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The map label for this gene is fruA [H]

Identifier: 226949288

GI number: 226949288

Start: 2274546

End: 2274986

Strand: Reverse

Name: fruA [H]

Synonym: CLM_2207

Alternate gene names: 226949288

Gene position: 2274986-2274546 (Counterclockwise)

Preceding gene: 226949289

Following gene: 226949283

Centisome position: 54.75

GC content: 29.93

Gene sequence:

>441_bases
ATGGAACTAAATGAATTAATAACAGAAAGCACTATAGAATTAAATGTAGTGGCAAAGGATAAAGAGGACCTTATTAATAA
AATGATTGATGTTTTGGTAAAGGATGGGGCAGTTCTTGATGAAGATAAATTTAAAGAGGATATCTATCATAGGGAATCTT
TAAGTAATACAGGTATAGGCTTTGGAATTGCTATACCACATGCTAAATCTATAGCTGTAAAGGAACCTCGCATTGCAGTA
GGGATACTTAAAGATACTGTGGATTATGGCTCAATAGATGGAGAGCCAGTAAATATGATATTTATGATAGCAGTAAATGA
TATGCAAAGTGATCTTCATTTAAAAGCATTAGCTAACTTATCAAGAAAATTAATGCATGAAGATTTCAGAGAAAAGATCT
TAAATGCAAAATCAAAATTTGAAATATTAGAAATAATTTAA

Upstream 100 bases:

>100_bases
GTTAATTATAACTTATAATAGCATTGGACATTATTGTTAAACCTAAGACAAAAACAATGATCTACAAGGTATTTAGGATA
AGGGGATGAATAAAATAATT

Downstream 100 bases:

>100_bases
GTCGCTTATAAACAGAGTTTTGGGCTTCAGAGGGAGTTTTTACTTCCAATAAAGCTTAGGAAATGGTTATCCAGGGACAT
AGTCGCTCTTTACTCCTACT

Product: PTS system fructose family transporter subunit IIA

Products: NA

Alternate protein names: EIIABC-Fru; Fructose-specific phosphotransferase enzyme IIA component; EII-Fru; PTS system fructose-specific EIIA component; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MELNELITESTIELNVVAKDKEDLINKMIDVLVKDGAVLDEDKFKEDIYHRESLSNTGIGFGIAIPHAKSIAVKEPRIAV
GILKDTVDYGSIDGEPVNMIFMIAVNDMQSDLHLKALANLSRKLMHEDFREKILNAKSKFEILEII

Sequences:

>Translated_146_residues
MELNELITESTIELNVVAKDKEDLINKMIDVLVKDGAVLDEDKFKEDIYHRESLSNTGIGFGIAIPHAKSIAVKEPRIAV
GILKDTVDYGSIDGEPVNMIFMIAVNDMQSDLHLKALANLSRKLMHEDFREKILNAKSKFEILEII
>Mature_146_residues
MELNELITESTIELNVVAKDKEDLINKMIDVLVKDGAVLDEDKFKEDIYHRESLSNTGIGFGIAIPHAKSIAVKEPRIAV
GILKDTVDYGSIDGEPVNMIFMIAVNDMQSDLHLKALANLSRKLMHEDFREKILNAKSKFEILEII

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI48994992, Length=142, Percent_Identity=31.6901408450704, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI2367327, Length=123, Percent_Identity=28.4552845528455, Blast_Score=81, Evalue=3e-17,
Organism=Escherichia coli, GI1786951, Length=148, Percent_Identity=29.7297297297297, Blast_Score=80, Evalue=4e-17,
Organism=Escherichia coli, GI1788726, Length=127, Percent_Identity=29.9212598425197, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR004715
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF00359 PTS_EIIA_2; PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 16408; Mature: 16408

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELNELITESTIELNVVAKDKEDLINKMIDVLVKDGAVLDEDKFKEDIYHRESLSNTGIG
CCHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCE
FGIAIPHAKSIAVKEPRIAVGILKDTVDYGSIDGEPVNMIFMIAVNDMQSDLHLKALANL
EEEECCCCCCEEECCCCEEEEHHHHCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHH
SRKLMHEDFREKILNAKSKFEILEII
HHHHHHHHHHHHHHCCHHHHHEEECC
>Mature Secondary Structure
MELNELITESTIELNVVAKDKEDLINKMIDVLVKDGAVLDEDKFKEDIYHRESLSNTGIG
CCHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCE
FGIAIPHAKSIAVKEPRIAVGILKDTVDYGSIDGEPVNMIFMIAVNDMQSDLHLKALANL
EEEECCCCCCEEECCCCEEEEHHHHCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHH
SRKLMHEDFREKILNAKSKFEILEII
HHHHHHHHHHHHHHCCHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]