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The map label for this gene is gltX

Identifier: 226948369

GI number: 226948369

Start: 1304610

End: 1306067

Strand: Reverse

Name: gltX

Synonym: CLM_1252

Alternate gene names: 226948369

Gene position: 1306067-1304610 (Counterclockwise)

Preceding gene: 226948371

Following gene: 226948355

Centisome position: 31.43

GC content: 30.32

Gene sequence:

>1458_bases
ATGACAAACAAAGTCAGAACTAGATTCGCACCAAGTCCAACAGGTTATATGCATGTTGGTAACTTAAGAACTGCTTTATA
TGCTTATTTAATAGCAAAGCATGATAATGGTGATTTTATATTAAGAATTGAGGATACAGATCAAGAAAGATTAGTAGAAG
GTGCTTTAGATGTTATATATAATACTCTAAAAATTACTGGTCTTAGTCATGATGAAGGTCCAGATATTGGCGGACCTGTT
GGTCCATATGTTCAAAGTGAAAGAAGAAATATATACATAGAATATGCTGAAAAGCTAATAGAAAAAGGAGAAGCATACTA
CTGTTTTTGTTCAAAAGAAAGATTAGATATGCTAAGAGCTAATTCAGAGGCTTTAAAAAGACCTTTCAGATATGACAAAC
ACTGCATAGATCTTTCTAAAGAAGAAATCGATAAAAAAATAGCTGAAGGTGTTCCATATGTTATAAGACAAAAAAATCCA
ACTACTGGATCAACTTCTTTCCATGATGAAATATATGGAGATATTTCCGTAGATAATTCAGAATTAGATGATATGATTTT
AATAAAATCGGATGGACTTCCAACATATAATTTTGCTAATGTAGTAGATGACCATCTTATGGGAATAACTCACGTAGTAC
GTGGAAGTGAATATTTGTCATCTTCTCCTAAATATAATAGACTATATGAAGCTTTTGGCTGGGATGTTCCGATATACGTT
CATTGCCCACCAATAATGAAAGATGAGCATCACAAATTAAGTAAGAGAAATGGAGATGCTTCTTTCGAAGATTTAATGGC
TAAAGGTTACTTAAAAGAAGCTATCCTAAACTATATAGCTTTACTGGGATGGAACCCAGGTGGAGAAAAAGAAGTATTTT
CTATGGAAGAATTAATAGAAGCCTTTAATTATAGAAATATAAATAAAGCTCCAGCAGTTTTTGATACAAAAAAATTAAAA
TGGATGAACGGAGAATATATAAGAGCTTTATCTTTAGATAAATTTCATGAAATGGCTCTTCCTTATTATGAAGAAGCTTT
GACTAGAGATTTAGATACTAAAAAAATAAGTGAACTTTTACACACTAGAGTAGAGGTTTTAAATGAAATTCCTGAACAAT
TAGATTTCTTTAATAATCTTCTTGAATACTCTCCGGAAATGTATATTCATAAAAAAATGAAAACAACTTATGAAAATTCA
TTAAAAAGCTTAGAAGAAGTTCTTCCTAAACTAGAAGCTCTAGAAAATTGGACTTTTGAAAATATTAAAGAAGTATGTAT
GAATTTAGTTAAAGAACTTGAAGTTAAAAACGGTGTAGTTTTATGGCCTATAAGAACAGCAGTTTCTGGAAAACAATTTA
CTCCTGGTGGAGCTTTTGAAATAGCTGATATATTAGGTAAGGAAGAAACTCTAGAAAGAATAAAAATAGGAATAGACAAA
CTTAAAGCTTTACAATAA

Upstream 100 bases:

>100_bases
AGGCCTTGTTATAAAATTTACTTATAAAATGAATTTTTACCTTTCGTCCAAGTGATGAAAGGTTTTATTTATAAATATTT
TTTAAATGGGAGTGAAAACA

Downstream 100 bases:

>100_bases
AATTATATTTTACACTTAAAAATTGTAATAAATATACACTAAGAAAAATCTCAGCTAATTATAGCTGAGATTTTATTTTT
TTATTTTTTTTATTTTTTTT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MTNKVRTRFAPSPTGYMHVGNLRTALYAYLIAKHDNGDFILRIEDTDQERLVEGALDVIYNTLKITGLSHDEGPDIGGPV
GPYVQSERRNIYIEYAEKLIEKGEAYYCFCSKERLDMLRANSEALKRPFRYDKHCIDLSKEEIDKKIAEGVPYVIRQKNP
TTGSTSFHDEIYGDISVDNSELDDMILIKSDGLPTYNFANVVDDHLMGITHVVRGSEYLSSSPKYNRLYEAFGWDVPIYV
HCPPIMKDEHHKLSKRNGDASFEDLMAKGYLKEAILNYIALLGWNPGGEKEVFSMEELIEAFNYRNINKAPAVFDTKKLK
WMNGEYIRALSLDKFHEMALPYYEEALTRDLDTKKISELLHTRVEVLNEIPEQLDFFNNLLEYSPEMYIHKKMKTTYENS
LKSLEEVLPKLEALENWTFENIKEVCMNLVKELEVKNGVVLWPIRTAVSGKQFTPGGAFEIADILGKEETLERIKIGIDK
LKALQ

Sequences:

>Translated_485_residues
MTNKVRTRFAPSPTGYMHVGNLRTALYAYLIAKHDNGDFILRIEDTDQERLVEGALDVIYNTLKITGLSHDEGPDIGGPV
GPYVQSERRNIYIEYAEKLIEKGEAYYCFCSKERLDMLRANSEALKRPFRYDKHCIDLSKEEIDKKIAEGVPYVIRQKNP
TTGSTSFHDEIYGDISVDNSELDDMILIKSDGLPTYNFANVVDDHLMGITHVVRGSEYLSSSPKYNRLYEAFGWDVPIYV
HCPPIMKDEHHKLSKRNGDASFEDLMAKGYLKEAILNYIALLGWNPGGEKEVFSMEELIEAFNYRNINKAPAVFDTKKLK
WMNGEYIRALSLDKFHEMALPYYEEALTRDLDTKKISELLHTRVEVLNEIPEQLDFFNNLLEYSPEMYIHKKMKTTYENS
LKSLEEVLPKLEALENWTFENIKEVCMNLVKELEVKNGVVLWPIRTAVSGKQFTPGGAFEIADILGKEETLERIKIGIDK
LKALQ
>Mature_484_residues
TNKVRTRFAPSPTGYMHVGNLRTALYAYLIAKHDNGDFILRIEDTDQERLVEGALDVIYNTLKITGLSHDEGPDIGGPVG
PYVQSERRNIYIEYAEKLIEKGEAYYCFCSKERLDMLRANSEALKRPFRYDKHCIDLSKEEIDKKIAEGVPYVIRQKNPT
TGSTSFHDEIYGDISVDNSELDDMILIKSDGLPTYNFANVVDDHLMGITHVVRGSEYLSSSPKYNRLYEAFGWDVPIYVH
CPPIMKDEHHKLSKRNGDASFEDLMAKGYLKEAILNYIALLGWNPGGEKEVFSMEELIEAFNYRNINKAPAVFDTKKLKW
MNGEYIRALSLDKFHEMALPYYEEALTRDLDTKKISELLHTRVEVLNEIPEQLDFFNNLLEYSPEMYIHKKMKTTYENSL
KSLEEVLPKLEALENWTFENIKEVCMNLVKELEVKNGVVLWPIRTAVSGKQFTPGGAFEIADILGKEETLERIKIGIDKL
KALQ

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=487, Percent_Identity=34.7022587268994, Blast_Score=268, Evalue=1e-71,
Organism=Escherichia coli, GI1788743, Length=483, Percent_Identity=37.888198757764, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI226510925, Length=295, Percent_Identity=33.8983050847458, Blast_Score=157, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71993394, Length=491, Percent_Identity=36.8635437881874, Blast_Score=263, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6324540, Length=502, Percent_Identity=40.2390438247012, Blast_Score=323, Evalue=3e-89,
Organism=Saccharomyces cerevisiae, GI6321192, Length=240, Percent_Identity=25, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24665321, Length=493, Percent_Identity=36.105476673428, Blast_Score=304, Evalue=1e-82,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_CLOB1 (A7FSY9)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001383466.1
- ProteinModelPortal:   A7FSY9
- SMR:   A7FSY9
- STRING:   A7FSY9
- GeneID:   5396897
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_1134
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- ProtClustDB:   PRK01406
- BioCyc:   CBOT441770:CLB_1134-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 55743; Mature: 55612

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 255-255

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNKVRTRFAPSPTGYMHVGNLRTALYAYLIAKHDNGDFILRIEDTDQERLVEGALDVIY
CCCCCCCCCCCCCCCEEEECHHHHHHHHHHHEEECCCCEEEEEECCCHHHHHHHHHHHHH
NTLKITGLSHDEGPDIGGPVGPYVQSERRNIYIEYAEKLIEKGEAYYCFCSKERLDMLRA
HHHHEEECCCCCCCCCCCCCCCCHHCCCCEEEHHHHHHHHHCCCEEEEEECHHHHHHHHC
NSEALKRPFRYDKHCIDLSKEEIDKKIAEGVPYVIRQKNPTTGSTSFHDEIYGDISVDNS
CHHHHHCCHHCCHHHHCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHCCCEECCCC
ELDDMILIKSDGLPTYNFANVVDDHLMGITHVVRGSEYLSSSPKYNRLYEAFGWDVPIYV
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHCCCCEEEE
HCPPIMKDEHHKLSKRNGDASFEDLMAKGYLKEAILNYIALLGWNPGGEKEVFSMEELIE
ECCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
AFNYRNINKAPAVFDTKKLKWMNGEYIRALSLDKFHEMALPYYEEALTRDLDTKKISELL
HHCCCCCCCCCCCCCCCCEEECCCCEEEEECHHHHHHHHCHHHHHHHHCCCCHHHHHHHH
HTRVEVLNEIPEQLDFFNNLLEYSPEMYIHKKMKTTYENSLKSLEEVLPKLEALENWTFE
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
NIKEVCMNLVKELEVKNGVVLWPIRTAVSGKQFTPGGAFEIADILGKEETLERIKIGIDK
HHHHHHHHHHHHHHHCCCEEEEEEHHHCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHH
LKALQ
HHCCC
>Mature Secondary Structure 
TNKVRTRFAPSPTGYMHVGNLRTALYAYLIAKHDNGDFILRIEDTDQERLVEGALDVIY
CCCCCCCCCCCCCCEEEECHHHHHHHHHHHEEECCCCEEEEEECCCHHHHHHHHHHHHH
NTLKITGLSHDEGPDIGGPVGPYVQSERRNIYIEYAEKLIEKGEAYYCFCSKERLDMLRA
HHHHEEECCCCCCCCCCCCCCCCHHCCCCEEEHHHHHHHHHCCCEEEEEECHHHHHHHHC
NSEALKRPFRYDKHCIDLSKEEIDKKIAEGVPYVIRQKNPTTGSTSFHDEIYGDISVDNS
CHHHHHCCHHCCHHHHCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHCCCEECCCC
ELDDMILIKSDGLPTYNFANVVDDHLMGITHVVRGSEYLSSSPKYNRLYEAFGWDVPIYV
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHCCCCEEEE
HCPPIMKDEHHKLSKRNGDASFEDLMAKGYLKEAILNYIALLGWNPGGEKEVFSMEELIE
ECCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
AFNYRNINKAPAVFDTKKLKWMNGEYIRALSLDKFHEMALPYYEEALTRDLDTKKISELL
HHCCCCCCCCCCCCCCCCEEECCCCEEEEECHHHHHHHHCHHHHHHHHCCCCHHHHHHHH
HTRVEVLNEIPEQLDFFNNLLEYSPEMYIHKKMKTTYENSLKSLEEVLPKLEALENWTFE
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
NIKEVCMNLVKELEVKNGVVLWPIRTAVSGKQFTPGGAFEIADILGKEETLERIKIGIDK
HHHHHHHHHHHHHHHCCCEEEEEEHHHCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHH
LKALQ
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA