Definition Clostridium botulinum A2 str. Kyoto chromosome, complete genome.
Accession NC_012563
Length 4,155,278

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The map label for this gene is 226948179

Identifier: 226948179

GI number: 226948179

Start: 1104154

End: 1104801

Strand: Direct

Name: 226948179

Synonym: CLM_1053

Alternate gene names: NA

Gene position: 1104154-1104801 (Clockwise)

Preceding gene: 226948178

Following gene: 226948180

Centisome position: 26.57

GC content: 29.63

Gene sequence:

>648_bases
ATGAAAGTAATTGATTTAACACATACTATTTCAGAAAATATGCCAGTTTACCCAGGTACAGAAAGACCAAAATTAGAAGT
TAAAAGTACATATGATAAAGATGGTTTTAAAGAAACTTTATTAACTATGTTTTCACATACAGGAACTCATATGGATTCGC
CTGCACATTTATTTCTAGAAAGAACAAATTTAGATTCATTTTCCCCAGAACAATTTGTAGGAAAAGGATTGGTTATTGAC
TGTAGTGATTTAAAAGAAGGTGAAAAAATTCATATGAAATATATTGACTGTGTAAAAGAAAAAGCTAATAAAGCAGATTT
TATTTTGTTTCATACTGGTTGGGATAGGTATTGGGGTACAGATTTTTATTTTGGAGATTATCCTTATATTACAGAAGAGG
TTGCTGAATATCTAATATATAATAAAAAAAAGGGCGTTGGAGTAGATGTTATAGGTATTGATCCTATTTCAGATGAAAAC
TTAACCATTCATCGAAAACTTTTTTTAAAAACTGATATAGTTGTTATTGAAAATCTCACTAGATTAGGGGAAGTTGGAAA
TGAGTTATTTACATTCTGTGCCCTTCCAATAAAATATGAAAATTCTGATGGGGCACCTATTCGTGCAATAGCTATTTTAC
AGGATTAG

Upstream 100 bases:

>100_bases
TTCCTTTGAAAAATCAAATATGGCATATAAAAAAATGTTAACATATATTTATAAACTATATAGCAATAGTAATATATTAT
AAAGAAAGAGAGGTTTTATT

Downstream 100 bases:

>100_bases
TTGCCATAATAATTAAAGTAACATAATGGAGATAATATTTTCTAAAATAGTTTATTTTATTAAGTATCTAAAGTAATATA
GTTAAGTTATAATAGAGAAA

Product: cyclase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MKVIDLTHTISENMPVYPGTERPKLEVKSTYDKDGFKETLLTMFSHTGTHMDSPAHLFLERTNLDSFSPEQFVGKGLVID
CSDLKEGEKIHMKYIDCVKEKANKADFILFHTGWDRYWGTDFYFGDYPYITEEVAEYLIYNKKKGVGVDVIGIDPISDEN
LTIHRKLFLKTDIVVIENLTRLGEVGNELFTFCALPIKYENSDGAPIRAIAILQD

Sequences:

>Translated_215_residues
MKVIDLTHTISENMPVYPGTERPKLEVKSTYDKDGFKETLLTMFSHTGTHMDSPAHLFLERTNLDSFSPEQFVGKGLVID
CSDLKEGEKIHMKYIDCVKEKANKADFILFHTGWDRYWGTDFYFGDYPYITEEVAEYLIYNKKKGVGVDVIGIDPISDEN
LTIHRKLFLKTDIVVIENLTRLGEVGNELFTFCALPIKYENSDGAPIRAIAILQD
>Mature_215_residues
MKVIDLTHTISENMPVYPGTERPKLEVKSTYDKDGFKETLLTMFSHTGTHMDSPAHLFLERTNLDSFSPEQFVGKGLVID
CSDLKEGEKIHMKYIDCVKEKANKADFILFHTGWDRYWGTDFYFGDYPYITEEVAEYLIYNKKKGVGVDVIGIDPISDEN
LTIHRKLFLKTDIVVIENLTRLGEVGNELFTFCALPIKYENSDGAPIRAIAILQD

Specific function: Unknown

COG id: COG1878

COG function: function code R; Predicted metal-dependent hydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007325 [H]

Pfam domain/function: PF04199 Cyclase [H]

EC number: NA

Molecular weight: Translated: 24569; Mature: 24569

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVIDLTHTISENMPVYPGTERPKLEVKSTYDKDGFKETLLTMFSHTGTHMDSPAHLFLE
CEEEEEEHHHCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEE
RTNLDSFSPEQFVGKGLVIDCSDLKEGEKIHMKYIDCVKEKANKADFILFHTGWDRYWGT
ECCCCCCCHHHHHCCCEEEECCCCCCCCEEHHHHHHHHHHHCCCCCEEEEECCCCCCCCC
DFYFGDYPYITEEVAEYLIYNKKKGVGVDVIGIDPISDENLTIHRKLFLKTDIVVIENLT
CEEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEEEEEEECCCHHHHHHH
RLGEVGNELFTFCALPIKYENSDGAPIRAIAILQD
HHHHHHHHHHHHHCCEEEEECCCCCCEEEEEEECC
>Mature Secondary Structure
MKVIDLTHTISENMPVYPGTERPKLEVKSTYDKDGFKETLLTMFSHTGTHMDSPAHLFLE
CEEEEEEHHHCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEE
RTNLDSFSPEQFVGKGLVIDCSDLKEGEKIHMKYIDCVKEKANKADFILFHTGWDRYWGT
ECCCCCCCHHHHHCCCEEEECCCCCCCCEEHHHHHHHHHHHCCCCCEEEEECCCCCCCCC
DFYFGDYPYITEEVAEYLIYNKKKGVGVDVIGIDPISDENLTIHRKLFLKTDIVVIENLT
CEEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEEEEEEECCCHHHHHHH
RLGEVGNELFTFCALPIKYENSDGAPIRAIAILQD
HHHHHHHHHHHHHCCEEEEECCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]