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The map label for this gene is fruA [H]

Identifier: 226949289

GI number: 226949289

Start: 2275259

End: 2276656

Strand: Reverse

Name: fruA [H]

Synonym: CLM_2208

Alternate gene names: 226949289

Gene position: 2276656-2275259 (Counterclockwise)

Preceding gene: 226949290

Following gene: 226949288

Centisome position: 54.79

GC content: 34.69

Gene sequence:

>1398_bases
ATGAAAAAGATTATTGCTGTTACGGGATGTCCTACAGGAATAGCTCATACATTTATGGCAGAAGAAGCATTAAAGAAAGC
AGCTAAAGAATTAGGAATAGACATAAAAGTAGAAACTAATGGTGCTGTTGGAGTAGAAAATGAATTAACAGAAAAGGATA
TAGAAGAAGCAATTGGAGTAATAGTCGCATCAGATAAAGATGTTAGAGCTGATAGATTTACAGGAAGACCAACTATAGAA
ACTTCAGTAGCAGATGGAATTCGTAAACCTAAAGAGTTAATTGAAGCTATATTAGATGGAAAGGCTCCTATAAAAGGAGG
AGCAGCAGCTAAAAAAACTATAAAGAAAAGCTTAGAAGAAGTTCCGGGTAAAAAATCTGTAACTAGAGATATATATAAAC
ACTTAATGAATGGTGTATCTCATATGTTACCTTTCGTTGTAGCAGGTGGTGTACTTATAGCTATATCTTTCCTATGGGGA
ATATATTCTTTTGAGCCAAAATCAGCACAATATAATCCTATAGCAGCTATGATTAAGGGAATAGGTGGAGAAGCATTTGC
AATAATAGTTCCAATATTTACAGCTTATATTGCAGATTCTATTTCTGGAAGACCAGGTATGGTAGCGGGATTTGTTGGTG
GACTTATAGCAAATACTACAGGATCAGGATTTTTAGGAGGTATCATTGCAGGTTTCTTCGCTGGATATTTGATGCTTTAT
TCTAAAAAACTACTTTCAGGATTACCAAAACAATTTGAAGGTTTAAAATCAATATTTATATTACCAATAATCGGAATATT
AATAACAGGCGTATTAATGACACTATTAGGTGAACCAGTGGCAGCTTTAAATAAGGGTGTTATGGCATGGCTTGCATCGC
TTGAACAAGCAAATCCAATTTTATTAGGAATAATTATAGGTTGTATGTCTGCCTTTGACTTTGGTGGACCAGTAAATAAA
GCTGCTTATTTAACAGGTACAATGCTACTTGCACAAGGAAATTATTACTTCATGGCTGGTGTTTCAGCAGCTTGTATAAC
ACCGCCACTTATAATCGCCTTAGCAACAACATTCTTCAAAAATAGATTTGACGAAGAAGAAAGAGCAGCAGGTTTAGTTA
ATTACATCTTAGGCTGCACTCATATAACAGAGGGAGCAATTCCTTTTGCAGCTAAAGATCCATTAAGGGTTATTCCAATA
TTAATGGTTGGTTCTTCTGTATCTTCTGTATTAACTTATTTAATGAAGATTCAAGTACCAGCTCCTCATGGTGGATTTTT
AATACTGCCATTAGTTAGTAAACCATTACTATGGGTAGCTGCGATATTAGTAGGGTCAACAGTAGGCGCAATATTATATG
GAATATATAGAGGACATACACAAAAGAAAGCTCAATAA

Upstream 100 bases:

>100_bases
ATCAGCCACTGGTGCAAATGTTGCTGAATCTAGTGCATTAGGAACTTTTGGTAAAGTATCAGAGTATATAAAAAAAATAC
ATGTTAAGGAGGTAGTGTAA

Downstream 100 bases:

>100_bases
GAAAGTTAGGATTTATCTGAGGAGATAGCAGATCTTATGCCCAACTTTAGAAAAGCAGATATAAGATAATCTACGACTTT
GTTATATTGGCTTTTGTAGG

Product: PTS system fructose family transporter subunit IIBC

Products: NA

Alternate protein names: EIIABC-Fru; Fructose-specific phosphotransferase enzyme IIA component; EII-Fru; PTS system fructose-specific EIIA component; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MKKIIAVTGCPTGIAHTFMAEEALKKAAKELGIDIKVETNGAVGVENELTEKDIEEAIGVIVASDKDVRADRFTGRPTIE
TSVADGIRKPKELIEAILDGKAPIKGGAAAKKTIKKSLEEVPGKKSVTRDIYKHLMNGVSHMLPFVVAGGVLIAISFLWG
IYSFEPKSAQYNPIAAMIKGIGGEAFAIIVPIFTAYIADSISGRPGMVAGFVGGLIANTTGSGFLGGIIAGFFAGYLMLY
SKKLLSGLPKQFEGLKSIFILPIIGILITGVLMTLLGEPVAALNKGVMAWLASLEQANPILLGIIIGCMSAFDFGGPVNK
AAYLTGTMLLAQGNYYFMAGVSAACITPPLIIALATTFFKNRFDEEERAAGLVNYILGCTHITEGAIPFAAKDPLRVIPI
LMVGSSVSSVLTYLMKIQVPAPHGGFLILPLVSKPLLWVAAILVGSTVGAILYGIYRGHTQKKAQ

Sequences:

>Translated_465_residues
MKKIIAVTGCPTGIAHTFMAEEALKKAAKELGIDIKVETNGAVGVENELTEKDIEEAIGVIVASDKDVRADRFTGRPTIE
TSVADGIRKPKELIEAILDGKAPIKGGAAAKKTIKKSLEEVPGKKSVTRDIYKHLMNGVSHMLPFVVAGGVLIAISFLWG
IYSFEPKSAQYNPIAAMIKGIGGEAFAIIVPIFTAYIADSISGRPGMVAGFVGGLIANTTGSGFLGGIIAGFFAGYLMLY
SKKLLSGLPKQFEGLKSIFILPIIGILITGVLMTLLGEPVAALNKGVMAWLASLEQANPILLGIIIGCMSAFDFGGPVNK
AAYLTGTMLLAQGNYYFMAGVSAACITPPLIIALATTFFKNRFDEEERAAGLVNYILGCTHITEGAIPFAAKDPLRVIPI
LMVGSSVSSVLTYLMKIQVPAPHGGFLILPLVSKPLLWVAAILVGSTVGAILYGIYRGHTQKKAQ
>Mature_465_residues
MKKIIAVTGCPTGIAHTFMAEEALKKAAKELGIDIKVETNGAVGVENELTEKDIEEAIGVIVASDKDVRADRFTGRPTIE
TSVADGIRKPKELIEAILDGKAPIKGGAAAKKTIKKSLEEVPGKKSVTRDIYKHLMNGVSHMLPFVVAGGVLIAISFLWG
IYSFEPKSAQYNPIAAMIKGIGGEAFAIIVPIFTAYIADSISGRPGMVAGFVGGLIANTTGSGFLGGIIAGFFAGYLMLY
SKKLLSGLPKQFEGLKSIFILPIIGILITGVLMTLLGEPVAALNKGVMAWLASLEQANPILLGIIIGCMSAFDFGGPVNK
AAYLTGTMLLAQGNYYFMAGVSAACITPPLIIALATTFFKNRFDEEERAAGLVNYILGCTHITEGAIPFAAKDPLRVIPI
LMVGSSVSSVLTYLMKIQVPAPHGGFLILPLVSKPLLWVAAILVGSTVGAILYGIYRGHTQKKAQ

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788492, Length=462, Percent_Identity=42.2077922077922, Blast_Score=341, Evalue=6e-95,
Organism=Escherichia coli, GI1786951, Length=472, Percent_Identity=35.8050847457627, Blast_Score=262, Evalue=3e-71,
Organism=Escherichia coli, GI1790386, Length=312, Percent_Identity=40.7051282051282, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI87082348, Length=433, Percent_Identity=31.1778290993072, Blast_Score=197, Evalue=9e-52,
Organism=Escherichia coli, GI1788729, Length=404, Percent_Identity=26.7326732673267, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1790387, Length=98, Percent_Identity=43.8775510204082, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788730, Length=99, Percent_Identity=42.4242424242424, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1790390, Length=98, Percent_Identity=33.6734693877551, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR004715
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF00359 PTS_EIIA_2; PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 49070; Mature: 49070

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIIAVTGCPTGIAHTFMAEEALKKAAKELGIDIKVETNGAVGVENELTEKDIEEAIGV
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHCE
IVASDKDVRADRFTGRPTIETSVADGIRKPKELIEAILDGKAPIKGGAAAKKTIKKSLEE
EEECCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
VPGKKSVTRDIYKHLMNGVSHMLPFVVAGGVLIAISFLWGIYSFEPKSAQYNPIAAMIKG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHC
IGGEAFAIIVPIFTAYIADSISGRPGMVAGFVGGLIANTTGSGFLGGIIAGFFAGYLMLY
CCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
SKKLLSGLPKQFEGLKSIFILPIIGILITGVLMTLLGEPVAALNKGVMAWLASLEQANPI
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCH
LLGIIIGCMSAFDFGGPVNKAAYLTGTMLLAQGNYYFMAGVSAACITPPLIIALATTFFK
HHHHHHHHHHHHCCCCCCCHHHHHHHEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHH
NRFDEEERAAGLVNYILGCTHITEGAIPFAAKDPLRVIPILMVGSSVSSVLTYLMKIQVP
HHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCC
APHGGFLILPLVSKPLLWVAAILVGSTVGAILYGIYRGHTQKKAQ
CCCCCEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKKIIAVTGCPTGIAHTFMAEEALKKAAKELGIDIKVETNGAVGVENELTEKDIEEAIGV
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHCE
IVASDKDVRADRFTGRPTIETSVADGIRKPKELIEAILDGKAPIKGGAAAKKTIKKSLEE
EEECCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
VPGKKSVTRDIYKHLMNGVSHMLPFVVAGGVLIAISFLWGIYSFEPKSAQYNPIAAMIKG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHC
IGGEAFAIIVPIFTAYIADSISGRPGMVAGFVGGLIANTTGSGFLGGIIAGFFAGYLMLY
CCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
SKKLLSGLPKQFEGLKSIFILPIIGILITGVLMTLLGEPVAALNKGVMAWLASLEQANPI
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCH
LLGIIIGCMSAFDFGGPVNKAAYLTGTMLLAQGNYYFMAGVSAACITPPLIIALATTFFK
HHHHHHHHHHHHCCCCCCCHHHHHHHEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHH
NRFDEEERAAGLVNYILGCTHITEGAIPFAAKDPLRVIPILMVGSSVSSVLTYLMKIQVP
HHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCC
APHGGFLILPLVSKPLLWVAAILVGSTVGAILYGIYRGHTQKKAQ
CCCCCEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]