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The map label for this gene is nagE [H]

Identifier: 226950247

GI number: 226950247

Start: 3224832

End: 3226274

Strand: Direct

Name: nagE [H]

Synonym: CLM_3202

Alternate gene names: 226950247

Gene position: 3224832-3226274 (Clockwise)

Preceding gene: 226950245

Following gene: 226950248

Centisome position: 77.61

GC content: 32.78

Gene sequence:

>1443_bases
ATGGCGAAAAACAGTAAATTTTTATCAAAAATTCAAAGGCTAGGTAAATCTCTTATGACTCCTATAGCTGTATTGCCAGC
AGCTGCCCTACTTCTTAGATTAGGACAGCCTGATATTTGGAGTTGGGCTGGTTCTTTACCAAGTGGTATCCCTTGGATGT
CTACAGCTGGAGCTGCTATTTTTGATAACTTATCCCTTATTTTTGCTATAGGTATAGCTGTAGGATTAGCAGAGGAAAAT
AATGGCGTTGCTGCGATTTCTGCTACTGTTGGTTTCCTTGTTCTAACAAAGGTTTCTTTAAAATTTGATGATACCATTAA
TATGGGAGTCTTATCCGGTATAATTGTAGGTATATTATCTGCATCACTTTATAATAAGTATAAAAATATTAAACTTCCAG
ATTATCTAGGTTTCTTTGGAGGAAAACGTTTTGTTCCAATTATAACTTCTTTATACTCTTTAATTTTAGGTATTATATCA
GGATTTGTATGGCCTCCAATCCAAAATGTTATAAATAACTTTGGTAATGCAGTTGCAGGAGCTGGTGCTATTGGAGCATT
CTTCTTTGGATTATTTAATAGATTGTTGATTCCAACTGGATTACACCATATTATGAACACAATATTTTGGTTCCAATTTG
GTACATTTAAAAGTCCGTCAGGTACCATTGCTAATGGTGACTTAAAAAGATTCTTCGCTGGGGATCCTACTGCAGGAACT
TATATGACTGGATTCTTTGTAATAATGATGTTTGCTCTTCCTGCAGCCTGTTTTGCTATGATCAAAGCTGCTAGAGAAGA
AAATAAAAAAGCAGTTTCAGGTATGCTTTTAGGTTTAGCATTTACTGCATTCCTTACAGGAGTTACTGAACCAATAGAAT
TTACATTTATGTTTATTTCTCCTGTTCTTTATGCAATACATGCTTTGTTGACTGGTATAGCTCTCGCCCTTACATCAAGC
TTAGGAATAAAATGTGGATTTGGATTTTCTGCAAGTCTTATAGATTATGGTCTTAACTTTGGTATATCAACTAAACCACT
ACTTATAATTCCCATAGGTTTAATATTTGCAGCAATCTATTATTTCCTATTCTTATTCTTTATAAAAAAATTCAATTTAC
CTACACCAGGAAGAGAAGCAGATTCAGAAGTTAATACAAATATTAATGTTGACACTACTAAGAAAAGTGGACACTCTAAA
TTAGAAGATAAAGCTAAAGAAATATTAAAAGCTATAGGCAATTCTGATAATATTGAATCTATAGATGCTTGCGTAACTAG
AATAAGACTTGTTGTTTTTGATGGAAGCAAAATAAATGAAGCCAAACTTAAGGAATTAGGCGCTAATGGAATTATGAAGC
TTGATGATAAAAATTTCCAAATAGTAGTTGGTACTATAGCTGATCCTTTAGTATCTCAAATGAAACTATTAATGAAAAAA
TAA

Upstream 100 bases:

>100_bases
TAATAAATAAATTCTTAAGTTAATTAAAGATGTATTCTACTAAAATATCTATACAAATTAAATAGAAGTATATTATTAAA
TATGTATAGGAGGTATAAAC

Downstream 100 bases:

>100_bases
ATTACTTTATAACTTAAACTATATTTCTTATGTATAACTTAAAAGCCTACAAATATATGAATATTTATATTTGTAGGCTT
TTATCTATAGTTATCTATTA

Product: PTS system N-acetylglucosamine-specific transporter subunit IIBC

Products: NA

Alternate protein names: EIICBA-Nag; EII-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component; N-acetylglucosamine-specific phosphotransferase enzyme IIA component; PTS system N-acetylglucosamine-specific EIIA component [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MAKNSKFLSKIQRLGKSLMTPIAVLPAAALLLRLGQPDIWSWAGSLPSGIPWMSTAGAAIFDNLSLIFAIGIAVGLAEEN
NGVAAISATVGFLVLTKVSLKFDDTINMGVLSGIIVGILSASLYNKYKNIKLPDYLGFFGGKRFVPIITSLYSLILGIIS
GFVWPPIQNVINNFGNAVAGAGAIGAFFFGLFNRLLIPTGLHHIMNTIFWFQFGTFKSPSGTIANGDLKRFFAGDPTAGT
YMTGFFVIMMFALPAACFAMIKAAREENKKAVSGMLLGLAFTAFLTGVTEPIEFTFMFISPVLYAIHALLTGIALALTSS
LGIKCGFGFSASLIDYGLNFGISTKPLLIIPIGLIFAAIYYFLFLFFIKKFNLPTPGREADSEVNTNINVDTTKKSGHSK
LEDKAKEILKAIGNSDNIESIDACVTRIRLVVFDGSKINEAKLKELGANGIMKLDDKNFQIVVGTIADPLVSQMKLLMKK

Sequences:

>Translated_480_residues
MAKNSKFLSKIQRLGKSLMTPIAVLPAAALLLRLGQPDIWSWAGSLPSGIPWMSTAGAAIFDNLSLIFAIGIAVGLAEEN
NGVAAISATVGFLVLTKVSLKFDDTINMGVLSGIIVGILSASLYNKYKNIKLPDYLGFFGGKRFVPIITSLYSLILGIIS
GFVWPPIQNVINNFGNAVAGAGAIGAFFFGLFNRLLIPTGLHHIMNTIFWFQFGTFKSPSGTIANGDLKRFFAGDPTAGT
YMTGFFVIMMFALPAACFAMIKAAREENKKAVSGMLLGLAFTAFLTGVTEPIEFTFMFISPVLYAIHALLTGIALALTSS
LGIKCGFGFSASLIDYGLNFGISTKPLLIIPIGLIFAAIYYFLFLFFIKKFNLPTPGREADSEVNTNINVDTTKKSGHSK
LEDKAKEILKAIGNSDNIESIDACVTRIRLVVFDGSKINEAKLKELGANGIMKLDDKNFQIVVGTIADPLVSQMKLLMKK
>Mature_479_residues
AKNSKFLSKIQRLGKSLMTPIAVLPAAALLLRLGQPDIWSWAGSLPSGIPWMSTAGAAIFDNLSLIFAIGIAVGLAEENN
GVAAISATVGFLVLTKVSLKFDDTINMGVLSGIIVGILSASLYNKYKNIKLPDYLGFFGGKRFVPIITSLYSLILGIISG
FVWPPIQNVINNFGNAVAGAGAIGAFFFGLFNRLLIPTGLHHIMNTIFWFQFGTFKSPSGTIANGDLKRFFAGDPTAGTY
MTGFFVIMMFALPAACFAMIKAAREENKKAVSGMLLGLAFTAFLTGVTEPIEFTFMFISPVLYAIHALLTGIALALTSSL
GIKCGFGFSASLIDYGLNFGISTKPLLIIPIGLIFAAIYYFLFLFFIKKFNLPTPGREADSEVNTNINVDTTKKSGHSKL
EDKAKEILKAIGNSDNIESIDACVTRIRLVVFDGSKINEAKLKELGANGIMKLDDKNFQIVVGTIADPLVSQMKLLMKK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=472, Percent_Identity=48.5169491525424, Blast_Score=428, Evalue=1e-121,
Organism=Escherichia coli, GI1787343, Length=495, Percent_Identity=39.1919191919192, Blast_Score=350, Evalue=1e-97,
Organism=Escherichia coli, GI1787908, Length=516, Percent_Identity=34.8837209302326, Blast_Score=270, Evalue=1e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010974 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 51702; Mature: 51571

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNSKFLSKIQRLGKSLMTPIAVLPAAALLLRLGQPDIWSWAGSLPSGIPWMSTAGAAI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHCCCHHH
FDNLSLIFAIGIAVGLAEENNGVAAISATVGFLVLTKVSLKFDDTINMGVLSGIIVGILS
HHHHHHHHHHHHHHHEEECCCCEEEHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHH
ASLYNKYKNIKLPDYLGFFGGKRFVPIITSLYSLILGIISGFVWPPIQNVINNFGNAVAG
HHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHC
AGAIGAFFFGLFNRLLIPTGLHHIMNTIFWFQFGTFKSPSGTIANGDLKRFFAGDPTAGT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCH
YMTGFFVIMMFALPAACFAMIKAAREENKKAVSGMLLGLAFTAFLTGVTEPIEFTFMFIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
PVLYAIHALLTGIALALTSSLGIKCGFGFSASLIDYGLNFGISTKPLLIIPIGLIFAAIY
HHHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHCCCCCCCCCCHHEHHHHHHHHHHH
YFLFLFFIKKFNLPTPGREADSEVNTNINVDTTKKSGHSKLEDKAKEILKAIGNSDNIES
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCHHH
IDACVTRIRLVVFDGSKINEAKLKELGANGIMKLDDKNFQIVVGTIADPLVSQMKLLMKK
HHHHHHHHEEEEECCCCCCHHHHHHHCCCCCEEECCCCCEEEEECHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKNSKFLSKIQRLGKSLMTPIAVLPAAALLLRLGQPDIWSWAGSLPSGIPWMSTAGAAI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHCCCHHH
FDNLSLIFAIGIAVGLAEENNGVAAISATVGFLVLTKVSLKFDDTINMGVLSGIIVGILS
HHHHHHHHHHHHHHHEEECCCCEEEHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHH
ASLYNKYKNIKLPDYLGFFGGKRFVPIITSLYSLILGIISGFVWPPIQNVINNFGNAVAG
HHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHC
AGAIGAFFFGLFNRLLIPTGLHHIMNTIFWFQFGTFKSPSGTIANGDLKRFFAGDPTAGT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCH
YMTGFFVIMMFALPAACFAMIKAAREENKKAVSGMLLGLAFTAFLTGVTEPIEFTFMFIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
PVLYAIHALLTGIALALTSSLGIKCGFGFSASLIDYGLNFGISTKPLLIIPIGLIFAAIY
HHHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHCCCCCCCCCCHHEHHHHHHHHHHH
YFLFLFFIKKFNLPTPGREADSEVNTNINVDTTKKSGHSKLEDKAKEILKAIGNSDNIES
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCHHH
IDACVTRIRLVVFDGSKINEAKLKELGANGIMKLDDKNFQIVVGTIADPLVSQMKLLMKK
HHHHHHHHEEEEECCCCCCHHHHHHHCCCCCEEECCCCCEEEEECHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3284790; 3056518; 8905232; 9278503 [H]