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The map label for this gene is hemB [H]

Identifier: 226948196

GI number: 226948196

Start: 1120852

End: 1121826

Strand: Direct

Name: hemB [H]

Synonym: CLM_1071

Alternate gene names: 226948196

Gene position: 1120852-1121826 (Clockwise)

Preceding gene: 226948195

Following gene: 226948197

Centisome position: 26.97

GC content: 32.82

Gene sequence:

>975_bases
ATGTTTAGAAGACACAGAAGATTAAGAGAAAATGAAGCAATAAGAGCTATGGTAAGAGAAACAGTGATTAGAGAAGAGGA
TTTTATATATCCAATGTTTGTGGTAGAAGGAGAAAATATTAAAGAAGAAATATCTTCACTACCAGGAAATTATCATTATT
CTATAGATAGATTAGAAGAATTAGTAGAGGAAGTAAAAAAATCAGGAATAAAAGGTATAATGATATTTGGAGTACCAGAT
CATAAAGATGAATGTGGTTCCGCAGCCTTTGATGATAATGGAATAGTTCAAAAGGGTATAAGAAGATTAAGAGAATTAAT
GCCAGAACTATATATAGTTGCAGATGTATGTATGTGCCAATATACAAGTCATGGACATTGCGGAATAATTCATGGTCATA
ATGTAGATAATGATGAATCATTGAAATATTTAGCAAAAATATCTTTATCCTATGCAAAAGCTGGAGCAGATATGATAGCC
CCTTCAGATATGATGGATGGAAGAGTTTATGCTATAAGAAAAATATTAGATGAAAATAATTTTAAACATGTAGGCATAAT
GGCTTATAGTGCAAAATATTGTTCAGCTTTCTATGGCCCTTTCAGAGAAGCAGCTAATTCAGCACCACAATTTGGAGACA
GAAAAACTTATCAAATGGATCCAGCAAACGGAAGAGAAGCAATGCTTGAAATAGAAGACGATATAAATGAAGGTGCAGAT
ATAGTAATGGTAAAACCAGCTCTGCCATACTTAGATGTTATAAGAATGGCAAAGGATAGATTTGAATTCCCGTTAGCAGC
TTACAATGTAAGTGGAGAATATGCTATGGTAAAAGCAGCAGCTAAACAAGGATTAATAGACGAAAGAAGAGTAGCCCTAG
AAATGCTAACATCAATAAAAAGAGCAGGAGCAGATATAATAATAACTTATTATGCATTTGAAGCAAGTGCTTGGCTAAAA
GAAGACAGAAAATAG

Upstream 100 bases:

>100_bases
AATAATAATTAAGCTATTAAACTATAAAATCTTTGTAGTTTATATATAAATAAATTTAATTTGATTAGCTTAGGCTAGAT
ATTAAAAGGAGACGAGAATT

Downstream 100 bases:

>100_bases
TACATAAAATGAATTAGTTTAAAGTTGAGGGTGGAATTTTTTCATTTTATGAAAAAATTCCACTCTGTACTCTGTACTCT
GTACTCTGTACTCTGTACTC

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MFRRHRRLRENEAIRAMVRETVIREEDFIYPMFVVEGENIKEEISSLPGNYHYSIDRLEELVEEVKKSGIKGIMIFGVPD
HKDECGSAAFDDNGIVQKGIRRLRELMPELYIVADVCMCQYTSHGHCGIIHGHNVDNDESLKYLAKISLSYAKAGADMIA
PSDMMDGRVYAIRKILDENNFKHVGIMAYSAKYCSAFYGPFREAANSAPQFGDRKTYQMDPANGREAMLEIEDDINEGAD
IVMVKPALPYLDVIRMAKDRFEFPLAAYNVSGEYAMVKAAAKQGLIDERRVALEMLTSIKRAGADIIITYYAFEASAWLK
EDRK

Sequences:

>Translated_324_residues
MFRRHRRLRENEAIRAMVRETVIREEDFIYPMFVVEGENIKEEISSLPGNYHYSIDRLEELVEEVKKSGIKGIMIFGVPD
HKDECGSAAFDDNGIVQKGIRRLRELMPELYIVADVCMCQYTSHGHCGIIHGHNVDNDESLKYLAKISLSYAKAGADMIA
PSDMMDGRVYAIRKILDENNFKHVGIMAYSAKYCSAFYGPFREAANSAPQFGDRKTYQMDPANGREAMLEIEDDINEGAD
IVMVKPALPYLDVIRMAKDRFEFPLAAYNVSGEYAMVKAAAKQGLIDERRVALEMLTSIKRAGADIIITYYAFEASAWLK
EDRK
>Mature_324_residues
MFRRHRRLRENEAIRAMVRETVIREEDFIYPMFVVEGENIKEEISSLPGNYHYSIDRLEELVEEVKKSGIKGIMIFGVPD
HKDECGSAAFDDNGIVQKGIRRLRELMPELYIVADVCMCQYTSHGHCGIIHGHNVDNDESLKYLAKISLSYAKAGADMIA
PSDMMDGRVYAIRKILDENNFKHVGIMAYSAKYCSAFYGPFREAANSAPQFGDRKTYQMDPANGREAMLEIEDDINEGAD
IVMVKPALPYLDVIRMAKDRFEFPLAAYNVSGEYAMVKAAAKQGLIDERRVALEMLTSIKRAGADIIITYYAFEASAWLK
EDRK

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=307, Percent_Identity=45.928338762215, Blast_Score=288, Evalue=6e-78,
Organism=Escherichia coli, GI87081728, Length=314, Percent_Identity=48.4076433121019, Blast_Score=301, Evalue=5e-83,
Organism=Saccharomyces cerevisiae, GI6321398, Length=324, Percent_Identity=42.2839506172839, Blast_Score=259, Evalue=3e-70,
Organism=Drosophila melanogaster, GI21358291, Length=293, Percent_Identity=45.7337883959044, Blast_Score=275, Evalue=3e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 36798; Mature: 36798

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRRHRRLRENEAIRAMVRETVIREEDFIYPMFVVEGENIKEEISSLPGNYHYSIDRLEE
CCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHCCCCCEECHHHHHH
LVEEVKKSGIKGIMIFGVPDHKDECGSAAFDDNGIVQKGIRRLRELMPELYIVADVCMCQ
HHHHHHHCCCCEEEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YTSHGHCGIIHGHNVDNDESLKYLAKISLSYAKAGADMIAPSDMMDGRVYAIRKILDENN
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCHHHHHHHHHCCCC
FKHVGIMAYSAKYCSAFYGPFREAANSAPQFGDRKTYQMDPANGREAMLEIEDDINEGAD
CCEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCEEEEEHHCCCCCCC
IVMVKPALPYLDVIRMAKDRFEFPLAAYNVSGEYAMVKAAAKQGLIDERRVALEMLTSIK
EEEECCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHH
RAGADIIITYYAFEASAWLKEDRK
HCCCCEEEEEEEEHHHHHHHCCCC
>Mature Secondary Structure
MFRRHRRLRENEAIRAMVRETVIREEDFIYPMFVVEGENIKEEISSLPGNYHYSIDRLEE
CCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHCCCCCEECHHHHHH
LVEEVKKSGIKGIMIFGVPDHKDECGSAAFDDNGIVQKGIRRLRELMPELYIVADVCMCQ
HHHHHHHCCCCEEEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YTSHGHCGIIHGHNVDNDESLKYLAKISLSYAKAGADMIAPSDMMDGRVYAIRKILDENN
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCHHHHHHHHHCCCC
FKHVGIMAYSAKYCSAFYGPFREAANSAPQFGDRKTYQMDPANGREAMLEIEDDINEGAD
CCEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCEEEEEHHCCCCCCC
IVMVKPALPYLDVIRMAKDRFEFPLAAYNVSGEYAMVKAAAKQGLIDERRVALEMLTSIK
EEEECCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHH
RAGADIIITYYAFEASAWLKEDRK
HCCCCEEEEEEEEHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1672867; 9384377 [H]