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The map label for this gene is cobM [H]

Identifier: 226948221

GI number: 226948221

Start: 1139071

End: 1139829

Strand: Direct

Name: cobM [H]

Synonym: CLM_1098

Alternate gene names: 226948221

Gene position: 1139071-1139829 (Clockwise)

Preceding gene: 226948220

Following gene: 226948222

Centisome position: 27.41

GC content: 33.07

Gene sequence:

>759_bases
ATGGAAAGTAAGGTTTATTTTATAGGAGCAGGTCCTGGGAATCCGGATTTAATAACAGTAAAGGGAAGAGATATTTTAAC
TAAAGCAGATGTAGTTATATATGCAGGTTCCTTAGTAAGCAAAGAACATTTAGATTATTGCAAGGAAGGTGTAGAGGTTT
ATAATTCAGCTTCCATGACGTTAAAAGAAGTTATAAACGTTATACAAAAAGCCCATAATGAAAATAAATCTATAGTAAGA
CTTCATACGGGAGATCCATCTATATATGGAGCTATAAAGGAGCAAATGGATGAATTAGATAAGCTAAATATATGCTATGA
GGTTGTACCAGGAGTAAGTTCTTTTTCAGCAGCTGCAGCATCCATTAAGAAAGAATTTACACTACCAGGGGTTAGTCAAA
CCCTAATACTTACAAGGGTAGAGGGAAGAACACCAGTACCAGAAAAAGAAGATTTGGAAGTTTTAGCAAGTAGAGGAGCA
TCCATGGCACTATTTTTATCAGTAGGTATGATTGATAAAGTAGCTTTAAAATTAAGAAAAGGATATGGAAGAAATGTTCC
AATAGCAGTAATTCAAAGAGCTACTTGGCAGGATGAAAAAGTAGTTATAGGAACTTTAGATGATATAGCTAAAAAGGTTA
AAGATGCTAATATAACAAAAACAGCTCAAATATTAGTAGGGGATTTTATAGATTGTAAATATGACAAAAGCCTACTATAT
GATGAGAAATTTACCCATGAATTTAGAAAAGGAATCTAG

Upstream 100 bases:

>100_bases
GGTTATCCAGGGACGTATCCGCTCTTTATTCCTACTTTGAATAAAATGGGAGTATTAGAGCGGGTAGTCATCGGATAAAT
TTGAAGATTGGAGATTTAAA

Downstream 100 bases:

>100_bases
GATAATGAAAATATCTATAATTAATGTAACCAAGATGGGTAATGATATAGCCTATAAAATTAAAGAACATATGGATGCAG
ATTTATATTCAAAATATGTT

Product: precorrin-4 C(11)-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MESKVYFIGAGPGNPDLITVKGRDILTKADVVIYAGSLVSKEHLDYCKEGVEVYNSASMTLKEVINVIQKAHNENKSIVR
LHTGDPSIYGAIKEQMDELDKLNICYEVVPGVSSFSAAAASIKKEFTLPGVSQTLILTRVEGRTPVPEKEDLEVLASRGA
SMALFLSVGMIDKVALKLRKGYGRNVPIAVIQRATWQDEKVVIGTLDDIAKKVKDANITKTAQILVGDFIDCKYDKSLLY
DEKFTHEFRKGI

Sequences:

>Translated_252_residues
MESKVYFIGAGPGNPDLITVKGRDILTKADVVIYAGSLVSKEHLDYCKEGVEVYNSASMTLKEVINVIQKAHNENKSIVR
LHTGDPSIYGAIKEQMDELDKLNICYEVVPGVSSFSAAAASIKKEFTLPGVSQTLILTRVEGRTPVPEKEDLEVLASRGA
SMALFLSVGMIDKVALKLRKGYGRNVPIAVIQRATWQDEKVVIGTLDDIAKKVKDANITKTAQILVGDFIDCKYDKSLLY
DEKFTHEFRKGI
>Mature_252_residues
MESKVYFIGAGPGNPDLITVKGRDILTKADVVIYAGSLVSKEHLDYCKEGVEVYNSASMTLKEVINVIQKAHNENKSIVR
LHTGDPSIYGAIKEQMDELDKLNICYEVVPGVSSFSAAAASIKKEFTLPGVSQTLILTRVEGRTPVPEKEDLEVLASRGA
SMALFLSVGMIDKVALKLRKGYGRNVPIAVIQRATWQDEKVVIGTLDDIAKKVKDANITKTAQILVGDFIDCKYDKSLLY
DEKFTHEFRKGI

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=221, Percent_Identity=26.6968325791855, Blast_Score=80, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 27753; Mature: 27753

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00839 SUMT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESKVYFIGAGPGNPDLITVKGRDILTKADVVIYAGSLVSKEHLDYCKEGVEVYNSASMT
CCCEEEEEECCCCCCCEEEECCCCEEECCCEEEEECCHHCHHHHHHHHHHHHHHCCCHHH
LKEVINVIQKAHNENKSIVRLHTGDPSIYGAIKEQMDELDKLNICYEVVPGVSSFSAAAA
HHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
SIKKEFTLPGVSQTLILTRVEGRTPVPEKEDLEVLASRGASMALFLSVGMIDKVALKLRK
HHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHH
GYGRNVPIAVIQRATWQDEKVVIGTLDDIAKKVKDANITKTAQILVGDFIDCKYDKSLLY
CCCCCCCHHEEECCCCCCCEEEEECHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHC
DEKFTHEFRKGI
CHHHHHHHHHCC
>Mature Secondary Structure
MESKVYFIGAGPGNPDLITVKGRDILTKADVVIYAGSLVSKEHLDYCKEGVEVYNSASMT
CCCEEEEEECCCCCCCEEEECCCCEEECCCEEEEECCHHCHHHHHHHHHHHHHHCCCHHH
LKEVINVIQKAHNENKSIVRLHTGDPSIYGAIKEQMDELDKLNICYEVVPGVSSFSAAAA
HHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
SIKKEFTLPGVSQTLILTRVEGRTPVPEKEDLEVLASRGASMALFLSVGMIDKVALKLRK
HHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHH
GYGRNVPIAVIQRATWQDEKVVIGTLDDIAKKVKDANITKTAQILVGDFIDCKYDKSLLY
CCCCCCCHHEEECCCCCCCEEEEECHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHC
DEKFTHEFRKGI
CHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]