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The map label for this gene is cbiE [H]

Identifier: 226948210

GI number: 226948210

Start: 1132787

End: 1133458

Strand: Direct

Name: cbiE [H]

Synonym: CLM_1087

Alternate gene names: 226948210

Gene position: 1132787-1133458 (Clockwise)

Preceding gene: 226948209

Following gene: 226948211

Centisome position: 27.26

GC content: 27.98

Gene sequence:

>672_bases
ATGAATTCTACACAGGAGAACCTACAATTTAGAAAGGTTTATATAGTAGGTTTAGGGCCAGGGCATAAAAATTATATGAT
TCATGGTGCTATAAATAATTTAGAAAATGTAGATGTAATAATAGGATTTAAAAGAGCAATAGAAAGCTTAGATTTTATAA
AAAATAATAAGAAAATAGTAAATAAATTAAGTGAGATATTAGATTACATAAGAGAAAATAAGGATAAAGATATATCTATA
GTGGCCTCTGGAGATCCTTGTTTTTATGGGGTAAGCAATTATATTAAAAATAACTATGAAGATAAAATAGAGGTAATTCC
GGGTATAAGCTCTTATCAATACATGATGGCAAAAATTAATGAAAGCTGGCAAAATAGTTTTTTAGGAAGTCTACATGGTA
GAGAAGAAGAATTTATAGAAAAAGTCAAAAGCTATGAAAAATCTATTTGGCTTACAGATAAAAAGAATAGTCCAGATAAA
TTATGTAAAAAACTAATAGAAAACAACATAAAAGCAGAGGTGACAGTAGGAGAAAATTTATCTTATAATGATGAAAGGAT
AATTAAAGGTAACCCAGAGCAATTAGAAAATATGAATTTCGGAAATCTTACGGTAGTGTATATAAACAGAGTTTTTGACT
TCAGAGGGAGTTTTACTCCCGTTAAATCTTAG

Upstream 100 bases:

>100_bases
ATGATAACAGTAATTAGAAGTTTAAAAATCTAGAGATATTTATAGAAAATAACTCAATACTGTCTATCACACTAATATCA
CCTATAAGGGGGGAGCTTTG

Downstream 100 bases:

>100_bases
GAAATGGTTAGCTAGGGATGTATCCATTTTTTACTGCCACTTTGAAGAAGATGGAAGTAAAGAGCTGGTAGTCATGGGAT
AAAAGCTAAGTTCTAAATGA

Product: precorrin-6y C5,15-methyltransferase subunit CbiE

Products: S-adenosyl-L-homocysteine; Precorrin 8X; CO2

Alternate protein names: Cobalt-precorrin-6 methyltransferase; Cobalt-precorrin-6Y methylase [H]

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MNSTQENLQFRKVYIVGLGPGHKNYMIHGAINNLENVDVIIGFKRAIESLDFIKNNKKIVNKLSEILDYIRENKDKDISI
VASGDPCFYGVSNYIKNNYEDKIEVIPGISSYQYMMAKINESWQNSFLGSLHGREEEFIEKVKSYEKSIWLTDKKNSPDK
LCKKLIENNIKAEVTVGENLSYNDERIIKGNPEQLENMNFGNLTVVYINRVFDFRGSFTPVKS

Sequences:

>Translated_223_residues
MNSTQENLQFRKVYIVGLGPGHKNYMIHGAINNLENVDVIIGFKRAIESLDFIKNNKKIVNKLSEILDYIRENKDKDISI
VASGDPCFYGVSNYIKNNYEDKIEVIPGISSYQYMMAKINESWQNSFLGSLHGREEEFIEKVKSYEKSIWLTDKKNSPDK
LCKKLIENNIKAEVTVGENLSYNDERIIKGNPEQLENMNFGNLTVVYINRVFDFRGSFTPVKS
>Mature_223_residues
MNSTQENLQFRKVYIVGLGPGHKNYMIHGAINNLENVDVIIGFKRAIESLDFIKNNKKIVNKLSEILDYIRENKDKDISI
VASGDPCFYGVSNYIKNNYEDKIEVIPGISSYQYMMAKINESWQNSFLGSLHGREEEFIEKVKSYEKSIWLTDKKNSPDK
LCKKLIENNIKAEVTVGENLSYNDERIIKGNPEQLENMNFGNLTVVYINRVFDFRGSFTPVKS

Specific function: Probably catalyzes the methylation of C-5 in cobalt- precorrin-6Y to form cobalt-precorrin-7W-a [H]

COG id: COG2241

COG function: function code H; Precorrin-6B methylase 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR012818 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.132; 1.-.-.-

Molecular weight: Translated: 25695; Mature: 25695

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSTQENLQFRKVYIVGLGPGHKNYMIHGAINNLENVDVIIGFKRAIESLDFIKNNKKIV
CCCCHHCCEEEEEEEEEECCCCCCEEEEEECCCCCCEEEEEEHHHHHHHHHHHHCCHHHH
NKLSEILDYIRENKDKDISIVASGDPCFYGVSNYIKNNYEDKIEVIPGISSYQYMMAKIN
HHHHHHHHHHHCCCCCCEEEEECCCCCEECCHHHHCCCCCCEEEECCCCCHHHHHHHHHH
ESWQNSFLGSLHGREEEFIEKVKSYEKSIWLTDKKNSPDKLCKKLIENNIKAEVTVGENL
HHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCC
SYNDERIIKGNPEQLENMNFGNLTVVYINRVFDFRGSFTPVKS
CCCCCEEECCCHHHHHCCCCCCEEEEEEEEHHHCCCCCCCCCC
>Mature Secondary Structure
MNSTQENLQFRKVYIVGLGPGHKNYMIHGAINNLENVDVIIGFKRAIESLDFIKNNKKIV
CCCCHHCCEEEEEEEEEECCCCCCEEEEEECCCCCCEEEEEEHHHHHHHHHHHHCCHHHH
NKLSEILDYIRENKDKDISIVASGDPCFYGVSNYIKNNYEDKIEVIPGISSYQYMMAKIN
HHHHHHHHHHHCCCCCCEEEEECCCCCEECCHHHHCCCCCCEEEECCCCCHHHHHHHHHH
ESWQNSFLGSLHGREEEFIEKVKSYEKSIWLTDKKNSPDKLCKKLIENNIKAEVTVGENL
HHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCC
SYNDERIIKGNPEQLENMNFGNLTVVYINRVFDFRGSFTPVKS
CCCCCEEECCCHHHHHCCCCCCEEEEEEEEHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 6Y

Specific reaction: 2 S-adenosyl-L-methionine + precorrin-6Y = 2 S-adenosyl-L-homocysteine + precorrin-8X + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]