Click here to switch to the map view.

The map label for this gene is ogt [H]

Identifier: 226948864

GI number: 226948864

Start: 1805501

End: 1805971

Strand: Direct

Name: ogt [H]

Synonym: CLM_1766

Alternate gene names: 226948864

Gene position: 1805501-1805971 (Clockwise)

Preceding gene: 226948863

Following gene: 226948865

Centisome position: 43.45

GC content: 33.12

Gene sequence:

>471_bases
ATGAATAATGCATATTGTTATGAAACCAAAATTGGGAAAATAGTAATTGTCGAAAATGGGACATCTATTACTCAGCTGTG
TTTTGGAGAAGATATTCCTAAAGATGTAACCATTCTTGAGACTGATTTACTAAAAGAAGCAAATAAAGAGCTTGAAGAAT
ATTTTAGAGGAAAAAGAGAAAAGTTTGATTTATCCTTTGATCCACAAGGTACGGAATTTCAAAAAAAGGTTTGGAAGGCG
TTACAAGAAATACCCTATGGGAAAACTTATAGCTACAAAAATGTAGCAGTAAATATAGGCAATGAGAAGGCTTGTCGTGC
GGTAGGTATGGCCAACAATAAGAATCCTATACCTATTTTTATTCCTTGTCATCGTGTTATTGGAGCTAATGGTAGTTTAG
TTGGTTATGCAGGTGGACTAGATGTAAAAGAGAAACTTTTAAAAATAGAAAAAGAGAACTCAAAAGGGTAA

Upstream 100 bases:

>100_bases
GTGGATGGCAAGTTAATTATATTTTTTGAAGGTGCTGAAGGGGAGAAAATTGAACTAGTTCAGTACATATAGAAATATAT
TATATGGAGGAGAAGGGCAT

Downstream 100 bases:

>100_bases
AACAAAACCACCCAAAACATACTGAATTGTTAAAAGAGATAAGGGGCTTAGAAGGTATGAAAAGGAAAAAAATACTAGAA
AATCTGAAATCACAAATAAA

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MNNAYCYETKIGKIVIVENGTSITQLCFGEDIPKDVTILETDLLKEANKELEEYFRGKREKFDLSFDPQGTEFQKKVWKA
LQEIPYGKTYSYKNVAVNIGNEKACRAVGMANNKNPIPIFIPCHRVIGANGSLVGYAGGLDVKEKLLKIEKENSKG

Sequences:

>Translated_156_residues
MNNAYCYETKIGKIVIVENGTSITQLCFGEDIPKDVTILETDLLKEANKELEEYFRGKREKFDLSFDPQGTEFQKKVWKA
LQEIPYGKTYSYKNVAVNIGNEKACRAVGMANNKNPIPIFIPCHRVIGANGSLVGYAGGLDVKEKLLKIEKENSKG
>Mature_156_residues
MNNAYCYETKIGKIVIVENGTSITQLCFGEDIPKDVTILETDLLKEANKELEEYFRGKREKFDLSFDPQGTEFQKKVWKA
LQEIPYGKTYSYKNVAVNIGNEKACRAVGMANNKNPIPIFIPCHRVIGANGSLVGYAGGLDVKEKLLKIEKENSKG

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=151, Percent_Identity=35.7615894039735, Blast_Score=82, Evalue=2e-16,
Organism=Escherichia coli, GI1787596, Length=104, Percent_Identity=49.0384615384615, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI1788542, Length=157, Percent_Identity=33.7579617834395, Blast_Score=98, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI115533070, Length=80, Percent_Identity=46.25, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI115533068, Length=80, Percent_Identity=46.25, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320001, Length=85, Percent_Identity=43.5294117647059, Blast_Score=83, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 17482; Mature: 17482

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNAYCYETKIGKIVIVENGTSITQLCFGEDIPKDVTILETDLLKEANKELEEYFRGKRE
CCCCEEEEECCCEEEEEECCCCEEHEECCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCE
KFDLSFDPQGTEFQKKVWKALQEIPYGKTYSYKNVAVNIGNEKACRAVGMANNKNPIPIF
EECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHEECCCCCCCCCEEE
IPCHRVIGANGSLVGYAGGLDVKEKLLKIEKENSKG
EECHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNNAYCYETKIGKIVIVENGTSITQLCFGEDIPKDVTILETDLLKEANKELEEYFRGKRE
CCCCEEEEECCCEEEEEECCCCEEHEECCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCE
KFDLSFDPQGTEFQKKVWKALQEIPYGKTYSYKNVAVNIGNEKACRAVGMANNKNPIPIF
EECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHEECCCCCCCCCEEE
IPCHRVIGANGSLVGYAGGLDVKEKLLKIEKENSKG
EECHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA