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The map label for this gene is adhE [H]

Identifier: 226949402

GI number: 226949402

Start: 2401468

End: 2402961

Strand: Reverse

Name: adhE [H]

Synonym: CLM_2327

Alternate gene names: 226949402

Gene position: 2402961-2401468 (Counterclockwise)

Preceding gene: 226949403

Following gene: 226949401

Centisome position: 57.83

GC content: 33.4

Gene sequence:

>1494_bases
ATGAATATTATAGATAATGATTTACTCTCCATGCAGGAAGCTAGAATTCTTGTTGAAAATGCCCGAGAGGCACAAAAAAA
ATTAGCTACTTTCCCACAAGAAAAACTTGATGAGATAGTTGAGCGTATGGCTGAAGAGGTTGAAAAACATTTAAAAGAGC
TTGCAAAAATATCTAATGAAGAAACAGAGTATGGAAAGTGGCAAGATAAATATGTTAAAAATCACTTTGTATGTGAGTAT
TTAAAAAACAAACTCAAAGGTATGAGGTGTGTAGGTATTATCAATGAAGATAAAATTAATAAAACCATGGATGTTGGTGT
ACCAATGGGGGTTGTTATAGCCTTCCCACCAGCAACAAGTCCAGTATCTACCACTATATATAAAGCATTAATTGCAATAA
AGTCAGGAAATGCGATTATATTTTCACCTCATAAAAGAGCTAAAAATACAATTTCTAAAACAGTGGATATTTTAATTAGA
GCTGCAGAAGGTGTAGGACTTCCAGAAGGTGCTCTTGCATATCTTCACACAGTAACTGAAAGTGGTTCATTAGAGCTTAT
GAAGCATAAAGATACATCTTTAATAATGAACACTGGAGTTATAGAAATGCTTGACGAAGCCTATAAATCTGGTAAGCCAG
TAATATATGGAGGCAATGGAAACGGACCTGCCTTTATAGAACGTACAGCTGATATAGAAAAAGCGGTTAAAGATATTATT
ACAAGCAAGAATTTTGATTATGGAATTGTATCTGCCGCAGAACAATCAATTGTTGTAGATAGCTTAATTGCATCTGAAGT
AAAGCAAGAACTTATTAAAAATGGTGGATATTTTATGACGGAAGAAGAAGCTTTAAAACTAGGAAAGATTTTGTTTAACC
AAAATGGAAGCCCAGATTTAGAGATGATTGGTAAGTCTCCACAATTTTTAGCTAAAAAGGCTGGCTTTGAAGTTTCAGAA
GGTGTAAAAGTGCTTATTTCCAATGAGAAGTTTGTTTCTAAAAATAATCCTTATTCAAGGGAAAAGCTTTCTCCGGTTTT
AGCCTTTTATATAGAGGATGACTGGATGCACGCTTGTGAAAAATGTATAGAATTATTACTTACTGAAAGATATGGACATA
CACTTGTGATACATTCAAAGGATGAAGAAGTTATTCGTCAATTTGCACTTAAAAAACCCGTTGGGAGAGTACTTGTAAAT
ACTGCAGGAACTTTTGGAAGCATGGGAGCTACTACAAATTTGTTTCCTTCAATGACATTAGGTAGTGGTTCTGCAGGTCA
AGGAATGACTTCGGATAATGTTTCACCTATGAATCTATTATATATACGTAAAGTTGGATATGGAGTTCGCATGGTGGATG
AAATTGTTAAAACAGTAAATGATAAAGAGGATTCTATCCCTGCAGTTTTAAATGGGAAGTTAAATGGAAATGAAATAGAT
AACTATAAGTTATTAGAGAGTATTTTAAAAAAAGTTCTACAGGACTTAAAATAA

Upstream 100 bases:

>100_bases
CCACACGAAGATCTTGAAAAAATAATAAAACGTTATGCCATTACAGATCTTATAACAGAATAGAAAAATGAATTTTTTCT
AAAGAAAAGGAGAGAGAAAG

Downstream 100 bases:

>100_bases
AAAATCATATTTAATATATGAAATACATTAAAGAATGTGAGGGAATAAATTGGATATACGTGAATTTTCAAATAAATTTA
TGGAAGCTACTAAAAATATG

Product: aldehyde dehydrogenase family protein

Products: NA

Alternate protein names: Alcohol dehydrogenase; ADH; Acetaldehyde dehydrogenase [acetylating]; ACDH; Pyruvate-formate-lyase deactivase; PFL deactivase [H]

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MNIIDNDLLSMQEARILVENAREAQKKLATFPQEKLDEIVERMAEEVEKHLKELAKISNEETEYGKWQDKYVKNHFVCEY
LKNKLKGMRCVGIINEDKINKTMDVGVPMGVVIAFPPATSPVSTTIYKALIAIKSGNAIIFSPHKRAKNTISKTVDILIR
AAEGVGLPEGALAYLHTVTESGSLELMKHKDTSLIMNTGVIEMLDEAYKSGKPVIYGGNGNGPAFIERTADIEKAVKDII
TSKNFDYGIVSAAEQSIVVDSLIASEVKQELIKNGGYFMTEEEALKLGKILFNQNGSPDLEMIGKSPQFLAKKAGFEVSE
GVKVLISNEKFVSKNNPYSREKLSPVLAFYIEDDWMHACEKCIELLLTERYGHTLVIHSKDEEVIRQFALKKPVGRVLVN
TAGTFGSMGATTNLFPSMTLGSGSAGQGMTSDNVSPMNLLYIRKVGYGVRMVDEIVKTVNDKEDSIPAVLNGKLNGNEID
NYKLLESILKKVLQDLK

Sequences:

>Translated_497_residues
MNIIDNDLLSMQEARILVENAREAQKKLATFPQEKLDEIVERMAEEVEKHLKELAKISNEETEYGKWQDKYVKNHFVCEY
LKNKLKGMRCVGIINEDKINKTMDVGVPMGVVIAFPPATSPVSTTIYKALIAIKSGNAIIFSPHKRAKNTISKTVDILIR
AAEGVGLPEGALAYLHTVTESGSLELMKHKDTSLIMNTGVIEMLDEAYKSGKPVIYGGNGNGPAFIERTADIEKAVKDII
TSKNFDYGIVSAAEQSIVVDSLIASEVKQELIKNGGYFMTEEEALKLGKILFNQNGSPDLEMIGKSPQFLAKKAGFEVSE
GVKVLISNEKFVSKNNPYSREKLSPVLAFYIEDDWMHACEKCIELLLTERYGHTLVIHSKDEEVIRQFALKKPVGRVLVN
TAGTFGSMGATTNLFPSMTLGSGSAGQGMTSDNVSPMNLLYIRKVGYGVRMVDEIVKTVNDKEDSIPAVLNGKLNGNEID
NYKLLESILKKVLQDLK
>Mature_497_residues
MNIIDNDLLSMQEARILVENAREAQKKLATFPQEKLDEIVERMAEEVEKHLKELAKISNEETEYGKWQDKYVKNHFVCEY
LKNKLKGMRCVGIINEDKINKTMDVGVPMGVVIAFPPATSPVSTTIYKALIAIKSGNAIIFSPHKRAKNTISKTVDILIR
AAEGVGLPEGALAYLHTVTESGSLELMKHKDTSLIMNTGVIEMLDEAYKSGKPVIYGGNGNGPAFIERTADIEKAVKDII
TSKNFDYGIVSAAEQSIVVDSLIASEVKQELIKNGGYFMTEEEALKLGKILFNQNGSPDLEMIGKSPQFLAKKAGFEVSE
GVKVLISNEKFVSKNNPYSREKLSPVLAFYIEDDWMHACEKCIELLLTERYGHTLVIHSKDEEVIRQFALKKPVGRVLVN
TAGTFGSMGATTNLFPSMTLGSGSAGQGMTSDNVSPMNLLYIRKVGYGVRMVDEIVKTVNDKEDSIPAVLNGKLNGNEID
NYKLLESILKKVLQDLK

Specific function: This enzyme has three activities:ADH, ACDH, and PFL- deactivase. In aerobic conditions it acts as a hydrogen peroxide scavenger. The PFL deactivase activity catalyzes the quenching of the pyruvate-formate-lyase catalyst in an iron, NAD, and CoA dependent

COG id: COG1012

COG function: function code C; NAD-dependent aldehyde dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the iron- containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1787493, Length=469, Percent_Identity=35.8208955223881, Blast_Score=280, Evalue=2e-76,
Organism=Escherichia coli, GI1788797, Length=356, Percent_Identity=26.9662921348315, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI87081926, Length=317, Percent_Identity=22.397476340694, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR012079 [H]

Pfam domain/function: PF00171 Aldedh; PF00465 Fe-ADH [H]

EC number: =1.1.1.1; =1.2.1.10 [H]

Molecular weight: Translated: 54949; Mature: 54949

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIIDNDLLSMQEARILVENAREAQKKLATFPQEKLDEIVERMAEEVEKHLKELAKISNE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ETEYGKWQDKYVKNHFVCEYLKNKLKGMRCVGIINEDKINKTMDVGVPMGVVIAFPPATS
CCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHCCCCCCEEEEEECCCCC
PVSTTIYKALIAIKSGNAIIFSPHKRAKNTISKTVDILIRAAEGVGLPEGALAYLHTVTE
CHHHHHHHHHHHEECCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
SGSLELMKHKDTSLIMNTGVIEMLDEAYKSGKPVIYGGNGNGPAFIERTADIEKAVKDII
CCCEEEEECCCCEEEECCCHHHHHHHHHHCCCCEEECCCCCCCEEEEHHHHHHHHHHHHH
TSKNFDYGIVSAAEQSIVVDSLIASEVKQELIKNGGYFMTEEEALKLGKILFNQNGSPDL
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCCCH
EMIGKSPQFLAKKAGFEVSEGVKVLISNEKFVSKNNPYSREKLSPVLAFYIEDDWMHACE
HCCCCCHHHHHHHCCCCCCCCCEEEEECCEEECCCCCCCHHHHCCEEEEEECCHHHHHHH
KCIELLLTERYGHTLVIHSKDEEVIRQFALKKPVGRVLVNTAGTFGSMGATTNLFPSMTL
HHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCC
GSGSAGQGMTSDNVSPMNLLYIRKVGYGVRMVDEIVKTVNDKEDSIPAVLNGKLNGNEID
CCCCCCCCCCCCCCCCHHEEEEHHHCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCC
NYKLLESILKKVLQDLK
CHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNIIDNDLLSMQEARILVENAREAQKKLATFPQEKLDEIVERMAEEVEKHLKELAKISNE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ETEYGKWQDKYVKNHFVCEYLKNKLKGMRCVGIINEDKINKTMDVGVPMGVVIAFPPATS
CCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHCCCCCCEEEEEECCCCC
PVSTTIYKALIAIKSGNAIIFSPHKRAKNTISKTVDILIRAAEGVGLPEGALAYLHTVTE
CHHHHHHHHHHHEECCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
SGSLELMKHKDTSLIMNTGVIEMLDEAYKSGKPVIYGGNGNGPAFIERTADIEKAVKDII
CCCEEEEECCCCEEEECCCHHHHHHHHHHCCCCEEECCCCCCCEEEEHHHHHHHHHHHHH
TSKNFDYGIVSAAEQSIVVDSLIASEVKQELIKNGGYFMTEEEALKLGKILFNQNGSPDL
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCCCH
EMIGKSPQFLAKKAGFEVSEGVKVLISNEKFVSKNNPYSREKLSPVLAFYIEDDWMHACE
HCCCCCHHHHHHHCCCCCCCCCEEEEECCEEECCCCCCCHHHHCCEEEEEECCHHHHHHH
KCIELLLTERYGHTLVIHSKDEEVIRQFALKKPVGRVLVNTAGTFGSMGATTNLFPSMTL
HHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCC
GSGSAGQGMTSDNVSPMNLLYIRKVGYGVRMVDEIVKTVNDKEDSIPAVLNGKLNGNEID
CCCCCCCCCCCCCCCCHHEEEEHHHCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCC
NYKLLESILKKVLQDLK
CHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]