACAAGGTGGCTTAGGTAATGGTGAAAGCTCAAATACTGG
TGATCCTTTAGGGGATATTA

Downstream 100 bases:

>100_bases
CATTTTTTATTTAGCCTGGGCTTTTGTTAACATATAAAGCAGTTAAAGAATGAAACAAATTGATGAAATATGTGTTATTT
TAATGGACTTTTAAACAATT

Product: PTS system mannose/fructose/sorbose family transporter subunit IID

Products: protein histidine; sugar phosphate; D-glucosamine-6-phosphate [Cytoplasm]; pyruvate; glucose-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EII-M-Man; EIID-Man; PTS system mannose-specific EIID component [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MIDIKKGEDMKMAKELKLTKKDRISVWLRSFFLQGSWNYERMQNGGWAFPMIPAIKKLYKTKEDRADALKRHLEFFNTHP
YVASPVLGVTLALEEQRANGAEIDDITIQGVKVGMMGPLAGIGDPVFWFTVRSILGALAASLAMNGNILGPLIFFLAWNI
IRMGFMWYTQEFGFKTESRIADDLSGGLLQDITKGASILGMFILGALVNRWVSVKFTPVVSSVKLSDGAFIDWSKLPKGA
KGIQQALLQQGSGMSLTDYKVTTFQDSLDSLIPGLAGLIITFICAWLLKKKVSPITIILGLFALGIVFHLIGLM

Sequences:

>Translated_314_residues
MIDIKKGEDMKMAKELKLTKKDRISVWLRSFFLQGSWNYERMQNGGWAFPMIPAIKKLYKTKEDRADALKRHLEFFNTHP
YVASPVLGVTLALEEQRANGAEIDDITIQGVKVGMMGPLAGIGDPVFWFTVRSILGALAASLAMNGNILGPLIFFLAWNI
IRMGFMWYTQEFGFKTESRIADDLSGGLLQDITKGASILGMFILGALVNRWVSVKFTPVVSSVKLSDGAFIDWSKLPKGA
KGIQQALLQQGSGMSLTDYKVTTFQDSLDSLIPGLAGLIITFICAWLLKKKVSPITIILGLFALGIVFHLIGLM
>Mature_314_residues
MIDIKKGEDMKMAKELKLTKKDRISVWLRSFFLQGSWNYERMQNGGWAFPMIPAIKKLYKTKEDRADALKRHLEFFNTHP
YVASPVLGVTLALEEQRANGAEIDDITIQGVKVGMMGPLAGIGDPVFWFTVRSILGALAASLAMNGNILGPLIFFLAWNI
IRMGFMWYTQEFGFKTESRIADDLSGGLLQDITKGASILGMFILGALVNRWVSVKFTPVVSSVKLSDGAFIDWSKLPKGA
KGIQQALLQQGSGMSLTDYKVTTFQDSLDSLIPGLAGLIITFICAWLLKKKVSPITIILGLFALGIVFHLIGLM

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3716

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IID

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIID domain [H]

Homologues:

Organism=Escherichia coli, GI1788122, Length=308, Percent_Identity=48.3766233766234, Blast_Score=298, Evalue=4e-82,
Organism=Escherichia coli, GI1789529, Length=303, Percent_Identity=31.3531353135314, Blast_Score=149, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004704
- InterPro:   IPR018405 [H]

Pfam domain/function: PF03613 EIID-AGA [H]

EC number: NA

Molecular weight: Translated: 34714; Mature: 34714

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: PS51108 PTS_EIID

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDIKKGEDMKMAKELKLTKKDRISVWLRSFFLQGSWNYERMQNGGWAFPMIPAIKKLYK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHH
TKEDRADALKRHLEFFNTHPYVASPVLGVTLALEEQRANGAEIDDITIQGVKVGMMGPLA
CCHHHHHHHHHHHHHHCCCCCHHCHHHHHHEEEEHHCCCCCCCCCEEECCEEECCCCCCC
GIGDPVFWFTVRSILGALAASLAMNGNILGPLIFFLAWNIIRMGFMWYTQEFGFKTESRI
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ADDLSGGLLQDITKGASILGMFILGALVNRWVSVKFTPVVSSVKLSDGAFIDWSKLPKGA
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHEEEECCCCEEEHHHCCCCH
KGIQQALLQQGSGMSLTDYKVTTFQDSLDSLIPGLAGLIITFICAWLLKKKVSPITIILG
HHHHHHHHHCCCCCCEECEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LFALGIVFHLIGLM
HHHHHHHHHHHHCC
>Mature Secondary Structure
MIDIKKGEDMKMAKELKLTKKDRISVWLRSFFLQGSWNYERMQNGGWAFPMIPAIKKLYK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHH
TKEDRADALKRHLEFFNTHPYVASPVLGVTLALEEQRANGAEIDDITIQGVKVGMMGPLA
CCHHHHHHHHHHHHHHCCCCCHHCHHHHHHEEEEHHCCCCCCCCCEEECCEEECCCCCCC
GIGDPVFWFTVRSILGALAASLAMNGNILGPLIFFLAWNIIRMGFMWYTQEFGFKTESRI
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ADDLSGGLLQDITKGASILGMFILGALVNRWVSVKFTPVVSSVKLSDGAFIDWSKLPKGA
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHEEEECCCCEEEHHHCCCCH
KGIQQALLQQGSGMSLTDYKVTTFQDSLDSLIPGLAGLIITFICAWLLKKKVSPITIILG
HHHHHHHHHCCCCCCEECEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LFALGIVFHLIGLM
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N p -phosphohistidine; sugar; phosphoenolpyruvate; glucosamine [Periplasm]; phosphoenolpyruvate; beta-D-glucose [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; fructose [Periplasm] [C]

Specific reaction: protein N p -phosphohistidine + sugar = protein histidine + sugar phosphate phosphoenolpyruvate + glucosamine [Periplasm] = D-glucosamine-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm]

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]