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The map label for this gene is hemD [H]

Identifier: 226948194

GI number: 226948194

Start: 1118803

End: 1120263

Strand: Direct

Name: hemD [H]

Synonym: CLM_1069

Alternate gene names: 226948194

Gene position: 1118803-1120263 (Clockwise)

Preceding gene: 226948193

Following gene: 226948195

Centisome position: 26.92

GC content: 30.39

Gene sequence:

>1461_bases
ATGAGTAAAGTATATTTAATTGGTGCAGGGCCAGGTGATGAAGAATTAATAACTTTAAAAGCCATAAGAGCCCTAAAAAA
ATGTACTGCAGTTATGTATGATAGATTAGCAAATGGAGAATTGTTAAAATATTTAGCACCAAATTGTGAAATTTATTATT
GTGGAAAAGAACCGGGCTGTCATTATAAAAGCCAGGATGAAATAAATAAAATGCTAGTTAAATTAGCAAAAGAAGGTCAT
ATTGTAGGAAGAATAAAAGGTGGAGATCCCTATGTATTTGGAAGAGGGGGAGAAGAAGCCTTGGATATTTTAGAAGAAAA
TATAGAATTTGAGGTTATACCAGGTGTAACTTCTCCAGTATCTGTACTGAATTATGCAGGAATTCCTATAACCCATAGAG
GAATATCAAGAGGGTTTCATATTTTTACAGCCATGACTAAGGATAAATTAGATATAGATTGGAAGTCTGTAGTTAATATA
GGAGGAACCTTAGTATTTTTAATGGGGCTTGGAAGATTAGAATTAATAACTAAAGGTCTTATAGAAAATGGAATGGACAA
AGAAGGTAAAGTAGCAGTGGTTATGAAAGGAACTACCTCAAAGCAAAAAAAAGTTATAGGTAACTTAGAAAATATAGTAG
AAAAAGTAAAAGAAGCTAAACTAGAGTCACCAGTAATAATAGTAGTAGGAGAAGTAGTTAGTTTTTCAGATAAATTAAAT
TGGTATGAAAAAAAGCCTTTATTCGGAAGAAATATATGCATAACCCGTACAAAAGAGCAAGCTAAGGAGTTAAAAGTTCA
ACTTTTAGATTTAGGGGCTGAAGTCACAGAAATAAATTCTATAGAAATAAAAAATGCAGAGGAGAACTTAAAGCCTTATT
TAAATAGATTAAAAGAATATGATTATATAGCTTTGACTAGTGTAAATGCAGTTAAAATATTTTTTGATTATTTAATTAAA
GAAAATATAGATATTAGAAATATTAATGCTAAATTTGCAGCTATAGGACCAGCTACTAGTGAAGCAATAAAAGTGCGAGG
AATAATGCCAAGTATAAAATCAAAGCATTTTGTAGCTGAAAGCCTTTTTGAAGAAATGAAAAAGCATGTTCAAAGTGGGG
ATAAGGTACTTGTACCTCGCTCAAAAGATGCAAGGCCCTTTCTTGTAGATGCATTAAAAAAAGAAGGTTGCATTGTAGAT
GAAGTACATATATATGAAACTTTATGTGGACAATCTTCTCATACTGAAAGATTTGAAGATGCAGATACAGTATTATTTAC
AAGTCCATCAACTGTTAAAAATATGATTTCCATGGTGGGGATAGATAGTATAAAAGAAAAAAGTATTATAGCAATAGGTC
CTATTACAGCAGCAGAATTATATAAAAATAATATAGAATGTAGTATTTGTGATGAATATTCTATAAATGGCATAATAGAT
AAACTTTTAGACCTTAACTAG

Upstream 100 bases:

>100_bases
CTGATCTTAATAGAAGATGTGAGTATTATAAGTGGTATTAGTGGGAAATAGTAATTTTGAATAAAAAGGTATCCCACACT
TTTATGTTTAGGAGGAAAGT

Downstream 100 bases:

>100_bases
ATGCTGAGAATAATGGTAAATTAATCAACATATTATAAAAAATTAAATCCATCCATTAGGGATAAGAAAGCATATGGGTA
TAGATAAAGAGTACTATGTT

Product: uroporphyrinogen III synthase/methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Uroporphyrinogen-III synthase; UROS; Hydroxymethylbilane hydrolyase [cyclizing]; Uroporphyrinogen-III cosynthase [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MSKVYLIGAGPGDEELITLKAIRALKKCTAVMYDRLANGELLKYLAPNCEIYYCGKEPGCHYKSQDEINKMLVKLAKEGH
IVGRIKGGDPYVFGRGGEEALDILEENIEFEVIPGVTSPVSVLNYAGIPITHRGISRGFHIFTAMTKDKLDIDWKSVVNI
GGTLVFLMGLGRLELITKGLIENGMDKEGKVAVVMKGTTSKQKKVIGNLENIVEKVKEAKLESPVIIVVGEVVSFSDKLN
WYEKKPLFGRNICITRTKEQAKELKVQLLDLGAEVTEINSIEIKNAEENLKPYLNRLKEYDYIALTSVNAVKIFFDYLIK
ENIDIRNINAKFAAIGPATSEAIKVRGIMPSIKSKHFVAESLFEEMKKHVQSGDKVLVPRSKDARPFLVDALKKEGCIVD
EVHIYETLCGQSSHTERFEDADTVLFTSPSTVKNMISMVGIDSIKEKSIIAIGPITAAELYKNNIECSICDEYSINGIID
KLLDLN

Sequences:

>Translated_486_residues
MSKVYLIGAGPGDEELITLKAIRALKKCTAVMYDRLANGELLKYLAPNCEIYYCGKEPGCHYKSQDEINKMLVKLAKEGH
IVGRIKGGDPYVFGRGGEEALDILEENIEFEVIPGVTSPVSVLNYAGIPITHRGISRGFHIFTAMTKDKLDIDWKSVVNI
GGTLVFLMGLGRLELITKGLIENGMDKEGKVAVVMKGTTSKQKKVIGNLENIVEKVKEAKLESPVIIVVGEVVSFSDKLN
WYEKKPLFGRNICITRTKEQAKELKVQLLDLGAEVTEINSIEIKNAEENLKPYLNRLKEYDYIALTSVNAVKIFFDYLIK
ENIDIRNINAKFAAIGPATSEAIKVRGIMPSIKSKHFVAESLFEEMKKHVQSGDKVLVPRSKDARPFLVDALKKEGCIVD
EVHIYETLCGQSSHTERFEDADTVLFTSPSTVKNMISMVGIDSIKEKSIIAIGPITAAELYKNNIECSICDEYSINGIID
KLLDLN
>Mature_485_residues
SKVYLIGAGPGDEELITLKAIRALKKCTAVMYDRLANGELLKYLAPNCEIYYCGKEPGCHYKSQDEINKMLVKLAKEGHI
VGRIKGGDPYVFGRGGEEALDILEENIEFEVIPGVTSPVSVLNYAGIPITHRGISRGFHIFTAMTKDKLDIDWKSVVNIG
GTLVFLMGLGRLELITKGLIENGMDKEGKVAVVMKGTTSKQKKVIGNLENIVEKVKEAKLESPVIIVVGEVVSFSDKLNW
YEKKPLFGRNICITRTKEQAKELKVQLLDLGAEVTEINSIEIKNAEENLKPYLNRLKEYDYIALTSVNAVKIFFDYLIKE
NIDIRNINAKFAAIGPATSEAIKVRGIMPSIKSKHFVAESLFEEMKKHVQSGDKVLVPRSKDARPFLVDALKKEGCIVDE
VHIYETLCGQSSHTERFEDADTVLFTSPSTVKNMISMVGIDSIKEKSIIAIGPITAAELYKNNIECSICDEYSINGIIDK
LLDLN

Specific function: May catalyze sequential reactions to synthesize uroporphyrinogen III from hydroxymethylbilane (HMB) and then precorrin-2, which are intermediate compounds in both vitamin B12 and siroheme biosyntheses [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=240, Percent_Identity=38.75, Blast_Score=191, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6322922, Length=235, Percent_Identity=31.4893617021277, Blast_Score=112, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR003754
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF02602 HEM4; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =4.2.1.75 [H]

Molecular weight: Translated: 53985; Mature: 53853

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVYLIGAGPGDEELITLKAIRALKKCTAVMYDRLANGELLKYLAPNCEIYYCGKEPGC
CCEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEECCCCCC
HYKSQDEINKMLVKLAKEGHIVGRIKGGDPYVFGRGGEEALDILEENIEFEVIPGVTSPV
CCCCHHHHHHHHHHHHHCCCEEEEEECCCCEEECCCCHHHHHHHHCCCCEEEECCCCCHH
SVLNYAGIPITHRGISRGFHIFTAMTKDKLDIDWKSVVNIGGTLVFLMGLGRLELITKGL
HHHHCCCCCEEECCCCCCEEEEEEECCCCCCCCHHHHHHCCHHHHHHHCCCHHHHHHHHH
IENGMDKEGKVAVVMKGTTSKQKKVIGNLENIVEKVKEAKLESPVIIVVGEVVSFSDKLN
HHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHCC
WYEKKPLFGRNICITRTKEQAKELKVQLLDLGAEVTEINSIEIKNAEENLKPYLNRLKEY
CHHCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCEEECCEEEECCCHHHHHHHHHHHHCC
DYIALTSVNAVKIFFDYLIKENIDIRNINAKFAAIGPATSEAIKVRGIMPSIKSKHFVAE
CEEEEECCHHHHHHHHHHHHCCCCEEECCCEEEEECCCCCCCEEEEECCCCHHHHHHHHH
SLFEEMKKHVQSGDKVLVPRSKDARPFLVDALKKEGCIVDEVHIYETLCGQSSHTERFED
HHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHCCCEEEHHHHHHHHCCCCCCCCCCCC
ADTVLFTSPSTVKNMISMVGIDSIKEKSIIAIGPITAAELYKNNIECSICDEYSINGIID
CCEEEEECCHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHCCCCEEEECCCCHHHHHH
KLLDLN
HHHCCC
>Mature Secondary Structure 
SKVYLIGAGPGDEELITLKAIRALKKCTAVMYDRLANGELLKYLAPNCEIYYCGKEPGC
CEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEECCCCCC
HYKSQDEINKMLVKLAKEGHIVGRIKGGDPYVFGRGGEEALDILEENIEFEVIPGVTSPV
CCCCHHHHHHHHHHHHHCCCEEEEEECCCCEEECCCCHHHHHHHHCCCCEEEECCCCCHH
SVLNYAGIPITHRGISRGFHIFTAMTKDKLDIDWKSVVNIGGTLVFLMGLGRLELITKGL
HHHHCCCCCEEECCCCCCEEEEEEECCCCCCCCHHHHHHCCHHHHHHHCCCHHHHHHHHH
IENGMDKEGKVAVVMKGTTSKQKKVIGNLENIVEKVKEAKLESPVIIVVGEVVSFSDKLN
HHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHCC
WYEKKPLFGRNICITRTKEQAKELKVQLLDLGAEVTEINSIEIKNAEENLKPYLNRLKEY
CHHCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCEEECCEEEECCCHHHHHHHHHHHHCC
DYIALTSVNAVKIFFDYLIKENIDIRNINAKFAAIGPATSEAIKVRGIMPSIKSKHFVAE
CEEEEECCHHHHHHHHHHHHCCCCEEECCCEEEEECCCCCCCEEEEECCCCHHHHHHHHH
SLFEEMKKHVQSGDKVLVPRSKDARPFLVDALKKEGCIVDEVHIYETLCGQSSHTERFED
HHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHCCCEEEHHHHHHHHCCCCCCCCCCCC
ADTVLFTSPSTVKNMISMVGIDSIKEKSIIAIGPITAAELYKNNIECSICDEYSINGIID
CCEEEEECCHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHCCCCEEEECCCCHHHHHH
KLLDLN
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7665501 [H]