BacMap

Definition	Clostridium botulinum A2 str. Kyoto chromosome, complete genome.
Accession	NC_012563
Length	4,155,278

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The map label for this gene is yvgN [H]

Identifier: 226948259

GI number: 226948259

Start: 1176576

End: 1177421

Strand: Direct

Name: yvgN [H]

Synonym: CLM_1138

Alternate gene names: 226948259

Gene position: 1176576-1177421 (Clockwise)

Preceding gene: 226948258

Following gene: 226948260

Centisome position: 28.32

GC content: 32.15

Gene sequence:

>846_bases
ATGCAAGATATAAAGAGTTATACAATTTTAAATAACGGAGTAAAGATGCCTTGGCTTGGATTTGGTACTTATAAGGTGGA
AAATGGGGACACGGTTATTCAATCAGTAAAGGAAGCTCTTAAAATTGGATATGGTCATATAGATACTGCTTCTTATTATG
GAAACGAAGAAGGAGTTGGTACTGCAATAAAAGAAAGCAGAATTCCTAGAGAAGATATATTTTTAGTAAGCAAAGTTTGG
AATTCAGAGCAGGGCTATGATAAAACTTTAAAATCCTTTGAAGATTCTATAAAAAAACTTGGCACAGATTATCTTGATTT
ATATTTAATTCATTGGCCTCAACCTTTAAGCAAAGAAACGTGGAAGGCCTTAGAAAAACTTTATAAAGAAGGACGTGTAA
AAGCTATTGGAGTAAGCAATTTTTTAGTTGATCACTTAAAGTGGTTATTAGAAGATGCCGAAATCATGCCAATGGTAAAT
CAGGTGGAATTTCATCCTCAACTTATTCAAAAGGACTTAATAGAGTTCTGCAGCAAAAATAATATACAATTAGAAGCATG
GTCTCCATTAATGAGAGGTAAAGTATTCGAAATCCAACTTTTACAGGATATTGCTCGAAAATACGGAAAAACAATATCTC
AAATCGTTTTAAGATGGGATCTACAAATGGGTGTGGTTACTATTCCTAAATCAATAAACCCATCAAGAATAAAAGAAAAT
GCAGATATATTCGACTTTGAAATAAGTAAAGAAGATATGGATAAAATTCAGCAACTAGATAAAGGTTTAAGGGTGGGCTC
AGACCCTAATAAAGTTTATCCATGTCAAAGTATATCTAATAAATAG

Upstream 100 bases:

>100_bases
TAGAGTTGTAGTGTACAAACTAACTTATACAAGCCATTAAGTTGTGTTAAGTAATTATGAAAAGATAGCTTTAAAAATCA
TACTTATAAGGAGTGATTTA

Downstream 100 bases:

>100_bases
TATTATTAAGATTAATGTTCCTATTTTATATAGTGAACAGAAGGTATTTTATAAAAAGGATTTTAAATAATGACAAGTAT
TCATATAATGGGGGAATGAA

Product: aldo/keto reductase family oxidoreductase

Products: NA

Alternate protein names: GR; Methylglyoxal reductase [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MQDIKSYTILNNGVKMPWLGFGTYKVENGDTVIQSVKEALKIGYGHIDTASYYGNEEGVGTAIKESRIPREDIFLVSKVW
NSEQGYDKTLKSFEDSIKKLGTDYLDLYLIHWPQPLSKETWKALEKLYKEGRVKAIGVSNFLVDHLKWLLEDAEIMPMVN
QVEFHPQLIQKDLIEFCSKNNIQLEAWSPLMRGKVFEIQLLQDIARKYGKTISQIVLRWDLQMGVVTIPKSINPSRIKEN
ADIFDFEISKEDMDKIQQLDKGLRVGSDPNKVYPCQSISNK

Sequences:

>Translated_281_residues
MQDIKSYTILNNGVKMPWLGFGTYKVENGDTVIQSVKEALKIGYGHIDTASYYGNEEGVGTAIKESRIPREDIFLVSKVW
NSEQGYDKTLKSFEDSIKKLGTDYLDLYLIHWPQPLSKETWKALEKLYKEGRVKAIGVSNFLVDHLKWLLEDAEIMPMVN
QVEFHPQLIQKDLIEFCSKNNIQLEAWSPLMRGKVFEIQLLQDIARKYGKTISQIVLRWDLQMGVVTIPKSINPSRIKEN
ADIFDFEISKEDMDKIQQLDKGLRVGSDPNKVYPCQSISNK
>Mature_281_residues
MQDIKSYTILNNGVKMPWLGFGTYKVENGDTVIQSVKEALKIGYGHIDTASYYGNEEGVGTAIKESRIPREDIFLVSKVW
NSEQGYDKTLKSFEDSIKKLGTDYLDLYLIHWPQPLSKETWKALEKLYKEGRVKAIGVSNFLVDHLKWLLEDAEIMPMVN
QVEFHPQLIQKDLIEFCSKNNIQLEAWSPLMRGKVFEIQLLQDIARKYGKTISQIVLRWDLQMGVVTIPKSINPSRIKEN
ADIFDFEISKEDMDKIQQLDKGLRVGSDPNKVYPCQSISNK

Specific function: Reduces glyoxal and methylglyoxal. Is not involved in the vitamin B6 biosynthesis [H]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI24497577, Length=295, Percent_Identity=38.6440677966102, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI5174391, Length=295, Percent_Identity=38.6440677966102, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI223468663, Length=299, Percent_Identity=37.7926421404682, Blast_Score=185, Evalue=3e-47,
Organism=Homo sapiens, GI93277124, Length=314, Percent_Identity=35.6687898089172, Blast_Score=183, Evalue=1e-46,
Organism=Homo sapiens, GI310109922, Length=274, Percent_Identity=37.5912408759124, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI5174695, Length=296, Percent_Identity=35.8108108108108, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI4502049, Length=301, Percent_Identity=38.8704318936877, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI45446745, Length=295, Percent_Identity=35.5932203389831, Blast_Score=175, Evalue=3e-44,
Organism=Homo sapiens, GI4503285, Length=295, Percent_Identity=35.5932203389831, Blast_Score=175, Evalue=3e-44,
Organism=Homo sapiens, GI24497583, Length=310, Percent_Identity=36.1290322580645, Blast_Score=175, Evalue=5e-44,
Organism=Homo sapiens, GI5453543, Length=295, Percent_Identity=35.2542372881356, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI310109920, Length=295, Percent_Identity=35.2542372881356, Blast_Score=173, Evalue=1e-43,
Organism=Homo sapiens, GI24497585, Length=295, Percent_Identity=37.2881355932203, Blast_Score=172, Evalue=2e-43,
Organism=Homo sapiens, GI291291012, Length=276, Percent_Identity=37.3188405797101, Blast_Score=167, Evalue=9e-42,
Organism=Homo sapiens, GI300116273, Length=275, Percent_Identity=34.9090909090909, Blast_Score=154, Evalue=6e-38,
Organism=Homo sapiens, GI300116271, Length=273, Percent_Identity=34.4322344322344, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI310109924, Length=154, Percent_Identity=35.0649350649351, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI207028673, Length=123, Percent_Identity=41.4634146341463, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI310109926, Length=123, Percent_Identity=40.650406504065, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI87082198, Length=267, Percent_Identity=44.5692883895131, Blast_Score=248, Evalue=4e-67,
Organism=Escherichia coli, GI1786400, Length=267, Percent_Identity=33.3333333333333, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1788081, Length=258, Percent_Identity=31.7829457364341, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1788070, Length=317, Percent_Identity=24.2902208201893, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI48994888, Length=226, Percent_Identity=29.2035398230088, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87081735, Length=199, Percent_Identity=27.6381909547739, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787674, Length=251, Percent_Identity=24.7011952191235, Blast_Score=71, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI71998625, Length=285, Percent_Identity=41.4035087719298, Blast_Score=217, Evalue=7e-57,
Organism=Caenorhabditis elegans, GI17561298, Length=272, Percent_Identity=35.6617647058824, Blast_Score=186, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17564128, Length=296, Percent_Identity=35.1351351351351, Blast_Score=181, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17538386, Length=272, Percent_Identity=37.5, Blast_Score=181, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17552492, Length=277, Percent_Identity=35.0180505415162, Blast_Score=179, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17537077, Length=292, Percent_Identity=34.9315068493151, Blast_Score=177, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI17537075, Length=297, Percent_Identity=34.6801346801347, Blast_Score=174, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI17550248, Length=300, Percent_Identity=35.3333333333333, Blast_Score=171, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17566692, Length=290, Percent_Identity=35.1724137931034, Blast_Score=164, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17562292, Length=275, Percent_Identity=33.8181818181818, Blast_Score=157, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17537079, Length=303, Percent_Identity=29.3729372937294, Blast_Score=157, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI17561300, Length=270, Percent_Identity=32.2222222222222, Blast_Score=138, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17550246, Length=84, Percent_Identity=48.8095238095238, Blast_Score=74, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6320576, Length=286, Percent_Identity=39.1608391608392, Blast_Score=199, Evalue=6e-52,
Organism=Saccharomyces cerevisiae, GI6324694, Length=294, Percent_Identity=37.4149659863946, Blast_Score=191, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6319625, Length=294, Percent_Identity=37.0748299319728, Blast_Score=171, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6322556, Length=269, Percent_Identity=34.2007434944238, Blast_Score=162, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6320079, Length=283, Percent_Identity=36.7491166077738, Blast_Score=162, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6321896, Length=317, Percent_Identity=32.4921135646688, Blast_Score=162, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24663317, Length=291, Percent_Identity=42.2680412371134, Blast_Score=204, Evalue=7e-53,
Organism=Drosophila melanogaster, GI24657054, Length=310, Percent_Identity=37.0967741935484, Blast_Score=198, Evalue=3e-51,
Organism=Drosophila melanogaster, GI24644950, Length=302, Percent_Identity=35.4304635761589, Blast_Score=192, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24662789, Length=294, Percent_Identity=37.4149659863946, Blast_Score=187, Evalue=9e-48,
Organism=Drosophila melanogaster, GI221378297, Length=322, Percent_Identity=33.5403726708075, Blast_Score=185, Evalue=3e-47,
Organism=Drosophila melanogaster, GI21356425, Length=293, Percent_Identity=36.1774744027304, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI20129731, Length=307, Percent_Identity=35.1791530944625, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI45553081, Length=289, Percent_Identity=35.9861591695502, Blast_Score=182, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24662781, Length=275, Percent_Identity=39.6363636363636, Blast_Score=158, Evalue=4e-39,
Organism=Drosophila melanogaster, GI281366140, Length=257, Percent_Identity=35.0194552529183, Blast_Score=157, Evalue=6e-39,
Organism=Drosophila melanogaster, GI24662785, Length=273, Percent_Identity=39.1941391941392, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24646155, Length=135, Percent_Identity=40, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: =1.1.1.283 [H]

Molecular weight: Translated: 32325; Mature: 32325

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS00062 ALDOKETO_REDUCTASE_2 ; PS00063 ALDOKETO_REDUCTASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDIKSYTILNNGVKMPWLGFGTYKVENGDTVIQSVKEALKIGYGHIDTASYYGNEEGVG
CCCCCCEEEECCCCCCCEECCCEEEECCCHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCC
TAIKESRIPREDIFLVSKVWNSEQGYDKTLKSFEDSIKKLGTDYLDLYLIHWPQPLSKET
HHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHEEEEEEECCCCCCHHH
WKALEKLYKEGRVKAIGVSNFLVDHLKWLLEDAEIMPMVNQVEFHPQLIQKDLIEFCSKN
HHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCHHHHHHHHHHHHCHHHHHHHHHHHHCCC
NIQLEAWSPLMRGKVFEIQLLQDIARKYGKTISQIVLRWDLQMGVVTIPKSINPSRIKEN
CEEEEECCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHCC
ADIFDFEISKEDMDKIQQLDKGLRVGSDPNKVYPCQSISNK
CCEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQDIKSYTILNNGVKMPWLGFGTYKVENGDTVIQSVKEALKIGYGHIDTASYYGNEEGVG
CCCCCCEEEECCCCCCCEECCCEEEECCCHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCC
TAIKESRIPREDIFLVSKVWNSEQGYDKTLKSFEDSIKKLGTDYLDLYLIHWPQPLSKET
HHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHEEEEEEECCCCCCHHH
WKALEKLYKEGRVKAIGVSNFLVDHLKWLLEDAEIMPMVNQVEFHPQLIQKDLIEFCSKN
HHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCHHHHHHHHHHHHCHHHHHHHHHHHHCCC
NIQLEAWSPLMRGKVFEIQLLQDIARKYGKTISQIVLRWDLQMGVVTIPKSINPSRIKEN
CEEEEECCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHCC
ADIFDFEISKEDMDKIQQLDKGLRVGSDPNKVYPCQSISNK
CCEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]