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The map label for this gene is chiA [H]

Identifier: 226948358

GI number: 226948358

Start: 1292856

End: 1294439

Strand: Direct

Name: chiA [H]

Synonym: CLM_1240

Alternate gene names: 226948358

Gene position: 1292856-1294439 (Clockwise)

Preceding gene: 226948357

Following gene: 226948359

Centisome position: 31.11

GC content: 35.48

Gene sequence:

>1584_bases
ATGAAATCACGAAAAATTACTGCAATTATTTTATCCGTATTGGTAGCTTCAGGAGCCACTGTATCAGTTTTTACAGGGAA
AAATGTAGTACAAGCTAAAGAGTTAGAAATTAACAGTGTACAATCTAAAACAGCTACTAATATAGGAGTGGGACAAGAAT
ATTGGGGATCTACTGTTTTCGCTCCTTATGTAGATGCTTGTTCATATCCTGTGTTTTCACTTACTGAATATGCAAAGGCA
ACAGGAACTAAACATTTTATATTAGGATTTGTAGTTGATAAAAATGGACAACCTTCCTGGGGAACTTATTATGATATGGA
AGAGGGGCCTACTGACTATCAAGGTGGAGGATTATTAAAAGAAATTAAAGCTTTAAGACAAATGGGCGGAGACGTAATGG
TCTCTTTTGGTGGAGAAGCTAATACTCCGTTAGCAGCATCAATTAAGGATGTTAATAAATTAAAAGATGCATATAAGAAA
ATAATCAAAGATTATGGATTAACTCATGTAGATTTTGACATTGAAGGAGCCTGGACAGCTGATGCCGCTTCTATAGAAAG
ACGTTCACAAGCCATTGCATTACTTCAAAAGGAACTTAAAGCTGAAAATCATCCACTTGAAATATGGTATACATTACCTG
TATTGCCTACAGGAATGACTAATGGTGTAGATGTAGTAAAAAATGCTGTAAAAAACGGTGTTCAGTTAAGTGGAGTAAAT
GTAATGACTATGTGTTTTGGACAGAAGGCTCCAGATGGAACTATGGGGGATTTAGTTATACAATCAGCTAAAAGCGTTCA
TAAACAATTAAAGGATATATACACACAAGCTAATATACAAAAGACTGATAAAGAAATTTGGAAAATGATAGGAGTAACTC
CTATGAATGGTTTAGATTATAGTGGAAGTATACTTGATCAAAATGGAGCAAGTAAGGTCGCGAGCTTTGGTAAAACAGTA
GGAATGGGACTTATAGGTATGTGGTCAGTAAATAGAGATTATCAAAATCCTAAGGGTTCAACAAATTACGTATCTATAAC
GGATAGTTCTATATTACAAGAAAAATTTGAATTTGGTAAAATACTTTCAAAATATGATTCAAATGATGGTACAGAAATAA
TAGAAAAGCCAGGTACAACTGATCCAGAAGATCCAGGCGATGGTGAAGATGGAGGAGAGACAGGGGAAATTGCTGCATAT
AATCCTAATACAGAATATTGGGCAGGAGATAAAGTTAGCTATAAAGGAAAGGTTTATCAAGCTAAATGGTGGACAAAAGG
GTTTGCACCAGATACGGCAGTACAAAATTCATGGGATACACCATGGGAACTAATAGGAGATTCAGATAATGATATAGAAA
TACCAGAAAAACCAGGTACAACTGATCCAGTGGATCCAGAGGATGGTAAAGATGGGGAACAGACAGGAGAAATCGCTGCA
TATACTCCTAATAAAGAATATTATGAAGGAGATAAAGTTACCTATAAAGGAAAGGTTTATCAAGCCAAATGGTGGACAAA
AGGGTTTGCACCAGATACAGTAGTAAAAAATACATGGGATACACCTTGGGAGCTAATAGGATAA

Upstream 100 bases:

>100_bases
ATCATTAGTGTGTTAATAGGAACTACGATACTTTCAACTGTTAGTTCCGTATCAACTTATGCAAAATCAAAAAAATATTA
ATAATATGGGGGTAGATACA

Downstream 100 bases:

>100_bases
GGTATAGTATTGATAAAATTAAATATATAAATTTTTCAAGAGTATTGGGTTAGAGGTATATTTCTCTCTAATCCAATACT
TTATTTTAACCAATAAACAT

Product: putative glycosyl hydrolase

Products: NA

Alternate protein names: Chitinase; Lysozyme [H]

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MKSRKITAIILSVLVASGATVSVFTGKNVVQAKELEINSVQSKTATNIGVGQEYWGSTVFAPYVDACSYPVFSLTEYAKA
TGTKHFILGFVVDKNGQPSWGTYYDMEEGPTDYQGGGLLKEIKALRQMGGDVMVSFGGEANTPLAASIKDVNKLKDAYKK
IIKDYGLTHVDFDIEGAWTADAASIERRSQAIALLQKELKAENHPLEIWYTLPVLPTGMTNGVDVVKNAVKNGVQLSGVN
VMTMCFGQKAPDGTMGDLVIQSAKSVHKQLKDIYTQANIQKTDKEIWKMIGVTPMNGLDYSGSILDQNGASKVASFGKTV
GMGLIGMWSVNRDYQNPKGSTNYVSITDSSILQEKFEFGKILSKYDSNDGTEIIEKPGTTDPEDPGDGEDGGETGEIAAY
NPNTEYWAGDKVSYKGKVYQAKWWTKGFAPDTAVQNSWDTPWELIGDSDNDIEIPEKPGTTDPVDPEDGKDGEQTGEIAA
YTPNKEYYEGDKVTYKGKVYQAKWWTKGFAPDTVVKNTWDTPWELIG

Sequences:

>Translated_527_residues
MKSRKITAIILSVLVASGATVSVFTGKNVVQAKELEINSVQSKTATNIGVGQEYWGSTVFAPYVDACSYPVFSLTEYAKA
TGTKHFILGFVVDKNGQPSWGTYYDMEEGPTDYQGGGLLKEIKALRQMGGDVMVSFGGEANTPLAASIKDVNKLKDAYKK
IIKDYGLTHVDFDIEGAWTADAASIERRSQAIALLQKELKAENHPLEIWYTLPVLPTGMTNGVDVVKNAVKNGVQLSGVN
VMTMCFGQKAPDGTMGDLVIQSAKSVHKQLKDIYTQANIQKTDKEIWKMIGVTPMNGLDYSGSILDQNGASKVASFGKTV
GMGLIGMWSVNRDYQNPKGSTNYVSITDSSILQEKFEFGKILSKYDSNDGTEIIEKPGTTDPEDPGDGEDGGETGEIAAY
NPNTEYWAGDKVSYKGKVYQAKWWTKGFAPDTAVQNSWDTPWELIGDSDNDIEIPEKPGTTDPVDPEDGKDGEQTGEIAA
YTPNKEYYEGDKVTYKGKVYQAKWWTKGFAPDTVVKNTWDTPWELIG
>Mature_527_residues
MKSRKITAIILSVLVASGATVSVFTGKNVVQAKELEINSVQSKTATNIGVGQEYWGSTVFAPYVDACSYPVFSLTEYAKA
TGTKHFILGFVVDKNGQPSWGTYYDMEEGPTDYQGGGLLKEIKALRQMGGDVMVSFGGEANTPLAASIKDVNKLKDAYKK
IIKDYGLTHVDFDIEGAWTADAASIERRSQAIALLQKELKAENHPLEIWYTLPVLPTGMTNGVDVVKNAVKNGVQLSGVN
VMTMCFGQKAPDGTMGDLVIQSAKSVHKQLKDIYTQANIQKTDKEIWKMIGVTPMNGLDYSGSILDQNGASKVASFGKTV
GMGLIGMWSVNRDYQNPKGSTNYVSITDSSILQEKFEFGKILSKYDSNDGTEIIEKPGTTDPEDPGDGEDGGETGEIAAY
NPNTEYWAGDKVSYKGKVYQAKWWTKGFAPDTAVQNSWDTPWELIGDSDNDIEIPEKPGTTDPVDPEDGKDGEQTGEIAA
YTPNKEYYEGDKVTYKGKVYQAKWWTKGFAPDTVVKNTWDTPWELIG

Specific function: Bifunctional enzyme with lysozyme/chitinase activity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 5 chitin-binding type-3 domains [H]

Homologues:

Organism=Escherichia coli, GI1789736, Length=337, Percent_Identity=37.9821958456973, Blast_Score=207, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003610
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF02839 CBM_5_12 [H]

EC number: =3.2.1.14; =3.2.1.17 [H]

Molecular weight: Translated: 57639; Mature: 57639

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSRKITAIILSVLVASGATVSVFTGKNVVQAKELEINSVQSKTATNIGVGQEYWGSTVF
CCCCHHHHHHHHHHHHCCCEEEEEECCCEEEEHHCCHHHHHHHHHCCCCCCHHHHCCCCH
APYVDACSYPVFSLTEYAKATGTKHFILGFVVDKNGQPSWGTYYDMEEGPTDYQGGGLLK
HHHHHHHCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCEEEECCCCCCCCCCCCHHH
EIKALRQMGGDVMVSFGGEANTPLAASIKDVNKLKDAYKKIIKDYGLTHVDFDIEGAWTA
HHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
DAASIERRSQAIALLQKELKAENHPLEIWYTLPVLPTGMTNGVDVVKNAVKNGVQLSGVN
CHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCEEECCCE
VMTMCFGQKAPDGTMGDLVIQSAKSVHKQLKDIYTQANIQKTDKEIWKMIGVTPMNGLDY
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCC
SGSILDQNGASKVASFGKTVGMGLIGMWSVNRDYQNPKGSTNYVSITDSSILQEKFEFGK
CCCEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEECCHHHHHHHHHHHH
ILSKYDSNDGTEIIEKPGTTDPEDPGDGEDGGETGEIAAYNPNTEYWAGDKVSYKGKVYQ
HHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEECCCEEEE
AKWWTKGFAPDTAVQNSWDTPWELIGDSDNDIEIPEKPGTTDPVDPEDGKDGEQTGEIAA
EEECCCCCCCCCCCCCCCCCCHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
YTPNKEYYEGDKVTYKGKVYQAKWWTKGFAPDTVVKNTWDTPWELIG
ECCCCCCCCCCEEEEECEEEEEECCCCCCCCCCHHCCCCCCCHHHCC
>Mature Secondary Structure
MKSRKITAIILSVLVASGATVSVFTGKNVVQAKELEINSVQSKTATNIGVGQEYWGSTVF
CCCCHHHHHHHHHHHHCCCEEEEEECCCEEEEHHCCHHHHHHHHHCCCCCCHHHHCCCCH
APYVDACSYPVFSLTEYAKATGTKHFILGFVVDKNGQPSWGTYYDMEEGPTDYQGGGLLK
HHHHHHHCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCEEEECCCCCCCCCCCCHHH
EIKALRQMGGDVMVSFGGEANTPLAASIKDVNKLKDAYKKIIKDYGLTHVDFDIEGAWTA
HHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
DAASIERRSQAIALLQKELKAENHPLEIWYTLPVLPTGMTNGVDVVKNAVKNGVQLSGVN
CHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCEEECCCE
VMTMCFGQKAPDGTMGDLVIQSAKSVHKQLKDIYTQANIQKTDKEIWKMIGVTPMNGLDY
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCC
SGSILDQNGASKVASFGKTVGMGLIGMWSVNRDYQNPKGSTNYVSITDSSILQEKFEFGK
CCCEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEECCHHHHHHHHHHHH
ILSKYDSNDGTEIIEKPGTTDPEDPGDGEDGGETGEIAAYNPNTEYWAGDKVSYKGKVYQ
HHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEECCCEEEE
AKWWTKGFAPDTAVQNSWDTPWELIGDSDNDIEIPEKPGTTDPVDPEDGKDGEQTGEIAA
EEECCCCCCCCCCCCCCCCCCHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
YTPNKEYYEGDKVTYKGKVYQAKWWTKGFAPDTVVKNTWDTPWELIG
ECCCCCCCCCCEEEEECEEEEEECCCCCCCCCCHHCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 2661540; 10419961; 10760150 [H]