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The map label for this gene is spo0A [H]

Identifier: 226949170

GI number: 226949170

Start: 2144531

End: 2145352

Strand: Reverse

Name: spo0A [H]

Synonym: CLM_2088

Alternate gene names: 226949170

Gene position: 2145352-2144531 (Counterclockwise)

Preceding gene: 226949171

Following gene: 226949169

Centisome position: 51.63

GC content: 31.63

Gene sequence:

>822_bases
ATGGAAGAAACAAAGATCAATGTTATCATCGCAGATGATAATAAGGAATTTTGTAACATATTAAGTGATTATTTACTAAA
TCAAAGAGACATAATGGTTACAGGTATAGCTAATGATGGAGTAGAGGCTCTTAAATTGGTGGAAGAAAAAAAGCCAGATT
TAATAATACTAGATATAATAATGCCTCACTTAGATGGATTAGGGGTTTTGGAAAAGTTAAATAGTATAGATATTACTCCT
ATTCCAAGGGTAATTGTTTTGTCTGCTGTAGGACAGGATAAGATTACTCAAAGAGCTATAAACTTAGGTGCAGATTATTA
TGTAGTTAAACCTTTTGATATGGATGTTTTTACTAAAAGAATAAGACAAATGTTTAACAATACCATAAGTAGTGAAGAAG
TTAGAAGACCAGTAGTAATGCCGGAAACCTGTTCACAAGAAAAGATTTCATTATCTAAAAATGAACCAGCAGATTTAGAG
CAAGAAATAACAGATATTATACATGAAATAGGCGTGCCAGCTCACATAAAAGGATATATGTATTTGAGAGAAGCTATAAC
TATGGTAGTTAATGACATGGAACTTTTATCCGCAGTAACAAAAGAATTATACCCTTCCATAGCTAAAAAATATAATACTA
CAGCAAGCAGAGTGGAAAGAGCTATAAGACACGCTATAGAAGTAGCTTGGTCAAGAGGTCAAGTTGAGACTATAAACAAT
ATATTTGGGTATACTATAAATAATGGGAAGGGAAAACCAACAAATTCTGAGTTTATTGCAATGGTAGCTGATAAATTAAG
GTTAAAAAATAGGGTTTGTTAG

Upstream 100 bases:

>100_bases
TTTATATATTTTTTCTCAAATAAGGAGGAATTAAATTTCTTTTGTCGAATAAATAATTTGTTAAGATATGGTAATTTTAT
CAAAAAGGAGAGTAGTTTAT

Downstream 100 bases:

>100_bases
AAAGTAATACCAATGGTTTGTGACCAGTTCGCTTTTTATGCAAAATCACAAATTGGCAATATATGTGTATTAATCTTCAA
AAAAATACTAATAGACTTTC

Product: sporulation transcription factor Spo0A

Products: protein L-glutamate+ methanol; methanol; Tapglu; methanol; Targlu; Trgglu; Tsrglu; NH3; protein-L-glutamate [C]

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MEETKINVIIADDNKEFCNILSDYLLNQRDIMVTGIANDGVEALKLVEEKKPDLIILDIIMPHLDGLGVLEKLNSIDITP
IPRVIVLSAVGQDKITQRAINLGADYYVVKPFDMDVFTKRIRQMFNNTISSEEVRRPVVMPETCSQEKISLSKNEPADLE
QEITDIIHEIGVPAHIKGYMYLREAITMVVNDMELLSAVTKELYPSIAKKYNTTASRVERAIRHAIEVAWSRGQVETINN
IFGYTINNGKGKPTNSEFIAMVADKLRLKNRVC

Sequences:

>Translated_273_residues
MEETKINVIIADDNKEFCNILSDYLLNQRDIMVTGIANDGVEALKLVEEKKPDLIILDIIMPHLDGLGVLEKLNSIDITP
IPRVIVLSAVGQDKITQRAINLGADYYVVKPFDMDVFTKRIRQMFNNTISSEEVRRPVVMPETCSQEKISLSKNEPADLE
QEITDIIHEIGVPAHIKGYMYLREAITMVVNDMELLSAVTKELYPSIAKKYNTTASRVERAIRHAIEVAWSRGQVETINN
IFGYTINNGKGKPTNSEFIAMVADKLRLKNRVC
>Mature_273_residues
MEETKINVIIADDNKEFCNILSDYLLNQRDIMVTGIANDGVEALKLVEEKKPDLIILDIIMPHLDGLGVLEKLNSIDITP
IPRVIVLSAVGQDKITQRAINLGADYYVVKPFDMDVFTKRIRQMFNNTISSEEVRRPVVMPETCSQEKISLSKNEPADLE
QEITDIIHEIGVPAHIKGYMYLREAITMVVNDMELLSAVTKELYPSIAKKYNTTASRVERAIRHAIEVAWSRGQVETINN
IFGYTINNGKGKPTNSEFIAMVADKLRLKNRVC

Specific function: May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the ex

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788192, Length=109, Percent_Identity=35.7798165137615, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788550, Length=114, Percent_Identity=35.9649122807018, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR014879
- InterPro:   IPR012052
- InterPro:   IPR011991
- ProDom:   PD010908 [H]

Pfam domain/function: PF00072 Response_reg; PF08769 Spo0A_C [H]

EC number: 3.1.1.61 [C]

Molecular weight: Translated: 30820; Mature: 30820

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEETKINVIIADDNKEFCNILSDYLLNQRDIMVTGIANDGVEALKLVEEKKPDLIILDII
CCCCEEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEEEH
MPHLDGLGVLEKLNSIDITPIPRVIVLSAVGQDKITQRAINLGADYYVVKPFDMDVFTKR
HHCCCCCHHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCHHHHHHH
IRQMFNNTISSEEVRRPVVMPETCSQEKISLSKNEPADLEQEITDIIHEIGVPAHIKGYM
HHHHHCCCCCHHHHCCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
YLREAITMVVNDMELLSAVTKELYPSIAKKYNTTASRVERAIRHAIEVAWSRGQVETINN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHH
IFGYTINNGKGKPTNSEFIAMVADKLRLKNRVC
HHCEEEECCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEETKINVIIADDNKEFCNILSDYLLNQRDIMVTGIANDGVEALKLVEEKKPDLIILDII
CCCCEEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEEEH
MPHLDGLGVLEKLNSIDITPIPRVIVLSAVGQDKITQRAINLGADYYVVKPFDMDVFTKR
HHCCCCCHHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCHHHHHHH
IRQMFNNTISSEEVRRPVVMPETCSQEKISLSKNEPADLEQEITDIIHEIGVPAHIKGYM
HHHHHCCCCCHHHHCCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
YLREAITMVVNDMELLSAVTKELYPSIAKKYNTTASRVERAIRHAIEVAWSRGQVETINN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHH
IFGYTINNGKGKPTNSEFIAMVADKLRLKNRVC
HHCEEEECCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein L-glutamate Omethyl ester; Tapglu-Me; H2O; Targlu-Me; Trgglu-Me; Tsrglu-Me; Tsrgln; Trggln; Targln; Tapgln; protein-L-glutamine [C]

Specific reaction: Protein L-glutamate Omethyl ester + H2O = protein L-glutamate+ methanol H2O + Tapglu-Me = methanol + Tapglu H2O + Targlu-Me = methanol + Targlu H2O + Trgglu-Me = methanol + Trgglu H2O + Tsrglu-Me = methanol + Tsrglu H2O + Tsrgln = NH3 + Tsrglu H2O + Trggl

General reaction: Hydrolysis of carboxylic ester [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]