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The map label for this gene is dacF [H]

Identifier: 226948683

GI number: 226948683

Start: 1641348

End: 1642622

Strand: Reverse

Name: dacF [H]

Synonym: CLM_1581

Alternate gene names: 226948683

Gene position: 1642622-1641348 (Counterclockwise)

Preceding gene: 226948685

Following gene: 226948669

Centisome position: 39.53

GC content: 28.0

Gene sequence:

>1275_bases
ATGAGAAAAGGAAAAACGACTTTAATTTCACTATTAATTTTTATTCTCACCTTAACATCTAATGTATTTGCTGCAGGAGA
TTCAAATACTGAGCCCCCTACTATTTATGGTAAGACAGCTATGACTATAGATGTTGAAACTGGTGAAATAATATATGCTA
AAAATATTGACAAGCGTATGTATCCTGCTAGTACTACAAAATATTTAACAGCCCTTTTATTGGCTGAAAACAAAGGAAAA
AATGATAATATACCTTATACAGCTACTGCTAAAAGTCAACCGCAATATTCTCTAAATATAAATCTTCACCCTATAGATAT
AGGAGAAACTATAAAAGCAGAAAATGTTATGGACGGATTATTACTTTTTTCTGGTAATGATGTAGCTTATATGATAGGTG
ATGCTTTAGCTGGTAATAATAAAAATTTTGAAAATATGATGAATAAAAAGATACAAGAGTTAAATTTAAAGAACACTCAC
TTTGTAACCCCTAATGGATTACATAATCCTAATCATTATACTACAGCTTATGATTTAAGTGTAATTAGTAGGGCTGCTCT
AAAAAATCCTTGGGTTTACCAATCTTCTAATAAAAAAGAAAGCTCTATAGTCACTTCTAAAGGTACAAAAATGGCTATAG
AAAATAGTAATAAGCTTTTAGGGAAAGATGGTTGTGTTGCAGGTAAAACAGGTTACACTGATGCTGCTGGTAGATGTTTA
GTAACTTTGTTTAATAGAGATGGTAGAAAAATACTTGGGGTAGTTATGGGATCTGTATATGATGCTAAAGATTCTTTCGT
TTTTCAAGATATGAAAAAAATTATAGATTGGAGTTATAATGTAAAACCATCCACTCTATATAAAAAAGACTCTAGCCTAA
AAACAGAAACAGTAACCTATAATCCTTTAAAAATTATAGGACCTTCTGTGACTATAGATGTGCCTATAATTTTAAAAGAA
GATGTTACTTACTATAAAAATGAAGTAAATGATAAAGAAGCTAATAAAACAATCAATATAGACAATACTTCTTATGCTGC
TCTTTCTGGCAAAAAAAATATGGGAACTTTAACTGTAAAAGAAAGAGAATACGAAAAAAAATATGAAGTATACTCTGGTT
TACCTAAAGAAGAAATAATGAAAAAGAATATGCCTTTTTATGTGGTTTCTATAATTTTAGTACTAGCTATAATTACTGTA
ATATTCTTTTTAGTAAAATTTTTTAAAAATAAGATTAACAAAAAAAATAGAAATAAAAATAAATACTGGTATTAA

Upstream 100 bases:

>100_bases
AAAATATACTCTCTAATGCTATACTATCAAAGTGTATACATATAGTATATACTTAAAATTTTATATTTAAATTAGAAAAA
AGTAAATGGAGAGATGGCCT

Downstream 100 bases:

>100_bases
AATATCAATATTATTTACATCATAAAAAACTTGCTAATATACTTTAAATATATAATAAAAACACTATCAAAATATAAGTG
ATAGTGTTTTTATTATATAC

Product: D-alanyl-D-alanine carboxypeptidase family protein

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MRKGKTTLISLLIFILTLTSNVFAAGDSNTEPPTIYGKTAMTIDVETGEIIYAKNIDKRMYPASTTKYLTALLLAENKGK
NDNIPYTATAKSQPQYSLNINLHPIDIGETIKAENVMDGLLLFSGNDVAYMIGDALAGNNKNFENMMNKKIQELNLKNTH
FVTPNGLHNPNHYTTAYDLSVISRAALKNPWVYQSSNKKESSIVTSKGTKMAIENSNKLLGKDGCVAGKTGYTDAAGRCL
VTLFNRDGRKILGVVMGSVYDAKDSFVFQDMKKIIDWSYNVKPSTLYKKDSSLKTETVTYNPLKIIGPSVTIDVPIILKE
DVTYYKNEVNDKEANKTINIDNTSYAALSGKKNMGTLTVKEREYEKKYEVYSGLPKEEIMKKNMPFYVVSIILVLAIITV
IFFLVKFFKNKINKKNRNKNKYWY

Sequences:

>Translated_424_residues
MRKGKTTLISLLIFILTLTSNVFAAGDSNTEPPTIYGKTAMTIDVETGEIIYAKNIDKRMYPASTTKYLTALLLAENKGK
NDNIPYTATAKSQPQYSLNINLHPIDIGETIKAENVMDGLLLFSGNDVAYMIGDALAGNNKNFENMMNKKIQELNLKNTH
FVTPNGLHNPNHYTTAYDLSVISRAALKNPWVYQSSNKKESSIVTSKGTKMAIENSNKLLGKDGCVAGKTGYTDAAGRCL
VTLFNRDGRKILGVVMGSVYDAKDSFVFQDMKKIIDWSYNVKPSTLYKKDSSLKTETVTYNPLKIIGPSVTIDVPIILKE
DVTYYKNEVNDKEANKTINIDNTSYAALSGKKNMGTLTVKEREYEKKYEVYSGLPKEEIMKKNMPFYVVSIILVLAIITV
IFFLVKFFKNKINKKNRNKNKYWY
>Mature_424_residues
MRKGKTTLISLLIFILTLTSNVFAAGDSNTEPPTIYGKTAMTIDVETGEIIYAKNIDKRMYPASTTKYLTALLLAENKGK
NDNIPYTATAKSQPQYSLNINLHPIDIGETIKAENVMDGLLLFSGNDVAYMIGDALAGNNKNFENMMNKKIQELNLKNTH
FVTPNGLHNPNHYTTAYDLSVISRAALKNPWVYQSSNKKESSIVTSKGTKMAIENSNKLLGKDGCVAGKTGYTDAAGRCL
VTLFNRDGRKILGVVMGSVYDAKDSFVFQDMKKIIDWSYNVKPSTLYKKDSSLKTETVTYNPLKIIGPSVTIDVPIILKE
DVTYYKNEVNDKEANKTINIDNTSYAALSGKKNMGTLTVKEREYEKKYEVYSGLPKEEIMKKNMPFYVVSIILVLAIITV
IFFLVKFFKNKINKKNRNKNKYWY

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=311, Percent_Identity=28.2958199356913, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787062, Length=305, Percent_Identity=26.5573770491803, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786851, Length=258, Percent_Identity=29.4573643410853, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI87082054, Length=254, Percent_Identity=28.3464566929134, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 47619; Mature: 47619

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKGKTTLISLLIFILTLTSNVFAAGDSNTEPPTIYGKTAMTIDVETGEIIYAKNIDKRM
CCCCHHHHHHHHHHHHHHHCCEEECCCCCCCCCEEECCEEEEEEECCCCEEEEECCCCCC
YPASTTKYLTALLLAENKGKNDNIPYTATAKSQPQYSLNINLHPIDIGETIKAENVMDGL
CCCCHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCCCCCCHHHHHCEE
LLFSGNDVAYMIGDALAGNNKNFENMMNKKIQELNLKNTHFVTPNGLHNPNHYTTAYDLS
EEEECCCEEEEEECHHCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEEEEHHH
VISRAALKNPWVYQSSNKKESSIVTSKGTKMAIENSNKLLGKDGCVAGKTGYTDAAGRCL
HHHHHHHCCCCEEECCCCCCCCEEECCCCEEEEECCCCEECCCCCCCCCCCCCCCCCCEE
VTLFNRDGRKILGVVMGSVYDAKDSFVFQDMKKIIDWSYNVKPSTLYKKDSSLKTETVTY
EEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEECCCCCEEEEEEE
NPLKIIGPSVTIDVPIILKEDVTYYKNEVNDKEANKTINIDNTSYAALSGKKNMGTLTVK
CCEEEECCCEEEEEEEEEECCCHHHHHCCCCCCCCCEEEECCCCEEEECCCCCCCEEEEE
EREYEKKYEVYSGLPKEEIMKKNMPFYVVSIILVLAIITVIFFLVKFFKNKINKKNRNKN
HHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KYWY
CCCC
>Mature Secondary Structure
MRKGKTTLISLLIFILTLTSNVFAAGDSNTEPPTIYGKTAMTIDVETGEIIYAKNIDKRM
CCCCHHHHHHHHHHHHHHHCCEEECCCCCCCCCEEECCEEEEEEECCCCEEEEECCCCCC
YPASTTKYLTALLLAENKGKNDNIPYTATAKSQPQYSLNINLHPIDIGETIKAENVMDGL
CCCCHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCCCCCCHHHHHCEE
LLFSGNDVAYMIGDALAGNNKNFENMMNKKIQELNLKNTHFVTPNGLHNPNHYTTAYDLS
EEEECCCEEEEEECHHCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEEEEHHH
VISRAALKNPWVYQSSNKKESSIVTSKGTKMAIENSNKLLGKDGCVAGKTGYTDAAGRCL
HHHHHHHCCCCEEECCCCCCCCEEECCCCEEEEECCCCEECCCCCCCCCCCCCCCCCCEE
VTLFNRDGRKILGVVMGSVYDAKDSFVFQDMKKIIDWSYNVKPSTLYKKDSSLKTETVTY
EEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEECCCCCEEEEEEE
NPLKIIGPSVTIDVPIILKEDVTYYKNEVNDKEANKTINIDNTSYAALSGKKNMGTLTVK
CCEEEECCCEEEEEEEEEECCCHHHHHCCCCCCCCCEEEECCCCEEEECCCCCCCEEEEE
EREYEKKYEVYSGLPKEEIMKKNMPFYVVSIILVLAIITVIFFLVKFFKNKINKKNRNKN
HHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KYWY
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]