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The map label for this gene is ygeV [H]

Identifier: 226949307

GI number: 226949307

Start: 2293448

End: 2295223

Strand: Reverse

Name: ygeV [H]

Synonym: CLM_2227

Alternate gene names: 226949307

Gene position: 2295223-2293448 (Counterclockwise)

Preceding gene: 226949310

Following gene: 226949306

Centisome position: 55.24

GC content: 28.21

Gene sequence:

>1776_bases
GTGTCTTTAAATAGTATAAAGGACTCTGTTCAAGAAGTAGCAGAGGCTATTGCTGAAGTTCTTAAGGTGGATGTTACTAT
AATTGATAAAAATTTTAATAGAATTGCCGCCACCGGTAAGTATAAAAAATTTATAGGAAATAAAATACCAGGAAAATGTT
TGTTTGAATTAGTTATAAAAGAGAAAAAAACCAATCATATAAAAAGATATTTAAAGGATAATAAAAAGAAGATAAATCCA
TCTGTATGTGAAAGCTGTGAGGCAAAGGAGAGATGTACAGAGTATGCAACCATAGGTTACCCTATAATTATGGAAGATGA
GGTAATAGGGGTAATTGGTATAAATATTTTTAATGAAAGACAACTTAATATAATATCTGAAGAATTTGATTCTATGATAA
TATTTTTAAATAGATTAAGCACATTACTTGTAGGAAATATTGTGTATTATGATACTATAAAAAAATTACAAATTCAGACT
GAGGAAACTAACCATATAATAGATAGTTTAAGCCACGGTATAATGTGTGTAGATAACAATGGAATTTTAAAATATATTAA
TCATAAGGGTAAAATTCTATTAGATATTAAAGAGAAGTCATTAGTAAACACTAATATTAAAGATACAATATCAAACTTTA
ATATTAAACTTTTAAGTAAAGAATATAAGGGTAAAAGAATAAGTATCCATGGCAAAAATGAAAGTTTTTTAATTAAAAGT
AGTCCCATTATATTCAAGGGTGAAAGGGTAAGTAATATTATAGAATTTAATAAAAAAATTGATGAAGTTCAAGCTGCCTA
TAAGCTTTTCGCTAGTAGTAAAATTATAAAATTTGAAGATATAATGGGTAGTAGTAGCAATATAAAAAATGTAAAACTCA
TTGCTAAAAATATTGCTAAAACTGATTCCACAGTGTTATTAAGGGGAGAGAGTGGTACAGGAAAGGAGCTCTTTGCAAGG
TCTATACATTTTGAGAGTAGAAGATATAATGCTCCTTTTATAGCTGTTAATTGTGCTTCTATTCCAGATAATTTATTAGA
AAGTGAATTTTTTGGATATGAAGGAGGAGCCTTTTCAGGAGCAAAAAGGGAAGGACAAATGGGGAAATTTGAACTTGCCA
ATGGAGGGACTATATTTTTAGATGAAATAGGTGATTTGCCATTACATCTTCAACCTAAAATATTAAGAGTACTTCAAGAA
CAAAGTTTTACTAGAATTGGTGGCAAAGAAGAAATATGCGTAGATGTTAGAATAATAGCAGCTACCAATAGAGATCTTGA
AAGTATGGTTAAGCATGGACAATTTAGACAGGATTTATATTATAGATTAAATGTAATACCTATATATTTGCCTAGTCTTA
AAGATAGAGGGGAAGATATAGTATTACTTAGTGAATATCTTATGGATAGATTTTGCGAAAGATCTAATCTACAAATTAAA
CAACTTTCAAAGGAAGTAAAGGATAGTTTTAAGAAATATCCTTGGCCTGGCAACATAAGAGAACTGGAAAATGTAATAGA
ATATATAGTAAATACCACAAAGGAAAATGTAATATATAATGAGCATTTACCAAAATCCTTTAAGATATCTAAAGAGCATA
GGAAAAATAATAAAAGTTTGCAGGATAGACTTTGGGAATATGAAAGAAATTTATTAATATCAATGATGGATGAGTACGGT
GAAGATGGGAAAGGGAAAGAAAGAATTGCCAGAGAACTAGATATTAATTTATCTACACTCTACAGAAAATTAAATAAATA
TAATTTGCAAAAATGA

Upstream 100 bases:

>100_bases
CAAAAGGATATAGAAAACAAAGAGAGGGGGAGTTACGTTAAAACAAAAGCATTATATCTGTATGCAAAATAATTTTAAAA
GTTTTACGTAACGAATAAAA

Downstream 100 bases:

>100_bases
GAATGTTTTTGCAAAGATGCAAAAATCCTTTAATATGAATCTATAATGCATTAATTTTTATAATTAATAGGAGGATTATT
TGCAAAAATGCGAAAATATA

Product: putative sigma-54 dependent transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 591; Mature: 590

Protein sequence:

>591_residues
MSLNSIKDSVQEVAEAIAEVLKVDVTIIDKNFNRIAATGKYKKFIGNKIPGKCLFELVIKEKKTNHIKRYLKDNKKKINP
SVCESCEAKERCTEYATIGYPIIMEDEVIGVIGINIFNERQLNIISEEFDSMIIFLNRLSTLLVGNIVYYDTIKKLQIQT
EETNHIIDSLSHGIMCVDNNGILKYINHKGKILLDIKEKSLVNTNIKDTISNFNIKLLSKEYKGKRISIHGKNESFLIKS
SPIIFKGERVSNIIEFNKKIDEVQAAYKLFASSKIIKFEDIMGSSSNIKNVKLIAKNIAKTDSTVLLRGESGTGKELFAR
SIHFESRRYNAPFIAVNCASIPDNLLESEFFGYEGGAFSGAKREGQMGKFELANGGTIFLDEIGDLPLHLQPKILRVLQE
QSFTRIGGKEEICVDVRIIAATNRDLESMVKHGQFRQDLYYRLNVIPIYLPSLKDRGEDIVLLSEYLMDRFCERSNLQIK
QLSKEVKDSFKKYPWPGNIRELENVIEYIVNTTKENVIYNEHLPKSFKISKEHRKNNKSLQDRLWEYERNLLISMMDEYG
EDGKGKERIARELDINLSTLYRKLNKYNLQK

Sequences:

>Translated_591_residues
MSLNSIKDSVQEVAEAIAEVLKVDVTIIDKNFNRIAATGKYKKFIGNKIPGKCLFELVIKEKKTNHIKRYLKDNKKKINP
SVCESCEAKERCTEYATIGYPIIMEDEVIGVIGINIFNERQLNIISEEFDSMIIFLNRLSTLLVGNIVYYDTIKKLQIQT
EETNHIIDSLSHGIMCVDNNGILKYINHKGKILLDIKEKSLVNTNIKDTISNFNIKLLSKEYKGKRISIHGKNESFLIKS
SPIIFKGERVSNIIEFNKKIDEVQAAYKLFASSKIIKFEDIMGSSSNIKNVKLIAKNIAKTDSTVLLRGESGTGKELFAR
SIHFESRRYNAPFIAVNCASIPDNLLESEFFGYEGGAFSGAKREGQMGKFELANGGTIFLDEIGDLPLHLQPKILRVLQE
QSFTRIGGKEEICVDVRIIAATNRDLESMVKHGQFRQDLYYRLNVIPIYLPSLKDRGEDIVLLSEYLMDRFCERSNLQIK
QLSKEVKDSFKKYPWPGNIRELENVIEYIVNTTKENVIYNEHLPKSFKISKEHRKNNKSLQDRLWEYERNLLISMMDEYG
EDGKGKERIARELDINLSTLYRKLNKYNLQK
>Mature_590_residues
SLNSIKDSVQEVAEAIAEVLKVDVTIIDKNFNRIAATGKYKKFIGNKIPGKCLFELVIKEKKTNHIKRYLKDNKKKINPS
VCESCEAKERCTEYATIGYPIIMEDEVIGVIGINIFNERQLNIISEEFDSMIIFLNRLSTLLVGNIVYYDTIKKLQIQTE
ETNHIIDSLSHGIMCVDNNGILKYINHKGKILLDIKEKSLVNTNIKDTISNFNIKLLSKEYKGKRISIHGKNESFLIKSS
PIIFKGERVSNIIEFNKKIDEVQAAYKLFASSKIIKFEDIMGSSSNIKNVKLIAKNIAKTDSTVLLRGESGTGKELFARS
IHFESRRYNAPFIAVNCASIPDNLLESEFFGYEGGAFSGAKREGQMGKFELANGGTIFLDEIGDLPLHLQPKILRVLQEQ
SFTRIGGKEEICVDVRIIAATNRDLESMVKHGQFRQDLYYRLNVIPIYLPSLKDRGEDIVLLSEYLMDRFCERSNLQIKQ
LSKEVKDSFKKYPWPGNIRELENVIEYIVNTTKENVIYNEHLPKSFKISKEHRKNNKSLQDRLWEYERNLLISMMDEYGE
DGKGKERIARELDINLSTLYRKLNKYNLQK

Specific function: Unknown

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789233, Length=607, Percent_Identity=33.1136738056013, Blast_Score=340, Evalue=1e-94,
Organism=Escherichia coli, GI1788550, Length=326, Percent_Identity=43.2515337423313, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI87082117, Length=244, Percent_Identity=52.0491803278688, Blast_Score=255, Evalue=6e-69,
Organism=Escherichia coli, GI1789087, Length=315, Percent_Identity=42.8571428571429, Blast_Score=248, Evalue=7e-67,
Organism=Escherichia coli, GI1790437, Length=289, Percent_Identity=43.598615916955, Blast_Score=247, Evalue=2e-66,
Organism=Escherichia coli, GI1790299, Length=245, Percent_Identity=44.8979591836735, Blast_Score=230, Evalue=2e-61,
Organism=Escherichia coli, GI1788905, Length=228, Percent_Identity=48.2456140350877, Blast_Score=227, Evalue=2e-60,
Organism=Escherichia coli, GI1786524, Length=243, Percent_Identity=43.2098765432099, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1787583, Length=434, Percent_Identity=29.2626728110599, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI87082152, Length=235, Percent_Identity=44.2553191489362, Blast_Score=205, Evalue=6e-54,
Organism=Escherichia coli, GI87081872, Length=233, Percent_Identity=42.0600858369099, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI87081858, Length=459, Percent_Identity=25.9259259259259, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1789828, Length=226, Percent_Identity=34.070796460177, Blast_Score=133, Evalue=3e-32,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR000014
- InterPro:   IPR002078 [H]

Pfam domain/function: PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 67877; Mature: 67746

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLNSIKDSVQEVAEAIAEVLKVDVTIIDKNFNRIAATGKYKKFIGNKIPGKCLFELVIK
CCCHHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEECCCHHHHHHCCCCCHHHHHHHHHH
EKKTNHIKRYLKDNKKKINPSVCESCEAKERCTEYATIGYPIIMEDEVIGVIGINIFNER
HHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHCCCCEEECCCEEEEEEEEEECCC
QLNIISEEFDSMIIFLNRLSTLLVGNIVYYDTIKKLQIQTEETNHIIDSLSHGIMCVDNN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHEEEECCHHHHHHHHHCCCEEEEECC
GILKYINHKGKILLDIKEKSLVNTNIKDTISNFNIKLLSKEYKGKRISIHGKNESFLIKS
CEEEEECCCCCEEEEECCCCCCCCCHHHHHCCCCEEEEECCCCCCEEEEECCCCEEEEEC
SPIIFKGERVSNIIEFNKKIDEVQAAYKLFASSKIIKFEDIMGSSSNIKNVKLIAKNIAK
CCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHCCCCCCCCHHHHHHHHHCC
TDSTVLLRGESGTGKELFARSIHFESRRYNAPFIAVNCASIPDNLLESEFFGYEGGAFSG
CCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCC
AKREGQMGKFELANGGTIFLDEIGDLPLHLQPKILRVLQEQSFTRIGGKEEICVDVRIIA
CCCCCCCCEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEE
ATNRDLESMVKHGQFRQDLYYRLNVIPIYLPSLKDRGEDIVLLSEYLMDRFCERSNLQIK
ECCCCHHHHHHCCCHHHHHHEEEEEEEEEECCCCCCCCCEEEHHHHHHHHHHCCCCCHHH
QLSKEVKDSFKKYPWPGNIRELENVIEYIVNTTKENVIYNEHLPKSFKISKEHRKNNKSL
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHCCCCCHH
QDRLWEYERNLLISMMDEYGEDGKGKERIARELDINLSTLYRKLNKYNLQK
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SLNSIKDSVQEVAEAIAEVLKVDVTIIDKNFNRIAATGKYKKFIGNKIPGKCLFELVIK
CCHHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEECCCHHHHHHCCCCCHHHHHHHHHH
EKKTNHIKRYLKDNKKKINPSVCESCEAKERCTEYATIGYPIIMEDEVIGVIGINIFNER
HHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHCCCCEEECCCEEEEEEEEEECCC
QLNIISEEFDSMIIFLNRLSTLLVGNIVYYDTIKKLQIQTEETNHIIDSLSHGIMCVDNN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHEEEECCHHHHHHHHHCCCEEEEECC
GILKYINHKGKILLDIKEKSLVNTNIKDTISNFNIKLLSKEYKGKRISIHGKNESFLIKS
CEEEEECCCCCEEEEECCCCCCCCCHHHHHCCCCEEEEECCCCCCEEEEECCCCEEEEEC
SPIIFKGERVSNIIEFNKKIDEVQAAYKLFASSKIIKFEDIMGSSSNIKNVKLIAKNIAK
CCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHCCCCCCCCHHHHHHHHHCC
TDSTVLLRGESGTGKELFARSIHFESRRYNAPFIAVNCASIPDNLLESEFFGYEGGAFSG
CCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCC
AKREGQMGKFELANGGTIFLDEIGDLPLHLQPKILRVLQEQSFTRIGGKEEICVDVRIIA
CCCCCCCCEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEE
ATNRDLESMVKHGQFRQDLYYRLNVIPIYLPSLKDRGEDIVLLSEYLMDRFCERSNLQIK
ECCCCHHHHHHCCCHHHHHHEEEEEEEEEECCCCCCCCCEEEHHHHHHHHHHCCCCCHHH
QLSKEVKDSFKKYPWPGNIRELENVIEYIVNTTKENVIYNEHLPKSFKISKEHRKNNKSL
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHCCCCCHH
QDRLWEYERNLLISMMDEYGEDGKGKERIARELDINLSTLYRKLNKYNLQK
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]